Anti-Bacterial Agents ; adverse effects ; Azithromycin ; adverse effects ; Chemical and Drug Induced Liver Injury ; etiology ; Humans ; Infusions, Intravenous

Objective To study the role the pathogenesis of early acute lung injury (ALI) of rabbits induced by intravascular injection of endotoxin (ET) with the intervening method of Chloroquine. Methods Rabbits were randomly assigned to three groups: control group, ET group, and ET+ chloroquine group. Acute lung injury was induced by intravascular injection of ET (500μg/kg). The arterial gas analyses, leucocyte and platelet counts in peripheral blood, PLA2 activity both in serum and lung tissue, lipid peroxide (LPO) and superoxide dismutase (SOD) in lung tissue were measured. Electron microscope and light microscope were used to observe the pathological injuries in pulmonary tissue. The protective effects of chloroquine in early ALI were evaluated. Results Compared with saline controls, rabbits treated with ET displayed the early lung injuries, such as the decrease of PaO2 (P＜0.05), the decrease of leucocytes and platelets in peripheral blood, the leukocytes sequestration in lung tissue. The PLA2 activity significantly increased in ET group compared with control group and chloroquine group both in serum and pulmonary tissue. In ET group, concentration of LPO increased in lung tissue (P＜0.05), while concentration of SOD decreased (P＜0.05). Severe histopathological injuries were presented in ET group, including pulmonary edema, lung tissue haemorrhage, inflammatory cells infiltration, asphyxial membrand formation, partial pulmonary closure and emphysema.Ultrastructural changes showed both type Ⅰ and type Ⅱ epithelial cells injury in ET group, the edema of endothelial cells, interalveolar septum thickening. In chloroquine group, PaO2 didn't decrease, PLA2 activities in serum and pulmonary tissue were lower than ET group (P＜0.05, P＜0.05), while the concentration of LPO in lung tissue decreased (P＜0.01) and SOD increased significantly (P＜0.01). Pathological examination showed slight pulmonary edema, inflammatory cells infiltration were extenuated, ultrastructural examination proved that the injuries were alleviated by chloroquine compared with ET group. Conclusion Intravascular injection of ET could successfully induce the early ALI models in rabbits. Chloroquine could inhibit the PLA2 activation and reduce the oxidative injury in lung tissue. The experiment result demonstrated PLA2 activation and oxidative stress played important roles in the pathophysiological process of early ET-induced ALI in rabbits.

Objective To study the changes in activity of phospholipase A2 (PLA2) in the course of endotoxin (ET) induced acute lung injury (ALI) inrabbits and the antagonizing effect of fructose-1, 6-diphosphate (FDP), in order to evaluate the therapeutic effect of FDP on ET-induced ALI. Methods Flapeared white rabbits were randomly assigned to three groups: control group (group A), ET challenge group (group B) and treatment group (ET challenged followedby FDP, group C). Group A animals were injected with saline (2ml/kg) as control. Group B animals were injected with ET (500μg/kg) solution followed by saline. Total amount of liquid was 2ml/kg. Group C animals were given the same amount of ET solution followed by injection of FDP (300mg/kg) solution. Total amount of liquid was also 2 ml/kg. During the experiment, respiratory rate, heart rate, blood pressure, arterial blood gases and the plasma PLA2 activity were determined at 0h, 0. 5h, 2h, 4h and 6h respectively. The rabbits were sacrificed at 6h, pulmonary PLA2 activity was assessed, and the pathologic changes in pulmonary tissues were examined with light microscope and transmission electron microscope. Results Compared with group A,rabbits of group B manifested the typical characters of ALI after ET injection, and the PLA2 activity in both serum and pulmonary tissue was much higher than those of group A (P＜0. 01).Values of the PLA2 activity in group C were between those of the two former groups. At the rame time, obvious pathological changes indicating lung injury were observed in group B and only mild pathological changes could be discerned in group C. Conclusion Activation of PLA2 activity is an important factor in pathogenesis of ET-induced ALI. FDP can antagonize the PLA2 activity and protect rabbits from early ET induced ALI to a certain extent.

Acupuncture Points ; Acupuncture Therapy ; methods ; Adult ; Female ; Humans ; Male ; Middle Aged ; Xanthomatosis ; therapy

Objective To study the changes in activity of phospholipase A_2 (PLA_2) in the course of endotoxin (ET)-induced acute lung injury (ALI) in rabbits and the antagonizing effect of fructose-1,6-diphosphate (FDP), in order to evaluate the therapeutic effect of FDP on ET-induced ALI. Methods Flap-eared white rabbits were randomly assigned to three groups: control group (group A), ET challenge group (group B) and treatment group (ET challenged followed by FDP, group C). Group A animals were injected with saline (2ml/kg) as control. Group B animals were injected with ET (500?g/kg) solution followed by saline. Total amount of liquid was 2ml/kg. Group C animals were given the same amount of ET solution followed by injection of FDP (300mg/kg) solution. Total amount of liquid was also 2 ml/kg. During the experiment, respiratory rate, heart rate, blood pressure, arterial blood gases and the plasma PLA_2 activity were determined at 0h, 0.5h, 2h, 4h and 6h respectively. The rabbits were sacrificed at 6h, pulmonary PLA_2 activity was assessed, and the pathologic changes in pulmonary tissues were examined with light microscope and transmission electron microscope. Results Compared with group A, rabbits of group B manifested the typical characters of ALI after ET injection, and the PLA_2 activity in both serum and pulmonary tissue was much higher than those of group A (P

Objective To evaluate and recommend the use of appendix anastomosed to umbilicus as the outlet of urinary reservoir. Methods The procedure has been conducted in 17 cases underwent total cystectomy from Jan 1995 to May 2000 and the outcome has been studied. Results Continence has been achieved in all.Neither difficulty in catheterization nor acute pyelonephritis was noted.There has been slight bilateral hydronephrosis in 2 patients. Conclusions The use of appendix anastomosed to umbilicus as the outlet of continent urinary reservoir is a good alternative for urinary diversion.

Objective To study the roles of phospholipase A 2 (PLA 2) and oxidative stress in the pathogenesis of acute lung injury(ALI) of rabbits induced by intravascular injection of endotoxin (ET), as well as the protective effects of chloroquine. Methods Rabbits were randomly assigned to three groups: control group, ET group, and ET+chloroquine group(n=8). Acute lung injury was induced by intravascular injection of ET (500?g/kg). The arterial blood gas analysis and serum PLA 2 activity were measured before and after the ET challenge. At the end of the experiment, PLA 2 activity, content of malodialdchyde (MDA), and superoxide dismutase (SOD) in lung tissue were assayed. Electron microscope and light microscope were used to observe the pathological injuries in the pulmonary tissue. Results Compared with saline controls, rabbits treated with ET manifested signs of ALI, such as the decrease of PaO 2(P

BACKGROUND: Fructose-1, 6-diphosphate (FDP) is an intermediate product in the course of cellular glycometabolism, and has many biological effects, such as improving cellular energy metabolism, stabilizing biological membrane, inhibiting inflammation intermedium release, antagonizing oxidation, and so on.OBJECTIVE: To observe the changes of oxidative stress reaction in the course of endotoxin (ET) induced acute lung injury (ALI) in flap-eared white rabbits and the antagonistic action of FDP, and to explore the therapeutic effects of FDP on ALI.DESIGN: Complete randomized group design, controlled experiment.SETTING: Department of Respiratory Medicine, General Hospital of Chinese PLA. MATERIALS: The experiment was conducted in the laboratory of Department of Respiratory Medicine, General Hospital of Chinese PLA between May and December 2003. Twenty-four cleaning male flap-eared white rabbits were selected and randomly assigned into control group, injured group and interventional group with 8 animals in each group.METHODS: ①Control group: The rabbits were injected with saline (2 mL/kg) through vein. Injured group: The rabbits were injected with ET (500 μg/kg) solution dissolved in 2 mL saline through cannulation in cervical vein once within 5 minutes, and then saline was injected once (Total amount of the solution was 2 mL/kg). Interventional group: The rabbits were injected with the same amounts of ET solution within 5 minutes as that in the injured group, but FDP (300 mg/kg) solution was injected later (Total amount of solution was also 2 ml/kg). ②Peripheral blood and arterial blood were collected at 0, 0.5, 2, 4 and 6 hours in each group. Peripheral blood cell count and arterial blood gas were measured with autoblood cell analyzer and blood gas analyzer, respectively. After rabbits were sacrificed through bloodletting at the 6th hour, content of lipid peroxide (LPO) and activity of superoxide dismutase (SOD) in the pulmonary tissues of rabbits were measured with barbituric acid colorimetry and pyrogallol auto-oxidizing suppression, respectively. In addition, some right lower lobes of pulmonary tissues were observed on pathology with transmission electron microscope (TEM). ③Data accorded with normal distribution and homogeneity of variance were compared by t-test, or by rank sum test.MAIN OUTCOME MEASURES: ① Comparison of result of arterial blood gas and blood cell count measured before experiment, after 0.5, 2, 4 and 6 hours experiment in rabbits of each group. ②Rabbits were sacrificed after 6 hours experiment, the pulmonary contents of LPO and the activities of SOD were measured, and the pathologic changes were also examined.RESULTS: ①At 0.5 hour after ET injected, arterial blood oxygen pressure and white blood cell count in peripheral blood were lower significantly in the rabbits of the injured group as compared with those of the control group (t=-4.27, P ＜ 0.01,z=2.64, P ＜ 0.01 ). Arterial blood oxygen pressure and white blood cell count in peripheral blood in the interventional group showed insignificant difference as compared with the control group (P ＞ 0.05). The arterial blood oxygen pressure in the interventional group was obviously higher than that in the injured group (t=4.32,P ＜ 0.01 ). ②At the 4th hour after experiment, the arterial blood oxygen pressure in the interventional group was distinctly higher than those of the control group and the injured group (t=4.98, 2.40, P ＜ 0.01, 0.05). The white blood cell count in the injured group was dramatically less than those of the control group (z=2.42,P ＜ 0.05). ③At the 6th hours after experiment, the arterial blood oxygen pressure in the interventional group was markedly higher than those of the control group (t=3.39,P ＜ 0.01), The white blood cell count in the injured group was remarkably less than those of the control group (z=2.16,P ＜ 0.05). Content of pulmonary LPO in the injured group was significantly higher than that in the control group (t=3.70,P ＜ 0.01)while the activity of SOD was obviously lower than that in the control group(t=-4.12,P<0.01).Markedly pathological lesion appeared in the pulmonary tissue. Six hours later, the content of pulmonary LPO and the activity of pulmonary SOD in the interventional group had no remarkable difference from those in the control group (P ＞ 0.05), and the pulmonary pathologic injury showed less obvious.CONCLUSION: The relative deficiency of oxidative stress reaction can aggravate oxidative injury, which is an important role in pathogenesis of ET-induced ALI. FDP can improve abilities of oxidative stress reaction, inhibit oxidative injury and protect rabbits from ET-induced ALI in flapeared rabbits to a certain extent.

China ; Dental Auxiliaries ; organization & administration ; trends ; Humans

Animals ; Corticosterone ; blood ; Male ; Rats ; Rats, Wistar ; Stress, Physiological