Tumor dormancy is a phase of occurrence and progress of cancer.In recent years,with the further study of circulating tumor cells,disseminated tumor cells and cancer stem cells,it is found that they are closely related to the tumor dormancy.Moreover,tumor microenvironment is another important factor in tumor dormancy.

The paper introduces the system structure of the knowledge discovery system of digital library of medical university,including knowledge service interaction layer,knowledge discovery processing layer,data mining & transmission layer and basic data resource layer,and discusses the knowledge service mode of the digital library of medical university supported by knowledge discovery,namely,the knowledge service mode based on embedded system,document database and collaborative mode.

The effects of Trichostatin A (TSA) on histone deacetylase 8 (HDAC8) expression, proliferation and cell cycle arrest in T-lymphoblastic leukemia cell line Molt-4 cells in vitro were investigated. The effect of TSA on the growth of Molt-4 cells was studied by MTT assay. Flow cytometry was used to examine the cell cycle. The expression of HDAC8 was detected by using immunocytochemistry and Western blot. The results showed that proliferation of Molt-4 cells was inhibited in TSA-treated group in a time- and dose-dependent manner. The IC50 of TSA exposures for 24 h and 36 h were 254.3236 and 199.257 microg/L respectively. The cell cycle analysis revealed that Molt-4 was mostly in G0/G1 phase, and after treatment with TSA from 50 to 400 microg/L for 24 h, the percents of G0/G1 cells were decreased and cells were arrested in G2/M phase. Treatment of TSA for 24 h could significantly inhibit the expression of HDAC8 protein in Molt-4 cells (P<0.01). It was concluded that TSA could decrease the expression of HDAC8 in Molt-4 cells, which contributed to the inhibition of proliferation and induction of cell cycle arrest in Molt-4 cells.

Objective To observe the effects of Huoxue Xifeng Decoction (HXD) on endothelin-1 (ET-1) and nitric oxide (NO) levels in the blood and brain tissue of focal cerebral infarction (FCI) rats. Methods Forty male Wistar rats were randomized into 5 groups:sham-operation, FCI model and HXD-high, HXD-middle, HXD-low dose groups, with 8 rats in each group. The model of FCI rats was established by photo-chemistry method, and intragastric administration were continue 7 days before operation. HXD-high, HXD-middle, HXD-low dose groups were given HXD 20, 10, 5 g/(kg?d) respectively, and other groups were given the same volume distilled water. Blood and brain tissue samples were gotten 24 h after operation, and radio-immunity method was used to measure ET-1 level, spectrophotometric method was used to measure NO level. Results Compared with FCI model group, HXD decreased ET-1 level in plasma and brain tissue after infarction, and decreased NO level in the brain tissue, increased NO level in serum (P

Objective To explore drug abuse prevention measures by surveying the changes before and after methadone maintenance treatment(MMT). Methods The baseline survey data were obtained from patients who participated in the MTT program for the first time at Wuhan First Health Clinic of Mental Health Center Affiliated to Tongji Medical College between March 2006 and December 2013,and the general conditions of these patients were analyzed. Results There were 1 186 drug abusers, with a male and low education(junior high school and below)dominance. After the initiation of the MMT program,the number of addicted people was highest in 2008,and then gradually decreased after 2009.MMT program achieved obvious social benefits.The proportion of injectable drug use alone was decreased and the rates of oral drug use and snorting were increased over time.Fixed salary and temporary salary were obviously increased in drug abusers after 2009. Daily drug cost was decreased over time. The proportion of community/ media propaganda through which people got to know MMT remained low. Conclusion The community support and educational propaganda should be strengthened at the same time when MMT is carried out.

The CD19-targeted chimeric antigen receptor (CAR) T-cell treatment has achieved clinical success in treating B-cell lineage hematopoietic malignancies. This accomplishment involved the precise and efficient elimination of tumor cells by tumor-associated an-tigen-redirected immune cells. As a result, the reinitiation of several CAR T-cell (CART) based clinical trials in solid tumors was promot-ed. However, developing a feasible and efficient CAR T based therapeutic modality for solid tumors is urgently needed given the differ-ential properties between liquid and solid tumors. In this review, we discuss the advances in the management of cytotherapeutic mo-dality for solid tumors. The aspects considered include toxicity management, target selection, and primer or conditioning treatment.

OBJECTIVE:To establish a method for the identification and content determination of leguminosae in Qizhu bishi granules. METHODS:Thin layer chromatography (TLC) was used to identify the leguminosae. HPLC-ELSD was used to deter-mine the content of astragaloside A in leguminosae. The column was Diamonsil C18 with the mobile phase of acetonitrile-water(32∶68,V/V)at the flow rate of 1.0 ml/min,the column temperature was 40 ℃. The sample size was 20 μl. The drift tube temperature was 108 ℃ with the nitrogen gas flow rate of 2.8 ml/min. RESULTS:The TLC features of leguminosae were obvious with clear spots and good specificity. Astragaloside A had a good linear relationship in the range of 0.402 4-4.02 μg(r=0.997 0). RSDs of pre-cision,stability and reproducibility tests were≤0.8%. The average recovery was 99.00%(RSD=1.29%,n=9). CONCLUSIONS:The method is simple,accurate and reliable and can be used for the quality control of Qizhu bishi granules.

Objective To observe the clinical effect of phloroglucinol combined with diazepam in prolonging the latency of primipara and the influence on the safety of mother and infant after medication .Methods A total of 180 cases with a naturally occurring post-partum latency≥8 h and no progress of patients in Weifang Tranditional Chinese Hospital from June 2015 to June 2016 in this study were divided into control group and observation group with 90 cases in each group.The observation group were treated with phloroglucinol combined with diazepam,the control group were treated with diazepam.Two groups appeared uterine inertia when the discretion of intravenous infusion of 0.5% oxytocin injection.Two groups were observed to 16h, still no progression of labor related to obstetric intervention.Observed of cervix big open situation,cervical opened time, cervical edema rate, cervical laceration rate, the rate of vaginal delivery, two hours postpartum hemorrhage, Apgar scores of newborns and the adverse reaction of medicine. Results The duration from drug administration to full dilation of cervix in obseve group was significantly shorter than the control group(P<0.05),the rate of cervical edema in the observation group was significantly lower than the control group(P<0.05), but no statistically difference in the cervical laceration.The the rate of vaginal delivery in observation group decreased significantly when compared with the control group ( P<0.05 ) , two hours postpartum hemorrhage and the Apgar scores of newborns had no significant differcences.Conclusion Puerpera latency prolonged application of phloroglucinol combined with diazepam can significantly speed up the cervix to open the time , and reduce cervical edema and improve vaginal delivery rate,and cervical laceration,two hours postpartum hemorrhage and neonatal complications had no effect.

Objective:To discuss the quality control method of injection pump and analyze the factors that affect the quality control of injection pump.Methods: Flow error and blocking alarm error were tested by the INFUTEST 2000E dual-channel infusion pump analyzer according to the national calibration specifications.Results: The syringe specifications, injection speed and injection solvent could influence the test for accuracy of injection pump. The lower injection speed set, the bigger flow test error was, while the smaller the blocking alarm test error was. On the other hand, the higher the injection speed set, the smaller the flow test error was and the bigger the blocking alarm test error was. Besides, the higher qualified rate of flow test was, the lower the qualified rate of blocking alarm test was.Conclusion: The quality control of injection pump should abide by the principle of controllable risk, and should not pursuit too much accuracy.

Objective To investigate the mechanisms of mesenchymal stromal cells (MSCs) in promoting the transplanted islets neovascularization.Methods We performed co-transplantation of allogeneic islet and MSCs into non-obese diabetic (NOD) mice and the lymphocyte chimeric rate was detected by flow cytometry.The MSCs migration,transformation and secretion of cytokines were detected by immunofluorescence.Results In mice of co-combination transplantation of islet with MSCs,donor lymphocytic chimerism in graft was increased.BM-MSCs can migrate to transplanted islet,differentiate into vascular smooth muscle cells,and also secrete vascular endothelial growth factor (VEGF).Conclusions MSCs can migrate to transplanted islet and promote neovascularization.Also,it enhanced allograft immune tolerance of islet grafts via increasing donor lymphocytic chimerism.