Objective:To separate and purify polysaccharide MP-Ⅰ from Mytilus coruscus and to investigate its in vitro anti-tumor activity.Methods: The crude polysaccharide was extracted from Mytilus coruscus with hot water and the protein was removed using Sevage method.The polysaccharide MP-Ⅰ was purified using DEAE-Sepharose and Sepharose CL-6B column chromatography.Monosaccharides analysis was carried out using thin-layer chromatography and gas chromatography.The in vitro anti-tumor activity of MP-Ⅰwas assessed using MTT method.Results: The yield of MP-Ⅰwas 2.14%.MP-Ⅰ is mainly constituted by glucose.MP-Ⅰ(at 0.5,0.1,0.02 mg/ml) had different degrees of inhibitory effects on HO-8910,MCF-7, K562,and SMMC-7721 tumor cells in vitro(P

AIM:To study the difference of the chemical constituents between raw Radix Aucklandiae and Radix Aucklandiae roasted with wheat bran.METHODS:UV,HPLC and TLC were performed to analyze the chemical constituents before and after the processing.The constituents absorbed by wheat bran during the processing A.lappa.were isolated by silica gel CC.Their structures were identified by spectral analysis.RESULTS:The result of UV,HPLC and TLC showed that change of the chemical constituents in after the roasting had taken place.Two major chemical constituents absorbed by wheat bran were dehydrocostus lactone and costunolide.CONCLUSION:The difference in sesquiterpeneoide before and after the processing of Radix Aucklandiae may be the evidence that the roasting with bran is to the benefit of therapeutic effect.

AIM:To study the difference of the chemical constituents between raw Radix Aucklandiae and Radix Aucklandiae roasted with wheat bran.METHODS:UV,HPLC and TLC were performed to analyze the chemical constituents before and after the processing.The constituents absorbed by wheat bran during the processing A.lappa.were isolated by silica gel CC.Their structures were identified by spectral analysis.RESULTS:The result of UV,HPLC and TLC showed that change of the chemical constituents in after the roasting had taken place.Two maior chemical constituents absorbed by wheat bran were dehydrocostus lactone and costunolide.CONCLUSION:The difference in sesquiterpeneoide before and after the processing of Radix Aucklandiae may be the evidence that the roasting with bran is to the benefit oftherapeutic effect.

Objective To evaluate the effects and possibility of nitroglycerin on Pregnancy induced hypertension (PIH) treatment Methods In comparision with the third trimester normal pregnant women (Normal group) (n=11), 34 moderate/severe PIH cases were divided into three groups: PIH treated only with magnesium sulfate (Mg group) (n=11); PIH treated only with nitroglycerin (Ng group) (n=11); and PIH treated with Mg+ Ng (Combination group) (n=12). The endothelial nitric oxide synthase (eNOS) in placenta was investigatd immunohistochemiscally and quantitatively. Data such as platelet aggregation (PAg), umbilcal artery of S/D ratio and resistance index (RI) with color Doppler and the effect of decrement of blood pressure on PHI groups were evaluated. Results In comparision with Normal group, (1) the quantity of eNOS of PIH cases was signaficently lower (P

Objective:To assess the impacts of feeding type and non-nutritive sucking activity on occlusion in deciduous dentition in WeiFang city.Methods:The occlusion of 958 children aged 3 -5 years old in WeiFang city and their feeding types as well as the non-nutritive sucking habit during the first 1 2 months after birth were investigated by questionaire study.Data were statistically ana-lysed.Results:The prevalence of malocclusion in non-nutritive sucking habit group and non-habit group was 42% and 22% respec-tively(P =0.000 2).The type of feeding did not have effect on occlusion and openbite.The prevalence of openbite in the children with sucking habit and in those without habit was 17% and 4% respectively(P <0.000 1 ).The prevalence of posterior crossbite in the chil-dren with bottlefeeding and those with breastfeeding was 1 1 % and 4% respectively(P =0.000 2).The prevalence of posterior crossbite in the children with sucking habit and those without habit was 9% and 4% respectively(P =0.036 7).Conclusion:Non-nutritive sucking habit rather than feeding type in the first 1 2 months after birth is the main risk factor of malocclusion,breastfeeding is the pro-tective factor to occlusion.

The relationship between blood pressure and various risk factors were investigated in 54 cases of non-insulin-dependent diabetes mellitus (NIDDM) with stepwise regression analysis. After evaluating the influence of age, course, body mass index (BMI), fasting and postprandial blood glucose, serum IRI, C-peptide, HbA1c, plasma lipids (Ch, TG, HDL-C. LDL-C). ApoA1, ApoB2, 24h urine Alb/Cr, NAG/Cr. TRF Cr and RBP/Cr levels on blood pressure, the following results were found: 1) NIDDM patients had a two-fold increased risk of hypertension than normal population. 2) Among the 21 risk factors analysed, 6 variables were strongly correlated with blood pressure, i. e. , age, postprandial blood glucose, serum IRI, blood total cholesterol, 24h urine Alb/Cr and TRF/Cr level. 3) Postprandial hyperglycemia was the most important variable positively correlated with hypertension, followed by 24h urine Alb/Cr and plasma total cholesterol.

Through the Introduction of the visualization technology based on VREL and its application in ultrasound teaching,the teaching means has been changed,the teaching content enriched and the contact between basic and clinic knowledge enhanced,which indicates that the visualization technology exerts an important role in ultrasound teaching.

Objective:To establish an Ehrlich reaction-colorimetry method for determining furostanol saponins in saponins extract from Radix Asparagi.Methods: Based on the coloration reaction between furostanol saponins and p-dimethylaminobenzaldehyde,a colorimetry method was established to determine the content of furostanol saponins in saponins extract from Radix Asparagi at 510 nm.Saponins AC-9 was measured as reference in the determination.Results: The standard curve of saponins AC-9 showed good linearity in the range of 0.08-0.40 mg and the average recovery was 99.79%(RSD=(1.05%).) Conclusion: This method is rapid,accurate and easy to be carried out,and it can be used to control the quality of Radix Asparagi.

Objective To investigate the expression of membrane CD14 (mCD14), Toll-like receptor (TLR4) and nuclear factor-kappa B (NF-kB) in peripheral blood ieukocytes from patients with psoriasis vulgaris (PV). Methods Blood samples were obtained from 29 normal controls and 32 patients with PV. Patient cohort consisted of 19 males and 13 females, with an average age of 30.28 ±13.11 years, disease duration of 8.21±10.21 years, and PASI score of 21.19±5.85. Flow cytometry was used to measure the expression of mCD14, TLR4 and activated NF-kB in peripheral blood leukocytes in these subjects. Results The expressions of mCD14 and TLR4 in peripheral blood leukocytes were significantly higher in patients than in the normal controls (5.454±2.78 vs 3.937±1.964, P < 0.05; 17.641±18.120 vs 7.527±8.574, P <0.01 ). Although the expression of activated NF-kB in total blood leukocytes in patients was higher than that in normal controls, no statistical difference was noticed (63.538±8.650 vs 62.236±6.72, P > 0.05). Conclusion There is an abnormal expression of mCD14 and TLR4 in patients with PV, suggesting that the abnormal activation of endotoxin signal transduction pathway may exert a role in the induction of psoriasis.

Objective To study the biocompatibility of bone marrow mesenchymal stem cells (BMSCs) on acellular collagen matrix double-layer material.Methods BMSCs with acellular collagen matrix double-layer material were trained as experimental group,while separately cultured BMSCs as control group.The growth and proliferation of BMSCs on acellular collagen matrix double-layer material were investigated.Scanning electron microscope was used to observe the adherence of cells,and cell vitality was detected by CCK-8 method.Results The acellular collagen matrix double-layer material did not influence the growth and proliferation of BMSCs.The difference between the growth curves of experimental group and control group was not significant (P＞0.05).Conclusions The acellular collagen matrix double-layer material has acceptable biocompatibility.This research provides a scientific basis for the application of this composite material.