Objective To establish the quality standard for Jianpi Fugan Pills. Methods Angelicae Sinenis Radix and Atractylodis Macrocephalae Rhizome in the formulation were qualitatively identified by TLC. The content of ferulic acid was analyzed by HPLC. TIANHE C18 column (250 mm×4.6 mm, 5 μm) was used with a mobile phase of acetonitrile-0.085% phosphorous acid (17∶83). The content was detected at wavelength of 323 nm. The flow rate was 1.0 mL/min, and temperature was 25 ℃. Results The spots in the TLC of Angelicae Sinenis Radix and Atractylodis Macrocephalae Rhizome were in the same chormatograms of control articles. The linear range of ferulic acid was 0.114-1.482 μg. The average recovery was 98.36%and RSD was 1.16%. Conclusion The method is simple, accurate and highly sensitive, which can be used for quality control of Jianpi Fugan Pills.

Objective Using Mental Practice as a training method to train students grasping skills of minimally invasive surgery(MIS),and analyze the change of mental practice in training grasping skills of MIS.Methods 36 students were randomly divided into mental practice training group (MPTG) ( n =12 ),conventional training group (CTG) ( n=12),control group (CG) ( n=11 ).Under the uniform appraisal standards and conditions,using different training methods to teach the grasping skills of MIS,and analyze the results statistically.Results MPTG' s pre-training test results ( 16.18 ± 5.73 ) and post-training test results (79.47 ± 22.05 ) were significantly different(P ＜ 0.001 ),post-training and two weeks later test results ( 89.32 ± 31.56) displayed no significant difference(P=0.283) ; CTG' s pre-training test results ( 17.47 ±8.33) and post-training test results (57.18± 21.83 ) were significantly different (P ＜ 0.001 ),post-training and two weeks later test results (42.27 ± 24.64)had significant difference(P=0.048) ; CG's pre-training test results ( 18.19 ± 4.74) and post-training test results (38.04 ± 19.13 ) were significantly different(P =0.003 ),post-training and two weeks later test results (23.71 ±15.71 ) had significant difference (P=0.034).Compared with MPTG in pre-training test,CTG (P=0.632) and CG (P =0.464) neither had significant difference,but in the post-training test,CTG (P =0.014) and CG (P ＜0.001 ) both had significant difference compared with MPTG.In the two weeks later test,CTG and CG both (P＜0.001 ) had significant difference compared with MPTG.Conclusion Mental practice training method can improve the pedormance of gasping skills of MIS significantly and better durability,improve the learning curve,easy to implement in teaching,easy to master students,it will be a new method of MIS skills training in the future.