The article summarized the practice teaching experience of traumatology: strengthening the learning education of medical ethics and the knowledge of psychology,understanding the specificity and complexity in traumatology,following the rule of evidence-based medicine and establishing clinic thoughts,strengthening the training of basic skill,basic knowledge and basic theory and helping the interns transit to clinical clerkship.

Objective To investigate the expression of E-cadherin (E-cad), CD44v6 and VEGF-C in esophageal carcinoma and investigate their relationship with lymph node metastasis. Methods The expression of E-cad, CD44v6 and VEGF-C was detected with immunohistochemical EnVision method in 56cases of esophageal carcinoma and 12 cases of normal esophageal mucosa. Results Among 56 cases of esophageal carcinoma, the positive expression rates of E-cad in esophageal carcinoma was 55.4 %(31/56),significantly lower than that in normal esophageal mucosa 100.0 %(12/12) (P ＜0.05). The positive expression rates of CD44v6 and VEGF-C were 71.4 % (40/56) and 62.5 % (35/56), significantly higher than that in normal esophageal mucosa (P ＜0.05). The positive rates of E-cad was 31.3 % (10/32) in the group of positive lymph node metastasis, significantly lower than that in the group of positive non-lymph-node metastasis 87.5 % (21/24) (P＜0.05). The positive rates of CD44v6 and VEGF-C were 84.4 % (27/32) and 75.0 % (24/32) in the group of positive lymph node metastasis ,significantly higher than that in the group of positive non-lymph-node metastasis 54.2 % (13/24) and 45.8 %(11/24) (P＜0.05). E-cad was negatively correlated with lymph node metastasis; CD44v6 and VEGF-C were positively correlated with lymph node metastasis (P ＜0.05). Conclusion E-cad, CD44v6 and VEGF-C play important roles in the process of soakage and metastasis of esophageal carcinoma. The combined detection of E-cad, CD44v6 and VEGF-C can help to prodict biological behaviors and prognosis of esophageal carcinoma.

Objective; To evaluate the effects of gingival shade guide in visual shade-matching in vitro models. Methods; 42 tabs of Shofu NCC shade guide were adopted for color measuring. Each tab was fixed in the middle of Gingiva-Indicator, Guray(1,2,3,4) respectively and located in a black box. The measurements were made using the Shadepilot instrument. The color parameters ( CIE1976L * a * b * ) of cervical, body, incisal area of each tab were recorded. The color difference A E value in the same position of each tab was calculated between non-gingival shade guide group and Gumyl ,2,3,4 groups with Shadepilot software. Results; The AE value in the same position of 42 tabs between non-gingival shade guide group and 4 gingival shade guide groups were; cervical (2.93±0.79)NBS,body(0.51±0.27)NBS and incisal(0.61 ±0.29)NBS. In the cervical, body and incisal area, there was no statistically difference among Gumyl ,2,3,4 groups on affecting shade of tabs(P >0. 05). Conclusion; The gingival color has an effect on the cervical shade of tabs, and it can be recognized by human eyes, while gingival color has little effect on the shade of body and incisal area.

Objective To investigate the expression of EphrinB2 and microvessel density(MVD) in colorectal carcinoma and to analyze the relationship between the expression of EphrinB2 and the biological characteristics of colorectal carcinoma. Methods EphrinB2 and MVD of 63 colorectal carcinoma diagnosed by pathology were examined by immunohistochemistry, and 25 specimens from normal colorectal tissue were examined as controls. Results The expression of EphrinB2 protein was 82.0 % (51/63) in colorectal carcinoma and 32.0 % (8/25) in the normal colorectal tissues, the difference was significant (P <0.05). Meanwhile, MVD was significantly higher in EphrinB2 positive group (39.39±8.97) than that in negative group (33.00 ±10.19) (P<0.05). The expression of EphrinB2 protein had significant positive correlation with differentiation grade, infiltration, lymph node metastases and clinical stage (P <0.05), and had no relation to age, sex, tumor size and location(P >0.05). Conclusion The EphrinB2 protein expression increase may be involved in development of colorectal carcinoma and play important roles in the tumorigenesis and progression of colorectal carcinoma.

Objective To investigate the difference of retinal nerve fiber layer thickness(RNFL)in opti-cal coherence tomography(OCT)under low and moderate signal strength. Methods Four hundred eyes of people aged 46~75 with clear fundus image,no obvious fundus diseases,and satisfactory optical coherence tomography were classified according to their ages and signal strength. The peripapillary and 4 quadrants of RNFL were detect-ed with OCT. Results In satisfactory OCT images ,the signal strength that reached 4/10 and 7/10 was 1.5% and 27% respectively. Under low and moderate signal strength ,the maximum thickness of RNFL was at the superior and inferior,and the minimum thickness of RNFL was at the nasal and temporal. There were no significant differ-ences in RNFL thickness under low or moderate signal strength. Conclusion RNFL results are reliable under low and moderate signal strength.

Objective To find up a suitable beginning scan time so that to assure the image quantity during CTA of brain.Methods 30volunteers were checked by heart colour sonography first,then CTA at earlier stage was done,when the density in basilar artery high up to120 HU,that time was regarded the time as beginning scan time,the relative curve between beginning scan time and ejection fraction(EF) was drew,according to this relative curve,34 patients were examined by CTA,the displaying situation of vessel was evaluated comparatively with DSA in part of patients.Results According to this method,the successful rate of CTA in patient’s group was 100%,the trunk of brain vessel and its 1~3 level branch were showed in 100%,the 4~5 level branch of middle cerebal artery showed in 94.1%(32/34).Conclusion According to EF of heart to determine the beginning scan time of CTA is practical,that the image quantity of CTA can be assured.

Objective To understand the result coincidence of 4 hematology analyzers (LH780 ,LH750 ,XN-1000 ,and XS-1000i) .Methods 20 samples of routine blood test were selected and detected by different hematology analyzers whose within-run precision and between-run precision all met the requirements of WS/T 406 standard .LH750 was chose to be the reference instru-ment ,and its detection results were standard values .The relative deviations and coincidence rates of WBC ,RBC ,Hb ,HCT , PLT ,MCV ,MCH and MCHC of the other 3 hematology analyzers were calculated .The WBC and PLT coincidence results of 82 cases of low-WBC samples ,86 cases of low-PLT samples ,and 35 cases of platelet aggregation samples were investigated .Results The coincidence rates of all items were more than 90% between LH780 and LH750 .For XN-1000/XS-1000i ,the coincidence rate was 80% in HCT ,and which were less than 80% in MCV and MCHC ,and more than 85% in other items .After readjusting the calibration factors of HCT of XN-1000 and XS-1000i ,the coincidence rates of HCT ,MCV and MCHC were all increased to more than 85% .The coincidence rates of WBC and PLT were less than 70% in low-WBC samples (WBC<1 .0 × 109/L) and low-PLT samples (PLT<30 × 109/L) .The results of WBC and PLT could not reach the relative deviation standards in 35 platelet aggrega-tion samples .Conclusion The comparative tests should be carried out among different hematology analyzers in one laboratory .

Objective To evaluate an agarose gel electrophoresis for quantitative determination of Lipoprotein(a) cholesterol and its clinical application.Methods Quantification of LP(a)-C was performed by a new agarose gel electrophoresis method that allows the separation of LP(a) by Hellen REP system and the determination of LP(a)-C by enzymatic staining of cholesterol,The results of electrophoresis method were compared with the ones of INA and ITA. The specimens of 135 health men were assayed by electrophoresis for the reference range.Results The inter and intra CV of electrophoresis method at low, middle and high LP(a) concentration specimens were 4.7%～5.3% and 5.8%～6.4%. The linearity was 0.058～1.55 mmol/L. Interference with bilirubin (

Surface transcutaneous electric nerve stimulation(TENS) was applied to treat the spasiticity resulting from spinal cord injury(SCI) in the acupoints of the subjects with SCI in order to oberve thetranscient and long-term effect of the treatment Fand its mechanism will be discussed in this paper. The results show that: 1 )more significant effect in relieving the spasiticiy was got by stimulating in high frequencyfor 30minutes,while less effect in low frequency(2Hz) ; 2)the effect in high frequency was partially ostracized by naloxone; 3)the effect in high frequency lasted for a long time i 4)compared with lumbar and sacralstimulation,the acupoint stimulation lasted for the longest time. This study also gets a hypothesis that highfrequency TENS in acupoints improves dynorphin in CSF to release and significantly relieve the spasiticityby kappa opioid receptor which decreases the excitment of anterior motor neuron.

Objective To explore the diagnosis, treatment and pathogenesis of solitary rectal ulcer syndrome (SRUS) in patients with spinal cord injury (SCI). Methods 260 patients with SCI accepted rectal diseases survey, anal examination and anus straight endoscopic. Those who had rectal ulcer also performed biopsy, and patients who had been diagnosed as SRUS were evaluated by the index of International bowel function basic SCI data set, then further accepted non-operative or surgical treatment, and regular reexamination with anal straight colonoscopy for clinical evaluation after discharge. Results and Conclusion Bowel dysfunction, constipation, and anal rectum prolapsed, improper uses of glycerine enema were important causes of SRUS after SCI. Non-operative therapy as well as strengthening defecate management could be an effective treatment for SRUS after SCI.