1.THE PREVENTIVE EFFECTS OF VITAMIN E ON DECOMPRESSION SICKNESS IN MICE
Academic Journal of Second Military Medical University 1982;0(02):-
Ninety two male mouse (weight 22?3g) were randomly divided into test and control groups Vitamin E and rqual volume excipient were given i. m. respectively. The concentration of vitamin E in serum of test group was twice as thatof control group. These two groups were compressed simultaneously to 14kgf/cm2 with compressed air at the rate of 2kgf/cm2 per minute for 50 minutes, anddccompressed to surface in 7 minutes. The results showed : the morbidity of serious ease and the mortality in test group were lower than that in the control group, and the reduction of circulating platelct number is less than that in control group. These results suggested that vitamin E has protective effects on mice decompression sickness.
2.STUDY ON THE FACTORS AFFECTING THE PREVALENCE AND EPIDEMIOLOGIEAL PTTERN CF SCHISTOSOMIASIS JAPONICA IN THE MARSHLAND AND LAKE REGIONS
Chinese Journal of Schistosomiasis Control 1989;0(02):-
A 4-year study on the factors affecting the prevalenee and epidemiological pattern of schistosomiasis japonica around Dongtin and Boyang lakes was reported. A proposal was made on the division of the endemic areas of the lake and marshland regions into different types,a definition about the high-risk places and the principles of their classification.This study also elucidated the main reservoirs of different types of endemic areas and the relationship between human exposure and infection. On the basis of the effects of prevention and treatment and cost-effect analysis in the 8 field study units, the goal for the control of schistosomiasis in the lake region for the next 3~5 years has been devised.A preliminary mathematical model of human and domestic animals as the main reservoirs of infection has been set up.
3.STUDY ON THE HUMAN WATER CONTACT AND SOCIOECONOMIC FACTORS IN ENDEMIC AREA OF SCHISTOSOMIASIS JAPONICA
Ming CHEN ; Hongchang YUAN ; Qingwu JIANG ;
Chinese Journal of Schistosomiasis Control 1989;0(01):-
A quantitative study on human water contact was carried out in an endemic area of schistosomiasis japonica. The information relating to frequency, duration and intensity of each activities was obtained from 390 persons (93% of all) aged 5-59 and 10 days of each season in 1987 was surveyed by using systematic sampling. Socioeconomic investigation and stool examination were also performed on the same population. It is found that cutting grass and fishing are the most important activities, as well as swimming and bathing. The accumulated index ofexposure is high in spring and summer, low in autumn and rare in winter. The peak contact is 8-12 o'clock in the morning. The reasons of contact are different between males and females. And the peak contact is at teen aged youth. Studies on water contact and socioeconomic factors show that there are more contacts in peasants than in those with other occupations. Rich farmers who has more savings contact less. The contacts seem more frequent in those whose family water supply is from infected water. The stepwise regression analysis shows that the most important factor relating to the infection of schistosomiasis japonica are index B of exposure (accumulation of duration ? intensity). The infected water supply of family and education were two other factors relating to the infection.
4.Effect of Chinese and western medicine integration on spinning behavior of rats with Parkinson disease
Jiancheng HE ; Canxing YUAN ; Hongchang WEI ; Ruxing CHEN
Journal of Integrative Medicine 2003;1(4):293-5
OBJECTIVE: To observe the effect of Chinese and western medicine integration on the spinning behavior of rats with Parkinson disease. METHODS: Model of the lateral Parkinson disease was made with injection of 6-hydroxydopamine (6-OHDA) into the black substance of the right side of the brain, and the model rats were treated with madopar and Chinese herbs for nourishing the liver and kidney, clearing collaterals and detoxification. The rat's spinning behavior was observed, and was compared with the normal control group, madopar group and sham-operation group at the same time. RESULTS: Chinese and western medicine integration could obviously reduce the spinning circles of the rats. CONCLUSION: Chinese and western medicine integration can significantly improve the spinning behavior of the model rats.
5.LONGITUDINAL EVALUATION OF EFFICACY ON CONTROL OF SCHISTOSOMIASIS JAPONICA BY ENVIRONMENTAL MODIFICATION IN JIJIABA VILLAGE, ANHUI PROVINCE
Qingwu JIANG ; Hongchang YUAN ; Dumin ZHONG ; Min CHEN
Chinese Journal of Schistosomiasis Control 1989;0(02):-
A preliminary survey was done in 1980 in Jijiaba Village before the dike was built . The dike of 2. 5 km in length and 4m high was built along Baiyang River by the end of 1980 . The molluscicide NaPCP was used for snail control from 1981 to 1984 . After the entire control measures were completed in 1984, a follow-up survey was conducted as to measure the impact of control measures on prevalence of schistosomiasis and population of snail from 1985 to 1989 . All of the results suggested that these control measures were very successful. The transmission of schistosomiasis in this area has been prevented since 1984 .
6.Effect of madopar on nerve cell apoptosis of rats with Parkinson's disease
Jiancheng HE ; Canxing YUAN ; Hongchang WEI ; Ruxing CHEN
Chinese Journal of Geriatrics 2003;0(07):-
Objective To illustrate the effect of Madopar on nerve cell apoptosis of rats with Parkinson’s disease (PD). Methods Hemi-lateral PD model was made by injecting 6-OHDA into the black substance of the right side of the brain and treated with Madopar. At the same time,the normal group and sham-operation group were set up,and TUNEL staining and transmission electro-speculum were employed to observe the apoptosis of nerve cells. Results The TUNEL positive cells(23.50?3.85) were significantly less than the model group(37.83?6.46) and the comparison of apoptosis cells counting showed marked difference( P
7.The model of training the trainers in community general practice
Qian CHEN ; Tianhao WANG ; Jie GU ; Yuan ZHANG ; Shanzhu ZHU ; Hongchang WANG ; Liping XU
Chinese Journal of General Practitioners 2013;(7):525-528
Objective To evaluate a normative and systematic training mode for teachers of community general practice.Methods A total of 16 general practice teachers,who came from Fenglin community health service centre in Shanghai,participated three-stage training mode during March 2011 to February 2012.The clinical competence evaluation and questionnaire survey were conducted before and after training.Fifty trainees trained by the teachers also received clinical competence evaluation.Results After the three-stage training,the test score in medical knowledge,physical examination and clinical skills of the teachers did not change significantly (P =0.794,0.674 and 0.326).The self assessment questionnaire survey of general practice teachers indicated a significant increase,especially in practice capability (t =-2.840,P =0.015) and overall quality (t =-3.017,P =0.011).After training by the teachers,the medical knowledge (t =-9.200,P =0.000),physical examination (t =-9.947,P =0.000) and clinical skills (t =-14.828,P =0.000) of 50 trainees increased markedly.Conclusions Differed from conventional training courses,the three-stage training enhances teaching ability and overall quality of community general practice teachers,and provides a effective training model.
8.Research progress of sarcolipin-a new regulatory protein of sarcoplasmic reticulum Ca2+ ATPase.
Baoping YUAN ; Rong LU ; Yanpin GU ; Yueling LIAO ; Hongchang WEI
Journal of Central South University(Medical Sciences) 2012;37(3):316-319
Sarcolipin (SLN) is a 3 kD membrane protein found in sarcoplasmic reticulum (SR). It has 31 amino acid residues; SLN and phopholamban (PLB) are belong to the same protein family, so they have similar physiological functions. SLN inhibits sarcoplasmic reticulum Ca(2+) ATPase (SERCA) activity and reduces its affinity of Ca(2+), resulting in dysfunction of myocardial contraction and heart failure. However, much remains to be elucidated. SLN independently or in conjunction with PLB affects SERCA activity, imbalancing intracellular calcium homeostasis, and reducing myocardial contractivity; these effects promote the development of heart failure.
Animals
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Calcium-Binding Proteins
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physiology
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Heart Failure
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physiopathology
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Humans
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Muscle Proteins
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metabolism
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physiology
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Myocardial Contraction
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physiology
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Proteolipids
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metabolism
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physiology
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Sarcoplasmic Reticulum
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metabolism
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Sarcoplasmic Reticulum Calcium-Transporting ATPases
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antagonists & inhibitors
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metabolism
9.Ultra-high dose rate irradiation induced DNA strand break in plasmid DNA
Hui LUO ; Qigang YUAN ; Phyllis ZHANG ; Leijie MA ; Ronghu MAO ; Hongchang LEI ; Yanan SUN ; Shuai SONG ; Xiaohui WANG ; Hong GE
Chinese Journal of Radiological Medicine and Protection 2023;43(3):161-167
Objective:To compare the effects on DNA strand break induced by ultra-high dose rate (FLASH) electron beam and conventional irradiation, and investigate whether FLASH effect was correlated with a reduction of radiation response.Methods:Aqueous pBR322 plasmid was treated with FLASH (125 Gy/s) and conventional irradiation (0.05 Gy/s) under physioxia (4% O 2) and normoxia (21% O 2). Open circle DNA and linear DNA were detected by agarose gel electrophoresis, and the plasmid DNA damage was quantified with an established mathematical model to calculate the relative biological effect (RBE) of DNA damage. In some experiments, Samwirin A (SW) was applied to scavenge free radicals generated by ionizing radiation. Results:Under physioxia, the yields of DNA strand breakage induced by both FLASH and conventional irradiation had a dose-dependent manner. FLASH irradiation could significantly decrease radiation-induced linear DNA compared with conventional irradiation ( t=5.28, 5.79, 7.01, 7.66, P<0.05). However, when the aqueous plasmid was pretreated with SW, there was no difference of DNA strand breakage between FLASH and conventional irradiation ( P>0.05). Both of the yields of open circle DNA and linear DNA had no difference caused by FLASH and conventional radiotherapy at normoxia, but were significantly higher than those under physioxia. In addition, the yields of linear DNA and open circle DNA induced by FLASH irradiation per Gy were (2.78±0.03) and (1.85±0.17) times higher than those of conventional irradiation, respectively. Conclusions:FLASH irradiation attenuated radiation-induced DNA damage since a low production yield of free radical in comparison with conventional irradiation, and hence the FLASH effect was correlated with oxygen content.
10.Phantom study based on MRI cine sequences: analysis of the accuracy of tumor motion range accuracy
Bing LI ; Yuan WANG ; Ronghu MAO ; Dong LIU ; Wenzheng SUN ; Xiangyue LIU ; Nan MENG ; Wei GUO ; Shuangliang CAO ; Xipan LI ; Chen CHENG ; Hui WU ; Hongyan TAO ; Dingjie LI ; Zhaoyang LOU ; Hongchang LEI ; Lingguang MENG ; Hong GE
Chinese Journal of Radiation Oncology 2024;33(12):1144-1151
Objective:To investigate the accuracy of magnetic resonance imaging (MRI) cine sequences in determining the range of tumor motion in radiotherapy, providing a basis for the precise delineation of the target volume in motion for radiation therapy.Methods:A modified chest motion phantom was placed in a MRI scanner, and a water-filled sphere was used to simulate a tumor. True fast imaging with steady precession (TrueFISP) MRI cine sequences from Siemens were used to capture the two-dimensional motion images of the simulated tumor. The phantom experiments were divided into three modes: head-foot motion mode, rotation motion mode, and actual respiratory waveform mode. In the head-foot motion mode, respiratory motion period (3, 4, 5, 6, 7 and 8 s), amplitude (5, 10 and 15 mm), and respiratory waveform of the simulated tumor (sin and cos4) were set, resulting in a total of 36 motion combinations. In the rotation motion mode, a cos4 waveform was used for respiration, with respiratory periods of 3, 4, 5, 6, 7 and 8 s, head-foot motion set amplitudes of 5, 10 and 15 mm, and anterior-posterior (AP) and left-right (LR) motion set amplitudes in three combinations ([2.5, 2.5] mm, [2.5, 5.0] mm, [5.0, 5.0] mm), resulting in a total of 54 motion combinations. In the actual respiratory waveform mode, respiratory waveforms of 5 randomly selected patients from Affiliated Cancer Hospital of Zhengzhou University were obtained. Under each motion combination, TrueFISP cine images (30 frames, with an acquisition time of 11 s per frame) were obtained. The code was used to automatically identify the two-dimensional coordinates of the center of the simulated tumor in each image, and sin and cos4 functions were separately employed to fit the tumor position in the motion direction, thereby obtaining the fitted motion period and amplitude. The difference between the maximum and minimum values of the tumor's center coordinates in the head-to-foot direction is taken as the range of movement, referred to as the calculated amplitude. For the actual respiratory waveform, the distance between the measured maximum and minimum positions is used to calculate the amplitude.Results:In the head-foot motion mode, the fitted amplitudes of both sin and cos4 waveforms deviated from the set amplitudes by 0-0.51 mm, with relative deviations of 0%-4.2%. The deviation range between the calculated amplitudes and the set amplitudes of the two waveforms were 0.08-0.94 mm, with relative deviations of 1.1%-6.3%. In the rotation motion mode, the fitted amplitudes deviated from the set amplitudes by 0-0.61 mm, with relative deviations of 0%-6.2%. And the deviation range between the calculated amplitudes and the set amplitudes were 0.16-0.94 mm, with relative deviations of 0%-6.3%. In the actual respiratory waveform motion mode, the deviation range between the calculated amplitudes and the set amplitudes were 0.10-0.48 mm, with relative deviations of 2.2%-8.6%.Conclusion:TrueFISP cine sequences show minimal deviations in determining the range of tumor head-foot motion and effectively captures the tumor's movement state, thereby providing important support for the precise definition of the tumor movement target area during radiotherapy .