1.Clinical analysis of 495 elderly patients with eyelid neoplasms
Jibing YU ; Ling WANG ; Jingfang HAO ; Hongkui ZHANG ; Lu YANG
Chinese Journal of Geriatrics 2017;36(9):1002-1004
2.A clinical investigation on factors influencing the prognosis of primary liver cancer patients after hepatectomy
Xiqing JI ; Chaolong LI ; Jincheng YANG ; Hongkui YANG ; Xingguo LIU ; Menglong WANG ; Zhiqi LIN
Chinese Journal of General Surgery 1993;0(02):-
Objective To explore factors influencing the prognosis of hepatocellular carcinoma (HCC) patients after hepatectomy. Methods From May 1994 to January 1998, 189 patients who underwent hepatectomy for HCC were enrolled for reviewing their clinical characteristics, treatment and prognosis. Twenty-two parameters contributing to long-term survival rate (SR) and disease-free SR were analysed. Results The 3,5-year cumulative SR of the whole group was 63%,45%, respectively. The 3,5-year SR and disease-free SR in the curative resection (CR) group ( n =162) were 67%,47% and 45%,26% respectively. Results showed that the way by which a tumor was found, tumor size, portal tumor thrombi, satellite nodule, TNM stage, cirrhosis type, recurrent and treatment, blood transfusion, differentiation grade,and CR were risk factors by individual variable analysis( P =0.0000~0.0034); A multivariable analysis showed that CR, tumor size, tumor finding mode and reoperation were significant factors associating with prognosis( P =0.0000~0.0024). Blood transfusion and type of cirrhosis were closely correlated with tumor-free survival ( P =0.0001). Conclusions Curative resection, tumor size, reoperation for recurrence were important factors for recurrence by multivariate analysis. The severity of concomittant liver cirrhosis and perioperative blood transfusion were closely correlated with postoperative tumor free survival.
3.Efficient derivation of embryonic stem cells from NOD-scid Il2rg (-/-) mice.
Kang LIU ; Riguo FANG ; Haibo LI ; Weifeng YANG ; Zhenchuan MIAO ; Jinhua WEN ; Hongkui DENG
Protein & Cell 2015;6(12):916-918
4.Correction to: Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg mice.
Yaqin DU ; Ting WANG ; Jun XU ; Chaoran ZHAO ; Haibo LI ; Yao FU ; Yaxing XU ; Liangfu XIE ; Jingru ZHAO ; Weifeng YANG ; Ming YIN ; Jinhua WEN ; Hongkui DENG
Protein & Cell 2019;10(2):154-155
In the original publication Fig. 1D and supplementary material is incorrect. The correct figure and supplementary material is provided in this correction.
5.Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg mice.
Yaqin DU ; Ting WANG ; Jun XU ; Chaoran ZHAO ; Haibo LI ; Yao FU ; Yaxing XU ; Liangfu XIE ; Jingru ZHAO ; Weifeng YANG ; Ming YIN ; Jinhua WEN ; Hongkui DENG
Protein & Cell 2019;10(1):31-42
Recently we have established a new culture condition enabling the derivation of extended pluripotent stem (EPS) cells, which, compared to conventional pluripotent stem cells, possess superior developmental potential and germline competence. However, it remains unclear whether this condition permits derivation of EPS cells from mouse strains that are refractory or non-permissive to pluripotent cell establishment. Here, we show that EPS cells can be robustly generated from non-permissive NOD-scid Il2rg mice through de novo derivation from blastocysts. Furthermore, these cells can also be efficiently generated by chemical reprogramming from embryonic NOD-scid Il2rg fibroblasts. NOD-scid Il2rg EPS cells can be expanded for more than 20 passages with genomic stability and can be genetically modified through gene targeting. Notably, these cells contribute to both embryonic and extraembryonic lineages in vivo. More importantly, they can produce chimeras and integrate into the E13.5 genital ridge. Our study demonstrates the feasibility of generating EPS cells from refractory mouse strains, which could potentially be a general strategy for deriving mouse pluripotent cells. The generation of NOD-scid Il2rg EPS cell lines permits sophisticated genetic modification in NOD-scid Il2rg mice, which may greatly advance the optimization of humanized mouse models for biomedical applications.