Menarche and menopause are two important physiological periods that denote the beginning and the end of normal reproductive life.Studies have suggested that ages at menarche and menopause are important biological markers,and are also predictors of several chronic diseases.We published one manuscript in J Clin Endocrinol Metab titled Associations between age at menarche and menopause with cardiovascular disease,diabetes,and osteoporosis in Chinese women,which suggested that ages at menarche and menopause are not associated with diabetes,delayed menarche and menopause are associated with decreased risk of cardiovascular disease risk and early menopause with high risk of osteoporosis.

Actins ; metabolism ; Calcium-Binding Proteins ; metabolism ; Calmodulin-Binding Proteins ; metabolism ; Desmin ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Hysterectomy ; Leiomyoma, Epithelioid ; metabolism ; pathology ; Leiomyomatosis ; metabolism ; pathology ; surgery ; Leiomyosarcoma ; pathology ; Microfilament Proteins ; metabolism ; Middle Aged ; Receptors, Estrogen ; metabolism ; Receptors, Progesterone ; metabolism ; Rhabdoid Tumor ; metabolism ; pathology ; surgery ; Sarcoma, Endometrial Stromal ; metabolism ; pathology ; Uterine Neoplasms ; metabolism ; pathology ; surgery ; Vascular Neoplasms ; metabolism ; pathology ; surgery ; Veins ; pathology ; Vimentin ; metabolism

Objective To Analyze the distribution characteristics of Hyperthyroidism's syndrome and the relationship between symptoms and thyroid hormones. Methods The TCM syndromes of a total of 78 patients with hyperthyroidism were differentiated. The relationship between syndrome and general condition of patients (gender and age, etc.) were analyzed;correlation between symptoms and thyroid hormones were also studied. Results Of all 78 patients, patients with yin deficiency and hyperactive yang accounted for 43.6%; patients with internal disturbance of phlegm-fire accounted for 30.8 %; and patients with hypteracitivity of heart-fire and liver-fire accounted for 25.6%. The severity of all the symptoms showed positive correlation with hyroid hormones (the value of r is positive, P＜0.05); and no linear relationship with TSH (P＞0.05).Conclusion The syndrome of yin deficiency and hyperactive yangI was the most common syndrome type. Thyroid hormones had direct proportion with the severity of syndrome.

Objective To evaluate the primary application of drug eluting stent for treatment of vertebral stenosis. Methods One vertebral original stenosis was treated by CYPHER TM stent and the relative papers were reviewed. Results A male of 53 years old with paroxysmal vertigo and left hemiplegy revealed nothing abnormal by magnetic resonance imaging (Diffusion Weighted Imaging). But the DSC found his right vertebral origin 4.6 mm long stenosis (more than 90%), the remote artery was 2.6 mm in diameter, and angiography in the right vertebral origin showed right post inferior cerebellar artery (PICA) insufficiently filled and no development of basilar artery. When angiography in the left vertebral, there was no fill in the right PICA. The restenosis rate after vertebral origin stent posting was as high as 43.3%, but drug eluting stent can prevent restenosis. After preparation, a 2.75 mm?13 mm balloon-expandable Cypher stent was posted in on 2004-06-07. Angiography showed the stenosis disappeared, right PICA sufficiently filled, and basilar artery appeared. Antiplatelet agents were used afterwards. There was no attack of vertigo and hemiplegy in the followed-up month. Conclusions The success of using drug eluting stent in verebral artery offers a new choice on preventing ischemia stroke, the long time followed-up is necessary.

Objective To compare the test bolus technique with the bolus tracking technique for dual-energy CT pulmonary angiography in patients suspected of pulmonary embolism (PE).Methods A total of 60 patients were randomized into 2 groups:bolus tracking technique group (group B,n =30) and test bolus technique group (group T,n =30).A standard dual-energy CT pulmonary angiography (CTPA)was performed after injection of 60 ml contrast medium followed by 35ml saline chaser at a speed of 4 mL/s.Attenuation profiles of different vascular segments (the subclavian vein,superior vena cava,left atrium,pulmonary trunk,S1 and S10 pulmonary artery) and enhancement value of lung parenchyma were measured to evaluate the timing techniques.The overall image quality of CTPA and pulmonary perfusion were analyzed by two radiologists.SPSS 19.0,Student t test,ANOVA test,Chi-Square test,Mann-Whitney U test and Kappa test were used for the statistic analysis.Results The attenuation of the subclavian vein [(1042 ± 639),(2200 ± 724) HU,F =43.196,P =0.001],superior vena cava [(529 ± 237),(904 ± 329)HU,F=25.654,P=0.001],pulmonary trunk [(325 ±112),(383±69)HU,F=5.94,P=0.018],S1 pulmonary artery [(320 ± 118),(385 ±73) HU,F =6.549,P =0.013],S10 pulmonary artery [(307 ± 78),(360 ± 82) HU,F =6.658,P =0.012] in group B were lower than those in group T,while the attenuation of the left atrium in group B was higher than that in group T [(270 ± 53),(219 ± 57)HU,F=12.823,P =0.001].The percentage of pulmonary arteries exceeding optimal attenuation (＞ 250 HU) in groups B was lower than that in group T [n =21 (70.0％),n =29 (96.7％),x2 =5.88,P ＜ 0.05].The enhancement value of pulmonary parenchyma in group B was lower than that in group T (the right lung (29±8)vs(34±8) HU,t=-2.401,P=0.020; the left lung (30±7)vs(36±9)HU,t=-2.493,P=0.016; total (30 ±7)vs(35 ±8) HU,t =-2.495,P =0.016).The image quality of CTPA in group B was poorer than that in group T (U =619.5,P ＜ 0.05,Kappa =0.708),while the artifacts of lung perfusion image in group B was less than that in group T (U =162.5,P ＜ 0.05,Kappa =0.796).Conclusion The test bolus technique has a better image quality of CT pulmonary angiography and a higher lung perfusion blood volume compared with the bolus tracking technique by using 60 ml contrast medium and 35ml saline.

Objective To investigate the antitumour effects of transfected gut-enriched Krüppellike factor(GKLF) on human gastric carcinoma cell line SGC-7901 in vitro and in vivo. Methods The expression of GKLF mRNA and protein in human gastric carcinoma cell line SGC-7901 were detected before and after transfection by real-time fluorescence quantitative PCR and Western blot,respectively. Proliferation and invasion in SGC-7901 were measured respectively by MTT assay, flow cytometry, colony formation assay and cell invasion assay after transfected with GKLF. The growth of xenograft was observed, the microvessel density(MVD) of xenograft tissue was determined by immunohistochemistry. Results The GKLF mRNA and protein in SGC-7901 were overexpressed after transfected with GKLF(P＜0.05). The proliferative speed of SGC7901-pcDNA3.1-GKLF group was markedly lower than that of SGC-7901 and SGC7901-pcDNA3.1 groups (P＜0.05). Transfected with GKLF caused part of the G0/G1 arrest, decreased clone formation rate and the invasion ability (P＜0.05). The growth speed of xenograft in SGC7901-pcDNA3.1-GKLF group was lower, the weight and MVD of xenograft tissue in SGC7901-pcDNA3. 1-GKLF group were less (P＜ 0. 05).Conclusion Transfected with GKLF maysuppress proliferation and invasion in human gastric carcinoma cell line SGC-7901, inhibit the growth and the angiogenesis of xenograft in nude mice.

Objective To explore the MRI characterisitics of primary dilated cardiomyopathy and isolated left ventricular non-compaction.Methods The patients who were diagnosed as primary dilated cardiomyopathy (n=18) and isolated left ventricular non-compaction (n=10) were enrolled,and the MRI was performed.The thickness of non-compaction myocardium (NC),compaction myocardium (C) in end-diastole,the feature of movement of myocardium,the number of non-compaction segment,the fraction shortening of non-compaction and the distribution were compared.Results Totally 823 segments were analyzed in primary dilated cardiomyopathy,in which 124 segments were non-compaction myocardium;397 segments were analyzed in isolated left ventricular non-compaction,in which 115 segments were non-compaction myocardium.The NC,NC/C,NC/(NC+C),and the fraction shortening of the isolated left ventricular non-compaction patients were higher than those of primary dilated cardiomyopathy patients (all P＜0.05).The features of distribution showed that the apical segment was mostly involved,and the basal segment was less involved or hardly involved.The anterior and lateral segments were more involved in the free wall,the septal was less involved.Conclusion The MRI characteristics of primary dilated cardiomyopathy and isolated left ventricular non-compaction are different,especially in the distribution,non compacted segments,NC and NC/C,which is important for diagnosis and differential diagnosis of the two diseases.

Objective To suvey the clinical application of antibacterials and drug resistance of antibacterials in the hospital in 2013.Methods To make statistical analysis on the frequency of drug use and drug resistance rate in 2013.Results Piperacillin wasn′t used in 2013 in the hospital.No hemolytic streptococcus was detected with drug resistance.Drug resistance rate of Esche-richia coli to piperacillin was 33.22%.Conclusion Bacterial resistance might be related to the using frequency of drugs and the types of antibacterials used in a region.

Objective To investigate the effect of propofol on autophagy in SD rat heart during myocar-dial ischemia-reperfusion (I/R) injury. Methods Twenty-one male SD rats were randomly divided into three groups as follows (n = 7): the sham operation group, in which rats underwent sham operation without tightening of the coronary artery sutures; the myocardial ischemia-reperfusion group , in which rats were induced by occlud-ing the left anterior descending coronary artery for 30 min , followed by 120 min reperfusion and 0.9% NaCl in-fusion at 3 mL/(kg·h) at 10 min before occluding the left anterior descending coronary artery; the myocardial ischemia- reperfusion- propofol group, in which rats underwent I/R and propofol infusion at 6 mg/(kg·h) at 10 min before occluding the left anterior descending coronary artery. Before tightening of the coronary artery, at 30 min post-tightening of the coronary artery and at 120 min post-reperfusion, HR、 LVSP and ± dp/dtmax of rats were recordedin each group. Atter 120 min post-reperfusion, the serum concentrations of cTnT was measured. The in-jured cardiac tissue was collected to investigate the ultrastructure change under the TEM and to determine the levels of mTOR and p-mTOR. Results No signifcant differences in HR, LVSP and ± dp/dtmax before tighten-ing of the coronary artery. But, at 30 min post- tightening of the coronary artery, compared with groupⅠ, the HR, LVSP and ±dp/dtmax were significantly decresed in groupⅡ and Ⅲ(P < 0.05). Then, at 2 h post-reper-fusion, compared with groupⅠ, the HR, LVSP, ±dp/dtmax and the level of p-mTOR were significantly de-creased, but the serum concentration of cTnT was significantly increased in groupⅡ(P < 0.05); but, compared with groupⅡ, the HR, LVSP, ± dp/dtmax and the level of p-mTOR were significantly increased, the serum concentration of cTnT and the level of mTOR were significantly decreased in group Ⅲ(P < 0.05). Conclusions These data suggest that propofol could heighten the level of p-mTOR, and attenuate the expression of mTOR dur-ing the myocardial ischemia-reperfusion injury in SD rats.

Objective To elucidate the role of the Wnt/-catenin signaling pathway in regulating the phenotypic transformation of aortic valvular myofibroblasts to osteoblast-like cells.Methods Cultured primary valvular myofibroblastes isolated from porcine aortic valve leaflets were treated with oxidized low-density lipoprotein (ox-LDL) for different lengths of time:24 h,48 h and 72 h.The Wnt signaling pathway inhibitor Dickkopf-1 (DDK-1) was co-incubated with ox-LDL for 72 h.After cells harvest,the expression of myofibroblastic or osteoblast-like phenotype related markers,a-smooth muscle actin (α-SMA),bone morphogenetic protein 2 (BMP2),alkaline phosphatase (ALP) and corebinding factora-1 (Cbfα 1),was detected by Western blotting.The expression and sub cellular localization of β3-catenin was assessed by immunocytochemistry.Changes of the Wnt/β-catenin signaling pathway and the transformation of aortic valvular myofibroblast to osteoblast-like cells were monitored.Results BMP2,ALP and Cbfa 1 protein expression was not or barely detectable in the control group.However,after ox-LDL treatment,the expression of α SMA,BMP2,ALP and Cbfa 1 increased significantly (each P＜0.01) in a time-dependent manner (each P＜0.05).Besides,ox-LDL was also able to up-regulate the protein expression of β-catenin in a time-dependent manner (P＜0.05) and promoted its nuclear translocation.After DKK-1 treatment,the protein expression of β3 catenin and osteogenesis related markers was down regulated (P＜0.05).Conclusions The Wnt/β-catenin signaling pathway may play a crucial role in regulating the transformation of aortic valvular myofibroblasts to an osteoblast like phenotype.