Objective To understand the clinical effect of conservative lapamscopic surgery with stripping corpus luteum for fallopian pregnancy to prevent persistent ectopic pregnancy.Methods From January 2005 to Augnst 2008.82 cases of fallopian pregnancy (6 cases for the second time) were performed conservative operatio through laparoscopic surgery with stripping corpus luteum.Seventy one cases underwent salpingostomy to remove the embryo, and other 11 cases were performed with extrusion of embryo sac at ampulla.The level of serum progesterone,β-HCG were measured before and after the therapy.All patients were followed up for one year.Results All patients'surgery had been successfully done through laparoscopie surgery.The duration of stripping corpus luteum was 5 to 10 minutes.The serum β-HCG(Mean±SD:(1213.51±118.84)U/L)and progesterone(Mean±SD:(3.25±2.44)nmol/L)arer operation were significantly lower than before the therapy(Mean±SD:(4267.86±983-56)U/L.(13.71±6.24)nmol/L,respectively)(P<0.01).The decrease of β-HCG value wag significantly correlated with the decreasing of progesterone(r=0.697,P<0.05).Only one patient had a persistent ectopic pregnancy.Fallopian examination through hysterosalpingography after 3 months was unobstructed in 71.1%(32/45 cases)and oh-strueted in 7 cases(15.6%).After one year ofthetherapytwo patients hadectopic pregnancy atthe same fallopian tube.Conclusions For females who have reproductive requirements with experience of fallopian pregnancy,conservative operation through laparoscopic surgery with stripping corpus luteum is safe and effective,and might have an important clinical effect to prevent postoperative persistent ectopic pregnancy.

0.05) . NNS could decrease blood viscosity and hematocrit, inhibit the aggregation of erythrocytes and platelet and increase the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH -Px). [Conclusion] NNS has an effect on AIS, which is similar to nimodipine. Its therapeutic mechanism may be related to the relief of cerebral ischemia by improving blood rheology and cerebral blood flow and protecting brain cells from injury by eliminating free radicals.

[Objective] To explore the protective mechanism of Naoxing Nasal Spray (NNS) on ischemic cerebral injury. [Methods] Seventy Wistar rats were randomized into 7 groups: high-dose NNS group (group A), moderate-dose NNS group (group B), low-dose NNS group (group C), nimodipine group (group D), model group (group E), solvent control group (group F) and pseudo-operation group (group G). Rat models of ischemia/reperfusion were established by blocking left middle cerebral artery. Plasma levels of tumor necrosis factor a (TNF-?) and interleukin 1? (IL-1?), malondialdehyde (MDA) content, activities of superoxide dismustase (SOD), glutathione peroxidase (GSH-Px) and nitric oxide synthase (NOS) in brain homogenate were detected. [Results] NNS decreased the contents of MDA, TNF-? and IL-1?, and increased the activities of SOD, GSH-Px and NOS. [Conclusion] NNS has protective effect on ischemic cerebral injury by promoting the clearance of free radicals, inhibiting the release of inflammatory mediator and increasing the synthesis of NO and thus to reduce injury of free radicals, inhibit the local inflammatory reaction and improve cerebral blood perfusion.

Objective·To evaluate the clinical value of two-dimensional ultrasound combined with four-dimensional color Doppler ultrasound in the diagnosis of uterine space-occupying lesion.Methods·A total of 75 patients with uterine space-occupying lesion were enrolled in this study from March 2015 to February 2016 at Hospital of Southwest Medical University.Patients received both two-dimensional ultrasound and four-dimensional color Doppler ultrasound to determine the location,number and nature of the lesions,as well as the clinical staging of malignant lesions,then the diagnostic accuracy was evaluated.Results·Among 75 patients,there were 26 cases with endometrial carcinoma,21 cases with hysteromyoma,16 cases with metropolypus,and 12 cases with uterine adenomyosis.The diagnostic accuracy of two-dimensional ultrasound combined with four-dimensional color Doppler ultrasound for the uterine space-occupying lesion was 88.0％,which was significantly higher than that of two-dimensional ultrasound (64.0％) (P＜0.05).What's more,the diagnostic accuracy of two-dimensional ultrasound combined with four-dimensional color Doppler ultrasound for the clinical staging of endometrial carcinoma was 88.5％,which was significantly higher than that of two-dimensional ultrasound (61.5％) (P＜0.05).Conclusion·Two-dimensional ultrasound combined with four-dimensional color Doppler ultrasound is more effective to improve the diagnostic accuracy of uterine space occupying lesion and clinical staging of endometrial carcinoma in comparison with two-dimensional ultrasound,which will favor the clinical strategy for the treatment of uterine space-occupying lesion.

Objective:Adopting RP-HPLC method,to study and establish the fingerprint of smilacis rhizome.Methods: YMC-Pack ODS-A(5 ?m,4.6 mm?250 mm) chromatographic column,acetonitrile-0.05% phosphoric acid solution gradient elution were applied;with flow rate of 0.8 ml/min and UV detector at 290 nm.Results: Technology investigation indicated that the analytical method this study established has a better reproducibility.The similarity of smilacis rhizoma fingerprints from different sources is better;smilacis rhizome and its three confusions fingerprints are apparently different.Conclusion: HPLC fingerprint analysis can be a method for quality control of medical material of smilacis rhizoma.

BACKGROUND: Hemorrhagic cystitis (HC) is one of common complications in patients undergoing hematopoietic stem cell transplantation (HSCT). It is of great value for improvement in the HSCT outcome to describe the clinical characteristics of HC and risk factors. OBJECTIVE: To investigate the incidence of HC in children after HSCT, and to analyze its clinical characteristics and risk factors.DESIGN: Case analysis SETTING: Center of Hematopoietic Stem Cell Transplantation, Department of Pediatrics, Second Affiliated Hospital of Sun Yat-sen University.PARTICIPANTS: Experiments were performed at the Center of Hematopoietic Stem Cell Transplantation, Department of Pediatrics of Second Affiliated Hospital of Sun Yat-sen University from October 1998 to June 2004. Eighty-eight patients receiving umbilical cord blood transplantation (UCBT) and peripheral blood stem cell transplantation (PBSCT) were enrolled; 49 were males and 39 were females. The age ranged from 2 to 18 years with an average of 8.0 years. Guardians of child patients signed informed consents. The experimental procedures were approved by Medical Ethics Committee.METHODS: ①Conditioning regimens included combination of cyclophosphamide (CY, 120-200 mg/kg) with busulphan (BU, 14-20 mg/kg)-based chemotherapy and combination of CY with total body irradiation (TBI, 2-8 Gy) or total lymphoid irradiation (TLI, 2-8 Gy)-based radiotherapy. ②HC was defined according to the criteria proposed by references 7 and 8. The incidence, clinical characteristics, laboratory examination, treatment and outcome for HC were described. The association of various clinical factors including age, gender, human leucocyte antigen (HLA) typing, diseases for transplant, the type of stem cell, the type of transplantation, the occurrence of acute graft-versus-host disease (aGVHD) and cytomegalovirus (CMV) infection with the development of HC were examined.MAIN OUTCOME MEASURES: ①Incidence of HC, ②HC patient characteristics and laboratory examination, ③HC treatment and outcome, and ④risk factors analysis. RESULTS: All 88 patients were included in the final analysis. ①The incidence of HC: 16 patients (18.2%, 16/88) developed HC post-transplant with the severity graded as mild in 11 cases (68.7%) and severe in 5 cases (31.3%). ②HC patient characteristics and laboratory examination: All had hematuria and 8 cases (50.0%) had typical pollakisuria, urinary urgency, odynuria and gross hematuria; 10 cases (62.5%) had gross hematuria and 11 had proteinuria (+ to +++); Leucocytes were detected in 7 cases. ③Treatment and outcome: All patients recovered at a median of 13.5 days (range 2-53 days). ④Risk factors analysis: The incidence of HC was significantly higher in the group of ≥ 6 years old, presence of aGVHD and development of cytomegalo-virus (CMV) infection (P < 0.05-0.01). CONCLUSION: ①HC has its own clinical characteristics following HSCT in children but with good prognosis. ②The risk factors for HC are ≥ 6 years old, presence of aGVHD and CMV infection.

BACKGROUND: Brain-awakening nasal sprayer is composed of many herbs,such as Chuanxiong and Shichangpu, which were regarded by "Shennong Bencaojing" as having the function of "preventing stroke in the brain".OBJECTIVE: To observe the changes of free radicals and nitric oxide synthase in rat brain following focal ischemic-reperfusional injury due to brain-awakening nasal sprayer intervention and compare with that due to classical nimodipine.DESIGN: A randomized controlled study.SETTING: Department of internal medicine of a hospital affiliated to a traditional Chinese medical university.MATERIALS: Seventy adult male Wistar rats of clean grade, were randomly divided into seven groups: brain-awakening nasal sprayer of higher dosage group, moderate dosage group, lower dosage group, nimodipine intraperitoneal injection group, physical saline nasal sprayer group, menstruum nasal sprayer group, and sham operation group with 10 rats in each.METHODS: Focal brain ischemia-reperfusion model was established by blocking the left cerebral middle artery in rats of all the groups except sham operation group. Three days before model establishment and during reperfusion, rats were given different dosages of brain-awakening nasal sprayer composed of Chuanxiongqin and Shichangpu of 5.4, 2.7, 1.08 mg/(kg · d) and 1.35, 0. 54, 0.27 g/(kg· d), respectively, three times a day; which was replaced by physical saline and menstruum nasal sprayer of 0. 18 mL/ (kg · d),three times a day in physical saline nasal sprayer group and menstruum nasal sprayer group; rats in nimodipine intraperitoneal injection group received intraperitoneal injection of nimodipine by 0. 8 mg/(kg · d) twice a day. Rats in sham operation group were routinely raised. The content of prodialdehyde, superoxide dismutase and nitric oxide synthase were measured with colorimetric method.MAIN OUTCOME MEASURES: ① The changes of prodialdehyde content, superoxide dismutase and nitric oxide synthase activity in rat brain following focal ischemic-reperfusional injury in groups of different dosage of brain-awakening nasal sprayer and other groups. ② Comparison between different dosage brain-awakening nasal sprayer intervention groups and nimodipine intraperitoneal injection group.RESULTS: Eight rats died during model establishment and the other 62 rats entered the results analysis. ① Content of prodialdehyde: It was significantly lower in higher dosage group and nimodipine intraperitoneal injection group than in physical saline nasal sprayer group [ (0.92 ± 0. 32), (0. 87 ± 0. 39)vs(1.35 ±0. 34) μmol/g, P ＜ 0.05], but there was no difference between higher dosage group and nimodipine intraperitoneal injection group. ② Activity of superoxide dismutase: It was obviously higher in higher dosage group and nimodipine intraperitoneal injection group than in physical saline nasal sprayer group[ (35.64 ± 11.67), (33.88 ± 7. 15) vs(20. 70 ± 3.88) NU/mg,P ＜ 0. 05 ], but no difference could be observed between higher dosage group and nimodipine intraperitoneal injection group. ③ Activity of nitric oxide synthase and superoxide dismutase: It was found obviously higher in higher dosage group and nimodipine intraperitoneal injection group than in physical saline nasal sprayer group[ (4.64 ± 1.22), (5.00 ± 1.10) vs (3.08 ± 1.12) mkat/g, P ＜ 0.05], but no difference could be observed between higher dosage group and nimodipine intraperitoneal injection group.④ The activity of nitric oxide synthase and superoxide dismutase slightly increased while prodialdehyde slightly decreased in moderate dosage group,lower dosage group and menstruum nasal sprayer group, which did not differ significantly from physical saline nasal sprayer group.CONCLUSION: Brain-awakening nasal sprayer intervention exerts multiple effects such as preventing lipo-peroxidation following brain ischemic- reperfusional injury, in addition to suppressing prodialdehyde production, attenuating injury induced by free radicals and increasing nitric oxide synthase activity, thereby playing a similar role to nimodipine in protecting against brain ischemic-reperfusionaldamage

Objective To investigate the clinical efficacy of TCM external treatment combined with Dasangju Mixture in treating mycoplasma pneumoniae pneumonia (MPP) in children. Methods One hundred patients with MPP were randomly divided into control group and treatment group by parallel design, with 50 patients in each group. Patients in control group orally took azithromycin combined with other symptomatic treatment when necessary, 7 d for a course and continuously for 3 courses. Based on the treatment for control group, patients in treatment group were added with TCM external treatment (BL13 and RN17) combined with Dasangju Mixture, while azithromycin was sequentially used just for 2 courses. Other types of treatment lasted for 3 courses. The improvement time of symptoms and signs of the patients were recorded and the therapeutic effects of the two groups were evaluated. Moreover, the serum concentrations of mycoplasma pneumoniae-immune gloulin M (MP-IgM) were determined before and after treatment on the 1st and 21st days. Results The improvent time of fever, and cough and lung sign of the treatment group were more obviously shortened than the control group (P<0.05). The total effective rate was 96.0% (48/50) in observation group and 74.0% (37/50) in control group, with statistical significant difference between the two groups (P<0.05). After treatment, the negative rate of the serum concentration of MP-IgM in the treatment group was 82%, and that of the control group was 58%, with statistical significance between the two groups (P<0.05).Conclusion TCM external treatment combined with Dasangju Mixture can not only significantly improve the clinical symptoms and signs of MPP children patients, but also shorten the disease course, and the efficacy was significant. Meanwhile it can reduce the course and side effects of azithromycin, which provides reference to the administration of antibiotics in children.

Objective To study the preparation method and analytical technique of hyperoside from Flos Abelmoschus manihot.Methods Hyperoside was isolated and purified by solvent extract and chromatography, whose structure was determined by 1H-NMR and 13C-NMR. The purity was analyzed by TLC and HPLC.Results The TLC showed that the hyperoside had no impurity spot. The HPLC indicated that the purity reached more than 98.5%.Conclusions The mothod of isolation and purification for hyperoside reported in this paper was simple and economical.

Objective To study the effects of Naoxing Nasal Spray(NNS)on ultrastructure of brain tissues i n rats with focal cerebral ischemia -reperfusion inj ury(CIRI ).Methods Thirty Wistar rats were randomly all ocated to 6groups:Group A(treated with high dose of NNS ),Group B(treated with moderate dose of NNS),Group C(treated with low dose of NNS ),Group D(positive control group),Group E (treated with normal saline)and Group F (normal group).After medication for 3days,rat models of CIRI were established by the occlusion of left mid dle cerebral artery.Ultrastructur e of left fore-head cortical tissue was observed un der electron microscope after one -h our ischemia and 24-hour reperfusio n.Results The injury of neurons,gliocytes and capillaries in Group A and Group B was obviously milder than that in Group E and that in Group A was the mildest,the differences being not significant as compared with that in Group D.Conclusion NNS can reduce the ischemic damage of cerebr al cortex and obviously protect cere bral tissues in rats with CIRI.