[Objective] To observe the influence of different kinds of biological pesticide on the fruiting rate and yield of Amomum villosum Lour. (AVL) . [Methods] Medicinal plant field experiment method was used to observe the influence of biological pesticide A (highly effective immuno-biologic bactericide, 75.76mg?m-2 for one time) and its 1000-fold, 800-fold and 500-fold diluent on the fruiting rate and yield of AVL. Meanwhile, the influence of biological pesticide B (edible oligosaccharide, 30.30mg?m-2 for one time) and biological pesticide C (deguelin emulsion, 1.89mg?2m-2 for one time) was also observed. [Results] The three biological pesticides, as well as the diluents of biological pesticide A increased the fruiting rate and yield of AVL, the influence of the moderate-concentration biological pesticide A being the greatest. [Conclusion] Proper application of biological pesticide is one of the important ways to raise the yield of AVL.