Objective To investigate effects of Tanshinone Ⅱ A (Tan Ⅱ) on proliferation and apoptosis of myeloblastic leukemia cell lines.Methods NB4,K562 and THP-1 cells were incubated with TanⅡA for 24,48 and 72 hours.Ddaunorubicin was used as a positive control.Cell proliferation was monitored by MTT assay.Cell apoptosis and cell cycle were determined by Annexin Ⅴ-FITC/PI flow cytometry.Expression of Caspase-3 was quantified by spectrophotometry.Results After incubation with various leukemia cells for 24,48 and 72 hours,Tan Ⅱ inhibited proliferation of NB4 cells with IC50 of 24.11,9.60 and 7.28 μmol/L,inhibited K562 cells with IC50 of 31.75,11.88 and 6.81 μmol/L and inhibited THP-1 cells with IC50 of 111.10,32.82 and 11.82,respectively.After treatment with Tan Ⅱ for 48 hours,cell apoptosis,the number of G1 phase cells and the expression of Caspase-3 in all three leukemia cell lines were increased significantly comparing with the blank control group (P ＜ 0.05).Conclusions Tan Ⅱ A has proliferation inhibitory effect on myeloblastic leukemia cell lines by the order of effect NB4＞K562＞THP-1.Tan ⅡA displays anti-leukemia activity possibly through arresting leukemia cells in G1 phase and inducing apoptosis by increasing Caspase-3 expression.

The numbers of application and granted funds for NSFC were analyzed statistically in Capital Medical University Affiliated Beijing Anzhen Hospital from 2009 to 2012.This paper retrospectively analyzed the development recurrent features of scientific research and put forward some suggestions.Meanwhile,it revealed the prospects of scientific research in our hospital.

Objective To observe the clinical effect of treating adenomyosis uterine artery embolization (UAE)after interventional therapy for adenomyosis with Huayuxiaojie decoction. Methods A total of 40 patients with adenomyosis were randomly recruited into a control group and a treatment group. 20 patients in the control group were not given any medical treatment after interventional operation; while 20 patients in the treatment group were given the decoction of Huayuxiaojie after intervention. Changes of menstrual blood volume, menalgia degree, CA125 and uterine volume of all patients were investigated after the treatment. Results Patients in the treatment group showed significantly better results than the control group in the improvement of menstrual blood volume, dysmenorrhea, CA 125, and uterine volume (P＜0.05) .Conclusion Huayuxiaojie decoction was effective in relieving menalgia, adjusting menorrhagia, soothing bellyache,reducing fever of syndromes after interventional operation of adenomyosis.

Pylorus obstruction caused by foreign bodies in the gastric anrum and granulomatous inflammation is rarely seen.The clinical symptoms of this disease are unspecific.Combination of X-ray radiography,computed tomography and gastroscopy could make definite diagnosis.Differential diagnosis between pylorus obstruction and peptic ulcer,gastric cancer and duodenal obstruction should be done before operation.On April 13,2012,a patient with pylorus obstruction caused by foreign bodies in the gastric anrum and granulomatous inflammation was treated at the Second Hospital of Jiaxing,the imaging characteristics of the disease were summarized to provide referrence for the diagnosis and treatment of this disease.

Objective:To examine whether the use of moderate hypothermic circulatory arrest in a pig model provides comparable vital organ protection outcomes to the use of deep hypothermic circulatory arrest.Methods:Thirteen pigs were randomly assigned to 30 minutes of hypothermic circulatory arrest without cerebral perfusion at 15℃(n=5), 25℃(n=5) and a control group(n=3). The changes in standard laboratory tests and capacity for protection against apoptosis in different vital organs were monitored with different temperatures of hypothermic circulatory arrest management in pig model to determine which temperature was optimal for hypothermic circulatory arrest.Results:There were no significant differences in the capacity for protection against apoptosis in vital organs between 2 groups( P>0.05, respectively). Compared with the moderate hypothermic circulatory arrest group, the deep hypothermic circulatory arrest group had no significant advantages in terms of the biologic parameters of any other organs( P>0.05). Conclusion:Compared with deep hypothermic circulatory arrest, moderate hypothermic circulatory arrest is a moderate technique that has similar advantages with regard to the levels of biomarkers of injury and capacity for protection against apoptosis in vital organs.

Objective To study the expression of thymic stromal lymphopoietin(TSLP) and the activation of DCs in OVA-induced murine asthma model, and investigate the effects and underlying mecha-nisms of TSLP on lung inflammation. Methods Thirty BALB/c mice were randomly divided into control group, OVA group and TSLP neutralizing antibody treated group. The asthma model was evaluated by airway responsiveness and histological analysis of lung tissues ; The levels of TSLP mRNA in lungs were determined by quantitative real-time PCR; The expression of TSLP in lungs were determined by immunohistochemistry and Western blot; The expression of CD40, CD80, CD86 in BALF was detected by FACS. Results Both the histological analysis of lung tissues and the airway responsiveness were all consistent with the characteris-tic of murine asthma model. The expression of TSLP and TSLP mRNA in the OVA group was significantly in-creased compared with blank group. The expression of CD40, CD80, CD86 in BALF from OVA group was increased significantly compared with the control group. Furthermore, treating mice with TSLP neutralizing antibody reduced the expression of CD40, CD80, CD86 on dendritic cells, and IL-4, IL-5, IL-13 in the OVA group. Conclusion Our study indicate that TSLP was highly expressed in the bronchial epithelia of murine asthma model, via upregulation of CD40, CD80, CD86, induce DCs to active CD4~+ T cells and pro-duce type 2 responses, so that aggravating the lung inflammation of asthma. Blocking TSLP is capable of in-hibiting the production of Th2 cytokines, thus presents a promising strategy for the treatment of asthma.

Objective To evaluate systematically whether or not the treatment of herbs invigorating spleen and removing dampness,blood stasis and toxin with chemotherapy is better than chemotherapy alone for advanced colorectal cancer.Methods The randomized controlled trails (RCT) involving combined treatment of herbs with chemotherapy,published from January 2000 to October 2015,were searched in CBM,CNKI,Wanfang Data,VIP,PubMed,Embase databases.Stata 14.0 software was used to analyze the data.Results 19 RCT containing 941 patients were included in this reta-analysis.Compared with chemotherapy alone,the combined treatment of Chinese herbal medicine and chemotherapy was obviously better in 1-year survival rate (RR =1.28,95 ％ CI 1.14-1.45),2-year survival rate (RR =1.52,95 ％ CI 1.05-2.18),3-year survival rate (RR =2.76,95 ％ CI 1.56-4.88),objective response (RR =1.11,95 ％ CI 1.04-1.19),Karnofsky score (RR =1.46,95 ％ CI 1.27-1.68) and traditional chinese medicine (TCM) symptom score (RR =1.58,95 ％ CI 1.33-1.75).The adverse effect rate was statistically reduced in the combined treatment group:leukopenia (RR =0.59,95 ％ CI 0.40-0.8),nausea and vomiting (RR =0.68,95 ％ CI 0.59-0.79),diarrhea (RR =0.67,95 ％ CI 0.53-0.85),neurotoxicity (RR =0.79,95 ％ CI 0.65-0.96).Conclusion Compared with chemotherapy alone,the treatment of herbs invigorating spleen and removing dampness,blood stasis and toxin combined with chemotherapy for advanced colorectal cancer can significantly increase survival rate and objective response rate,improve the quality of life,and meanwhile decrease the adverse effect rate.

OBJECTIVE:To determine the content of phenolic acids from Anemone altica,and optimize its extraction technolo-gy. METHODS:HPLC was used to determine the contents of mono-ferulyl tartaric acid and ferulic acid from A. altica;using the total contents of 2 index components as index,volume fraction of extraction solvent,extraction solvent volume,extraction times and extraction time as factors,orthogonal test was used to optimize extraction technology,and verification test was conducted. RE-SULTS:The contents of mono-ferulyl tartaric acid and ferulic acid were 0.059%,0.0025%,respectively;the optimal extraction technology was as follow as 30% ethanol 600 mL added to 20 g medicinal material,extracted twice,90 min every time. In verifi-cation test,the average contents of 2 components in extract were 0.2971%(RSD=3.64%,n=3),0.0041%(RSD=5.11%,n=3). CONCLUSIONS:A method for contents determination of mono-ferulyl tartaric acid and ferulic acid from A. altica is estab-lished;optimized extraction technology is stable and feasible.

A total of 59 untreated asthmatics and 47 healthy control subjects were recruited from Qianfoshan Hospital of Shandong Province from May 2011 to April 2012.Compared with healthy control subjects,the levels of IL-25 in induced sputum and eosinophils,IgE,interleukin-4 (IL-4) and interleukin25 (IL-25) in serum samples of asthmatics were significantly higher while the level of interferon-gamma (IFN-γ) were much lower.However,after inhaled glucocorticoid treatment,the levels of eosinophils,IL-4 and IL-25 decreased and IFN-γ significantly increased,while the level of IgE showed no significant changes.It was also found the expression of IL-25 was markedly positively correlated with the levels of eosinophils and IL-4 in serum and markedly negatively correlated with the levels of IFN-γ and had no relatio.

Objective:To investigate the current situation of ICU nursing humanistic care delivery chain in the tertiary hospitals of Heilongjiang Province, and to analyze the related factors affecting the ICU nursing humanistic care delivery chain, and to provide basis for formulating management measures to improve the ICU nursing humanistic care delivery chain.Methods:A total of 531 nurses from ICUs in five tertiary hospitals in Heilongjiang Province were surveyed using the ICU Nursing Humanities Care Assessment Scale to analyze the overall score and influencing factors of the scale.Results:The overall score of the Nursing Humanities Care Atmosphere Scale of Heilongjiang Provincial third-level hospital was (168. 31± 3.72) points. Different genders, ages, education, hospital attention to nursing, humanistic care training, working hours, leadership had a statistically significant difference in the scores of the scales of their own interest ( t or F values were -2.634, 9.718, 4.857, 7.847, 3.094, 5.709, 8.856, P < 0. 05 or 0.01). The influencing factors of ICU nursing humanistic care delivery chain were academic level, hospital attention to nursing, humanistic care training, working time, and the level of concern of leaders to themselves. The degree of dependent variable could be explained as 36.8%. Conclusion:The ICU care humanities care delivery chain atmosphere in the tertiary hospitals in Heilongjiang Province is at a medium level and is affected by many factors.