NaCl.Glycerol and trehalose were the most prominent compatible solutes in C.krusei.The glycerol production was enhanced with the osmotica concentration increasing and NaCl,KCl were much effective than manntiol.The glycerol concentration was increased 74%,63%,57% and the intracellular glycerol was 3.1,2.4,1.8 fold to the control respectively when 0.6mol/L NaCl,KCl and mannitol existed.During the early stage of fermentation,the trehalose all decreased compared with the control.However,the trehalose content was higher than the control in the late stage of fermentation when 0.6mol/L KCl and mannitol existed.The trehalose content was 1.6 and 1.4 fold to that of control respectively.

Objective: To understand the overall situation and problems of the human resources in community medical institutions in Heilongjiang, put forward relevant measurement and suggestions, hoping to provide references for departments of the government designing human resources policies in community medical institutions in Heilongjiang. Methods: Through consulting some related literature information, to recognize the related policies and development situation of community health human resources in Heilongjiang and other places in China. Using Excell2003 to set up the database and analyze. Results:The community health service system has been established in Heilongjiang, but it lacks of human resources planning; the community health human resource configuration is not reasonable; and the existing community health persons’ training demand is higher. Conclusion: Community health service institutions in Heilongjiang should strengthen the government leadership, designed relevant policies, adjust and optimize the structure, speed up the training of the current staff and attract the talents.

Objective To observe the clinical effect of psychological intervention combined with alendronate and low molecular heparin calcium on the treatment of senile osteoporotic fracture. Methods From February 2014 to March 2017, clinical data of 82 elderly patients with osteoporotic fracture in the first people's hospital in Jiande were retrospectively analyzed. All the patients were randomly divided into the control group and the experimental group with 41 cases in each group. The control group patients were given alendronate sodium and low molecular heparin calcium; At this basis, the experimental group were received psychological nursing intervention. The effect in the two groups was compared. Results After treatment, bone mineral density (BMD) index and pain scores in the two groups were significantly improved. BMD and pain scores in the experimental group were significantly better than those in the control group (P<0.05). The total effective rate was 95.12% (39/41) and the complication rate was 17.07% (7/41) in the experimental group, while in the control group were respectively 70.73% (29/41), 36.59% (15/41), the differences were statistically significant (P<0.05). Conclusion Psychological intervention combined with alendronate sodium and low molecular heparin on the treatment of senile osteoporotic fracture has significant effect, can effectively improve the clinical symptoms of patients, reduce the incidence of complications, with clinical application value.

Objective To investigate the clinical efficacy of trimetazidine in treatment of ischemic cardiomy-opathy heart failure and its influence on cardiac function and heart rate variability .Methods 160 cases of patients with ischemic cardiomyopathy heart failure were randomly divided into the two groups with 80 cases in each group .The
two groups were given conventional treatment of anti heart failure ,the observation group used trimetazidine on basis of that,clinical effect and the influence on the index of heart rate variability and cardiac function of two groups were ob -served.Results The total effective rate of the observation group was 91.25%,significantly higher than 73.75% of the control group (χ2 =9.57,P<0.05);LVEDD and LVESD of the observation group after treatment were (49.88 ± 4.60)mm and (37.10 ±3.15)mm,significantly reduced than before treatment (t=7.44,7.83,all P<0.05) and the control group (t=6.60,6.85,all P<0.05); while the LVEF was (48.04 ±4.20)%,increased significantly than before treatment (t=6.51,P<0.05) and the control group (t=7.11,P<0.05);SDNN,SDANN,rMSSD and PNN50 of the observation group after treatment were (124.72 ±7.02)ms,(111.20 ±5.89)ms,(46.74 ±4.22)ms and (17.72 ±2.08)%,increased significantly than before treatment (t=7.43,7.83,7.06,8.17,all P<0.05) and the control group (t=6.66,6.90,6.15,7.21,all P<0.05).Conclusion Trimetazidine has an exact effect in treat-ment of ischemic cardiomyopathy heart failure ,which can improve heart function and heart rate variability which has an important value in clinical application .

Objective To observe the clinical effect and investigate the mechanisms of Yikun Oral Liquid in treating climacteric syndrome due to yin deficiency of liver and kidney. Methods One hundred and three cases of climacteric syndrome of yin deficiency of liver and kidney were observed, including 70 cases of treatment group (treated with Yikun Oral Liquid) and 33 cases of control group (treated with Tibolone tablets). Quantitative studies were performed on the clinical symptoms (using Kupperman marking standard), levels of estradiol (E2), progesterone (P), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and endometrial thickness respectively before and after treatment. Results Clinical symptoms were improved obviously in two groups after treatment (P

Objective To establish the quality standard for Lithospermum anti-inflammatory tablets.Methods Houttuynia,diffusa and grass coral in the prescription were qualitative identified by Thin Layer Chromatography(TLC) method.Results TLC spot features clear and specific.Conclusion The established standard can be used for quality control of Lithospermum anti-inflammatory tablets.

Objective To investigate the intervention effect of Qingxin-Ⅱ Recipe on myocardial cell apoptosis and its fibrosis in viral myocarditis mice and its mechanism.Methods A total of 120 Balb/c mice were randomly divided into the model group (n=50),the treatment group (n=50) and the control group (n =20).Mice in the model and treatment groups were given intraperitoneal injection of CVB3 to establish the experimental model of viral myocarditis.The control and model groups were treated with normal saline and water by intragastric administration respectively and the treatment group was given Qingxin-Ⅱ Recipe.The expression level of nuclear factor-κB (NF-κB),tumor necrosis factor (TNF-α),interleukin-1β(IL-1β),collagen type Ⅰ-A1 (COL1-A1),collagen type Ⅲ-A1 (COL3-A1),matrix metalloproteinase-9 (MMP-9),tissue inhibitor of metallproteinase-1 (TIMP-1),myocardial interstitial collagen volume fraction (CVF) and apoptosis rate (Rapo) were assessed after treatment.Results The levels of NF-κB,TNF-α,IL-1 in cardiomyocytes,serum levels of COL1-A1,MMP-9,TIMP-1,the mRNA expression levels of COL1-A1,COL3-A1,MMP-9,TIMP-1 in cardiomyocytes,CVF and Rapo were lower in the treatment and control groups than in the model group (F=21.541,12.146,32.583,4.212,6.863,4.868,9.089,8.662,7.307,6.646,4.324,41.237,respectively,all P＜0.05).No significant differences in the levels of NF-κB,TNF-α,IL-1 of cardiomyocytes,in serum levels of COL1-A1,TIMP-1 and in mRNA expression levels of COL1-A1,COL3-A1,MMP-9,TIMP-1 of cardiomyocytes between the control group and the treatment group (all P＞0.05) were found,but serum MMP-9 level was higher in the treatment group than in the control group (P =0.033).Conclusions Qingxin-Ⅱ Recipe can effectively reduce inflammatory cytokines levels,protect myocardial cells from inflammatory damages and inhibit myocardial fibrosis in viral myocarditis.

The hospital culture constrction should stick to a human-orientedconcept ,and aim to provide humanitarian brand services.Hospitals should create a favorable hospitalizing condition,and foster a standard treatment,which embodies the feathers of the present time while bearing its own characteristics .It should focus on the in-depth cultural constrction and try to carry out cultural management of the hospital,the leaders of the hospital play a critical role in the cultural constrction

The paper introduces microcosmic control model for medical morals construction.The function of the model includes explanation,operation and evaluation.The author holds that the key to the morals construction is scientific management,and the decision maker should have the social responsibility and the love-patient consciousness.

Hospital medical ethics is the basis of survival,and an important part of management in modern hospitals.Through the implementation of humanistic services,regulated medical behaviors,expeditious communication channels,and satisfying service for patients with all efforts,can modern hospitals establish a good image for themselves and gain fine reputation from patients.