Wildtype p53-induced phosphatase 1 (Wip1) is a serine/threonine protein phosphatase of 605 ami-no acids, which is expressed at high levels in many organs and tissues .As Wip1 is overexpressed in human tumors , analy-sis of Wip1 has focused primarily on its role in tumorigenesis .In recent years , it has also been shown that Wip 1 plays an important role in several physiological processes including adult neurogenesis , senescence , immunodeficiency and metabolic diseases.This review addresses how Wip1 participates in physiological and pathological conditions at cellular and molecular levels.

Cardiomyopathies ; Crystallins ; Humans

Objective To examine current use, associated complications, and short-term outcomes in patients with acute myocardial infarction (AMI) requiring intra-aortic balloon pump (IABP) counterpulsation. Methods A single-centre registry was conducted between October 2004 and July 2008. Data were collected from 121 consecutive AMI patients treated with IABP counterpulsation at the 28th Division of Beijing Anzhen Hospital. Results Among 806 patients with AMI, IABP counterpulsation was employed in 121 patients. Placement of an IABP was indicated for hemodynamic support during high-risk catheterization and/or angioplasty (66.9%) and cardiogenic shock (27.3%). Balloon insertions were successful in 98.3% of patients and duration of mean use was 34.2?16.2 hours. No major IABP complications occurred in all the patients. Diagnostic catheterization was performed in all the patients inserted with IABP, and 93.4% underwent coronary revascularization before hospital discharge. The total in-hospital mortality was 13.2% (34.4% in patients with cardiogenic shock) and varied markedly by indication and use of revascularization procedures. Conclusion IABP counterpulsation may be successfully employed for a wide variety of conditions in the AMI setting, providing significant hemodynamic support with rare major complications in a high-risk patient population. IABP counterpulsation may reduce mortality in patients with AMI undergoing reperfusion therapy.

Objective To evaluate the sensitivity,specificity and accuracy of the algorithm for electrocardiographic identification of the IRA and exact site of occlusion of the IRA.Methods According to the algorithm for electrocardiographic identification,we predicted the IRA by analyzing ECG in 896 patients with ST-segment elevation myocardial infarction(STEMI),who were enrolled in this study and underwent primary coronary angiography.Results The results indicated that left main coronary disease showed a sensitivity of 100%,specificity of 99% and accuracy of 99%;and at the same time the left anterior descending artery(LAD) with 99%,99% and 99%(proximal LAD: 84%,90% and 88%;middle and distal LAD: 57%,94% and 88%);the left circumflex artery with 64%,95% and 91%;the right coronary artery(RCA):89%,92% and 91%(proximal RCA: 51%,96% and 88%;middle and distal RCA: 68%,92% and 85%) respectively.Conclusion This study suggests that electrocardiogram is a crucial tool in the identification of the IRA in left main coronary,LAD and proximal RCA with predicting exact site of culprit lesion.The LCX or middle and distal RCA can be difficult to discriminate.

Objective To study the synergistic antitumor effect of PCF when combine used with CTX.Methods Inoculated 0.2 mL H22 tumor fluid into the right armpit of mouse,and then the animals were divided into seven groups randomly: control group;CTX group(CTX 10 mg?kg-1);three test group(PCF 1000,500 and 250 mg?kg-1) and two combined use groups (PCF 1000,500 mg?kg-1with CTX 10 mg?kg-1).PCF were given by intragastric administration for 10 days,and CTX by intraperitoneal injection for 10 days.Same volume of saline was given to the control group.The mice were killed 24 hours after the last medication and the tumor inhibition rate was calculated.Results Three PCF groups had no effect on tumor inhibition,while two combined treatusement groups showed significant effect on tumor inhibition,while at the same doses the tumor inhibition rates were raised to 55.9% and 52.9% respectively,which were higher than that of CTX when used alone.Conclusion PCF can enhance the antitumor effects of CTX.

Objective To analyze retrospectively long term follow up of inferior vena cava filters in conjunction with thrombolysis and anti coagulant therapy in prevention and treatment of pulmonary embolism (PE) in 24 patients with deep vein thrombosis (DVT) Methods This study included 13 males and 11 females with an average age of 52 4 years (14-86 years) Percutaneous implantation of inferior vena cava filters was performed via a femoral vein in 24 patients with acute or subacute DVT, of whom two were given conjunctive catheter based urokinase thrombolysis After the procedure, 20 patients underwent intravenous urokinase thrombolysis with subcutaneous low weight molecular heparin for 10 days and subsequent oral warfarin for six months Results All patients underwent a successful interventional procedure with an average 15 month (10-48 months) follow up One week after the procedure, relief of symptoms related to DVT was achieved in all the 24 patients Neither filter migration and thrombolic occlusion of filter nor PE and major hemorrhage were observed in this series Conclusion The use of inferior vena cava filters in conjunction with thrombolysis and anti coagulant therapy is a safe and effective treatment modality in patients with DVT, which can be used to prevent subsequent PE

Objective To analyse frequency, risk factors and prognosis of stent thrombosis after primary percutaneous coronary intervention (PCI) for ST-segment elevation myocardial infarction (STEMI) during the last five-year period. Methods Primary PCI was performed in 311 patients with STEMI including 250 males and 61 females with an average age of 60.4 years (26～85 years). The standard triple antiplatelet/anticoagulation therapy with aspirin and clopidogreal plus heparin was used pre- and post-interventional procedure. All patients underwent more than two months follow-up investigation. Results A successful primary PCI procedure was achieved in 99% (308/311) STEMI patients with incidence of total stent thrombosis in 2.6% (8/308), including acute stent thrombosis in five patients and subacute or late thrombosis in three patients. Multiple risk factors could be identified in all the eight patients, of whom, three died of acute closure events and the follow-up study in the other five showed severe systolic dysfunction of left ventricle. Conclusion High incidence of stent thrombosis occurred after primary PCI for STEMI with high mortality and poor clinical prognosis. Application of small diameter and/or long stents and discontinuation of clopidogreal seem to be major risk factors.

Objective To describe our follow-up results of interventional management in 20 patients with subclavian artery stenosis. Methods This study involved in 20 symptomatic patients with an average age of 63.4 years,a mean stenose severity of 86.2% and a mean systolic arterial pressure difference of 116 mm?Hg between both upper limbs. Percutaneous transluminal angioplasty (PTA) in the first 8 patients and stenting in the last 12 patients were performed via a right femoral or radial artery. Results Both PTA and stenting were successfully archived in all patients with normalization of radial pulses and a mean systolic arterial pressure difference of 24 mm?Hg between both upper limbs. No major complications occurred. In the follow-up for an average of 18.5 months,19 patients were free of adverse events except one with symptom recurrence. Conclusion As effective,simple and safe procedures,PTA and stenting can be used as a first-line treatment modality for the symptomatic patients with subclavian artery stenosis.

Objective To evaluate the prevalence and risk factors of human papillomaviruses (HPV)infection among human immunodeficiency virus(HIV)-positive women.Methods Totally 178HIV-positive and 122 HIV-negative women were enrolled.Structured interviews,peripheral CD4 + T cells counts and cervical specimens were obtained.Polymerase chain reaction(PCR)assay was used to identify HPV types. SPSS 16.0 was used for statistical analysis,and logistic regression was used to identify independent prognostic factors for high-risk HPV infection. Results HPV positive rate,high-risk and multiple HPV infection rates were 38.2% vs.12.3% ,35.4% vs.8.2% ,and 13.5% vs.3.3% in HIVpositive women and HIV-negative women,respectively,and the differences were of statistical significance (x2 =24.77,29.08 and 8.91,P ＜0.05).The common types of high-risk HPV were similar between HIV-positive and HIV-negative women(HPV16,52,58 and 18).CD4 + T count ＜ 350/pL,HIV-positive in husband,and HIV infection through sexual contact were risk factors for high-risk HPV infection in HIV-positive women.Conclusion sThe prevalence of HPV infection in HIV-infected women is high,especially for high-risk HPV infection and multiple infection.High-risk HPV infection usually occurs in HIV-positive women with low immune status,HIV infection through sexual contact and HIV-positive husband.

Objective To investigate the influencing factors for prehospital delay in patients with acute myocardial infarction (AMI).Methods A total of 807 consecutive patients with AMI who presented to the emergency department of Beijing Anzhen Hospital were analyzed. The influence of several potential risk factors on the prehospital delay time (PDT) was evaluated by comparing patients admitted more than 2 hours after onset of chese pain with those admitted within 2 hours after onset. Results Among 807 patients, 402 came to the hospital within 2 hours while the others arrived at the hospital after 2 hours. The median PDT was 130 min. Among the potential variables, advanced age, history of diabetes mellitus, occurrence of symptom at night and use of emergency medical service significantly affected PDT by multivariate analysis. Conclusion Interventions aimed at reducing the prehospital delay in AMI should primarily focus on the awareness of the risk and help-seeking behavior of patients.