1.Development of internet-based home tele-monitoring system for fetus
Chinese Medical Equipment Journal 1989;0(02):-
The internet -based home tele -monitoring system for fetus has the functions of monitoring fetal heart and movement, analysis and alarm. With this system, data related to fetuses can be shared and real -time analyses and suggestions from doctors can be available. Current network system is involved in, and thus it's inexpensive and practical.
2.Determination of Residues of Organochlorinated Pesticides in Polygonum multiflorum by GC-MS with Solid-phase Extraction
China Pharmacy 2005;0(19):-
OBJECTIVE:To determine the residues of 9 kinds of organochlorinated pesticides in Polygonum multiflorum.ME-THODS:Samples were extracted with ethyl acetate by ultrasonic wave assistant extraction and purified on Florisil solid-phase extraction column.The residues of organochlorinated pesticides were determined with capillary gas chromatography with GC-MS.The separation was performed on DB-5MS(30 m?0.25 mm,0.25 ?m) fused-silica capillary column with injector temperature of 230 ℃.The initial column temperature was 100 ℃,then raised to 220 ℃ at the rate of 8 ℃?min-1.The column temperature was ra-ised at 250 ℃ at the rate of 5 ℃?min-1 for 10 min.The column flow was 1 mL?min-1 and injection volume was 1 ?L.RESULTS:9 kinds of organochlorinated pesticides were completely separated within 30 minutes.The average recoveries ranged from 80.4% to 97.2%(RSD ranged from 3.5% to 7.4%,n=6).CONCLUSION:The method is sensitive and accurate for the content determination of the residues organochlorinated pesticides.
3.Progress on stent graft induced new entry after Thoracic endovascular aortic repair
Hongbo CI ; Qingbo FANG ; Xiaohu GE
International Journal of Surgery 2015;42(12):838-841
Thoracic endovascular aortic repair (TEVAR) has been increasingly used in the treatment of Stanford type B dissection.The incidence of new entry after thoracic endovascular aortic repair has been gradually increased report including at the proximal end and at the distal end of the endograft.New entry is difficult to handle following thoracic endovascular aortic repair for aortic dissection,and associates with a high substantial mortality.It need pay more attention to prevention and treatment on new entry after thoracic endovascular aortic repair.We summary and analyze the possible causes,prevention and management of new entry after thoracic endovascular aortic repair for aortic dissection.This article review and conclusion the progress on stent graft induced new entry after thoracic endovascular aortic repair.
4.The effect of growth hormone in poor responders undergoing COH
Fang NIU ; Liuming LI ; Hongbo WU
Chinese Journal of Primary Medicine and Pharmacy 2014;(19):2902-2904
Objective To observe the efficacy of growth hormone ( GH) co-treatment in poor responders undergoing IVF-ET.Methods To analyze all the ovaries low response patients treated by IVF/ICSI,80 cases were ran-domly divided into two groups:40 patients in GH group were given GH along with Gn daily until the HCG administra-tion.40 patients in the control group received the same treatment protocol except the GH co -treatment.The catching spawn number and MⅡova,fertile rate,the number of perfect embryos ,pregnant rate were observed .The serum con-centrations of IGF-I,IGFBP-3 were detected by ELISA and compared between the two groups .Results The number of oocytes retrieved ,number of MII oocytes ,cleavage ,number of embryos available of GH group were increased without statistical significance(all P>0.05 ).The serum IGFBP-3 level of 2 groups had no statistical significance .The fertile rate,pregnant rate ,serum IGF-I level were significantly higher in GH group than those in the control group ( all P<0.05).Conclusion GH co-treatment in poor responders could improve the outcome of IVF-ET,which imply that GH could improve the quality of oocytes and embryos related to the elevated IGF-1 level in serum .
5.Catheter-directed thrombolysis versus anticoagulant alone for treatment of deep venous thrombosis: a Meta-analysis of randomized trials
Sheng GUAN ; Qingbo FANG ; Hongbo CI ; Xiaohu GE
International Journal of Surgery 2015;42(12):803-807
Objective To evaluate the efficacy and safety of catheter-directed thrombolysis (CDT) combined with anticoagulant compared with traditional treatment (Anticoagulant alone A C) for deep venous thrombosis.Methods We searched Medline,Embase,Cochrane Central Register of Controlled Trials,PubMed,Chinese Biomedical Literature Data Base (CBM),Chinese Scientific Journal,Full-text Data Base (CSJD),and,added with hand searcing and other retrievals.The Cochrane Collaboration's RevMan 5.0.18 was used for Meta-analysis.Results Four randomized controlled trials were available and were included in the study.Meta-analysis showed that 6 months after treatment,iliofemoral vein patency rate of CDT group was higher than that of AC group (OR =5.13,95% CI:2.01-13.14,P =0.0006);Major complications of CDT group compared to those of AC group were not statistically significant(OR =2.74,95% CI:0.76-8.07,P =0.13),but the minor complications and total complications of CDT group were higher than those of AC group [(OR =7.86,95% CI:3.10-19.90,P <0.0001)and(OR=5.42,95%CI:1.47-20.01,P=0.01)].Conclusions CDT is a positively effective way to treat early DVT.Application of CDT in patients without contraindications to its use can have good therapeuic effect.
6.Preliminary clinical study on distal-end tear of Stanford type B aortic dissection
Yufeng XIAO ; Qingbo FANG ; Bing ZHU ; Hongbo CI ; Xiaohu GE
International Journal of Surgery 2016;43(3):178-181
Objective The objective of this article is to attempt to propose the endovascular repair principles of distal-end tear of Stanford type B aortic dissection.Methods The vascular surgery of xinjiang uygur autonomous region people's hospital received and cured 101 patients of Stanford B aortic dissection from January 2013 to January 2015.The patients are divided into two groups according different treatment principles:(1)There are 57 cases in sequential treatment group,performing endovascular repair of aortic tears from near to far,(if the tear at visceral artery is not treated then the distal-end tear is also not treated);(2) There are 44 cases in non-sequential treatment group,not performing endovascular repair of aortic tears from near to far (the tears involving visceral artery are not treated and the remaining distal-end tears are performed endovascular repair).After operation,carry out statistical analysis between two groups on the growth rate of aortic diameter of the coeliac axis,occurrence rate of main discomfort complaint,false lumen thrombosis rates.Results After operation,between the two groups,the growth rate of aortic diameter of the coeliac axis is obvious difference(P < 0.05),that the sequential group is with a low rate;there are obvious differences on the occurrence rates of main discomfort complaint and false lumen thrombosis rates (P < 0.05),that the sequential group is superior to the non-sequential group.Conclusions After a preliminary clinical study,we get a conclusion that when treating distal-end tears of Stanford type B aortic dissection,sequential treatment is better than non-sequential treatment.
7.Twelve cases report of the vagus splenic aneurysm and literature review
Qingbo FANG ; Hongbo CI ; Yufeng XIAO ; Sheng GUAN ; Xiaohu GE
International Journal of Surgery 2015;42(3):180-182
Objective To assess the treatment of splenic artery aneurysms(SAA) and curative effect evaluation.Methods Twelve SAA patients treated in our hospital from January 2012 to May 2014 were clinical analyzed.The male in Twelve patients was 4 man and others were female.The vagus splenic artery aneurysms are originated from the superior mesenteric artery,tumors are single,from 1.5cm to 2.8cm in diameter,an average of 2.1cm.Twelve cases were performed surgery,4 patients underwent elective surgery,interventional embolization of the splenic aneurysm in 3 patient,The others were performed interventional embolization + superior mesenteric artery covered stents.Results Technical success was achieved in all twelve patients,2 patients had adverse effects such as abdominal pain,fever,etc.There revealed no aneurysm recurrence was found.Twelve patients were followed for 6-24 months,the follow-up by examinations with electronic computer X-ray tomography or color Doppler ultrasonic as well as angiography every 3 months.One patient died of severe abdominal bleeding 1 year later after the operation and the other eleven patients remained in good condition with no occurrence of re-canalization of the lesions.Conclusions For the vagus splenic aneurysm with suitable for anatornic conditions,cavity therapy is safe and effective,for the vagus splenic aneurysm involving hepatic artery,need to open surgery for vascular remodeling.
8.Perioperative safety of minimally invasive and open pancreaticoduodenectomy: a Meta analysis
Purun LEI ; Jiafeng FANG ; Bo WEI ; Hongbo WEI
Chinese Journal of Digestive Surgery 2014;13(9):694-701
Objective To evaluate the perioperative safety of minimally invasive and open pancreaticoduodenectomy (PD).Methods The China National Knowledge Infrastructure,VIP,PubMed,The Cochrane Library and EMBASE were searched with the key words of laparoscopic,laparoscopy,robotic,da Vinci,minimally invasive,pancreaticoduodenectomy,微创,腹腔境,机器人,胰十二指肠切除术 to retrieve literatures on minimally invasive and open pancreaticoduodenectomy.All the patients were divided into the minimally invasive PD group and the open PD group,and the patients in the minimally invasive PD group were further divided into the robotic PD group and the laparoscopic PD group.A meta analysis was carried out using the RevMan 5.2 software.The count data were represented by the odds ratio (OR) and 95% confidence interval (95% CI),and the measurement data were represented by the weighted mean difference (WMD) and 95 % CI.Heterogeneity of the publication was analyzed using chi-square test,and the publication bias was analyzed using the funnel plots.The stability of results was analyzed using the sensitivity analysis.Results Ten literatures including 690 patients were selected.There were 235 patients in the minimally invasive PD group,and 455 in the open PD group; 107 patients were in the robotic PD group and 128 in the laparoscopic PD group.There were no significant difference in the incidence of postoperative complications,pancreatic fistula,delayed gastric emptying,postoperative hemorrhage,bile leakage,reoperation,mortality and number of lymph nodes dissected between the minimally invasive PD group and the open PD group (OR =0.75,1.01,0.98,1.50,0.94,0.56,1.06,WMD =2.29,95% CI:0.40-1.41,0.67-1.53,0.55-1.77,0.76-2.94,0.37-2.38,0.26-1.20,0.48-2.32,-0.55-5.13,P > 0.05).Although the operation time of the minimally invasive PD group was significantly longer than the open PD group,lesser volume of blood loss,shorter duration of postoperative hospital stay and higher R0 resection rate was detected in the minimally invasive PD group (WMD =99.57,-355.70,-3.30,OR =0.49,95% CI:36.99-162.15,-608.38--103.01,-6.58-0.03,0.26-0.92,P < 0.05).The operation time of the laparoscopic PD group was significantly longer than that of the open PD group (WMD =93.17,95% CI:55.98-130.37,P < 0.05).The operation time of the robotic PD group was comparable to that of the open PD group (WMD =122.96,95% CI:-48.48-294.40,P > 0.05).There was no significant difference in the duration of postoperative hospital stay between the laparoscopic PD group and the open PD group (WMD =-0.81,95% CI:-5.08-3.45,P > 0.05).The operation time of the robotic PD group was significantly shorter than that of the open PD group (WMD=-6.82,95%CI:-13.21--0.44,P<0.05).Conclusions The perioperative safety of minimally invasive PD was comparable to open PD.Minimally invasive PD has the advantages of lesser blood loss and quick recovery of patients.Robotic PD could significantly decrease the operation time.Minimally invasive PD could be a reasonable alternative when the requirement of indications is fulfilled.
9.The misunderstanding and comprehension of hybrid operation for treating aortic dissection involving aortic arch
Hao REN ; Hongbo CI ; Sheng GUAN ; Qingbo FANG ; Xiaohu GE
Journal of Chinese Physician 2014;16(3):315-318
Objective To explore the misunderstanding and comprehension of hybrid operation for treating aortic dissection involving aortic arch.Methods From March 2009 to November 2013,13 patients received hybrid operation for aortic dissection involving aortic arch in the People's Hospital of Xinjiang Urgur Autonomous Region were enrolled,including male 11 and female 2,and aged 36 ~ 60 years old with a mean age (44 ± 6.8) years old.All patients were type-B aortic dissection.All of them were not suitable to be treated with endovascular exclusion monotherapy.The ascending aorta-brachiocephalic artery bypass and left carotid artery bypass was established with median sternotomy approach and neck incision in 13 patients,and 2 patients did left subclavian artery bypass additionally,then retrograde endovascular stent graft implantation was used.Computed tomography angiography (CTA) scanning at 3-month,9-month,1-year and every-year after operation showed no stent grafts translocation and bypass graft obstruction.Results The surgical operation and stent grafts implantation were completely successful.Angiography showed 1 case had end leakage and other cases no obvious displacement or end leakage of stent grafts in operation.Blood flow in true lumen of aortic dissection was recovered and all of bypass grafts were unobstructed.No death and severe complications occurred.All patients were followed-up with 3 to 56 months [(29.0 ± 10.2) months],and all patients resumed normal life.Enhanced CT scanning after operation showed 1 case had endoleak and other cases no endoleak,stent grafts translocation and bypass graft obstmction.No signs of brain and limb ischemia were observed.Conclusions To summarize misunderstanding and experience by continuous explore feature of hybrid operation for treating disease involving aortic arch,we developed a more reasonable surgical treatment options that can improve the success rate of complex aortic dissection surgery,and ultimately achieve better surgical results.
10.Directional differentiation of bone marrow mesenchymal stem cells into oligodendrocytes induced by the combination of various neurotrophic factors
Yongtao XU ; Feng LI ; Tie LIU ; Hongbo YOU ; Zhong FANG
Chinese Journal of Tissue Engineering Research 2009;13(14):2793-2796
BACKGROUND: Myelination following axonal regeneration is a key factor affecting the recovery of spinal cord injury. Oligodendrocyte survival directly affects the myelination following axonal regeneration. OBJECTIVE: To investigate the feasibility of differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into oligodendrocytes induced by neurotrophic factors. DESIGN, TIME AND SETTING: The cell molecular biology in vitro study was performed at the Laboratory of Department of Orthopaedics, Tongji Hospital from September 2006 to June 2007. MATERIALS: A total of 5 Sprague Dawley rats aged 2-4 weeks, of both gender were selected. Bilateral femur and tibia bone marrow was obtained to harvest BMSCs. METHODS: At passage 4, BMSCs were incubated in serum-free medium, supplemented with N2, 20 ng/mL basic fibroblast growth factor, 20 ng/mL epidermal growth factor for 48 hours, and incubated in medium containing 500 ng/mL insulin-like growth factor I and N2 for 3 days. MAIN OUTCOME MEASURES: Morphological changes were observed using an phase contrast microscope. Semiquantitative RT-PCR was utilized to detect specific marker mRNA expression of oligodendrocytes. Using neuron marker anti-microtubule-associated protein, astrocyte marker anti-glial fibrillary acidic protein, oligodendrocyte marker anti-galactocerebroside, anti-myelin basic protein antibody, immunocytochemical staining was performed to detect the positive rate of the differentiation of BMSCs into oligodendrocytes. RESULTS: Morphological changes in BMSCs during the differentiation into oligodendrocytes: After the induction, a majority of BMSCs presented the morphological characteristics of oligodendrocytes. Cytoplasm retraction towards nucleus, cell process extension towards outwards, and strong refraction were found. With the prolongation of time, several cell processes connected and formed a typical net-shape structure. Specific marker mRNA expression of oligodendrocytes: Following induction, specific strap of myelin basic protein mRNA and galactocerebroside mRNA could be detected. Positive rate of oligodendrocytes: During induction, the positive rates of galactocarebroside, myelin basic protein and microtubule-associated protein were 65%, 45% and 10%, respectively. CONCLUSION: The combination of epidermal growth factor, basic fibroblast growth factor and insulin-like growth factor can effectively promote the directional differentiation of BMSCs into oligodendrocytes.