Objective In order to analyse the problem that eligible water from factory will deteriorate during the transportation in the water supply networks. The aim of the present paper is to provide theoretic basis to ameliorate the water quality and make drinking water more secure. Methods Regarding water supply networks as a grey system, applying grey relational analysis to establish the attribution recognition model. The classification of water quality was treated as a grey sort and the state of water quality was a grey system. Water quality classified rank was determined and the water quality was analyzed according to the grey relation analysis theory. Results Three monitoring spots in north networks which included fourteen water quality gene was calculated. The relation degree matrix was educed. The result showed that the water quality of the first and the third monitoring spots belonged to grade one, and the second spot belonged to grade two. Conclusion Applying attribute recognition model based on the grey relation to analyze the water quality in the water supply networks, the case study shows that the grey relational analysis method is more reasonable, simple, reliable and informative. The effect of subjective factors is eliminated. The accuracy of model is verified by the experimental data in the end. There is a good agreement between the calculated result and actual condition.

[Objective]To study the dynamic changes of the VEGF and BMP-2 genes expressions inside the necrotic femoral head of rabbits.[Method]Made rabbit model of femoral head necrosis(FHN) with the method of twice intravenous injection with low dosage lipo polysaccharide(LPS)(10 ?g/kg body weight,at a time interval of 24 hours.)and 3 times intramuscular injection with methylprednisolone(MPS)(20mg/kg body weight,at a time interval of 24 hours.().)Immunohistochemistry,in situ hybridization and extract total RNA for real-time quantitative PCR were performed and successfully designed the suitable probes and primerswas used to detect the dynamic changes of the VEGF and BMP-2 genes expressions inside the femoral head of rabbits.[Result]The expressions of the VEGF and BMP-2 genes in group B(model group)at the 4,8,12 th and 16 th week were negative and real-time quantitative PCR showed that the level of the VEGF and BMP-2 mRNA genes expressions were obviously lower than that of control group.[Conclusion]The VEGF and BMP-2 genes expressions are inhibited in FNH model induced by LPS and MPS.

ObjectiveToobserve the killing effect of herpes simplex virus thymidine kinase/ganciclovir (HSV tk/GCV) suicide gene therapy system on human pancreatic carcinoma cell line. MethdsHSV tk gene was constructed into a retroviral vector pDOR neo. The recombinant plasmid pDOR tk neo was transfected into the retroviral packaging cell PA317. Finally HSV tk gene was transferred into human pancreatic cancer cell line PC 2. The sensitivity to GCV and bystander effect of PC 2/tk cells was tested in vitro. Antitumor effects were also observed after the administration of GCV in nude mice bearing tumor derived from PC 2/tk cells. ResultsHSV tk gene was successfully integrated into host cell. The killing effect of GCV on PC 2/tk cells was in a dose depandent and time depandent manners. The PC 2/tk cells showed a significant "bystander effect". Intraperitoneal injection of GCV postponed the formation of the implanted tumors. ConclusionPancreatic cancer cells expressing HSV tk can be effectively killed by GCV both in vitro and in vivo.

BACKGROUND:Although the preparation of bone tissue engineering scaffolds can achieve satisfactory results by solvent casting/particulate leaching, in situ molding method, electrospinning, phase seperation/freeze drying, gas foaming, there are stil some deficiencies in the accuracy, pore uniformity, spatial structure complexity, personalized stents. ＜br> OBJECTIVE:To prepareβ-tricalcium phosphate bone tissue engineering scaffolds using 3D printing. ＜br> METHODS:Drug-loadedβ-tricalcium phosphate scaffolds were prepared with 3D printing, and the structure was observed to measure its porosity and mechanical strength. The scaffold was immersed in simulated body fluid for 15 weeks to observe the quality change. The scaffold was co-cultured with rat bone marrow mesenchymal stem cells for 7 days to observe celladhesion and morphological changes. Rat bone marrow mesenchymal stem cells were cultured in extracts of drug-loadedβ-tricalcium phosphate scaffold and low-glucose Dulbecco's modified Eagle’s medium containing 15%fetal bovine serum for 24, 48, and 72 hours, to determine the absorbance values and cytotoxicity grading, respectively. Meanwhile, the cells were subjected to osteogenic culture for 1 week, and ＜br> the alkaline phosphatase activities in two groups were detected. ＜br> RESULTS AND CONCLUSION:The prepared scaffold showed irregular micropores, high porosity, uniform pore distribution, high pore connectivity rate, and large compressive strength. The drug-loadedβ-tricalcium phosphate scaffold degraded completely with 15 weeks, and cancellous bone defect repair was completed in the same period. Rat bone marrow mesenchymal stem cells adhered to the surface of drug-loadedβ-tricalcium phosphate scaffold and went deep into the scaffold, showing good growth and proliferation. The activity of alkaline phosphatase was also improved. These findings indicate that the drug-loadedβ-tricalcium phosphate scaffold has good biocompatibility.

Based on the survey of the learning preference of Kunming Medical College students,we analyze relationship between students’learning style and personalities.In order to match different learning style,teachers must find some strategies in their English teaching.

The simple and effective controlled needle devices were provided for acupuncture clinical trial so that the double blind acupuncture trial could be conducted possibly. The basic structure of filiform needle was modified. The needle handle was designed as hollow, the upper 1/3 of needle body as hollow and the lower 2/3 part as solid. The upper and lower parts were linked with an elastic unit. At the same time, the needle pad was attached. With this method, the new sham-acupuncture device was designed and developed. The new sham-acupuncture device could simulate needle insertion, manipulation and the techniques of reinforcing and reducing, without inserting into the human body. This innovation lays the foundation for perfecting the clinical randomized controlled blind trial of acupuncture.
Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Equipment Design ; Needles

Objective To study genomic polymorphic DNA and genetic distance of 7 species of ticks.\ Methods\ Ticks used in this study were Dermacentor nuttalli, D.silvarum, Haemaphysalis qinghaiensis, H.formosensis, H.punctata, Amblyomma testudinarium, and Ixodes ovatus. DNA extracts of the 7 species of ticks were amplified by random amplified polymorphic DNA (RAPD) and PCR technique using 5 primers with different arbitrary single chain polynucleotide sequences. DNA fingerprint maps were analyzed and the genetic distance among 7 species of ticks were counted. \ Results \ The amplified products of the 7 species of ticks by RAPD all showed their specific DNA band. The average genetic distance among them was 0\^71. Conclusion RAPD can differentiate the 7 species of ticks.

Objective:To evaluate the effects of magnetic attachment keepers on MRI images.Me-thods:In in vitro part,keepers of MAGFIT EX 400,MAGFIT EX 600 and MAGNEDISC 800 magnetic attachment systems were cast into standard root-caps with different alloy respectively,including nickel-chromium alloy and gold-palladium alloy,or not be cast for contrast.In in vivo part,volunteers with keepers in different position and amount were included.All the specimens and volunteers were imaged by a Siemens SONATA 1.5-T MRI scanner.Extent of the artifacts in every slice was measured.The magnitude of the artifacts and the distortion of the anatomic structures were compared.Results:All kinds of keepers being studied produced obvious MRI artifacts.The extent of MRI artifacts induced by MAGFIT EX 400 keepers,MAGFIT EX 600 keepers and MAGNEDISC 800 keepers are 158.94 mm,168.52 mm and 173.00 mm,respectively.The images of mental region,tongue,palate,jawbone and sinus were evidently obscured by artifacts in all cases.When keeper was put in the molar region,the keeper-related MRI artifact may obscure the imaging of brain,spinal cord.SE sequence with right-left frequency-encoding direction was more desirable in MRI for the patients with magnetic attachment keepers.Conclusion:Keepers of magnetic attachment system did induce obvious artifacts in MRI examination.

The scientific research management is one of the critical factors to build up the innovative system of scientific research.There are two modes of scientific research management:self-organization and hetero-organization.The serf-organization management,which follows the rules of science development,is the precondition to advance the scientific research,it is also necessary to implement the hetero-organization in right time and right place.The management should conduct more investigation into the nature of scientific research,put the service on the important role and finally speed up the reforming of science research management mechanism.

BACKGROUND: Following peripheral nerve transplantation, the time phases and law of growth associated protein 43 (GAP-43) expression in sensory neuron soma of the corresponding dorsal root ganglia remain unclear.OBJECTIVE: To investigate the expression of GAP-43 at the corresponding dorsal root ganglia following sciatic nerve transplantation in rats.METHODS: Adult male Wistar rats were randomly divided into 2 groups: control group (sham-operated group) and experimental group (nerve transplantation group). The rats were killed at 3 days, 1, 2, 4, 6, and 8 weeks after operation and the dorsal root ganglia U-s of every rat was taken for observation. The expression of GAP-43 mRNA and protein in the corresponding segments of spinal cord were detected by RT-PCR and Western-blot techniques.RESULTS AND CONCLUSION: GAP-43 mRNA showed a low level of expression in the control group, which had no obvious changes in different phases. In the experimental group, GAP-43 mRNA expression was observed remarkably in the dorsal root ganglia at the first week, reached a peak at the second week and gradually descended from the sixth week. The GAP-43 protein expression showed the same laws as that of GAP-43 mRNA. The results demonstrated that there exists damage reactivity change in the regeneration ability of neurons.