Objective To investigate the relationships between expression of FAK and Prostate Carcinoma (PC)morbidity. Methods By surgery,get cancer tissue and para-carcinama tissue from 60 cases PC.To detect FAK expression by immuno-histochemical.To extract total RNA by Trizol.To detect the FAK mRNA by RT-PCR,and analysis these data.Results FAK expression level in cancer tissue was higher than that in para-carcinama tissue.There was statistical difference between them (χ2=72.55,P<0.01).mRNA expression levels of FAK in cancer tissue showed significant higher than the levels in para-carcinama tissue (t=30.51,P<0.01).According to lymphatic metastasis,the expression positive cases of FAK in pN0M0 classification were lower than these in pN3M1 classification,and mRNA expression levels of FAK were the same re-sults.There were clearly statistical distinctive (t=25.43,P<0.01).Conclusion The expression of FAK in PC cells was as-sociated with tumor invasion.

AIM To investigate the effects of D galactose on bone contents of hydroxyproline(HOP), calcium, microelements and activities of antioxidation in mice. METHODS Twenty female kunming mice at three months of age were used in this study. D galactose at dose of 1 g?kg -1 ?d -1 was given subcutaneous injection daily to the mice for 42 days. The right femurs were collected to determine the bone dry weight, bone hydroxyproline content, bone calcium, and bone microelements. The activities of catalase (CAT), glutathione peroxidase (GSH Px) and superozide dismutases (SOD) in blood, and contents of methylenedioxyamphetamine (MDA) in serum, lipofuscin in liver were determined. RESULTS The bone dry weight, hydroxyproline, calcium of bone decreased significantly in D galactose treaded group(compared with control group, P

OBJECTIVE To understand the related factors,the situation and dynamics of hospital infection in order to effectively prevent and control it.METHODS From 12306 cases of inpatients who had investigated retrospectively,706 cases were with nosocomial infection monitored from Jan to Dec 2006.RESULTS The hospital infection rate was 5.74% in 2006.The hematological disease and,cancer patients and the elderly,were the high-risk population.The main infection site was the respiratory tract.Fungi accounted for 21.35%,the top was the Candida albicans.CONCLUSIONS To strengthen the training of medical staff,to monitor the key departments of hematology and cancer and respiratory diseases and to attend the disease surveillance and rationally use of antibiotics are the effective measures to reduce hospital-acquired infections.

Objective To study the findings of measles associated with pneumonia and to improve the diagnostic level of this disease.Methods The chest X-ray of 280 cases with pneumonia among 310 children with measles from January 2005 to August 2006 were collected and analyzed retrospectively.Results The most common findings of chest X-ray examinations included blurred and increased lung markings(in all cases),pulmonary air-sacs luminance increase and emphysema-like change in 206 cases(73.6%),spotted shadows and patches in the bilateral middle and lower field in 91 cases(32.5%),enlarged and blurred pulmonary hila in 115 cases(41.1%),interstitial appearance in 98 cases(35%),serious pneumonia and ARDS in 4 cases(1.4%).Conclusion It is valuable in the diagnosis and differential diagnosis of measles accompanying pneumonia in children to analyze the chest X-ray combining with clinical situation.

Based on the characteristics of medicinal chemistry and the training objectives of experimental teaching,to explore the teaching reform of comprehensive experiments in medicinal chemistry.This paper discussed the time of biginning comprehensive experiments,the choice of experimental article,the demands on teachers in comprehensive experimental teaching,and attention points of teaching management.

OBJECTIVE: To evaluate the capability and limitation of magnetic resonance image(MRI)for Lumbar zygapophyseal joint cartilage through comparing pig lumbar zygapophyseal joint cartilage acquired from multiple MRI sequences of a 1.5 Tesla MR and gross specimens. METHODS: Six fresh lumbar spines from adult pigs were sagittaly scanned by Siemens 1.5 Tesla MR. The scan sequences included fast spin echo T1-weighted imaging (FSE T1WI), fast spin echo T2-weighted imaging (FSE T2 weighted T2WI), fat saturation proton density-weighted imaging (FS PDWI), 3-dimensional fast low angle shot imaging (3D-FLASH), and water excitation 3-dimensional fast low angle shot imaging (WE 3D-FLASH). Each scan sequence acquired images from the same layer. The signal-noise ratio (SNR) for articular cartilage, contrast-noise ratio (CNR) for cartilage versus bone cortex, cartilage versus bone marrow, and cartilage versus saline were calculated. Right after the scanning, the lumbar spines were snap-frozen, incised sagittally along the midline lumbar zypapophyseal joints, and photographed to compare the gross specimens with corresponding MRIs. The thickness of sagittal midline center of 6 pairs of lumbar(L₃/L₄) zypapophyseal joint cartilage was measured by vernier caliper. The thickness of the back ventral articular cartilage was added and then compared with corresponding MR images. RESULTS: 3D-FLASH (FA 20°) and WE 3D-FLASH (FA 20°) sequences had significant advantages compared with other sequences in imaging lumbar zypapophyseal joint cartilage, and were mostly close to the real thickness.(1) Comparison of the 4 flip angle (FA 10°, FA 20°, FA 30°, and FA 40°) 3D-FLASH sequences:The highest cartilage SNR and best CNR of cartilage versus bone cortex were both found in the 3D-FLASH(FA 20°) sequence, which was significantly different from the other three 3D-FLASH sequences.The satisfactory CNR of cartilage versus bone marrow, cartilage versus saline were found more in the 3D-FLASH(FA 20°) sequence. (2) Comparison of the 4 flip angle(FA 10°, FA 20°, FA 30°, and FA 40°) WE 3D-FLASH sequences: the highest cartilage SNR,best CNR of cartilage versus bone cortex,and best CNR of cartilage versus bone marrow were found in the WE 3D-FLASH (FA 20°) sequence, which was significantly different from the other three 3D-FLASH sequences. The CNR of cartilage versus saline was found more satisfactory in the WE 3D-FLASH (FA 20°) sequence. (3) The highest cartilage SNR and best CNR of cartilage versus bone cortex were both found in the 3D-FLASH (FA 20°) sequence, which was significantly different from those in the PDWI, FSE T1WI,and FSE T2WI sequences (P<0.05), but with no significance (P>0.05) in the WE 3D-FLASH (FA 20°) sequence. The highest CNR of cartilage versus bone marrow was seen in WE 3D-FLASH (FA 20°) sequence. It was statistically significant compared with that in FS PDWI,FSE T1WI, and T2WI sequences respectively, but the difference was not significant compared with 3D-FLASH (FA 20°) sequence (P>0.05). Both the FS PDWI and T2WI sequences displayed ideal CNR of cartilage versus saline, with no significant difference (P>0.05). The lower SNR of cartilage versus saline was shown in 3D-FLASH (FA 20°) and WE 3D-FLASH (FA 20°) sequence, and the difference was not significant (P>0.05). However, they were significantly different compared with FS PDWI and T2WI sequences (P<0.05). (4) WE 3D-FLASH (FA 20°) and 3D-FLASH (FA 20°) sequences were relatively better than the FS PDWI when comparing the thickness of articular cartilage, which was significantly different from the FS PDWI sequence (P<0.05). CONCLUSION The 3D-FLASH sequence and derived WE 3D-FLASH sequence have better definition of cartilage images and are mostly close to the real thickness, which possibly are the optimal scanning sequences for lumbar zypapophyseal joint articular cartilage MR imaging.
Animals ; Cartilage, Articular ; anatomy & histology ; Imaging, Three-Dimensional ; Lumbar Vertebrae ; anatomy & histology ; Magnetic Resonance Imaging ; methods ; Swine ; Zygapophyseal Joint ; anatomy & histology

Objective To study the phenotype and function of CD4 +CD25 + Treg cells in the peripheral blood of patients with systemic sclerosis (SSc) and their relationship with fibrosis.Methods The proportion of Foxp3, CD127, CTLA-4 and CD69 on CD4+CD25+ Treg cells in peripheral blood were detect by flow cytometry;the levels of TGF-[1 and IL-10 in serum were detect by enzyme-linked immunosorbent assay (ELISA) in patients with SSc.The correlation between Treg cells and the score of chest HRCT, MRSS, and disease activity was analyzed.T test and Pearson correlation analysis were used for statistical analysis.Results ① Compare to the control group, the proportion of CD4+CD25+ Treg cells in peripheral blood of SSc patients was increased significantly(12.9±2.4 vs 14.9±2.2, t=2.63, P=-0.012), and the expression of CD69+, CTLA-4+ on CD4+CD25+ Treg cells was decreased significantly (P＜0.01).② Compare to the control group, the proportion of CD4+CD25+Foxp3 + cells and CD4+CD25+CDI27-cells in peripheral blood of SSc patients was increased significantly (respectively, 3.3±0.7 vs 5.0±0.7, 5.1±1.6 vs 7.6±2.0, t=7.03, 4.195;P＜0.01), but no correlation between them was detected.③ The level of TGF-β1 in the serum of the SSc patients was lower than that of the control group(86±29 vs 133±29 ng/ml, t=-5.026, P=0.000).However, IL-10 had no significant difference between the two groups.④ The proportion of CD4+CD25 +Foxp3 + cells and CD4~D25 +CD127-cells in peripheral blood of SSc patients was positively correlated with the scores of chest HRCT (respectively, r=-0.541, P=0.02;r=0.486, P=0.041), and no correlation was observed with ESR, CRP.In addition, CD4+CD25+Foxp3+ cells were associated with MRSS.Conclusion The proportion of CD4+CD25+ Treg cells in the peripheral blood of SSc patients is increased, but they alters the immune function.The different phenotypes of Treg cells of CD4+CD25+Foxp3+ cells and CD4+CD25+CD127-cells in peripheral blood of SSc patients are increased significantly, which changes along with skin and lung fibrosis.The associated cytokine TGF-β1 is reduced, and IL-10 is not significantly changed.

Objective To analyze the HLA-A ,B and DRB1 alleles high-resolution polymorphism in Chongqing Han population . Methods The PCR-SSOP and PCR-SBT methods were applied for the HLA high-resolution genotyping of 2 067 unrelated healthy donors in the registry of Chongqing branch of Chinese National Marrow Donor Program (CMDP) .The allele frequencies of HLA-A , B and DRB1 were estimated by the direct counting method and the Hardy-Weinberg equilibrium inspection was performed by using the Arlequin software 3 .1 .Results 168 high-resolution alleles were detected out ,in which 42 alleles of A*11 :01 ,A*24:02 ,A*02:07 ,A*02 :01 and A*33:03 at the HLA-A locus were observed with the frequencies greater than 0 .05 ;81 alleles were detected at HLA-B locus ,including B*46 :01 ,B*40:01 ,B*58 :01 ,B*13 :01 and B*15 :02 with the frequencies greater than 0 .05 ;45 al-leles of DRB1*09:01 ,DRB1*15 :01 ,DRB1*12 :02 ,DRB1*08 :03 and DRB1*11 :01 at the HLA-DR locus were observed with the frequencies greater than 0 .05 .Conclusion The data of the HLA-A ,B and DRB1allelic frequencies at high-resolution level in Chongqing Han population are obtained ,which provides the reliable reference data for the studies of anthropology ,forensic medi-cine ,transplantation matching and disease association .

Objective To investigate antimicrobial resistance among gram-positive cocci in China in 2006.Methods From Jun 2006 to Dec 2006,674 consecutive and non-repetitive gram-positive bacteria were collected from 7 teaching hospitals.The minimal inhibitory concentration(MICs)of antibacterial agents were determined by agar dilution method.Results The prevalence of penicillin.resistant(ease)and pemcllhn. intermediate S.pneumoniae(PISP)among 100 isolates was l%and 19%,respectively.Teicoplanin and vancomycin were the most active agents against S.pneumoniae.97% and 98% S.pneumoniae isolates were susceptible to levofloxacin and moxifloxacin,respectively.The susceptibility of amoxicillin/clavulanic acid, ceftriaxone and chloramphenicol are 96%,87% and 73%,respectively.The susceptible rates of penicillin. susceptible S.pneumoniae(PSSP)to cefprozil and cefaclor were 62% and 55.7%,respectively.All the PISP and PRSP isolates were resistance to the two antibiotics.The susceptibility to macrolides,trimethoprim/ sulfamethoxazole and tetracycline was lower than 35%.The prevalence of methicillin-resistant Staphylococcus aureus(MRSA)and methicillin.resistant coagulase-negative Staphylococci(MRSCON)was 48%(33%-84%)and 81%(69%-94%),respectively.The susceptible rates of MRSA to trimethoprim/ sulfamethoxazole,chloramphenicol,rifampin,and the other antibiotics in this study were 72%,66%and 45%,respectively.The susceptible rate of MRSA to marcrolides,aminoglycosides,tetracyclines and quinolones were not more than 18%.56%(30%-86%)of E.faecalis and 80%(50%.100%)of E.faecium were highly resistant to gentamicin.The susceptibility of E.faecalis to all the antibiotics except chloramphenicol and tetracycline were higher than E.faecium.All isolates of S.aureus,CoNS and E.faecalis tested were susceptible to vacomycin and teicoplanin.There were two vacomycin.resistant E.faecium strains isolated from Hangzhou.Conclusions Antimicrobial resistance patterns of gram.positive cocci differed in different regions.The resistance of gram-positive cocci to the antibiotics in this study this year was a little higher than the data of the year of 2005.Teicoplanin and vancomycin remained very high activity to gram-positive cocci.

Objective To investigate antimicrobial resistance among gram-positive cocci in China in 2009. Methods From June to December 2009, 1169 consecutive and non-repetitive gram-positive cocci were collected from 12 teaching hospitals at 9 cities. The minimal inhibitory concentration (MIC) of antibacterial agents was determined by agar dilution method. Results The prevalences of methicillinresistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative Staphylococci (MRCoNS) were 45.3% (211/466) and 89. 5% (214/239), respectively. The isolation rate of MRSA was 33. 3%-68. 1% from different samples. All Staphylococci isolates were susceptible to vacomycin, teicoplanin and linezolid. Five point five percent (7/128) E. faecium strains were resistant to vacomycin. All E.faecalis strains were susceptible to vacomycin. About 99. 1% (108/109) of E. faecalis and E. faecium were susceptible to linezoild. The prevalence of penicillin-intermediate Streptococcus pneumoniae (PISP) was 21.6% (48/222). Only 1 (0. 5%, 1/222) Streptococcus pneumoniae strain was resistant to penicillin.Teicoplanin, vancomycin, linezolid and tigecycline were the most active agents against Streptococcus pneumoniae (susceptible rate 100% ). Conclusions The high prevalence of methicillin-resistance is among Staphylococcus strains. Different samples show a different MRSA prevalence. Teicoplanin, vancomycin and linezolid show very high activity to Staphylococci,E. faecalis, E. faecium and Streptococcus pneumoniae.