ve To improve the method of determination of aluminium in water. Methods The method of aluminium chrome azurol S-cetylpyridinium bromide spectrophotometry was used. Results The improved determi-nation of aluminium revealed perfect linear relationship in determining the standard aluminium solution series at the range of 0. 0-6.0?g/25 ml aluminium. The regression equation was calculated as y = 1. 031x + 0. 008(r=0.9994) . The detection limit was 0.02 mg/L aluminium. The coefficient variation was below 4.90% . The recovery rate ranged from 93.3% to 100.0% . Conclusion The improved method was rapid and easy to operate, suitable for determination of Al3+ in a large number of water samples.

We adjusted medical statistics teaching contents and teaching methods according to the characteristics of different students and based on the current status of domestic medical statics teaching.Implementation effect was summarized in order to provide references for the teaching reform of medical statistics.

The immune system of bacteria against phage shares a lot of similarity with that of mammals, especially the adaptive immune system.The elements and components of the bacterial adaptive immune system———clustered regularly interspaced short palindromic repeats ( CRISPR ) and the mammalian adaptive immune system have a lot of parallel mechanisms.We could acquire new understanding about the immune function of CRISPR systems through that analogy.In recent years, researchers have found CRISPR-Cas system can play significant roles in regulating bacterial growth and metabolism.These researches have revealed new functions of CRISPR beyond immunity.The ability of CRISPR to affect gene expression has attracted increasing attention.Further studies are needed to shed light on the complicated functions of CRISPR.

BACKGROUND: The risks in pregnancy to the developing fetus result in cerebral palsy after delivery. Therefore, the study on etiology of cerebral palsy should transfer to biological field of embryonic development and stress on multi-factor analysis on relevant environment of pregnant mother,hereditary factors and relevant diseases.OBJECTIVE: To analyze the main risks of incidence of infantile cerebral palsy.DESIGN: Pair design at ratio of 1:2 and Logistic regression analysis of multi-factors were applied.SETTING: Prevention Medical Research Room of Jiamusi University and Department of Epidemiology, College of Public Health, Harbin Medical University.PARTICIPANTS: Totally 103 cases of infantile cerebral palsy were collected from Jiamusi Treatment and Education Center of Cerebral palsy,Shanyang Pediatrics Hospital and Qingdao Pediatrics Hospital from December 2002 to September 2003, of which, 68 cases were male and 35 cases were female. The guardians were in the know and agreed with the investigation. The pair principle at ratio of 1:2 was adopted, based on which, 206 cases of sick children with non-cerebral palsy and disorders in neurological system and healthy children treated at same period in the hospitals were collected as the control. The match conditions in the control were same sex, same nationality and difference in age ± 3 months.sy and healthy children was self-designed. The investigated items included mainly: Factors before delivery (hereditary factors): family history of similar disease; mother's factor: general situations, such as occupation, educational background and age of delivery; behavioral factors of birth: including times of pregnancy, times of delivery and abnormal delivery; healthcare and nutrient in pregnancy: such as examination before delivery and intakes of various foods; sickness or abnormal manifestations in pregnancy: including pathogen infection, pregnant syndromes and vaginal bleeding; medication in pregnancy, fetal placents factors: such as neck winding, mass weight of birth, twin pregnancy, factors during delivery (complications in delivery,methods of delivery) and neonatal factors (various kinds of disorders in neonates). Questionnaire of investigation was performed by looking-up medanalysis was done on single factor and multi-factors. The factors that presented statistical significance in single-factor analysis, significant difference (P ＜ 0.05) and P value near to 0.05 were adopted to establish the model of multiple Logistic regression of main risks of cerebral palsy and conditional Logistic regression analysis was carried on.MAIN OUTCOME MEASURES: Analysis on the effects of factors before delivery, during delivery and in neonatal phase on incidence of cerebral palsy.RESULTS: Totally 103 cases of cerebral palsy and 206 cases of healthy factors: Occupation of mother was associated with cerebral palsy. Worker and age ≥34 years were the risks of cerebral palsy (OR=13.333, 2.864,eating fish and sea products in pregnancy, pre-delivery body temperature,mass weight of birth ＞ 4 000 g and pregnant weeks of 37 to 42 were the protective factors of cerebral palsy (OR=0.028-0.576, P ＜ 0.05-0.01); abnormal delivery history and vaginal bleeding in pregnancy were the risks of ery: Apgar score and caesarian section were the protective factors of cerebral palsy (OR=0.458, 0.343, P ＜ 0.01); forceps delivery and delivery with oxytocin were the risks ofcerebral palsy (OR=16.338, 2.116, P ＜ 0.01,of newborn and neonatal intracranial hemorrhage were the risks of cerebral sion analysis: Forceps delivery was the highest risk (OR =70.668) and the sequence risk degree from high to low was asphyxia of newborn, elder age of pregnant mother, vaginal bleeding in pregnancy and abnormal mass weight of birth (OR=19.083-4.138, P ＜ 0.05-0.01). Eating fish and sea products in pregnancy and caesarian section were the protective factors (OR=0.324; 0.262, P ＜ 0.01, 0.05).CONCLUSION: Risks of cerebral palsy are mainly focused on pregnant period and perinatal period. It is suggested to prevent and eliminate actively various risks of cerebral palsy before, during and after delivery (such as forceps delivery, asphyxia of newborn, elder age of pregnant mother,vaginal bleeding in pregnancy and abnormal mass weight of birth) so as to reduce the incidence rate of cerebral palsy.

Studies have shown that co-infection of influenza viruses with bacteria is an important cause of high mortality during the epidemic of influenza.There are at least 12 species of bacteria that have been reported to be able to co-infect with influenza.Among those species,co-infection with Staphylococcus aureus is not only the most common but also the most lethal.However,the pathogenesis of high mortality from co-infection with influenza virus/S.aureus remains elusive.In addition,co-infection of influenza virus/S.aureus can induce severe pneumonia.There is new evidence that influenza virus can reduce the host′s tolerance to pathogenic or inflammatory injury,and the two pathogens can also synergistically aggravate toxic effects on the host.Here,we review the mechanisms of severe mortality of influenza infection associated with S.aureus co-infections in order to contribute to prevention and control of influenza in the future.

Blue laser imaging (BLI) is a new endoscopic system equipped with the laser beam emitting two different wavelengths.It produces bright and high resolution images for observation of microvascular and microsurface patterns of esophageal and gastric mucosa, helping the diagnosis of early upper gastrointestinal cancer.Compared with the existed endoscopic techniques, BLI shows its unique advantages.In this article, advances in application of BLI in diagnosis of early upper gastrointestinal cancer were reviewed.

Objective To review and summarize the experiences of mitral valve replacement in the children aged less than five years.Methods Twelve patients with moderate to severe mitral valve regurgitation (MR) or/and severe mitral valve stenosis (MS) were retrospectively study from January 2008 to December 2011,all of them suffered from severe heart failure and underwent mechanical mitral valve replacement.There were 9 males and 3 females.They aged from 4 to 58 months [mean (26.2 ± 18.1) months] and weighted from 5.6 to 13.0 kg [mean (9.6 ± 3.8) kg].Three patients underwent unsuccessful mitral valve repair before mitral valve replacement.17 to 23 mm aortic bileaflet mechanical prosthesis were reverse directional implanted in nine patients and 25 to 27 mm mitral bileaflet mechanical prosthesis were implanted in three patients.Results One patient died,the operative mortality rate was 8.3％.2 patients were complicated by cardiac arrhythmia and 2 patients complicated by mild hemolysis,all recovered after symptomatic treatment.The heart function of servived 11 patients improved significantly,none of them were complicated by haemorrhage or thromboembolic.Conclusion Severe mitral valve lesion injure heart function significantly for the young children and timely surgical intervention is the only option.Mitral valve replacement is the ultimate option for the patients failed in mitral valve annuloplasty.The introduction of aortic bileaflet mechanical prosthesis which smaller size is available and reverse directional implantation resolve the difficulty of prosthesis-patient mismatch essentially,most children with a small prosthesis has to redo mitral valve replacement with a larger size prosthesis.The compliance of permanent anticoagulation of young children is favourable and more intensively follow-up is essential to avoid the incidence of bleeding and thromboembolic.

Objective To explore the feasibility of establishing a swine model of liver cirrhosis with portal hypertension by portal infusion of 80％ alcohol.Methods A total of 13 Guizhou miniature pigs were randomly divided into three groups,experiment group 1 (n=5),experiment group 2 (n=5) and control group (n=3).Experiment groups of pigs received portal infusion of 80％ alcohol in volumes of 5 ml in group 1,and 10 ml in group 2,and the pigs in control group received portal perfusion of saline in volumes of 10 ml.All animals were performed direct portal angiography,the portal vein pressures and diameter were also detected before,immediately and 6 weeks after the infusion.All animals underwent liver biopsies before and 6 hours,1-6 weeks after operation.And contrast-enhanced abdominal CT was performed before and 6 weeks after operation.All animals were dissected 6 weeks after operation,aud each leaf of liver specimens were performed histological examination.Results There was no statistically significant difference of the portal venous pressure and diameter before infusion and 6 weeks after infusion in the experiment group 1 and control group (all P＞0.05).In the experiment group 2,compared with pre infusion,the portal vein pressure and diameter were higher than those of immediately and 6 weeks after infusion (all P＜0.05).In both experiment group 1 and group 2,all pigs had developed into liver fibrosis,the METAVIR score of 2 pigs in group 1 and 5 pigs in group 2 respectively were up to grade 4.Conclusion Portal infusion of 80％ alcohol is more suitable for establishing a swine model of liver cirrhosis with portal hypertension.

Objective To investigate the effect of arthroscopic debridement and meniscectomy in treatment of moderate or severe meniscus injury combined with knee osteoarthritis in early or middle stage. Methods 156 cases diagnosed with moderate or severe meniscus injury combined with knee osteoarthritis in early or middle stage were collected from October 2011 to October 2014. Lysholm knee score and preoperative examinations such as anteroposterior, lateral, axial radiographs, the standing full leg length X-ray film and MRI scan of the knee were recommended to definitively understand the osteoarthritis staging and meniscus injury grading. All patients were treated with arthroscopic debridement and meniscectomy. After operation, physical rehabilitation exercises and regular clinical follow-up were carried out as planned. The Lysholm knee score data from preoperation and terminal follow-up was statistical analyzed. Results No patient experienced any perioperative and postoperative complications. Statistical analysis showed that the Lysholm knee score of postoperation was significantly higher than that of preoperation [(87.3 ± 7.9) vs (67.5 ± 4.9), P < 0.05). Conclusion Arthroscopic debridement and meniscectomy in treatment of moderate or severe meniscus injury combined with knee osteoarthritis in early or middle stage, gains beneficial effects for its minimal invasion and quick recovery.

Objective:To construct a human Fab phage antibody library and to obtain some recombinant clones which can express Fab fragment antibody against ? 1-adrenergic receptor.Methods:Fd heavy chain gene and ??? light chains gene of IgG obtained by RT-PCR from peripheral lymphocytes of DCM patients whose anti ? 1-AR antibodies are present were cloned into pComb3 vector and the human Fab phage antibody library was constructed.The library was panned by phage display technology with ? 1-ARECⅡ as antigen.Results:A human Fab phage antibody library with 1.4?10 6 capability was constructed successfully,a positive clone against human ? 1-AR was screened from the phage antibody library.Conclusion:The recombinant clones which express Fab antibody against ? 1-AR can be obtained by phage display technology.