Objective To study the relationships between sperm morphology and sperm vitality,sperm viability.Methods The sperm morphology and the sperm vitality were analyzed by automated sperm morphology analyzer(ASMA) and the sperm viability was analyzed using eosin staining.Results The normal morphology sperm rate of abnormal vitality group was lower than that of normal vitality group(P

Objective: To explore whether relieving effect of Ginsenosides-Rb1 (Gs-Rb1) on chronic heart failure (CHF) induced by adriamycin is related to improving levels of tumor necrosis factor (TNF) receptor (TNFR) and ligand TNF-α or not. Methods: Adriamycin-induced CHF model rats were randomly divided into adriamycin group (n=15, received adriamycin 1 μmol/L) and Gs-Rb1 group (n=17, received Gs-Rb1 70 mg•kg-1•d-1 based on adriamycin group), another 10 healthy Wistar rats were selected as normal control group. Meanwhile, cultured cardiomyocytes of neonatal rats were accordingly divided into normal control group, adriamycin group and adriamycin+Gs-Rb1 group. After intervention, echocardiography, levels of TNFR/TNF-α mRNA and protein were measured and compared among three groups. Results: 1. In vivo: Compared with normal control group TNFR-1 [protein（0.13±0.02）kDa, mRNA（0.19±0.05）bp]，TNFR-2[protein（0.24±0.01）kDa, mRNA（0.13±0.02）bp]，TNF-α [protein（0.11±0.01）kDa, mRNA（0.26±0.05）bp], the levels of TNFR-1 [protein（0.67±0.04）kDa,（0.51±0.04）kDa；mRNA（0.81±0.03）bp，（0.49±0.05）bp]，TNFR-2 [protein（0.61±0.05）kDa, （0.47±0.03）kDa，mRNA（0.28±0.03）bp，（0.18±0.04）bp] and TNF-α [protein（0.28±0.04）kDa, （0.20±0.03）kDa， mRNA（0.67±0.05）bp，（0.45±0.04）bp] significantly rose（P<0.05 or <0.01） in adriamycin group and Gs-Rb1 group; and those levels of TNFR-1, TNFR-2, TNF-α protein and mRNA of Gs-Rbl group were significant reduction than those of adriamycin group; 2. In vitro: The results were similar, those of adriamycin group and Gs-Rb1 group were also significant rise than those of normal control group（P<0.05 or <0.01），those of Gs-Rbl group were significant reduction than those of adriamycin group（P<0.05 or <0.01）. Conclusion: TNFR/TNF-α expression may play a certain role in the process of adriamycin-induced CHF; improving effect of Gs-Rb1 on adriamycin-induced CHF may be related to regulation of TNFR/TNF-α.

Objective To investigate the effect of miRNA-1 on the cardiomyocyte apoptosis in rats. Methods MicroRNA-1 mimics was transfected into the cultured H9c2 cell line (miRNA-1 group). Cells transfected with random miR-NA fragment was used as negative control group. The cell apoptosis was evaluated by FCM assay. MTT assay was used to de-tect the cell viability. The expression level of miRNA-1 was detected by real-time PCR. The expression levels of Bcl-2 mRNA and protein were detected by real-time PCR and Western blot assay. Results Compared with normal and negative control groups, the expression level of miRNA-1 was significantly higher in H9c2 cardiomyocytes, the apoptosis rate was in-creased, the cell vitality and Bcl-2 expression level were significantly decreased after transfection of miRNA-1 mimics. Conclusion miRNA-1 mimics can up-regulate miRNA-1 level, inhibit proliferation and induce cardiomyocyte apoptosis.

Objective To investigate the relationship between homocysteine(Hcy)and blood uric acid(UA)in the patients with type 2 diabetes mellitus(T2DM)complicating hypertension.Methods 85 patients with T2DM complicating hypertension were se-lected as the groupⅠ,78 patients with simple T2DM as the group Ⅱ and 74 individuals with healthy physical examination as the group Ⅲ.Hcy,UA,TC,TG and HDL-C were measured in all the subjects.Results Compared with group Ⅲ(control),the levels of Hcy,UA,TC and TG in the group Ⅰand groupⅡwere significantly increased(P <0.05),but the level of HDL-C was significantly decreased(P <0.05).Compared with the group Ⅱ(T2DM),the levels of Hcy,UA,TC and TG in the group Ⅰwere significantly in-creased(P <0.05 ),but the level of HDL-C was obviously decreased,the difference had statistical significance (P <0.05 ).There was good positive correlation between the Hcy level and the UA level in the patients with T2DM complicating hypertension (r =0.658,P <0.05).Conclusion The levels of Hcy and UA are significantly increased in the patients with T2DM especially complica-ting hypertension.High Hcy and high UA are the important risk factors of T2DM complicating cardiocerebrovascular diseases.

Seawater,hyperosmotic and alkaline, is a complex wound factor. Specific pathophysiological changes appear when wounds are socked in seawater. and special treatments are needed for such injuries. This paper reviewed the pathophysiological changes and treatment of seawater-soaked soft tissue wound, cranioccrebral trauma, open chest trauma,open ahdom-inal trauma,sea water drowning and seawater immersion during shock.

Objective To investigate the clinical autopsy results of patients died of cardiovascular disease or other disease complicated with cardiac damage. Methods Complete autopsy was performed on 86 cases with uncertain cause of death. Through integrating clinical diagnosis and treatment with gross autopsy findings and microscopic observations, 86 autopsies were determined the major cause of death. Results In 86 autopsies, 69 cases were heart disease. Differences between pathological diagnosis and clinical diagnosis were compared. Twenty-seven cases were cardiac deaths, with diagnosis accrodance rate of 81. 5%. Fortytwo cases died of non-cardiac disease but complicated with heart disease or involving the heart which accelerated the death in patients, with accordance rate of 78.6%. Conclusion Scientific and correct performance of autopsy was important to determine the causes of death, to promote development of related disciplines and to improve the diagnosis and treatment of the diseases.

Images of 812 patients undergoing dual-source CT coronary angiography were analyzed retrospectively. Effectively nursing can be improved to guarantee the quality of coronary angiography and diagnosis of diseases.

Objective To explore the diagnostic value of "Standard" EEG,which include common induced test with sleeping induce(1.5 to 2.0 h) and choice sphenoidal electrode description in epilepsy.Methods 236 epileptic patients were inspected with routine,standard EEG early and late, and then they were diagnosed primarily and once more.Results The epileptic discharges positive rate(81.36%) of standard EEG was higher than routine EEG (29.66%),(P

Objective To study the clinical value of combined detection of D-dimer,PT,FIB and Cyc-C in the patients with nephrotic syndrome(NS) complicating thromboembolism.Methods Sixty-five patients with clinically diagnosed NS(observation group) in the Huangshi Municipal Central Hospital from April 2013 to March 2016 were selected and divided into the non-thromboembolism group(group A,45 cases) and thromboembolism group(group B,20 cases).Contemporaneous 43 individuals undergoing physical examination served as the control group(group C).The levels of Cys-C and 24 h urine protein were tested by the electrochemical luminescence method;meanwhile PT,FIB and D-dimmer levels were detected by the nephelometry method.Their changes in the patients with NS complicating thromboembolism were observed.Results The levels of Cys-C,24 h urine protein and D-dimer in the observation group were significantly higher than those in the control group (P＜0.01).The difference of PT and FIB levels were not statistically significant(P＞0.05);the D-dimer and Cys-C levels in the observation B group was significantly higher than that in the observation A group(P＜0.05),moreover the D-dimer level was positively correlated with Cys-C.Conclusion D-dimer and Cys-C can be used as the predictive factors of thromboembolism occurrence in NS patients.

Objective To observe the effects of solanine on DNA and RNA level in tumor cell of S_(180) and H_(22) tumor-bearing mice.Methods S_(180) and H_(22) mice were divided into solanine(37.50、18.75,and(9.37) mg/kg) groups,negative control group and cytoxan(30 mg/kg) group,whom were given drugs by sc.Levels of RNA and DNA in tumor cell membrane in every groups were examined,respectively by laser scanning confocal microtechnic technology.Results Solanine(37.50、18.75 mg/kg) groups reduced the ratio of RNA and DNA in tumor cell of both S_(180) and H_(22) mice.Conclusion Solanine can reduce the ratio of RNA and DNA in tumor cell of S_(180) and H_(22) mice,which may be one of the mechanisms of solanine's(antitumor) effect.