Four methods of statistics and data mining such as regression analysis,principal component analysis,association rules,and cart,chaid,quest decision tree algorithms have been applied to analyze the college nursing students' scores to comprehensively study the nursing course correlation as well as the impact on the students' quality.Combination methods of statistics and data mining,which has complementary advantages,can discover more information such as courses correlation and effect degree of courses on students of different levels etc.Besides,it helps educational administration personnel to set curriculum scientifically,strengthen the communication among the teachers from different department and improve students' study efficiency.

Objective To summarize the clinical experience about video-assisted-thoracoscopic in the treatment of thoracic surgical disease.Methods Through the video-assisted-thoracoscopic technique in the treatment of 282 cases with thoracic surgical diseases,which included the pulmonary bulla resection in 162 cases,the organizing blood thoracic dissection by trauma in 23 cases,the excision of lung-cyst in 17 cases,the empyema clearance and fibreboard stripping in 30 cases,the pulmonary nodular lesion resection in 17 cases,the peripheral lung wedge resection in 13 cases,the spontaneous pneumothorax pulmonary bulla resection and hemostasis hysterectomy in 9 cases,the pleural tumors in 6 cases,the esophageal leiomyoma in 2 cases,the removal foreign body of lung in 3 cases.Results The operative time was 30 ～ 180minutes,the volume of bleed was 20 ～650ml,the postoperative thoracic drainage was 3 ～ 16 days,the postoperative complications occurred in 11 patients,the incidence of complications was 3.9％,there was no death.Conclusion The thoracoscopic minimally invasive surgery is trauma,less bleeding,rapid recovery,and had the broader indications for thoracic surgery.

Objective To explore the diagnosis and trealanent of closed tracheal,bronchial injury.Methods The clinical features,early diagnosis and treatment about 7 cases of closed treacheal,bronchial injury wrerarolyzed.Results 3 cases of tracheal injury in neck were cured after urgent repairment:1 case of membranous tracheal chest injury was cured after drainage about mediastinal;the 3 cases of tracheobronchial injury were cured by sutured,patched and formed.Conclusion The key measure of increase the cure rate,reduce complications and reduce morality is early diagnosis and timely-reasonable treatment.

Objective To investigate the expression of homocysteine and cystatin C in the serum of patients with different types of malignant tumors.Methods 120 patients with malignant tumors were divided into group A (gastric cancer),group B (esophageal canler),group C (liver cancer) and group D (nasopharyngeal carcinoma) according to the type of disease,25 healthy volunteers were selected as control group.Enzyme-linked immunosorbent (ELISA) was used to measure serum homocysteine and cystatin C levels in four groups of patients.Results Homocysteine level of group A,group B,group C and group D were (19.71 ± 3.40)μmol/L,(23.27 ± 9.57)μ mol/L,(21.37 ±0.38) μmol/L and (16.27 ± 1.24) μmol/L,which were significantly higher than those of the control group (all P ＜ 0.05).Cystatin C levels of group A,group B,group C and group D were (19.71 ± 3.40) mg/L,(19.71 ±3.40) mg/L.(19.71 ± 3.40) mg/L and (19.71 ± 3.40) mg/L,which were significantly higher than those of the control group (all P ＜ 0.05).Serum homocysteine and cystatin C levels of gastric cancer positively correlated with carcinoembryonic antigen (CEA) level(r =0.9643,0.9842).However,serum homocysteine and cystatin C levels of esophageal cancer,liver cancer and nasopharyngeal carcinoma had no correlation with serumCEA level.Conclusion Cystatine C and homoeysteine have potential clinical value on the diagnosis of malignant tumors.

Objective To observe the clinical curative effect of Bushen Huoxie Paidu decoction in delaying the renal fibrosis progression and retarding the course of chronic renal failure (CRF). Methods Seventy-six patients with CRF were randomly divided into the test group (38 cases) and the control group (38 cases). Both groups were treated with relatively unified fundamental treatment, experienced prescription of Bushen Huoxie Paidu decoction was given additionally to the test group. Treatment course of each group was 3 months. The curative effect was observed and the plasma levels of laminin (LN), fibronectin (FN), procollagen type Ⅲ (PC-Ⅲ) and collagen type Ⅳ (Ⅳ-C) in both groups were determined. Results The statistic analysis showed that the total effect of two groups (81.58% and 55.26%) had a significant difference (P

BACKGROUND: It is believed that repetitive transcranial magnetic stimulation (rTMS) may produce such neurophysiological effects as regulating regional cerebral blood flow, neurotransmitters, local metabolism, and neuronal remodeling after nerve tissue injuries. The prognosis ofischemic stroke is related with the cortical function reconstruction in the ipsilateral and contralateral hemisphere of the lesion. Currently studies have not defined whether rTMS can affect the cortical function, protect ischemic neurons and promote motor functional recovery after cerebral ischemia.OBJECTIVE: To investigate the effects of rTMS on rat motor cortical excitability and neural function in acute stage of cerebral ischemia-reperfusion injury.DESIGN: Completely randomized experiment.SETTING: Electroneurophysiological Laboratory of Peking Union Medical College Hospital.MATERIALS: The experiment was completed in the Zoological Research Center of Peking Union Hospital from January to June 2004. Totally 22adult male healthy Wistar rats were randomly divided into treatment group and the control group with 11 in each.METHODS: After determination of the average motor threshold of the right hind limbs, which was 22% of the maximum output, the rats were subjected to middle cerebral artery occlusion for 1 hour followed by reperfusion for 72 hours. At each time point of immediately and at 12, 36 and 60 hours after the initiation of reperfusion, the rats in the treatment group received rTMS treatment (20 Hz, 40% maximum output, 5 seconds for each session with an between-session interval of 2 minutes for a total of 10 sessions), and the site for motor threshold evaluation was used for rTMS stimulation; the rats in the control group recevied no treatment after model establishment. Motor threshold testing was performed in both groups 4 hours after the last session of treatment to avoid immediate-early effects of rTMS on the motor threshold. At 24 and 72 hours of reperfusion, the scores of neural function were recorded according to evaluation systems. All the rats with scores between 1 and 3 were enrolled in statistical analysis were evaluated between.MAIN OUTCOME MEASURES: ① Motor threshold of the rats in both groups before and after injury; ② Neural function scores at 24 and 72 hour reperfusion; ③ Infarct volume at 72 hour of reperfusion.RESULTS: Totally 13 rats entered the final result analysis. Before injury,motor threshold in the treatment and control group was similar (P=0.71),and after the injury, the motor threshold of the control group was 1.49times that of the treatment group but such difference was not statistically significant [(41.62±24.73)% vs (28.00±9.35)%, t=-1.17, P=0.27]. At 24hours of reperfusion, the functional scores of the treatment group and control group were not significantly different (P=0.46), but at 72 hours, the scores of the treatment group were significantly lower than that of the control group (1.60±1.52 vs 7.75±3.62, t=-3.57, P=0.004). The average infarct volume of two groups was (62.00±60.88) mm3 and (20.00±12.41) mm3 at 72 hours of reperfusion, respectively, which, after logarithm transformation,was not significantly different between the two group (t=-1.31, P=0.22),but when the infarct volume was transformed into Log10 values, a significant difference occurred between them (P=0.045).CONCLUSION: rTMS may stabilize and prevent the increment of the motor threshold, time-dependently relieve the neural function disability and reduce the infarct volume after cerebral ischemia-reperfusion injury.

Therapies for malignant glioma include surgery,radiotherapy and chemotherapy.In recent years,the overall effective rate of temozolomide is better than other chemotherapy drugs,but partly patients have resistance to temozolomide.Angiogenesis inhibitors show promising activity,but it is expensive.Celecoxib has antiangiogenic activity,which has become a new option.

Objective　To improve the recognition of the imaging appearances of inflammatory pseudotumors of the spleen (IPS), 3 cases with IPS were reported. Methods　The US (n=3), CT (n=3) and MRI (n=1) findings of IPS were reviewed and correlated with the pathologic findings. Results　On US, a well-defined solitary mass with heterogeneous echo texture was found in all 3 cases. A hyperechoic rim with associated acoustic shadowing was shown in 1 case. In all of the 3 cases in nonenhanced CT scanning, a well-defined hypoattenuated mass was found. One had a calcified egg-shell-like rim; On the venous/delayed phase of enhanced CT after contrast administration in 2 cases, slight/marked enhancement was shown. On nonenhanced MRI in 1 case, the mass was shown as heterogeneous hypointensity on T1- and T2- weighted images. Conclusion　IPS should be included in the differented diagnosis of solitary mass lesion of spleen. The imaging findings depend on the variable proportions of fibrous and granulomatous components within the lesion. IPS was characterized by well-defined solitary mass on sonogram, delayed enhancement on enhanced CT, and hypointensity on T2 weighted MR images.

Objective To explore the clinical value of examining HPV E6/E7 mRNA level in assessing cervical le?sions infected with high-risk human papillomavirus (HR-HPV). Methods The cervical epithelial cells were collected from 265 patients with HR-HPV infection, including 100 cases of neoplasia free/inflammation group (control group), 88 cas?es of cervical intraepithelial neoplasia (CIN)Ⅰ, 33 cases of CINⅡ, 28 cases of CINⅢand 16 cases of cervical carcinoma and the transcription of HPV E6/E7 mRNA level was examined using branched DNA (b-DNA) technology. Results The positive rate HPV E6/E7 mRNA were higher in CIN Ⅱ(81.82%), CINⅢ(89.29%) and cervical cancer group (100.00%) than tthat in control group (20.00%) and CINⅠ(35.23%) with significant difference, and there were no significant differences between other groups;The positive rate and transcription level of HPV E6/E7 mRNA in HSIL (high grade squamous intraepi?thelial lesion)and cancer group were significantly higher than normal, ASC(atypical squamous cell carcinoma) and LSIL(low grade squamous intraepithelial lesion) group (P<0.05). Conclusion The transcription level of HPV E6/E7 mRNA may re?flect the activity of the virus and the progression of disease, and could be use as an effective indicator to screen high grade cervical pathological changes and a complementary method of cervical lesion screening.

Objective To investigate the relationship between the amplification of human telomerase reverse tran-scriptase (hTERT) gene and high-risk human papillomavirus (HR-HPV) infections in cervical intraepithelial neoplasia (CIN) and cervical carcinoma. Methods The cervical epithelial cells were collected from 34 samples of normal cervical epithelium, 31 samples of CIN (gradeⅠ), 33 samples of CIN (gradeⅡ), 34 samples of CIN (gradeⅢ) and 20 samples of cer-vical carcinoma. HPV DNA was detected by polymerase chain reaction-reverse dot blot hybridization (PCR-RDB) and the amplification of hTERT gene was detected by fluorescence in situ hybridization (FISH). Results Twenty subtypes of HR-HPV were detected including HPV16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 67, 68, 69, 73 and 82. The inci-dence of HR-HPV infection was higher in CINⅡgroup (72.73%), CINⅢgroup (85.29%) and cervical carcinoma group (90.00%) than that of normal cervical epithelium group (20.59%). There was no significant difference in the positive rate of HR-HPV DNA between CINⅠ group (54.84%) and normal cervical epithelium group (P < 0.005). The positive rate of hTERT gene amplification was higher in cervical carcinoma group (80.00%) than that of normal cervical epithelium group (0). There were no significant differences in the positive rates of hTERT gene amplification between CINⅠgroup ( 3.22%), CIN Ⅱ group (18.18%), cervical carcinoma group and CIN Ⅲ group (41.18%). There was positive correlation between hTERT gene amplification and HR-HPV infection (r=0.238, P<0.05). Conclusion The incidence of HR-HPV infection was positively correlated with hTERT gene amplification in cervical lesions. HR-HPV infection may be an early event of ab-normal amplification of hTERT gene. The detection of HPV-DNA and hTERT gene can be used in the clinical diagnosis of early cervical lesions.