Objective To investigate the regulatory T cells (Treg) induced by 2, 3, 7, 8-tetrachloro-dibenzo-p-dioxin (TCDD) on the aryl hydrocarbon receptor activator from na?ve T cells, in collagen-induced arthritis (CIA)-rheumatoid arthritis (RA) mouse in the correction of rheumatoid arthritis pathological process on Th17/Treg cell imbalance. Methods ① Rat CIA model was established by bovine Ⅱ collagen injection. The rats were divided into 3 groups: the normal group (N), the CIA model group (C), and the TCDD group (T).② The percentage of Th17 and Treg from peripheral blood mononuclear cells (PBMCs) were analyzed with flow cytometry (FCM). Th17 and Treg cells related cytokine including interleukin (IL)-1β, IL-6, IL-17A, IL-23, tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β were measured with enzyme linked immunosorbent assay (ELISA). ③ The changes of the arthritis parameters, and radiological of T/NT before and after treatment were observed. ④ After two weeks of treatment, the rats were killed. The swollen joints were used for pathological assessment and arthritic pathology injury score was evaluated. ⑤ All data were analyzed by one-way analysis of variance (ANOVA) test and t test. Results In group C:When the second immunization was compared with the first, the percentage of Th17 cells was significantly increased [(2.99 ±0.16)%, (4.02 ± 0.33)%, t=-6.82, P<0.01], while Treg cells were significantly reduced [(2.67 ±0.57)%, (1.79 ±0.39)%, t=3.11, P=0.000], Th17/Treg was significantly increased (1.14 ±0.14, 2.27 ±0.39, t=-4.53, P=0.039). In group T, the percentage of Th17 cells were significantly reduced [(2.69±0.24)%, (1.21±0.25)%, t=5.12, P<0.01] before and after treatment, while Treg cells were significantly increased [(2.76 ±0.36)%, (3.78 ±0.22)%, t=-6.24, P<0.01], Th17/Treg was significantly reduced (1.08±0.07, 0.21±0.05, t=-11.92, P=0.027). In group C:When the second immunization was compared with the first, the Th17 cells related cytokines were significantly increased inclu-ding IL-1 [(96±12) pg/ml, (153±18) pg/ml, t=-13.24, P<0.01], IL-6 [(246±14) pg/ml, (295±15) pg/ml, t=-9.41, P=0.000], IL-17 [(76 ±14) pg/ml, (99 ±16) pg/ml, t=-5.78, P<0.01], IL-23 [(85 ±9) pg/ml, (102 ±11 ( pg/ml, t=-11.71, P<0.01], TNF-α [(303 ±12) pg/ml, (345 ±17) pg/ml, t=-15.56, P=0.007], while Treg cells related cytokines TGF-β was significantly reduced [(42 ±7) ng/ml, (35 ±8) ng/ml, t=7.52, P=0.012]. Th17 cells related cytokines were significantly reduced including IL-1 [(93 ±10) pg/ml, (79 ±8) pg/ml, t=10.52, P<0.01], IL-6 [(245 ±11) pg/ml, (151 ±8) pg/ml, t=19.23, P<0.01], IL-17 [(76 ±10) pg/ml, (62 ±9) pg/ml, t=7.37, P=0.005], IL-23 [(84 ±11) pg/ml, (38 ±6) pg/ml, t=14.48, P=0.000], TNF-α [(294 ±8) ng/ml, (195 ±9) ng/ml, t=23.35, P<0.01], while Treg cells related cytokine TGF-β was significantly increased (41 ±8, 61 ±10, t=-10.61, P=0.000. Conclusion TCDD can increase Treg cells, reduce Th17 cells, so the Th17/Treg cell in RA patho-genesis could be re-balanced.

OBJECTIVE:To evaluate the clinical efficacy,safety and status of gemcitabine for non-small-cell lung cancer (NSCLC) systematically.METHODS:Included studies retrieved from Medline,EMbase,Cancerdit,CBM and CNKI were evaluated by two reviewers alone and cross-checked.The Meta-analyses of studies were conducted using RevMan 4.2.2 software.RESULTS:12 studies containing 1,715 patients with NSCLC were included.12 studies were all reported randomly(RCT).Hidden information and application of blind method in therapy were not reported.Results of meta-analysis indicated that:(1)After chemotherapy reaction rate and the level of median disease development in GP therapy (gemcitabine combined with cisplatin) were higher than in GC therapy (gemcitabine combined with carboplatin) with similar median survival time and strong reaction of digestive tract;(2)Reaction rate;the level of median disease development and median survival time in GP therapy than in EP therapy (etoposide combined with cisplatin) but with strong toxicity in bone marrow;(3)Reaction rate of GP therapy was same to NP therapy (vinorelbine combined with cisplatin) with strong toxicity in bone marrow;(4)Reaction rate,median survival time and one-year survival rate in GP therapy were similar to in gemcitabine alone therapy with intense reaction in digestive tract;(5)Reaction rate in VGP therapy (vinorelbine,gemcitabine combined with cisplatin) was higher than in VG therapy (vinorelbine combined with gemcitabine) with strong toxicity in bone marrow but they had same the level of median disease development and median survival time;(6)Reaction rate,median survival time in DG therapy (gemcitabine combined with taxotere) were higher than in taxotere alone therapy with advanced the quality of life and strong toxicity in bone marrow;(7)Reaction rate,median survival time,the level of median disease development and one-year survival rate in therapy of gemcitabine (50 mg?min-1?30 min,i.v) were similar to that of gemcitabine (10 mg?min-1?150 min,i.v) with intense toxicity in bone marrow.17 cases of related death were reported in all researches.CONCLUSION:Gemcitabine has exact clinical efficacy and causes strong toxicity in bone marrow and reaction in digestive tract.

0.05 for both efficacy(?2=16.229 2)and safety(?2=16.672 0).In combined test for efficacy,the combined OR=2.685 1(95% CI:2.388 3～3.018 7) and the test for combined OR showed that ?2=43.835 8(P

OBJECTIVE:To study the effect of kallidinogenase on senile diabetic nephropathy.METHODS:95senile cases with diabetic nephropathy treated with the basic therapy were randomly divided into treatment group(plus kallidino?genase)and control group(plus aspirin),the proteinuria ejection level,hemorheological parameters and blood lipid level of2groups in24hours were investigated before and after treatment.RESULTS:As compared with prior treatment and the coutrol group,the proteinuria ejection level decreased significantly,the hemorheology improved and the blood lipid level decreased in the treatment group.CONCLUSION:Kallidinogenase is effective to some degree in the prevention and cure of senile diabetic nephropathy and diabetic macroangiopathy.

The research of urologic reconstruction with tissue engineering techniques has been carried out for a couple of years.However,the clinical application is impeded by the difficulties in drawing materials and lack of sufficient cells for seeding.The stem cells have the ability of self-renewal and differentiating into desired cell lines,which also have the advantage of less invasive to be harvested,provide new opportunities for further research.Mesenchymal stem cells(MSCs) have been used in the autologous augmentation cystoplasty in recent years,and bone marrow derived mesenchymal stem cells can undergo directed differentiation toward to endodermal derived urothelium in the appropriate signaling environment.The stem cells were supposed to differentiate in urethral tissue like cells in bladder tissue according to the similar inner environment.Transurethral ultrasound(TUUS)-guided injections of autologous myoblasts and fibroblasts is an effective and minimally invasive treatment modality to treat stress urinary incontinence.In addition,eNOS-transduced syngeneic MSCs can improve erectile function through accelerating eNOS expression and improving endothelial-derived NO biosynthesis.

Objective:To evaluate the cost-effectiveness of levofloxacin and gatifoxacin in the treatment of lower respiratory tract infections.Method:The clinical data of levofloxacin and gatifoxacin in the treatment of lower respiratory tract infections were collected with the evidence-based medicine method and were evaluated with pharmacoeconomic cost-effectiveness analysis.Result:The cure rates of two drugs were 64.29%(57.70%,70.88%),and 81.43%(70.05%, 92.81%)and the cost-effectiveness ratios were 1.90 and 5.79 respectively.The?C/?E was 20.37.Conclusion:The cost-effectiveness analysis indicated that the cost-effectivenss ratio of levofloxacin is superior to that of gatifoxacin though the cure efficacy of gatifoxacin for lower respiratory tract infections is better.

Evidence-based anesthesia’s core thinking is to abide by evidence,which means clinical decision must be based on objective research result,personal experiences and patients` actual situation. The thinking of EBA will magnificently promote the conversion of model of clinical anesthediology education,and will make significant influence on clinical teaching,scientific research, and clinical practice. According to the current situation,try to give out new teaching model of evidence-based practice of anesthesiology and offer teaching methods and practical procedures.

Brain cell microenvironment,also known as cerebral tissue channel,consists of the brain extracellular space and its contents.For a long time,because of the limit of technology,the research about brain extracellular space hasn't been given enough attention in the field of cognitive sciences and neuroscience.With the development of medical imaging technology,the research about brain extracellular space will open up a new space for brain and cognitive sciences,and provide a new approach for diagnosis and treatment of encephalopathy.Based on the method of medical informatics,this article reveals hot topics on brain science,presents the history of the research about brain cell microenvironment,and analyzes the construction of neurology literature so as to provide reference for the future researchers.

Objective　To research the procedure of male face-lift. Methods　According to the distribution features of skin and hair in the male face, the flap assigment at the incision point was improved. Results　From 1995 to 2000,25 cases of male facelift were performed, aged from 37 to 68. Postoperative follow-up of 1 to 24 months showed the results were satisfactory. Conclusion　 Male face-lift has satisfactory rejuvenation results by improving the incision design and skin-lifting technique.

[Objective]To study the dynamic changes of the VEGF and BMP-2 genes expressions inside the necrotic femoral head of rabbits.[Method]Made rabbit model of femoral head necrosis(FHN) with the method of twice intravenous injection with low dosage lipo polysaccharide(LPS)(10 ?g/kg body weight,at a time interval of 24 hours.)and 3 times intramuscular injection with methylprednisolone(MPS)(20mg/kg body weight,at a time interval of 24 hours.().)Immunohistochemistry,in situ hybridization and extract total RNA for real-time quantitative PCR were performed and successfully designed the suitable probes and primerswas used to detect the dynamic changes of the VEGF and BMP-2 genes expressions inside the femoral head of rabbits.[Result]The expressions of the VEGF and BMP-2 genes in group B(model group)at the 4,8,12 th and 16 th week were negative and real-time quantitative PCR showed that the level of the VEGF and BMP-2 mRNA genes expressions were obviously lower than that of control group.[Conclusion]The VEGF and BMP-2 genes expressions are inhibited in FNH model induced by LPS and MPS.