Objective To observe the clinical efficacy of orthodontic treatment for displacement of anterior tooth caused by periodontal disease. Methods Forty-seven patients with displacement of anterior tooth caused by periodontal disease were divided into study group (26 cases) and control group (21 cases)according to the therapy method, the study group received periodontal basic treatment combined with orthodontic treatment, while the control group only received periodontal basic treatment, the changes of periodontal pocket depth, bleeding index, anterior coverage, overbite, alveolar bone height in two groups before and after treatment were observed and compared. Results The periodontal pocket depth, bleeding index, anterior coverage and overbite of study group after treatment were (3.12±0.53) mm, (27.30±4.25)%, (2.53 ± 0.76) mm and (2.18 ± 0.53 ) mm respectively and which were significantly lower than those before treatment [(5.35 ± 0.68 ) mm, (97.60±7.53 )%, (6.22±1.43 ) mm, (4.53±2.25)mm], the difference was statistically significant (P＜ 0.05 ), and the decrease extent was more obvious than the control group, the difference between two groups was statistically significant(P＜0.05); the above indicators in control group after treatment also dropped, but compared with those before treatment there was no significant difference (P ＞0.05); the alveolar bone height in both groups had no obvious change before and after treatment.Conclusion Periodontal basic treatment combined with orthodontic treatment can correct the malposed anterior teeth, improve the periodontal conditions, it has good application prospects.

Inwardly rectifying potassium (Kir) channels exhibit an asymmetrical conductance at hyperpolarization (high conductance) compared to depolarization (low conductance). The KCNJ10 gene which encodes an inwardly rectifying K+ channel Kir4.1 subunit plays an essential role in the inner ear and hearing. Mutations or deficiency of KCNJ10 can cause hearing loss with epilepsy, ataxia, sensorineural deafness, and renal tubulopathy (EAST) or SeSAME (seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance) syndromes. In this review, we mainly focus on the expression and function of Kir4.1 channels in the inner ear and mutation-induced EAST/SeSAME syndromes to provide insight for understanding the pathogenesis of deafness induced by KCNJ10 deficiency.
Deafness ; genetics ; metabolism ; Ear, Inner ; metabolism ; Hearing Loss, Sensorineural ; genetics ; metabolism ; Humans ; Intellectual Disability ; genetics ; metabolism ; Mutation ; Potassium Channels, Inwardly Rectifying ; genetics ; metabolism ; Seizures ; genetics ; metabolism

Leptin may indirectly suppress bone formation via a central nervous relay and may stimulate bone formation via direct peripheral effect. Leptin' s antiosteogenic function is mediated through the sympathetic nervous system and does not involve pathways with melanocortin and cocaime-amphetamine-regu-lated transcript, which are critical for leptin' s anorexigenic action. Leptin' s actions to modulate bone formation through sympathetic nervous system could suggest new therapeutic approaches for diseases such as osteoporosis. The progress of the relationship between leptin and sympathetic nervous system and bone formation is reviewed.

Objective To investigate the application of three dimensional blood vessel angiography(3D-CTA) in the patients of spontaneous subarachnoid hemorrhge diagonised as aneurysm.Methods 3D-CTA were examined in 32 cases of spontaneous subarachnoid hemorrhage.Results In 32 patients of spontaneous subarachnoid thirty patients were diagnosed as aneurysm.Two cases were not diagnosed.Conclusion 3D-CTA may be the first examination method in spontaneous subarchnoid hemorrhage.

Objective:To study the effect of 5-FU polylactic acid nanospheres(5-FU-PAN)on the proliferation and apoptosis of the human tongue squamous cell carcinoma Tca83 cells.Methods:Tca83 cells were exposed to 5-FU-PAN or 5-FU at the doses(mmol/L)of 1?10-2,2?10-2 and 4?10-2 for 1,3,5,7,9 and 11 days respectively.The survival rates of the cells were examined by MTT assay,the apoptosis of the cells was observed by acridine orange fluorescent staining and the cell cycle distribution was studied by flow cytometry.Results:Both 5-FU-PAN and 5-FU inhibited the proliferation and promoted the apoptosis of the cells dose and time dependantly.5-FU-PAN showed stronger effects than 5-FU.The two agents blocked the cells at S phase and 5-FU-PAN was more potential than 5-FU.Conclusions:5-FU-PAN is more effective in the growth inhibition and apoptosis promotion of Tca83 cells.

Objective To observe the electroretinogram (ERG) photopic negative response (PhNR) of idiopathic macular hole (IMH) in stage 2 by vitrectomy with or without internal limiting membrane peeling (ILMP).Methods Twenty-three stage 2 IMH patients (23 eyes) were enrolled in this prospective study.All patients received the best corrected visual acuity (BCVA),optical coherence tomography and flash-ERG examinations.The patients were randomly divided into group A (11 eyes,vitrectomy) and B (12 eyes,vitrectomy with ILMP).There was no significant difference in BCVA (t=0.96,P=0.350),diameter of macular hole (MH) (t=3.21,P=0.580) and the PhNR amplitude (t=0.98,P=0.353) in group A and B.All patients underwent 25G vitrectomy,ILMP was carried out in group B.The follow-up time was 3 to 6 months,with the mean follow-up time of 4.3 months.BCVA,MH closure rate and PhNR amplitude in group A and B were analyzed before and after surgery.Results Three months after surgery,10 eyes (90.9％) gained MH closure but 1 eye (9.1％) failed in group A.In group B,12 eyes (100.0％) gained MH closure.There was no significant difference in MH closure rate between the two groups (P=0.462).The mean BCVA of group A and B was 0.69 ± 0.24 and 0.65 ± 0.22,there was no significant difference between the two groups (t=0.49,P=0.722).The amplitude of PhNR in group A was (36.647.4) μtV,which was lower than the pre-surgery PhNR,but the difference was not significant (t=0.73,P=0.472).The amplitude of PhNR in group B was (27.1 ± 12.4) μV,which was lower than that the presurgery PhNR,and the difference was significant (t =3.56,P =0.002).The difference of PhNR amplitude in group A and B was statistically significant (t=2.17,P=0.042).Conclusion Compared with non-ILMP,vitrectomy combined with ILMP will significantly reduce the PhNR amplitude ofIMH in stage 2.

Objective The prestin,a motor protein responsible for outer hair cell(OHC)electromotility,is expressed on the OHC surface.Previous experiments revealed that OHC electromotility and its associated nonlinear capacitance was mainly located at the OHC lateral wall and was absent at the apical cuticular plate and the basal nucleus pole.Immunofluorescent staining for prestin failed to demonstrate the prestin expression at the OHC basal ends in whole-mount preparation of the organ of Corti.The aim of this study is to investigate the distribution of prestin at OHC.Methods In this experiment,the localization of prestin protein in single dissociated OHCs from cochlea of normal mouse,rat and guinea pig,were examined by immunofluorescent staining and confocal microscopy.Results We found that prestin was uniformly expressed on the OHC basolateral surface,including its basal pole.No staining was observed on the cuticular plate and stereocilia.The OHC lateral wall had a trilaminate organization and was composed of the plasma membrane,cortical lattice,and subsurface cisternae.By with co-staining with a membrane marker di-8-ANEPPS,prestin-labeling was found locating at the outer layer of the OHC lateral wall.Further separating the plasma membrane from the underlying subsurface cisternae,using a hypotonic extracellular solution,prestin-labeling was shown locating at the plasma membrane instead of the subsurface cisternae.Conclusion The data revealed that prestin is expressed in the plasma membrane on the whole OHC basolateral surface.

Objective:To assess the relationship between Insulin-like growth factor-I(IGF-I) and TCM syndrome of acute cerebral infarction by measuring IGF-I levels in patients of acute cerebral infarction.Methods:100 patients of acute cerebral infarction with 48 hours after onset were divided into three groups by TCM syndrome.The IGF-I levels in plasma were measured by saturation analysis of radioimmunoassay.Results:The IGF-I levels in plasma was(10.39?1.56)ng/ml in the group of wind-phlegm syndrome and stasis,(12.96?2.27)ng/ml in the group of wind-phlegm syndrome and exuberance of re,(13.54?3.22)ng/ml in the group of stirring of wind due to de ciency of yin.These values showed statistically signi cant di erence between groups.Conclusion:Acute cerebral infarction occured most frequently in the group of wind-phlegm syndrome and stasis,the IGF-I levels showed signi cant decrease in this group.

Objectives:B type ultrasonic diagnosis instrument is used to measure the sebum thickness and the relevance vs evaluated with sebum thickness calipers in sebum thickness determination. Methods:The study included 219 healthy adult persons and 11 cases of corpse dead of non disease reason for less than 10 hours.The measurements of sebum thickness were made by B type ultrasonic diagnosis instrument and sebum thickness calipers respectively. Results:The measurement results of two different methods were not significantly different. Conclusions:It is feasible to use B type ultrasonic diagnosis instrument for the measurement of the sebum thickness in clinic.

BACKGROUND: The injury of peripheral nerve may cause electroneurographic changes. However, what is the correlation between electroneurographic changes and the extent of nerve injury?OBJECTIVE: To probe into the changes of evoked electroneurography in early stage after nerve injury for clinical application and reference.DESIGN: Paired t-test in three groups of samples.SETTING: Department of Traumatic Orthopaedics People' s Hospital of Beijing University.MATERIALS:The trial was conducted in the Animal Center of the People's Hospital of Beijing University from August 2003 to December 2003. The subjects were 8 adult New Zealand rabbits of SPF grade(weighing 1.5 kg) and either gender from the Animal Center of Medical College of Beijing University.METHODS:The model of nerve injury was made in the 16 tibial nerves of the 8 rabbits, in which one third or two thirds of each nerve was ligated at the same transverse part. The samples were thus divided into three groups: non-injury (normal) group, 1/3 nerve injury group and 2/3 nerve injury group.MAIN OUTCOME MEASURES: The indices of evoked electroneurography, i. e. motor nerve condution velocity, negative M wave amplitude, negative M wave area, maximal M wave amplitude and maximal M wave area were measured in the three groups. The correlation between the extent of nerve injury and the 5 indices was analyzed.RESULTS: All the 5 indices of the 3 groups were significantly different( P＜ 0.05). The mean values of the 5 indices were 1.00 ±0.0,0.97 ±0.18,1.01 ±0.22;0.98 ±0.16 and 0.98 ±0.19 in normal group, 0.76 ±0.12,0.41 ± 0.24, 0.47 ± 0.30, 0.48 ± 0.27 and 0.50 ± 0.29 in 1/3 nerve injury group, and 0. 62±0. 10, 0. 11 ±0. 11, 0. 11 ±0. 10, 0. 12 ±0. 14 and 0.13± 0. 12 in 2/3 nerve injury group. The coefficient of correlation between the 5 indices and the injury were 0. 902, 0. 938, 0. 936, 0. 907, and 0. 914, respectively.CONCLUSION: In the early stage after peripheral nerve injury, the electroneurographic indices negatively correlate with the extent of nerve injury. The negative M wave amplitude and area are relatively more closely correlated with the extent of injury.