NaCl.Glycerol and trehalose were the most prominent compatible solutes in C.krusei.The glycerol production was enhanced with the osmotica concentration increasing and NaCl,KCl were much effective than manntiol.The glycerol concentration was increased 74%,63%,57% and the intracellular glycerol was 3.1,2.4,1.8 fold to the control respectively when 0.6mol/L NaCl,KCl and mannitol existed.During the early stage of fermentation,the trehalose all decreased compared with the control.However,the trehalose content was higher than the control in the late stage of fermentation when 0.6mol/L KCl and mannitol existed.The trehalose content was 1.6 and 1.4 fold to that of control respectively.

Objective: To understand the overall situation and problems of the human resources in community medical institutions in Heilongjiang, put forward relevant measurement and suggestions, hoping to provide references for departments of the government designing human resources policies in community medical institutions in Heilongjiang. Methods: Through consulting some related literature information, to recognize the related policies and development situation of community health human resources in Heilongjiang and other places in China. Using Excell2003 to set up the database and analyze. Results:The community health service system has been established in Heilongjiang, but it lacks of human resources planning; the community health human resource configuration is not reasonable; and the existing community health persons’ training demand is higher. Conclusion: Community health service institutions in Heilongjiang should strengthen the government leadership, designed relevant policies, adjust and optimize the structure, speed up the training of the current staff and attract the talents.

Objective To investigate the clinical efficacy of trimetazidine in treatment of ischemic cardiomy-opathy heart failure and its influence on cardiac function and heart rate variability .Methods 160 cases of patients with ischemic cardiomyopathy heart failure were randomly divided into the two groups with 80 cases in each group .The
two groups were given conventional treatment of anti heart failure ,the observation group used trimetazidine on basis of that,clinical effect and the influence on the index of heart rate variability and cardiac function of two groups were ob -served.Results The total effective rate of the observation group was 91.25%,significantly higher than 73.75% of the control group (χ2 =9.57,P<0.05);LVEDD and LVESD of the observation group after treatment were (49.88 ± 4.60)mm and (37.10 ±3.15)mm,significantly reduced than before treatment (t=7.44,7.83,all P<0.05) and the control group (t=6.60,6.85,all P<0.05); while the LVEF was (48.04 ±4.20)%,increased significantly than before treatment (t=6.51,P<0.05) and the control group (t=7.11,P<0.05);SDNN,SDANN,rMSSD and PNN50 of the observation group after treatment were (124.72 ±7.02)ms,(111.20 ±5.89)ms,(46.74 ±4.22)ms and (17.72 ±2.08)%,increased significantly than before treatment (t=7.43,7.83,7.06,8.17,all P<0.05) and the control group (t=6.66,6.90,6.15,7.21,all P<0.05).Conclusion Trimetazidine has an exact effect in treat-ment of ischemic cardiomyopathy heart failure ,which can improve heart function and heart rate variability which has an important value in clinical application .

Objective To observe the association between the polymorphism of PINK1 gene exon 4 T313M and Parkinson's disease ( PD) in Xinjiang Uygurs.Methods The genetic DNA was extracted from 175 Uygur patients with PD (PD group) and 163 Uygur healthy controls (control group).The PINK1 gene exon 4 T313M was amplified by PCR.The genotype and allele were determined by restriction enzymes enzyme technology, and then validated by sequencing analysis.Results The frequency of PINK1 gene T313M T/T genotype in PD group was 2.9%, which of C/C genotype was 97.1%;the frequency of PINK1 gene T313M T allele in PD group was 2.9%, which of C allele was 97.1%.The frequency of PINK1 gene T313M T/T genotype in control group was 1.2%, which of C/C genotype was 98.8%;the frequency of PINK1 gene T313M T allele in control group was 1.2%, which of C allele was 98.8%. The difference of the polymorphism of PINK1 gene T313M between the two groups had no statistical significance ( P>0.05).Conclusion The polymorphism of PINK1 gene exon 4 T313M has nothing to do with the genetic susceptibility of Xinjiang Uighur PD patients.

Objective To study the efficacy and safety of Fasudil in treatment of elderly diastolic heart fail -ure related pulmonary hypertension ,and provide evidence for clinical treatment .Methods 70 cases of elderly dias-tolic heart failure related pulmonary hypertension were divided into the study group and control group of 35 cases in each group according to random number table methods ,the two groups were given beta blockers ,angiotensin converting enzyme inhibitors and other drugs for treatment ,the study group was given fasudil on basis of that ,6min walking dis-tance,pulmonary artery systolic pressure ,arterial partial pressure of oxygen ( PaO2) of two groups before and after treatment as well as the changes of blood pressure and heart rate of the study group before and after treatment were compared.Results Before treatment,6min walking distance,pulmonary artery systolic pressure and PaO2 of the two groups were (290.3 ±1.3)m,(83.3 ±0.9)mmHg,(66.3 ±1.4)mmHg and (289.2 ±1.1)m,(82.9 ±0.8)mm-Hg,(65.9 ±1.3)mmHg showed no statistical significance (t=2.392,4.927,5.132,P=0.073,0.069,0.063),after treatment,6min walking distance ,pulmonary artery systolic pressure and PaO 2 of the study group were (410.4 ±2.3) m,(60.1 ±1.1) mmHg,(89.3 ±0.6) mmHg significantly better than those of the control group ,with statistically significant difference(t=8.918,9.142,11.319,P =0.024,0.019,0.017);the systolic blood pressure,diastolic pressure and heart rate of the study group before and after treatment were (132.8 ±2.1)mmHg,(84.3 ±1.1)mm-Hg,(69.2 ±1.4) times/min and (133.2 ±1.9) mmHg,(83.9 ±0.9) mmHg,(68.9 ±1.3) times/min showed no statistical significance(t=4.193,4.018,3.987,P=0.061,0.078,0.068).Conclusion Fasudil has a better effect in treatment of elderly diastolic heart failure related pulmonary hypertension ,which can significantly reduce the pulmo-nary artery pressure ,and has a little effect on the heart ,with high safety .

Small cell lung cancer (SCLC) is a highly malignant tumor, which is very sensitive to first-line chemotherapy, but easy to recurrence and metastasis early.So it always has poor prognosis.Second-line therapy for SCLC develops slowly.Topotecan is the only drug approved by US Food and Drug Administration as a second-line chemoradiotherapy.Numerous studies on molecular targeted therapy and immunotherapy are being carried out, but most of them have no or little benefit.Rovalpituzumab tesirine (Rova-T) targeted antibody-drug conjugate (ADC) which is published at the 2016 American Society of Clinical Oncology Annual Meeting shows a good anti-tumor activity, which seems to bring a new dawn of molecular targeted therapy.

Objective To investigate the intervention effect of Qingxin-Ⅱ Recipe on myocardial cell apoptosis and its fibrosis in viral myocarditis mice and its mechanism.Methods A total of 120 Balb/c mice were randomly divided into the model group (n=50),the treatment group (n=50) and the control group (n =20).Mice in the model and treatment groups were given intraperitoneal injection of CVB3 to establish the experimental model of viral myocarditis.The control and model groups were treated with normal saline and water by intragastric administration respectively and the treatment group was given Qingxin-Ⅱ Recipe.The expression level of nuclear factor-κB (NF-κB),tumor necrosis factor (TNF-α),interleukin-1β(IL-1β),collagen type Ⅰ-A1 (COL1-A1),collagen type Ⅲ-A1 (COL3-A1),matrix metalloproteinase-9 (MMP-9),tissue inhibitor of metallproteinase-1 (TIMP-1),myocardial interstitial collagen volume fraction (CVF) and apoptosis rate (Rapo) were assessed after treatment.Results The levels of NF-κB,TNF-α,IL-1 in cardiomyocytes,serum levels of COL1-A1,MMP-9,TIMP-1,the mRNA expression levels of COL1-A1,COL3-A1,MMP-9,TIMP-1 in cardiomyocytes,CVF and Rapo were lower in the treatment and control groups than in the model group (F=21.541,12.146,32.583,4.212,6.863,4.868,9.089,8.662,7.307,6.646,4.324,41.237,respectively,all P＜0.05).No significant differences in the levels of NF-κB,TNF-α,IL-1 of cardiomyocytes,in serum levels of COL1-A1,TIMP-1 and in mRNA expression levels of COL1-A1,COL3-A1,MMP-9,TIMP-1 of cardiomyocytes between the control group and the treatment group (all P＞0.05) were found,but serum MMP-9 level was higher in the treatment group than in the control group (P =0.033).Conclusions Qingxin-Ⅱ Recipe can effectively reduce inflammatory cytokines levels,protect myocardial cells from inflammatory damages and inhibit myocardial fibrosis in viral myocarditis.

Objective To investigate the significance of detecting the level of CRP,TNF-α,IL-6 and IGF-1 in the patients with type 2 diabetes mellitus(T2DM).Methods Sixty patients with T2DM in the Pingliang Municipal People′s Hospital from February 2013 to March 2015 were selected as the observation group And contemporaneous 60 individuals undergoing healthy physical exami-nation were selected as the control group.The levels of FBG,2 hBG,FINS,HbA1c,CRP,TNF-α,IL-6 and IGF-1 were detected in these two groups.The correlation between CRP,TNF-α,IL-6 and IGF-1 with FBG,FINS and HbA1c were analyzed.Results The levels of FBG,2 hBG and HbA1c in the observation group were significantly higher than those in the control group,the difference was statistically significant (P<0.05).The FINS level in the observation group was significantly lower than that in the control group,the difference has statistically significant (P<0.05).The levels of CRP,TNF-α,IL-6 and IGF-1 in the observation group were (8.46±2.45)mg/L,(18.56±4.23)ng/L,(13.53±3.24)pg/L and (125.33±23.14)ng/L respectively,which were signifi-cantly higher than (3.23±1.02)mg/L,(11.25±3.01)ng/L,(7.24±1.78)pg/L and (98.45±18.56)ng/L in the control group, the difference was statistically significant(P<0.05).CRP,TNF-αand IL-6 were positively correlated with FBG,FINS and HbA1c;IGF-1 was negatively correlated with FBG and HbA1c.Conclusion Detecting the CRP,TNF-α,IL-6 and IGF-1 levels can provide a basis for the diagnosis of T2DM and thinking for its treatment.

Hospital medical ethics is the basis of survival,and an important part of management in modern hospitals.Through the implementation of humanistic services,regulated medical behaviors,expeditious communication channels,and satisfying service for patients with all efforts,can modern hospitals establish a good image for themselves and gain fine reputation from patients.

OBJECTIVE:To discuss the interactions between tetracyclines(oxytetracycline,methacycline,tetracydine,and doxycycline)and human serum albumin(HSA).METHODS:The binding constants and the number of biding sites of tetracyclines with human serum albumin(HSA)at various temperatures were studied by using UV-visible absorption spectrum and spectrofluorimetry,and the enthalpy change(?H)entropy change(?S)were computed.According to the Frster nonradiative energy transfer theory,the critical distances R0 between donators and acceptors were derived.RESULTS:The thermodynamic parameters were as follows:?H0;R0:oxytetracycline:(1.82 nm),and methacycline(2.31 nm),tetracydine(2.98 nm),doxycycline(2.26 nm).CONCLUSION:Tetracyclines can all cause HSA's fluorescence quenching.The quenching mechanism is static quenching.The main acting force between them is electrostatic attraction.