Objective:To evaluate the effects of domestic recombinant adenovirus p53 injection (rAd-p53,Gendicine) combined with cisplatin (DDP) on growth and apoptosis of ovarian carcinoma cell lines. Methods:MTT assay, flow cytometry, and Western blotting were used to compare the effects of rAd-p53, DDP and their combination on the growth, cell cycle, apoptotic rate, and p53 protein expression of ovarian carcinoma cell lines. Results:rAd-p53 inhibited ovarian carcinoma cells proliferation in dosE- and timE-dependent manners. After combined treatment, the growth inhibition rate was significantly increased (P<0.01). There was no significant diffE-rence in the inhibition potency induced by combined treatment with different sequences (P>0.05). Combined treatment significantly arrested ovarian carcinoma cell lines in G0/G1 phase at 72 h, the cell number in S phase was significantly decreased and the apototic rate was significantly increased (P<0.01). High expression of p53 protein was detected in ovarian carcinoma cell lines at 72 h after administeration of rAd-p53. Conclusion:rAd-p53 tranfects the exogenous widE-type p53 into the genome group of human ovarian tumor cells, induces p53 protein expression, thereby arresting tumor cells at G0/G1 phase, inhibiting tumor growth, and inducing apoptosis.

0.05).Significant differences were found tetween preoperation versus postoperation in both groups(P

Objective To evaluate the clinical efficiency of uterine artory embolization for uterine fibroids. Methods From August 1999 to June 2001, 405 cases were included in the treatment by using UAE and were followed up to find its efficiency. Results The successful rate of catheterization almost reached 99.8% (2/405). B mode ultrasound examination following 3～12 months after the procedure re vealed an average shrinkage of 40%～60% in volume of the masses for all cases in the first 3 months and 50%～70% in volume in 6 months, with 22 UF disappeared together with conspicuous decrease in volume of menstruation. Conclusions UAE for UF is a new, safe and effective method

Objective To evaluate the effects of magnesium sulphate on the efficacy of postoperative patient-controlled intravenous analgesia (PCIA) with morphine. Methods Sixty ASA Ⅰ or Ⅱ patients of both sexes aged 45-60 yr weighing 48-70 kg scheduled for elective upper abdominal surgery were randomly allocated into 2 groups ( n = 30 each) : morphine group (group M) and magnesium sulphate-morphine group (group MS) . If the VAS score=3, PCIA was started. Patients in group M received morphine in a 0.015 mg/kg bolus dose. Patients in group MS received morphine 0.015 mg/kg and magnesium sulphate 0.9 mg/kg. The consumption of analgesic drugs was recorded at 4, 8, 16, 24 and 48 h after 1st attempt. The gastrointestinal function recovery time was recorded. Venous blood samples were taken before anesthesia induction and at the end of operation and analgesia to determine the serum magnesium and calcium ion concentrations. Results Compared with group M, the consumption of analgesic drugs was significantly decreased, and the gastrointestinal function recovery time after operation was significantly shortened in group MS (P < 0.05). The serum magnesium ion concentration in both groups was significantly lower at the end of operation and analgesia than before anesthesia induction ( P < 0.05) . The serum magnesium ion concentration in group MS was significantly higher at the end of analgesia than at the end of operation ( P <0.05) .There was no significant difference in the serum calcium ion concentration between M and MS groups. Conclusion Magnesium sulphate can ameliorate the efficacy of postoperative PCIA with morphine.

Objective:To explore strategies of improving patients' stereotyping of nurses, by analyzing patients′ stereotypes of nurses.Methods:The phenomenological analysis method in qualitative research was used to conduct semi-structured interviews with 12 inpatients by objective sampling method.Results:The patients′ stereotype of nurses could be summarized into three topics, it mainly consisted of three aspects: patients′ cognition of the role of nurses, patients′ stereotype of nurse-patient relationship and factors affecting nurse-patient relationship from patients′ perspective, among which factors affecting nurse-patient relationship included doctors′ attitude towards nurses, public opinion effect of media reports and patients′ negative psychological experience.Conclusions:The prejudice of patients to the relationship between nurses and patients is not optimistic, it is suggested to improve patients′ stereotyping of nurses by reconstructing the ideal role of nurses, building harmonious trusting relationships and providing friendly support from the "others".

Objective To evaluate the prevalence,anatomic features of right superior septal artery (RSSA) with 256-slice MSCT.Methods A retrospective analysis of coronary artery computed tomography angiography with 256-slice CT was performed in 1 646 consecutive patients.Multi-planar reconstruction (MPR),maximum intensity projection (MIP) images on coronal and sagittal planes,and three-dimensional volume rendering (VR) reconstruction images were obtained and used for the evaluation of the anatomic features of the RSSA.The images were transferred to EBW4.52 workstation to trace the vessel and to analyze the origin,diameter,and length of the RSSA.Student's t test was performed to compare the differences in the length and diameter of the RSSA between patients with different coronary artery distribution dominant types,different genders.Analysis of variance (ANOVA) was used to compare the differences in the length and diameter of the RSSA among patients with and without coronary artery stenosis.Results The RSSA was present in 130 (7.9％) of 1 646 patients.The origin of RSSA was from the proximal portion of the right coronary artery in 104 patients,from the right sinus of valsalva in 26 patients.The artery co-existed with the conus artery in 22(16.9％) of 130 patients.The mean length of RSSA was (31.7±15.6) mm (range from 8.9 to 70.7 mm),and the mean diameter was (1.0±0.4) mm (range from 0.2 to 2.5 mm).The average length and diameter of RSSA in men were (33.5±15.7) and (1.0±0.4) mm,respectively; The average length and diameter of RSSA in women were (24.5 ± 13.0) and (0.9 ±0.4) mm,respectively.There was a significant difference in RSSA length between men and women (t=2.718,P=0.007),but there was no significant difference in the RSSA diameter between men and women (t=1.134,P=0.259).There was no significant differencein RSSA length and diameter between different coronary artery distribution dominant types (t=-0.219 and-0.080 respectively,P＞ 0.05).In the patients with left anterior descending artery (LAD) and right coronary artery (RCA) stenosis,the mean length and diameter of RSSA were (38.9±17.9),(1.1 ±0.4) mm,respectively.In the patients without LAD and RCA stenosis,the mean length and diameter of RSSA were (28.9±14.4),(0.9± 0.4) mm,respectively.Patients with coronary artery stenosis tended to have longer RSSAs in comparison to those without coronary artery stenosis (P＜0.05).Conclusions RSSA variantions can evaluated with a cardiac 256-slice MSCT scan.The recognition of this vessel is useful for physians dealing with diagnosis and treatment of coronary artery disease.

Objective To analyze clinical effects of gastric tube operation for treatment of esophageal cancer in elderly patients and evaluate its clinical efficacy and safety.Methods A total of 171 patients aged 60-72 years with esophageal cancer in our hospital were selected.They were randomly divided into treatment group and control group.Treatment group was treated with gastric tube operation,and control group was traditionally treated with complete replacement of esophagus with stomach.The quality of life,patient satisfaction and safety of operation were evaluated after 3 weeks,6 months and 12 months after operation,respectively.Results The operation for esophageal cancer in 171 patients were successful.At 3 weeks after the operation,the score of life quality in treatment group and control group were both low [(67.3±9.6) vs.(65.3±8.4)],and there were no significant differences between the two groups (P＞0.05).At 6 months and 12 months after operation,the scores of life quality were (89.2±8.3) and (90.3±9.6) in treatment group,and (66.5± 10.4) and (60.5 ± 11.2) in contol group,respectively.There were statistical differences between the two groups after 6 months and 12 months of operation (P＜0.05).The complication rate in treatment group (6.9％) was much lower than that in control group (30.6％),and there were significant differences between the two groups (x2 =18.43,P＜0.01).The patient satisfaction was in no differences between the two groups at 3 weeks after operation (P＞ 0.05),while there were statistically significant differences at 6 and 12 weeks after the operation (P＜0.05).Conclusions Gastric tube operation in treatment of elderly patients with esophageal cancer,can effectively improve the life quality,and prevent the occurrence of postoperative complications,which is worthy of clinical application.

Gastric cancer incidence is one of the most common malignancies in our country and is the second most common in the worldwide,clinic doctors always emphasize early diagnosis and treatment of gastric cancer patients,in order to reduce the mortality,however,most patients' condition often have been in the late fall and these patients were badly in efficacy.Looking for a new diagnosis way is a medical prddem,with molecular biology advance and gene chips was improved,it is possible for the early screening of gastric cancer.This assay aims to briefly analyse the role of gene chips in the research progress of early gastric cancer.

10.3969/j.issn.1000-8179.2013.12.004

The condition of birth defects in our country is very serious,which impose an enormous burdenon the families and society.Under the vision of care ethics,this paper emphasizes understanding and caring,analyzes the ethical dilemma in the prevention of birth defects and puts forward the prevention measures in line with the codes of ethics,aiming at strengthening the practical effect of birth defects prevention work,caring about the physical and mental health of child-bearing female,and promoting social development.