Objective:To explore the effect of phytic acid on apoptosis of SGC-7901 cells and its mechanisms. Method:The inhibiting action of phytic acid on SGC-7901 cells was examed by MTT assay,and the effect on apoptosis of SGC-7901 cells was examined by DNA gel electrophoresis,and the expressions of Fas,Bax and caspase-3 protein were detected by Western blot method. Results:From MTT assay,we observed that phytic acid inhibited the growth of SGC-7901 cells in dose and time dependent manner. Typical DNA ladder was found during gel eletrophoresis. Caspase-3 can be activated by phytic acid and the expressions of Bax and Fas protein in phytic acid group were higher compared with control group,and increased in a dose-dependent manner. Conclusion:Phytic acid can induce apoptosis of SGC-7901 cells which may be correlated to apoptosis associated protein Fas,Bax and caspase-3.

Objective:To establish an HPLC method for the determination of isomeric impurity in fasudil hydrochloride. Meth-ods:The chromatographic method was carried out on a Kromasil 100-5 Phenyl C18 column with phenyl bonded silica as the filler (250 mm × 4. 6 mm, 5 μm), and phosphate buffer (10 mmol· L-1 ammonium dihydrogen phosphate, adjusting pH to 4. 0 with 1% phos-phoric acid) -acetonitrile (80∶ 20) was used as the mobile phase. The detection wavelength was 275nm and the column temperature was 40℃. The flow rate was 1. 0 ml· min-1 , and the injection volume was 10 μl. Results:Fasudil hydrochloride and its derivative was linear within the range of 0. 148-2. 960 μg·ml-1(r=1. 0000) and 0. 101-2. 014μg·ml-1(r=0. 999 9), respectively. The av-erage recovery of isomer impurity in fasudil hydrochloride was 101. 9% with RSD of 0. 98%(n=9). Conclusion:The method is sim-ple,accurate and reproducible,which can be used for the quality control of fasudil hydrochloride and its isomer.

Objective To investigate the influencing factors related to the complications of esophageal foreign body in children. Methods Data of 150 children with esophageal foreign bodies admitted to Xi'an Children' s Hospital from January 2012 to June 2015 were included in the retrospective analysis. Related clinical variables ( gender, age, location, time, size, sharpness, quality, and severity of complications) were statistically analyzed. Results Spearman correlation analysis showed that the age of children was negatively correlated with esophageal foreign body complications (r=-0. 187, P=0. 022), incarceration time ( r=0. 456, P<0. 001) , sharpness ( r=0. 384, P<0. 001) and quality ( r=0. 234, P=0. 004) was positively correlated with the incidence of complications. Non?conditional Logistic regression analysis for polytomous ordinal response showed that incarceration longer than 8 h yielded complication risks 9. 507 times as much as that less than 8 h ( 95%CI:2. 982?30. 309) . Obtuse or sharp foreign body yielded risk 142. 751 times as smooth foreign body did (95%CI:13. 736?1483. 562). Conclusion Incidence and severity of complications of esophageal foreign body in children are closely related to the age of the children, incarceration time and the degree of sharpness. For patients of less than 1 year old, incarceration for more than 8 hours, with an obtuse or sharp foreign body, early diagnosis and treatment is essential.

Objective To explore the feasibility, safety and the efficacy of peroral endoscopic myotomy (POEM) for pediatric patients with achalasia.Methods A total of 21 patients (mean age 2 years, range 11 months-7 years) with AC were enrolled and underwent POEM from January 2012 to December 2014.Procedure-related complications, reflux esophagitis were observed.Eckardt score and the pressure of the lower esophageal sphincter (LES) were analysed.Results All patients underwent POEM successfully.No serious POEM-related complications were observed.During a mean follow-up period of 20.4 months (range 9-36 months), mean Eckardt score decreased from 8.1 to 0.8 after treatment (P ＜ 0.01).Mean LES treatment also decreased from 30.2 mmHg to 11.5 mmHg after the operation (1 mmHg =0.133 kPa, P ＜ 0.01).Reflux esophagitis developed in four patients (19.0％ ,4/21).Conclusion POEM is safe and effective treatment for pediatric patients with achalasia.

Objective To compare the effica cy of three N,N-diethyl-m-toluamide (DEET) formulations against Schistosoma japonicum infection in mice. Methods Three 10% DEET formulations (DEET isopropanol solution,DEET cr eam and DEET vaseline ointment)were prepared and two experiments, no-dipping or dipping after applying DEET preparation ,were designed. In each experiment, mice were randomly divided into three experiment groups and three control groups . D ifferent DEET preparations were applied in the experiment groups and isopropanol solution, “yumeijing” cream , vaseline ointment were applied in the control g roups, respectively. At different time points after skin application (0.5, 1, 2 , 4 h and 8 h),the mice were infected with (50?5) Schistosoma j apon icum cercariae on the abdominal skin in no dipping experiment. I n dipping experiment,after mice skin were applied and were dipped for 10,30 min, 1 ,2 h and 4 h, respectively, the mice were infected with (50?5) cercariae. Af ter 6-7 weeks ,all the mice were sacrificed and the worms were collected by hea rt perfusion and counted.Worm reduction rates were calculated and rank sum tests w ere carried out. Results In no-dipping experim ent,three DEET formulations provided 100.0% protection in mice at least for 1 h .A t 2 ,4 h and 8 h ,the worm reduction rates were 94.8%,89.9% and 13.3% for DE ET isopropanol solution, 100.0%,97.8% and 50.7% for DEET cream and 100.0%,9 9.0% and 9 8.0% for DEET vaseline oinment, respectively. In dipping experi-ment, given 50.0% wor m reduction rate as efficient in preventing penetration by the cercariae, the e ffective times were 10 min for DEET isopropanol solution,30 min for DEET cream a nd 2 h for DEET vaseline oinment,respectively. Conclusion Three DEET formulations are effectiv e in preventing Schistosoma japonicum infection in mice.DEET vaseline oinment can provide better protection than the other two D EET formulations.

ObjectiveTo explore the efficiency of N,N-diethyl-m-toluamide (DEET) with different concentrations in prevention of Schistosoma japonicum infection in mice.MethodsKunming-mice were randomly divided into four groups for the application of the vaseline ointment with different DEET concentrations (0,10%,20% and 30%). After the abdominal skin of the mice were smeared with those DEETs above mentioned and dipped into water for 10,30 min,1,2 h and 4 h, the mice were infected with (505) cercariae respectively. After infected for 6-7 weeks, all of the mice were sacrificed,and by perfusing heart the worms were collected and counted.Worm reduction rates were calculated and the rank sum tests were carried out. ResultsAt the time points of 1,2 and 4 h , the worm reduction rates were 78.34%,63.15% and 40.23% for 10% DEET vaseline ointment, 98.61%,93.37% and 75.74% for 20% DEET vaseline ointment and 100.00%,98.61% and 93.07% for 30% DEET vaseline oinment respectively(H =10.06,10.84,10.80,all P

Objective:To explore the relationship between drug evidence and core prescription for depression.Methods:We retrieved literature of TCM for depression from CNKI, VIP and Wangfang databases to November 2019, 30th as well as there cords from Ancient and Modern Medical Records Cloud Platform (V 1.5). The Excel 2010 was used to establish the standardized database of medical records. After the standardization of medicines, Ancient and Modern Medical Records Cloud Platform (V1.5) statistics methodswere used for association rules analysis, complex networks, and analysis of drugs’ frequency, medical characteristics, core prescription drugs.Results:A total of 632 effective prescriptions were included, involving a total of 527 drugs. The results of frequency of herbs showed that 23 kinds of high-frequency herbs were obtained. Bupleuri Radix was the most frequently used medicine. Most herbs are warm or flat, with pungent, sweet and bitter in taste, belonging to the lung, liver, heart and spleen meridians. A total of 25 drug-pair association and 13 TCM association were obtained by association rule analysis. Conclusions:TCM treatment for depression is mainly based on soothing the liver and regulating qi, clearing the heart and calming the nerves. Bupleuri Radix, Curcumae Radix, Paeoniae Radix Alba, Chuanxiong Rhizoma, Ziziphi Spinosae Semen are the basic prescriptions. Angelicae Sinensis Radix, Ophiopogonis Radix, Albiziae Cortex, Polygalae Radix, Poria are used as reference.

Objective To compare the different doses of low-temperature plasma (LTP) on wound healing in BALB/c mice so as to discuss the effects of the optimal dose of low-temperature plasma dealing with wound in mice and the acting mechanism of wound healing.Methods Adoptatmospheric pressure plasma jet discharged by the dielectric barrier was used to treat mouse skin wound.According to the processing time, the wounds were divided into 10s, 20s, 30s, 40s and 50s experimental groups, while naturally healing wounds served as negative controls and the wounds dealt with recombinant human epidermal growth factor served as positive controls.We recorded the wound size every day, observed the histopathological changes, the expression level of type Ⅰ collagen by immunofluorescence, and analyzed the composition of low-temperature plasma jet.Results The wounds with plasma treatment time of 10s, 20s, 30s, and 40s showed significant daily improvement and almost complete closure at days 12, 10, 7, 13, respectively.However, the wounds with plasma treatment time of 50s remained unhealed atday 14.The wounds in positive control group all healed, and the wound healing effect in positive control group could be achieved in 30s group.HE staining and immunofluorescence staining assays showed the optimal result of epidermal cell regeneration, granulation tissue hyperplasia, and collagen deposition in histological aspect at day 7 in 30 s group.The low-temperature plasma jet contained highly reactive free radicals of nitrogen and oxygen, which play an important role in wound healing process.Conclusion Appropriate doses of cold plasma can accelerate wound healing whereas over-doses of plasma can suppress wound healing.The process of wound healing may be related to reactive oxygen and nitrogen species in LTP.

In this study ,we aim to identify the protein interaction site of microneme protein 2 (MIC2) and aldolase in Toxoplasma gondii .The tryptophan (Trp ,W) at site 767 of carboxyl terminus of MIC2 (MIC2C) was mutated into alanine (Ala ,A) by site-directed mutagenesis to construct plasmid MIC2C W/A/pGEX-4T-1 .The mutant protein GST-MIC2C W/A was expressed in E .coli upon IPTG induction .Glutathione sepharose beads were incubated with GST-MIC2C W/A and GST-MIC2C respectively ,then incubated with tachyzoite lysates ,and bound proteins were eluted using sample buffer .Eluants were resolved by SDS-PAGE and Western blot .A protein band specifically recognized by anti-aldolase antibody was detected in prod-ucts coming from GST pull-down of GST-MIC2C ,but not in pull-down products coming from GST-MIC2C W/A .With muta-tion of MIC2C W767 to A ,MIC2 protein lost the binding ability to aldolase .Tryptophan (W767 ) was the protein interaction site of MIC2 and aldolase in T .gondii .

Objective To explore susceptibility of praziquantel(PQT) against Schistosoma japonicum in the repeated chemotherapy areas in Dongting Lake region of China. Methods Sixty mice were divided into two groups, and infected respectively by cercariae released from the infected snails which were collected from new and old endemic areas. After 5 weeks, the mice in each group were divided into control groups and treatment groups (PQT group). The mice in each PQT group were treated with a single oral dose of praziquantel (600 mg/kg). Three weeks post treatment, mice were dissected, and the number of adults, the stool eggs per gram (EPG), the liver EPG and the hatching rates were observed. Results The worm reduction rates of the PQT groups of new and old epidemic areas were 98.24% and 98.71% respectively, and the stool egg reduction rates 99.94% and 99.64%, the liver egg reduction rates 75.85% and 73.10%,and there were no significant differences between the new and old endemic areas. The stool hatching test was positive in the control groups, and negative in the PQT groups. Conclusion Susceptibility of praziquantel against Schistosoma japonicum does not decrease in repeated chemotherapy areas in Dongting Lake region.