BACKGROUND: Exercise-induced skeletal muscle apoptosis has been referred to as the current focus of sports medicine, and the application of stem cells in athletic injury rehabilitation and prevention has been reported. However, the role of stem cells in cell apoptosis remains unclear.OBJECTIVE: To summarize the effect and mechanism of stem cells in preventing exercise-induced skeletal muscle apoptosis to provide references for scientific sports training and physical activity.METHODS: A computer-based online search of PubMed (1991-01/2009-10) and CNKI (1994-01/2009-10) was performed for related articles with the keywords ""Exercise Training, Sports, Skeletal Muscle, Apoptosis" in English and "stem cells, exercise, skeletal muscle, apoptosis," in Chinese. Inclusion criteria: ① studies on stem cells and their apoptosis in skeletal muscle cells; ②articles in the same field published recently or in the authoritative journals. Exclusion criteria: ① Repetitive articles; ② Meta analysis.RESULTS AND CONCLUSION: A total of 360 articles were collected from the summary of literature through the stem cells and its application in the field of sports medicine research, and its skeletal muscle apoptosis in the changes and development trend of the application. Finally, 31 articles were included, including 21 reviews and 10 clinical or experimental studies. High-intensity exercise can cause apoptosis in skeletal muscle cells, while the use of stem cell technology can prevent apoptosis, to a certain extents by regulating Bcl-2 and Bax protein expression, thereby promoting the early recovery of skeletal muscle.

Objective To study the disinfection effect of ozone on the natural bacteria in indoor air and the central air condition ductwork. Methods Ozonizer was installed on the outlet of central air condition main pipeline and when it began to send breeze, ozone was taken to each branch piping and each room. Then, at the vents of pipeline and in the rooms, the quantity of the natural germs was examined before and after disinfection respectively. The death rate was calculated. Results After 60 minutes of ozone disinfection, the death rate of the natural germs at the vents of central air condition was 60%-90%, that of indoor air was -40.00%-71.43%. Conclusion Ozone can effectively kill the natural germs in ductwork of central air condition, but when the concentration of ozone is lower and there are people in the room, the efficiency will decrease.

This paper analyzes the newest CT and MR products and compares their new technology and clinical application.The development of image inosculation technology is also briefly discussed.

[Objective]To induce TGF-?1 gene which can increase ECM synthesis into adipose tissue derived stem cells(ADSCs) by employing gene transfer techniques and observe whether TGF-?1 gene could expresse continuously and whether type II collagen and aggrecan are synthesized in order to provide experimental data for constructing tissue-engineering cartilage. [Method]ADSCs were transferred with TGF-?1 gene ,TGF-?1,FN,ColⅡ and aggrecan were detected with RT-PCR and TGF-?1 protein was detected with Western-blot.[Result]RT-PCR demonstrated that the expression of TGF-?1,FN,ColⅡ and aggrecan in the TGF-?1 gene transferred groups increased more evidently than non-gene groups and control groups (P

Therapies for malignant glioma include surgery,radiotherapy and chemotherapy.In recent years,the overall effective rate of temozolomide is better than other chemotherapy drugs,but partly patients have resistance to temozolomide.Angiogenesis inhibitors show promising activity,but it is expensive.Celecoxib has antiangiogenic activity,which has become a new option.

BACKGROUND:Many studies concerning influence of traditional Chinese medicine on animal movement capacity have been conducted.However,studies on the improvement of exercise capacity by traditional Chinese medicine are few,especially comparisons among traditional Chinese medicineOBJECTIVE:To explore the influence of different traditional Chinese medicine preparations on the exercise capacity in mice to screen compound preparations with precise effects.METHOD:A total of 80 8-week-old pure male Kunming mice were randomly assigned to 8 groups(n=10):control and compound 1-7 groups.There were no significant differences in body mass of all groups The compound 1 comprised Xuejie and Shexiang;compound 2 comprised Qingui,Baizhu and Baishao;compound 3 comprised Baizhu,Baizhi and Sanqi;compound 4 comprised Baizhi,Ruxiang,Danggui and Radix glycryrrhizae;compound 5 comprised Guiwei;compound 6 comprised Sichuan Honghua and Danshen;compound 7 comprised Danggui and Shexiang.Of the 7 prescriptions,compound 1-5 were empirical formula,compound 6 was common medicine used during training,and compound 7 was prescription of traditional Chinese medicine The 7 compound groupswere respectivelytreatedwith 1.17,0.78,0.78,1.17,7.02,5.53,and 11.70 g/kg per day for 4 weeks,and control group was intragastrically infused with normal saline.From day 4 to 6,load-free swimming training was performed 20 minutes a day for 3 days,followed by swimming for 6 days a week after 1 day of rest.The intensity was gradually increased frOm 30 minutes per day by 10 minutes per day for 2 hours,followed by loading swimming with load of 2%of the body mass,increased by 1%per day until the final experiment.During the entire procedure,a great intensity swimming training was performed every week to make the mice exhausted.Mice training and serum creatine kinase and lactate dehydrogenase 4 weeks following treatment were observed.RESULTS AND CONCLUSION:A total of 80 mice were included in the final analysis The compounds showed little influence on mice body mass;the compound 2 and compound 6 groups had longest exhaustive time All compounds inhibited serum creatine kinase activity following exercise,in particular compound 6 and 2(P＜0.05).Compared with control group,the serum lactate dehydrogenase activity of each compound group was decreased,in particular,compound 2 and 6 groups showed significant differences(P＜0.05).Compound 2 and 6 significantly enhanced the exercise capacity of mice and delayed injury of body.The mechanism may highly relate to components in the compounds,Danggui,Sichuan Honghua and Danshen.

Objective To explore the relationships of serum levels of leptin,TNF-?,FFA and resistin with IR and between themselves. Methods 48 T2DM patients and 47 non-diabetic controls were selected.Levels of leptin,TNF-?,FFA and resistin were measured.FPG,insulin,blood lipid,blood pressure,BMI and WHR were measured.Results The levels of leptin,TNF-?,FFA and resistin were correlated positively with HOMA-IR(P

Objective To study the expressions and significances of matrix metalloproteinases-3 (MMP-3) and tissue-inhibitor of matrix metalloproteinase-3 (TIMP-3) in transitional cell carcinoma of bladder.Methods Immunohistochemistry was used to detect the expressions of MMP-3 and TIMP-3 in 48 cases of transitional cell carcinoma of bladder and 20 normal bladder tissues.Results MMP-3 and TIMP-3 in normal bladder tissues did not express.The more grading and staging,the more MMP-3 expression,but no statistical significance(P ＞0.05),by contrast,TIMP-3 expression decreased and has statistically significant in the tumor grading (P ＜ 0.05).Conclusions High expression of MMP-3 and low expression of TIMP-3 is related to the malignancy of transitional cell carcinoma of bladder and may play an important role in the development of transitional cell carcinoma of bladder,as an important reference to judge the degree of malignancy of transitional cell carcinoma of bladder and prognostic indicator.

Objective To investigate the role of matrix metalloproteinase 2, 9 (MMP 2, MMP 9) and tissue inhibitor of matrix metalloproteinases 2, 1(TIMP 2, TIMP 1 ) in human amniochorionic membrane Methods Amniochorionic membranes were collected from the following groups of women: (1) women with spontaneous premature rupture of membrane (PROM) before onset of labor (PROM group, n =8), (2) Women with term labor after vaginal delivery (vaginal delivery group, n =8), (3) Women undergoing elective cesarean section (C section group, n =8) Messenger ribonucleic acid expression for MMP 2, MMP 9, and their specific inhibitors TIMP 2 and TIMP 1 were studied with reverse transcriptase polymerase chain reaction Results (1)MMP 2 level in PROM group was 0 849?0 037;in vaginal delivery group 0 327?0 023;in C section group 0 307?0 028 Expression in PROM group was highest, with significant difference compared with the other groups ( P 0 05) (2)MMP 9 level in PROM group was 0 026?0 004;in vaginal delivery group 0 008?0 001,with significant difference between the two groups ( P 0 05) Conclusions In PROM group,the expression of MMP 2, MMP 9 and their inhibitors TIMP 2, TIMP 1 is imbalanced, leading to increased extracellular matrix degradation, and weakening of the fetal membranes, and eventually premature rupture of the membranes

Objective To investigate the effects of siRNA against survivin combined with the neoadjuvant chemotherapy (paclitaxel and epirubicin) on the apoptosis in breast cancer MCF-7 cell and reverse drug resistance.Methods Molecular cloning technique was applied to construct the expression vector of siRNA against survivin,and effectene transfection reagent was used to transfect MCF-7 cell.Survivin expression was detected by qPCR and Western blot methods.The effects of paclitaxel or epirubicin combined with or without siRNA against survivin,on the drug susceptibility of MCF-7 cell were detected by CCK-8 assay and that of including MCF-7 cell apoptosis were detected by flow cytometry.Results Survivin-siRNA-2 effectively inhibited the expression of survivin mRNA (0.30±0.03) and protein (0.37±0.09) (P ＜ 0.05).Both paclitaxel [IR 24,48 h =(38.5±4.0) ％,(41.7±6.3) ％] and epirubicin [IR 24,48 h =(37.0±8.6) ％,(40.6± 12.1) ％] can suppress the proliferation of MCF-7 cell and induce its apoptosis,and siRNA against survivin combined with chemotherapy drugs enhanced both effects significantly[paclitaxel IR 24,48 h =(76.0±2.9) ％,(85.1±4.0) ％; epirubicin IR 24,48 h =(74.6±5.6) ％,(82.5±4.8) ％] (P ＜ 0.05).The inhibition rate of MCF-7 was concentration and time dependent.Conclusions Blocking survivin gene expression by RNAi,and combining with the appropriate chemotherapy can significantly enhance the sensitivity of MCF-7 cell to drugs and cell apoptosis.This technology has important potential clinical value in the therapy of breast cancer.