Objective To investigate the clinical value of remote electronic fetal monitoring network in intrahepatic cholestasis of pregnancy(ICP). Methods Non-stress test (NST) was carried on in 54 cases of ICP by remote electronic fetal monitoring network(study group). Fifty-four cases of ICP served as control group were monitored by fetal movement counting daily and regular NST check-up in hospital. Abnormal NST and perinatal outcome were compared between the study group and control group. Results The incidence of abnormal NST was significantly higher in the study group than that in the control group ( 36.1% vs 27.2%, P 0.05). Conclusion Remote fetal monitoring network can be used to improve perinatal outcome of ICP. It is a new method of FHR self-monitoring for ICP case.

Aim To study effects of morphine tolerance and dependence on the fast excitatory synaptic transmission in sympathetic ganglia of rats.Methods The isolated sympathetic ganglia,superior cervical ganglia(SCG), were made from control and morphine tolerant and dependent rats respectively.Effects of morphine tolerance and dependence on the fast excitatory synaptic transmission in rat sympathetic ganglia were studied by means of intracellular recording technique.Results ① Morphine(0.1～1.0 mmol?L~(-1))reversibly inhibited the amplitude of the fast excitatory postsynaptic potentials(f-EPSPs) in SCG neurons of control rats.② Compared with control group,inhibitory effects of morphine(0.5 mmol?L~(-1) and 1.0 mmol?L~(-1)) on f-EPSPs in SCG neurons of morphine tolerant and dependent rats were obviously decreased;③ Naloxone(0.1 mmol?L~(-1)),which had no significantly effect on f-EPSPs in SCG neurons of control rats,could reversibly facilitate the amplitude of f-EPSPs in SCG neurons of morphine tolerant and dependent rats;④ No significant difference of RMP and Rm was founded between SCG neurons of control and morphine tolerant and dependent rats.Conclusion The morphine tolerant and dependent of the fast excitatory synaptic transmission in rat sympathetic ganglia has been formed in morphine tolerant and dependent rats.

Aim To study the effects of chronic morphine treatment on the contents of cAMP and cGMP in sympathetic ganglia,superior cervical ganglia(SCG) of rats.Methods The chronic morphine dependent model of rats was established by subcutaneous injection of morphine in gradually increasing doses for 5 days,the dependence and the tolerance of the model was estimated by naloxone precipitation test and 55℃ tail-flick trail test respectively.The contents of cAMP and cGMP in SCG were detected by means of 125I radioimmunoassay.Results ① Compared with control group,the content of cAMP in SCG of morphine-acute group rats was descended(P0.05;compared with morphine-acute,P0.05).Conclusion There was an up-regulation of cAMP in sympathetic ganglia of chronic morphine treated rats.

Objective:to study cognitive impairment of patients with Alzheimer disease (AD). Method:62 patients with AD and 159 normal elderly were tested with MMSE. Result:The patients group had lower scores in all subtests except that of naming. Level of education had positive correlation with the total score, subscores of attention and calculation, understanding, and describe graph. Conclusion:MMSE can be used in clinical assessment of cognitive function of patients with AD.

Objective To study changes of dielectric properties of gastrocnemius caused by simulated weightlessness.Methods Male Sprague-Dawley rats tail suspended for 10 wk were observed together with its normal control.AC impedance of isolated gastrocnemius was measured with an impedance analyzer(Agilent 4294A).Effects of simulated weightlessness on electric properties of cells in isolated rat sural muscle were also observed by analyzing the dielectric numerical characters of the dielectric spectra,the Cole-Cole plots,spectrum of loss factor and loss tangent,as well as spectrum of conductivity imaginary part.Results It was found that simulated weightlessness could induce certain changes:both relative permittivity at low frequency ?L and relative permittivity increment ??,as well as conductivity at high frequency ?h and conductivity increment ?? were all reduced.The first characteristic frequency fC1 decreased,while the second characteristic frequency fC2 increased.Peak of loss factor,peak of loss tangent and the maximum of conductivity imaginary part were also reduced.Conclusion Changes of dielectric property of gastrocnemius induced by simulated weightlessness can be observed effectively by frequency domain impedance technique.

ObjectiveTo explore the efficacy and side-effects of reboxitine on post-schizophrenic depression.Methods30 patients with post-schizophrenic depression according CCMD-Ⅲ accepted reboxitine 4～8 mg/d in addition to antipsychotic drugs for 6 weeks. Efficacy and side-effects were determined with Hamilton Depression Scale (HAMD), Brief Psychiatric Rating Scale (BPRS), Scale for Assessment of Negative Symptoms (SANS), Treatment Emergent Symptom Scale (TESS) before and 2, 4, 6 weeks after treatment. ResultsThe score of HAMD were obviously improved after the treatment(P<0.01), as well as that of negative psychotic symptoms in BPRS and TESS. Slight symptoms that may be associated to the Reboxitine were obvioused seldom, and all recovered without any treating. ConclusionLow-dose rebositine is effective on post-schizophrenic depression with few side-effects and good compliance.

AIM: To explore the mechanisms of resistance to 5-fluorouracil(5-FU) in human colon cancer cells.METHODS: 5-FU-resistant cell lines were established and their IC50 were calculated by detection of cell survival rate.Western blotting was performed to analyze the expression of several proteins,by which the possible mechanisms of acquired resistance to 5-FU were determined in human colon cancer cells.RESULTS: The resistant cells were resistant to 5-FU-induced S phase arrest as well as the expression of DNA damage marker-phosphor-histone H2A.X.Furthermore,data demonstrated that over-expression of Bik,Bcl-Xs,and Bcl-XL proteins were observed in 5-FU-resistant cell lines.However,the DLD1/Bcl-XL cells were only partially resistant to 5-FU-induced apoptosis,but not 5-FU-induced S phase arrest and phosphor-histone H2A.X.CONCLUSION: Over-expression of Bcl-XL protein certainly contributes to acquired 5-FU resistance in human colon caners,but has no effect on 5-FU-induced DNA damage and cell cycle disorder,suggesting that other mechanisms are involved in acquired resistance to 5-FU in human colon cancer.

Objective To compare the different types of physical artificial liver support system(ALSS) applied in the treatment of patients with early stage of chronic severe hepatitis in order to evaluate the therapeutic effect and look for the most effective one.Methods One hundred and twenty patients of chronic severe hepatitis B in early stage were randomly divided into 4 groups(each n=30).The patients in group 1 were treated by 3 000 ml plasma exchange;in group 2,by 2 000 ml plasma exchange combined with plasma adsorption for 2 hours;in group 3,only by plasma adsorption for 3 hours and in group 4,by molecular adsorbent re-circulating system(MARS).The observation time lasted for 2 weeks and the therapy twice a week,the interval between two times of treatment being 2-3 days.The improvement of symptoms,the fade away and rebound of icterus,the changes of albumin and prothrombin activity(PTA),side effects(anaphylaxis,hepatic encephalopathy,edema of conjunctiva,burp,fluid and electrolyte balance failure,changes of blood routine test and ammonia level,etc) were compared before and after the treatment.Results The symptoms of 27 cases in group 1 were improved,the rate of decrease of total bilirubin(TBil) of group 1 was 51.8% and the range of icterus rebound was 14.4% to 46.7% with comparatively more side effects.The symptoms of 24 cases of group 2 were improved,the rate of decrease of TBil in group 2 was 62.2% and the range of icterus rebound was 2.8% to 28.5% with comparatively less side effects.The symptoms of 16 cases of group 3 were improved,the rate of decrease of TBil in group 3 was 38.8% and the range of icterus rebound was 2.8% to 6.8% with less side effects.The symptoms of 26 cases of group 4 were improved,the rate of decrease of TBil in group 4 was 60.1% and the range of icterus rebound was 7.8% to 27.4% with comparatively more side effects,moreover the cost was higher than that in the other groups.Conclusion The method of reduced plasma exchange combined with plasma adsorption with macroporous resin used to cure chronic severe hepatitis at early stage has the characteristic of good effects for the fade away of icterus,little rebound of icterus and side effects.It is the reasonable method of artificial liver to cure the early stage of chronic severe hepatitis.

Homoacetogens are a group of microorganisms with application potential to produce chemicals and biofuels by the bioconversion of synthesis gas. In this study, we collected waste activated sludge samples to screen homoacetogens by Hungate anaerobic technique, and studied the effect of pH on acetate and alcohol production from H2/CO2 gas. The mixed culture contained Clostridium ljungdahlii, Lysinibacillus fusiformis and Bacillus cereus. Acetate concentration achieved 31.69 mmol/L when the initial pH was 7. The mixed culture containing homoacetogen could converting H2/CO2 to acetate, which provides an efficient microbial resource for the bioconversion of synthesis gas.
Acetates ; chemistry ; Bacteria ; classification ; Biofuels ; Carbon Dioxide ; Hydrogen ; Sewage ; microbiology

Objective The aim of the study is to discuss the application value of HA330-Ⅱ microporous resin plasma adsorption in liver support and the treatment of complications of chronic severe hepatitis.Methods The treatment group has 41 patients of chronic severe hepatitis B,which were treated for 3 times by HA330-Ⅱ microporous resin plasma adsorption.We compared the improvement of symptom,the fadeamay and rebound of icterus,the change of hepatic encepllalOpathy and brain oedema,the change of blood routine test,blood ammonia level,blood-gas analyzing result,cruor index,inflammatory factors(IL-1b,IL-6,IL-8,TNF-α,etc.),side effects,etc.The control group has 37 patients of the same type.Results HA330-Ⅱ microporous resin plasma adsorptioin is partly effective for the improvement of symptom,improvernent rate 43.9%,and without new discomfort due to the treatment. It obtains some extent fadeaway of icterus which has a low rebound,reduce TBiL(17.83±3.65)% and there be no statistics difference of rebound.It lowers the blood ammonia level and improves the hepatic encephalopathy and brain oedema.It absorbs cytokine IL-8.Atthough it has some extent effect to albunfin,blood paltelet,haemoglobin.Conclusion HA330-Ⅱ microporous resin plasma adsorption has good application value in the treatment of chronic severe hepatitis B.It is a new treatment choice of severe hepatitis.