Caveolae are cholesterol-rich pits on the surface of cell membrane,and participate in various physiological processes.Caveolin-1 is an important membrane protein constituting caveolae.It exerts biological effects through binding a variety of signaling proteins.In the pathophysiological processes of cerebrovascular disease,caveolin-1 participates in maintaining the integrity of blood-brain barrier and stability of atherosclerotic plaques,and has regulating the release of inflammatory mediators and neuroprotection.This article reviews the associated advances in research on the correlation between caveolin-1 and cerebrovascular disease in order to provide a new idea for clinical therapy.

Objective] Discuss the“five seasonal organs”theory of The Inner Canon of Huangdi, especially the seasonal issues of the spleen and preliminarily speculate the formation period. [Methods]By means of literature and historiography methods, information before The Inner Canon of Huangdi would be gathered, especially“The Book of Rites·on Order”,“Guanzi·Seasons”,“Annals by Lv·Twelve Ji”and so on. On this basis, we can discuss the different perspectives of “five seasonal organs”theory and the seasonal issues of the spleen of The Inner Canon of Huangdi, and preliminarily speculate the theory’s origin, evolution, and formation. [Results]“Six periods connected to six internal organs”theory is more concerned with the meaning of anatomy, although it is early view of the“seasonal organs”theory, but six or twelve has its specific cultural origins;“Eight wind connected to eight internal organs”theory is affected by Zhou Yi;“four periods connected to four internal organs”theory couldn’t explain attribution of the“soil”and its role, it belongs to the early stages of Qi argumentation of the“seasonal organs”theory after the introduction of the Five Elements Theory;“five periods connected to five internal organs”theory reflected the tight integration with the theory of five elements and balanced attachment features; the theory which has the most theoretical and clinical meaning is“four periods connected to five internal organs”theory. [Conclusion] The different perspectives of“five seasonal organs”theory and the seasonal issues of the spleen of The Inner Canon of Huangdi are results of a number of philosophical thoughts absorbed by different doctors, but through analysis of the relevant literature, the core ideas can be briefly summarized, it can be generalized that the spleen is located in the center, connected at any time, behalf of governance after each season ’s eighteen days.

Eisenmenger syndrome (ES) was considered as a surgical contraindication in the past time. Only a few patients got lung transplantations or heart-lung transplantations due to lack of donors. Most of the patients had to choose conservative treatment to prolong their survival time. Recently, some clinical trials indicated targeted therapies in pulmonary arterial hypertension (PAH) were effective for the treatment of ES. This article reviewed the recent advances in this area.

To evaluate security of influenza virus vaccines by analysis of adverse reaction to influenza virus vaccine,especially the relationship between Guillain Barr? syndrome and influenza vaccines.

We investigated whether platelet-activating factor (PAF) mediates endotoxin-induced changes of lipoperoxidation, lysosomal enzyme release and increased extravascular lung water content in dogs using a specific PAF receptor antagonist, SRI 63-441. Endotoxin infusion caused an increase in serum malonyldialdehyde (MDA) from baseline (100%) to 130.04?14.00% and 169.16?32.49% and ?-glucuronidase (?-g) activity to 191.05?86.71% and 242.54?49.09% at 3 and 6h, respectively. The bronchoalveolar lavage fluid (BALF) ?-g activity, lung MDA and extravascular lung water also significantly increased (P

Perception, affection and consciousness are basic psychological functions of human being. Affection is the subjective reflection of different kinds of objects. The foundation of human being's thinking is constituted by the three basic functions. Affective computing is an effective tool of revealing the affectiveness of human being in order to understand the world. Our research of affective computing focused on the relation, the generation and the influent factors among different affections. In this paper, the affective mechanism, the basic theory of affective computing, is studied, the method of acquiring and recognition of affective information is discussed, and the application of affective computing is summarized as well, in order to attract more researchers into this working area.
Affect ; Computing Methodologies ; Emotions ; Humans ; Mental Processes

Objective To study the relationship between soluble CD147 (sCD147) level in peripheral blood and serum lipid level and explore the effect of sCD147 on atherosclerosis in rheumatoid arthritis (RA). Methods The level of sCD147 in 36 patients with RA,36 patients with coronary artery disease (CAHD) and 30 healthy volunteers was detected by enzyme linked immunosorbent assay (ELISA) .The disease activity score (DAS28) in RA patients was evaluated and the correlation between sCD147 level and DAS28 score was analyzed.The serum lipid level of RA patients was detected by an automatic biochemical analyzer and the cor relation between sCD147 level and serum lipid level was analyzed.Results The level of sCD147 in serum of RA patients was significantly higher than that in patients with CAHD and healthy volunteers,sCDI47 level in the RA group with high DAS28 score was significantly higher than that with low or medium DAS28 score.In RA patients,elevated total cholesterol (TC) and triglyceride (TG) level was positively correlated with serum sCDI47 level (r=0.84,P＜0.05;r=0.87,P＜0.05;while slightly elevated,normal TC and normal TG had no correlation with serum sCDI47 level (r=0.41,P=0.21;r=0.14,P=0.57;r=0.49,P=0.87).Elevated or slight ly elevated LDL-C was positively correlated with serum sCD147 level (r=0.86,P＜0.05;r=0.81,P＜0.05), while no correlation could be found in the group with normal LDL-C level (r=0.78,P=0.22).The high density lipoprotein-cholesterol (HDL-C) level decrease in RA patients had no correlation with serum sCD147 level (r--0.04,P=0.96;r=0.13,P--0.87).Conclusion sCD147 may be involved in the pathogenesis of RA and associate with disease activity.Elevated sCD147 level may be associated with abnormal serum lipid in RA.

Objective:To evaluate the effects of several kinds of surface treatment methods on the bond strength of zirconia prosthesis,and to provide references for improving the bond strength of zirconia prosthesis.Methods:Sixty zirconia blocks(10 mm×10 mm×2 mm) were divided into 6 groups according to the surface treatment methods:sandblasting,silica coating,Z-PRIME Plus,sandblasting+Z-PRIME Plus,sandblasting+silica coating,and silica coating+Z-PRIME Plus groups.The zirconia-resin specimens were fabricated using ScotchbondTM Universal;shear bond test was performed to detect the shear bond strength after treated with water storage(37℃) for 24 h by universal mechanical testing mechine;the fracture types were observed by stereoscopic microscope.One fracture specimen was randomly chosen from each group,and the morphology of the specimen was examined under scanning electron microscope.Results:The shear bond strength of the specimen in Z-PRIME Plus group was higher than those in the other groups(P<0.05).The adhesive failure was predominantly observed in sandblasting,silica coating,sandblasting+silica coating and silica coating+Z-PRIME Plus groups.There were 4 cases of mixed failure in Z-PRIME Plus group and there were 8 cases of mixed failure in sandblasting+Z-PRIME group.No cohesive failure was observed in all the groups.The scanning electron microscope results showed that the fracture surface occurred on the resin-zirconia interface of the specimens in sandblasting group,Z-PRIME Plus group,and sandblasting+Z-PRIME Plus group.The silica-zirconia fracture of specimens occurred in silica coating group and silica coating+Z-PRIME Plus group.The silica-resin interface fracture of specimens partially occurred in sandblasting+silica coating group.Conclusion:The application of Z-PRIME Plus can significantly improve the bond strength between zirconia and resin.

Objective This study was carried out in order to analyze genetic diversity in plants of Dendrobium Sw. and compare the marker index (MI) between SRAP and RAPD markers. Methods Using 40 SRAP primer combinations and 36 RAPD primers,the genetic diversity of the materials in Dendrobium Sw. was amplified. Results A total of 1 977 loci were generated by 40 SRAP primer combinations,of which 90.2% was polymorphic and the genetic similarity (GS) ranged from 0.330 2 to 0.789 2. As for RAPD,281 bands were obtained,in which 87.1% was polymorphic,and the GS was from 0.494 2 to 0.773 1. There were some differences existing in the two cluster results revealed by SRAP and RAPD markers,they were as follows:compared to that of RAPD,the result of SRAP could more comfortably reflect the kinship and geographical origin,simultaneously conform to the traditional classification. For the experiment materials,SRAP (16.46) got an MI value 4.5 folds higher than that of RAPD (3.67),which meant SRAP markers were more effective in detecting genomic polymorphisms in the plants of Dendrobium Sw. Conclusion These results show that SRAP and RAPD could be applied to detecting genetic diversity analysis of plants in Dendrobium Sw. SRAP Also shows great superiority and advantage in this study.

Objectives To explore the effect of mibefradil, a kind of novel calcium channel antagonists, on proliferation of human pulmonary artery smooth muscle cells (HPASMCs) induced by platelet-derived growth factor (PDGF). MethodsHPASMCs were culturedin vitro, and randomly divided into control group, PDGF group, Mib group, and PDGF+Mib group. The PDGF group was stimulated by 25 ng/ml of PDGF. Mib group was intervented by 10 μmol/L of Mib. PDGF+Mib group was treated by PDGF and Mib. The reproduction rate in 48 hours and 72 hours were detected by MMT. Cell cycle was detected by lfow cytometry. The expression of proliferating cell nuclear antigen (PCNA) was observed by immunolfuorescence staining (IFS).ResultsThere were statistical differences among four groups in both 48 hours and 72 hours (P all??0.05). There were statistically differences of G0/G1 phase and S phase among four groups (P?