Objective To study the superiority and safety of cup-shaped womb manipulator auxiliary peritoneoscope in entire womb excision. Methods 180 cases with hysterectomy were randomly divided into cup-shaped womb manipulator auxiliary peritoneoscope group(treatment group) and tradition into the abdominal group( control group) ,and the amount of bleeding and postoperative complications,and patient recovery were observed in the two groups. Results Compared with the control group, the quality of life of patients with anal exhaust time, average length of staying, postoperative recovery daily time were significantly improved, and there were statistically significant differences between two groups ( P ＜ 0. 05 ). There was no serious complication in the two groups. Conclusion Cup-shaped womb manipulator auxiliary peritoneoscope hysterectomy was a safe and feasible operation,and could relieve harm and improve patients quality of life.

Objective To observe the efficacy of growth hormone ( GH) co-treatment in poor responders undergoing IVF-ET.Methods To analyze all the ovaries low response patients treated by IVF/ICSI,80 cases were ran-domly divided into two groups:40 patients in GH group were given GH along with Gn daily until the HCG administra-tion.40 patients in the control group received the same treatment protocol except the GH co -treatment.The catching spawn number and MⅡova,fertile rate,the number of perfect embryos ,pregnant rate were observed .The serum con-centrations of IGF-I,IGFBP-3 were detected by ELISA and compared between the two groups .Results The number of oocytes retrieved ,number of MII oocytes ,cleavage ,number of embryos available of GH group were increased without statistical significance(all P>0.05 ).The serum IGFBP-3 level of 2 groups had no statistical significance .The fertile rate,pregnant rate ,serum IGF-I level were significantly higher in GH group than those in the control group ( all P<0.05).Conclusion GH co-treatment in poor responders could improve the outcome of IVF-ET,which imply that GH could improve the quality of oocytes and embryos related to the elevated IGF-1 level in serum .

[Objective] To observe the clinical efficacy of treatment on Frequent Postoperative Posner-Schlossmann syndrome in cataract intraocular lens using Chinese medicine with the effect of tonifying the liver and kidney, nourishing yin and suppressing hyperactive yang.[Methods] 46 cases of Posner-Schlossmann syndrome were randomly divided into two groups, one was the Chinese Medicine group ,in which al cases were treated with Chinese herbs and local eyedrop at the time of first onset after operation; another was the control group, in which al cases were treated with local eyedrop. [Results] Within two years after treatment, compared with the control, in the Chinese Medicine group, the cases of recurrence of Posner-Schlossmann syndrome were obviously decreased. [Conclusion] Chinese medicine with the effect of tonifying the liver and kidney, nourishing yin and suppressing hyperactive yang shows definite effect on postoperative Posner-Schlossmann syndrome, it can decrease the occurrence frequency of number.

Objective To explore the relationship between the HBV‐DNA ,ALT ,AST and NO ,iNOS ,SOD levels in serum of patients with chronic hepatitis B(CHB) .Methods 24 patients with mild CHB ,30 patients with moderate CHB ,18 patients with se‐vere CHB and 30 healthy individuals were selected and set in group A ,B ,C and D ,respectively .The serum levels of HBV‐DNA , ALT ,AST ,NO ,iNOS ,and SOD were detected by FQ‐PCR and chemical analysis respectively .Results There were significant difference in the levels of ALT ,AST ,NO ,iNOS and SOD between group D and group A ,B and C (P<0 .05) .The serum level of ALT was positive relative to the levels of NO and iNOS(r=0 .487 ,0 .521 ,P<0 .05) ,and was negative relative to the level of SOD (r= -0 .574 ,P<0 .05) .The serum level of AST was positive relative to the levels of NO and iNOS(r=0 .453 ,0 .545 ,P<0 .05) , and was negative relative to the level of SOD(r= -0 .484 ,P<0 .05) .Conclusion With the increase of ALT and AST levels ,the levels of NO and iNOS increase ,and the level of SOD decreases simultaneously in CHB patients .It is suggested hepatocellular inju‐ry .

Objective To investigate the relationship among paraoxonase-1 (PON 1) activity in serum,PON 1 Q192R polymorphism and chronic renal failure disease ( CRF ) in the hans of Hunan.Methods PON 1 activity in serum and PON 1 Q192R polymorphism of 79 patients with CRF and 90 healthy subjects as control were measured.Results PON 1 activity in serum of patients with CRF (372 6?150 6)U was significantly lower than that in control group (537 8?172 2)U(P

The author analyses the chance and challenge of foreign periodicals booking in hospital libraries following China entering WTO and under the network environment,and proposes some effective solutions.

Objective To investigate G. Pentaphyllums influence on the activity of glutathione peroxidase (GSH-PX) and catalase (CAT) in the skin of hireless photoaging mice, as well as the content of hydroxyproline (HYP). At the same time, G. Pentaphyllum's effect on the hireless photoaging mice was studied. Methods Healthy KM hireless mice were randomly divided into five groups. A skin photoaging model was established by G. Pentaphyllum administration. The changes of the activity of GSH-PX, CAT and the contents of dermal hydroxyproline in the skin were then measured by biochemical methods in each group of the experiment. Results In the model group, the activity of GSHPX, CAT and the content of HYP of skin were all decreased significantly (P＜0. 05). And there were different degrees of improvements in those of the herb given groups. Conclusion G. Pentaphyllumhas the protective effect on the skin of the photoageing in the hairless mice. The mechanism may be that G. Pentaphyllum could inhibit the oxidative damages of ultraviolet and skin photoaging by increasing the synthesis of collagen in skin.

Objective To observe the effect and side effects of concurrent chemoradiotherapy for stage ⅡB～ⅢB cervical cancer. Methods 126 patients with stage ⅡB～ⅢB cervical cancer were randomly allocated into 2 groups. Concurrent chemoradiotherapy group: radiotherapy carried out same as the neoadjuvant chemotherapy group, i.e.firstly with cisplatin 20 mg iv d1-5, 5-Fu 750 mg iv d1-5, repeated every 28 days, total 4 cycle; the neoadjuvant chemotherapy group i.e.firstly with cisplatin 20 mg iv d1-5, 5-Fu 750 mg iv d1-5, repeated every 28 days, total 2 cycle, after chemotherapy received routine radiotherapy 2 Gy per day, 5 times a week to a total dose of 30 Gy with 192Ir brachytherapy, 7 Gy per week. When total dose reached 46 Gy, the middle field was shielded by plumbum, then continuous radiotherapy, total dose reach A point 65-70 Gy, B point 50-56 Gy. Results All patients were followed-up for more than five years. The follow-up rate was 94.4 %. In concurrent chemoradiotherapy group the 3 year survival rate and the 5 year survival rate were 82.8 %, 65.6 %, In neoadjuvant chemotherapy group the 3 year survival rate and the 5 year survival rate were 67.7 %, 46.8 %. There was a significant difference in two groups (X2=3.86, P＜0.05; X2=5.01, P＜0.05), no significant difference in toxicity-side effect. Conclusion Concurrent chemoradiotherapy for advanced cervical cancer can significantly improve the 3-year and 5-year survival rate and has little increase in toxicity-side effect.

Objective To observe the effect of silencing Snail gene on the invasion and proliferation ability of human pancreatic cancer cell line PANC1.Methods Lentiviral vectors that can express small hairpin RNA(shRNA) targeting human Snail gene(shRNA-Snail) or shRNA sequence that did not match any known mRNA(shRNA-NC) were constructed,and transfected into PANC1 cells.Untransfected cells served as control.mRNA and protein expression of Snail,α-smooth muscle actin (α-SMA) and E-cadherin was determined by real time quantitative PCR and Western blotting,respectively.In vitro invasion ability was tested by Transwell model.Proliferation ability was measured by CCK-8 assay.Results Compared with those in shRNA-NC group,Snail mRNA (0.27 ± 0.02 vs 0.92 ± 0.03) and protein level (0.26 ± 0.02 vs 0.80 ± 0.02),and α-SMA mRNA (0.33 ±0.04 vs 0.97 ±0.07) and protein level (0.31 ±0.04 vs 0.74 ±0.06) in shRNA-Snail group were obviously decreased,but E-cadherin mRNA (1.57 ± 0.45 vs 0.95 ± 0.08) and protein level (0.86 ± 0.03 vs 0.20 ± 0.03) were greatly increased.The number of cells permeating the septum of transwell [(6.80 ± 0.73)/400 magnification vs (26.80 ± 2.52)/400 magnification,P ＜0.01] was significantly decreased,and cell proliferation was inhibited (0.74 ± 0.05 vs 1.47 ± 0.04,P ＜ 0.01).All the differences above were statistically significant (all P ＜ 0.01).No significant differences were observed between shRNA-NC and normal control group.Conclusions Silencing Snail gene may restrain the invasion and proliferation ability of PANC1 cells to a certain degree.

In order to research into the relationship between atrial natriuretic peptide (ANP), aldosterone (Aldo) and pregnancy-induced hypertension (PIH), as well as normal pregnancy, the plasma concentrations of ANP and Aldo from normal pregnant women. PIH patients and their fetuses were measured by radioimmunoassay. It was found that maternal venous Aldo and ANP were higher in normal pregnant women than those in non-pregnant women. The plasma ANP was higher in PIH patients than that in normal pregnant women while plasma Aldo was lower. The plasma concentration of ANP was higher in the fetuses of PIH patients than that in the normal controls, but Aldo was lower. It is concluded that ANP concentration correlates significantly to the severity of PIH, and may be used for an early diagnosis of PIH.