OBJECTIVE:To study the protective effects of Shenqi fuzheng injection on ischemia/reperfusion injury of the lungs of dogs following cardiopulmonary bypass(CPB).METHODS:The CPB dog model was induced,then the model dogs we-re randomly divided into three groups:control group,Shenqi fuzheng injection high dosage group,and Shenqi fuzheng injection low dosage group. The lung tissue samples of 1.0 cm3 from the same lobes of lung were obtained at 10 min before CPB and 60 min and 120 min after reperfusion,respectively for determination of the contents of superoxide dismutase(SOD),malondialdehyde (MDA),wet / dry ratio of lung (W/D) etc. The femoral arterial blood gas analysis was performed in two groups at 30 min and 120 min after reperfusion for calculation of oxygenation index(OI) and respiratory index(RI).The lung tissue samples(1.0 cm3) from the same lobes of lung were obtained at 120 min after reperfusion for HE staining and observation of pathological changes under light microscope. RESULTS:In the control group,OI were significantly decreased but RI were sig-nificantly increased at 120 min after reperfusion as compared with at 30 min after reperfusion; in Shenqi fuzheng injection high dosage group at 30 min and 120 min,OI was significantly higher but RI was significantly lower than in control group. In the control group at 60,120 min after reperfusion,the SOD content were decreased significantly,MDA content and W/D ratio in lung tissues were increased significantly compared with at 10 min before CPB; in Shenqi fuzheng injection high dosage group at 60 min and 120 min,MDA content and W/D ratio were significantly lower but SOD content was significantly higher than in the control group.The pathological changes at 120 min after reperfusion showed that there were severe leukocyte infiltration and pulmonary edema in the control group,but the pathological changes in the Shenqi fuzheng injection high dosage group were milder than in the control group. CONCLUSION:Administration of Shenqi fuzheng injection in dog during CPB has beneficial protective effects against I/R lung injury,and the effect may be associated to its function in alleviating the damage induced by oxygen free radicals in lung tissues.

Objective To study the differences between the mandibular movement in empty opening and protrusion trace of the skeletal anterior reverse malocclusion before and after the early protraction treatment in early stage of mixed dentition. Methods ACRUSdigma system, a kind of ultrasonic mandibular movement record system, was used in the study. The kinematic center which can represent the translation and rotation of the condylar and the incisal points were used as reference point to compare the difference of the empty opening and protrusion mandibular movement trajectory of 30 individuals with skeletal anterior reverse bite malocclusions before and after the early protraction facemask treatment. Results For the kinematic center, the typical trajectory was changed, the upper movement disappeared and the distances between vertical and horizontal movements were increased at the end of the treatment in the empty opening movement on the sagittal plane. In the empty protrusion movement, the interplanar distance of the kinematic center was increased significantly on the sagittal plane. For the incisial point, it moved ahead at first and then downward vertically in the empty protrusion movement, and the interplanar distance was increased significantly on the sagittal plane in the empty opening movement. Conclusions By giving the protraction treatment, the relation of upper mandible and submaxilla of the skeletal anterior reverse bite malocclusions is changed. During the later stage of the treatment, for the occlusal splint was ground off, the normal occlusial inducement is built, which affords the rebuilding of temporomandibular joint (TMJ), ligaments and muscles. The trajectory also becomes normal. The normal trajectory may supply a better environment for the development and rebuilding of jaw, TMJ, ligaments and muscles.

Objective:To evaluate the recovery effect of early enteral nutrition (EEN) in elderly patients with severe chest trauma.Methods:83 elderly patients with severe chest trauma were divided randomly into two groups,research group(46 patients) and control group(37 patients).In both groups the nutrition indicator,the complication and the recovery time were detected on admission (T1),the 1st week (T2) and 2ed week (T3) after admission.Results:After 2 weeks,the level of nutrition indicator in research group was significantly higher than in control group,and the incidence of complication was significantly lower than in control group.Conclusion:Early enteral nutritional support is helpful for the nutritional support and reduce the support of complication in elderly patients with severe chest trauma.

Objective:To study TGF? and TGF?3 polymorphisms in patients with nonsyndromic cleft lip with or without cleft palate(NSCLP,CLP or CL)and cleft palate only(CPO).Methods:TGF? and TGF?3 DNA was extracted and amplified from peripheral leukocytes of 56 cases of NSCLP(40 of CLP and 16 of CL),26 of CPO and 28 of unrelated controls.The primers were designed according to the 3'untranslated region of TGF? and 5th exon of TGF?3 published in the Internet.The PCR products were analyzed by single-stranded conformation polymorphism(SSCP).The aim fragments were further conformed by DNA sequencing after being cloned into pGEM-T vector.Results:The 345 bp fragment of TGF? and 193 bp of TGF?3 were amplified from NSCLP,CPO and control samples.In SSCP analysis,three alleles of TGF? and two of TGF?3 were found.Sequencing results showed three DNA polymorphic sites in TGF? and one in TGF?3 which were all base shifts.There was no difference of each genome between patients and controls.Conclusion:There are no direct association between 3'UTR of TGF? or 5th exon of TGF?3 and NSCLP or CPO in Chinese.

Objective: To evaluate the influence of fusing conditions on the surface structure and morphology of the SP1 SiO2. Methods:The SP1 SiO2 samples were sintered under 500, 650, 800, 950 and 1 100 ℃ at a rate of 250 ℃/h and maintained for 10, 30 min, 3 h respectively. The surface structure was characterized by FT-IR. Results:The characteristic peak of Si-OH bond at 958 cm-1 took off gradually with the rising temperature and prolonged time. Under the conditions of 950 ℃ maintained for 10 min and 30 min, and 650 ℃ maintained for 3 h, it disappeared completely. The particles of the SP1 SiO2 became larger while the size distribution became narrow. Conclusion: Fusing conditions can affect the Si-OH content and the size of the SP1 SiO2, as well as the size of distribution.[

Objective To explore the efficacy and safety of Linagliptin in combination with Metformin in newly diagnosed T2DM patients. Methods A total of 140 newly diagnosed T2DM patients were enrolled in this study. All the patients were admitted in the Endocrinology and Metabolism department of our hospital from January 2013 to October 2013. Random number table method was used in patients’ selection. All the patients were divided into two groups :Linagliptin plus Metformin group (Linagliptin group ,n=70) and Glipizide plus Metformin group (Glipizide group ,n=70) ,and followed up for 12 weeks. Efficacy and safety of treatment were compared between the two groups. Results The difference of FPG ,2 hPG and HbA1 c did not reach the statistical significance between the two groups at baseline treatment (P>0.05). FPG ,2 hPG and HbA1 c were significantly lower after treatment compared with baseline in both groups (P<0.05). FC-P ,2 hC-P ,HOMA-β,HOMA-IR ,IAI and adiponectin level at baseline were similar between the two groups ( P> 0.05 ). All these indicators were improved after treatment in both groups (P< 0.05) ,and were significant better in Linagliptin group than in Glipizide group. Adverse events rate were significantly higher in Glipizide group than in Linagliptin group (18.57%vs 5.71% ,χ2 = 5.423 ,P= 0.020 ). Conclusion The efficacy of Linagliptin in combination with Metformin in newly diagnosed T2DM patients was similar with Glipizide plus Metformin. The β cell function improvement was better and APN level was higher after Linagliptin treatment.

Objective To observe the effects of Zhennao-Ning capsule on cerebral microcirculation.Methods Cerebral hypoperfusion model of mices was induced by right common carotid arteries ligation method,The cerebral blood flow for 5min was recorded under normal circumstances comand for 30min when modeled,and then calculate the rate of blood flow changes.Microcirculatory impairment model of rats was induced by intravenous injection of high molecular dextran(HMD).The time of the emergence of microcirculatory impairment was recorded.Vascular flow,blood flow pattern and the number of outlets in the same range were recorded.Results The blood flow was significant decrease in the cerebral hypoperfusion model mice,when compared with the control mice.Zhennao-Ning capsules at the dosage of 1500 mg/kg、750 mg/kg treatment could improve the cerebral blood flow of rats at 10,20,and 30 min after modeled (56％,51％,50％ and 42％,49％,51％,respectively,P＜0.01).Zhennao-Ning capsules at the dosage of 600 mg/kg,300 mg/kg could significantly reduce the change of blood flow pattern(P＜0.01),improve vascular flow rate (26％,36％,P＜0.05 or P＜0.01),extend the time of the emergence in the microcirculatory impairment model rat (53％,34％,P＜0.01).Conclusion Zhcnnao-Ning capsules could improve microcirculatory impairment and partly cure the clinical migraine disease.

OBJECTIVEWe used functional magnetic resonance imaging (fMRI) to explore the effects of noxious coldness and non-noxious warmth on the magnitude of cerebral cortex activation during intraoral stimulation with water.

METHODSSix male and female subjects were subjected to whole-brain fMRI during the phasic delivery of non-noxious hot (23 °C) and no- xious cold (4 °C) water intraoral stimulation. A block-design blood oxygenation level-dependent fMRI scan covering the entire brain was also carried out.

RESULTSThe activated cortical areas were as follows: left pre-/post-central gyrus, insula/operculum, anterior cingulate cortex (ACC), orbital frontal cortex (OFC), midbrain red nucleus, and thalamus. The activated cortical areas under cold condition were as follows: left occipital lobe, premotor cortex/Brodmann area (BA) 6, right motor language area BA44, lingual gyrus, parietal lobule (BA7, 40), and primary somatosensory cortex S I. Comparisons of the regional cerebral blood flow response magnitude were made among stereotactically concordant brain regions that showed significant responses under the two conditions of this study. Compared with non-noxious warmth, more regions were activated in noxious coldness, and the magnitude of activation in areas produced after non-noxious warm stimulation significantly increased. However, ACC only significantly increased the magnitude of activation under noxious coldness stimulation.

CONCLUSIONResults suggested that a similar network of regions was activated common to the perception of pain and no-pain produced by either non-noxious warmth or noxious coldness stimulation. Non-noxious warmth also activated more brain regions and significantly increased the response magnitude of cerebral-cortex activation compared with noxious coldness. Noxious coldness stimulation further significantly increased the magnitude of activation in ACC areas compared with noxious warmth.

Objective To determine the expression and clinical value of neuropilin-1 (NRP-1) in hepatocellular carcinoma (HCC).Methods One hundred and fifty-one cases of HCC tissues and 89 cases of healthy liver tissues were chosen to compare the expression of NRP-1 by immunohistochemistry.Then the relationships between different clinical factors and the expression of NRP-1 were analyzed by univariate and multivariate statistical analysis.Moreover,the survival rates were compared by survival analysis between different expressions of NRP-1 in HCC patients.Results Eleven cases were lost to follow-up or died for non HCC disease,and the effective cases in the final study were 140 cases.The positive expression rates of NRP-1 in HCC and normal liver tissues were 65.00％ and 35.96％ respectively,and the difference was statistically significant (x2 =18.843,P ＜0.001).According to the expression level of NRP-1,140 patients with HCC were divided into negative expression group (n =49) and positive expression group (n =91).Univariate analysis showed that the expression of NRP-1 in HCC was correlated with tumor number (x2 =8.025,P =0.005),TNM stage (x2 =26.467,P ＜ 0.001),differentiation degree (x2 =15.296,P ＜ 0.001),portal vein invasion (x2 =9.054,P =0.003) and hepatic vein invasion (x2 =5.928,P =0.015).Multivariate statistical analysis showed that TNM stage (OR =1.392,95％ CI:1.121-1.730,x2 =8.950,P =0.003),differentiation degree (OR =1.469,95％ CI:1.102-1.958,x2 =6.862,P =0.009),portal vein invasion (OR =1.829,95％ CI:1.157-2.893,x2 =6.665,P =0.010) and hepatic vein invasion (OR =2.161,95％ CI:1.172-3.987,x2 =6.084,P =0.014) were important factors for NRP-1 expression.The median survival time of NRP-1 negative HCC patients was significantly longer than that of positive group (44 months vs.23 months),and the difference was statistically significant (x2 =21.922,P ＜0.001).Conclusion NRP-1 is over-expression in HCC tissue and related to the malignant progress of HCC,and this suggests poor prognosis in patients with HCC.

The aim of the study was to observe osseointegration of dental implants in osteoporotic rats. Rats were randomly divided into groups A(SHAM) and B(OVX), and pure titanium screws were implanted in the distal half of the right femurs. 1 5 and 3 months after implantation, half of the rats in each group were sacrificed and right femurs were observed by X ray photography, scan electron microscopy as well as light microscopy. The results showed that 1.5 and 3 months after implantation, the osseointegration index (OI) of group A was higher than that of group B. But the OI of each group was almost the same at different intervals. 1 5 months after implantation, the amount of new bone formation in group A was more abundant than that in group B, and it increased in group A but decreased in group B 3 months after implantation.The results confirmed that with higher BMD osseointegration was better. Osteoporotic changes could decrease OI and the amount of new bone formation at implant bone interface in rats. Osseointegration would not improve without outside factors.