Objective To investigate the pathological changes of ultrastructure of mucosa in various bronchial segments from type 2 diabetic patients. Methods Sixteen cases of type 2 diabetic patients were selected,2-3 pieces of bronchial mucosa and submucosal tissue of the lesion were taken from various bronchi during bronchoscopy and these samples were observed under a transmission electron microscope. Results The basal lamina of bronchial capillary were diffusely thickened and mostly showed onion-skin like change,protein deposited around and mixed with basal menbrance;irregular highly electron dense materials were found to deposite around capillary,capillary lumen became narrow or even collapsed,neutrophilic leucocyte marginated in lumen and adhered with endothelium;protein deposited in the interstitial;endothelial cells and pericytes had dark cell changes.The cistern of rough endoplasmic reticulum dilated and vesicle formed.Conclusion Bronchial mucosa and its surrounding tissues show characteristic pathological changes of diabetes,bronchial is also the target organ of chronic diabetic damage.

The clinical medical work is accompanied with high risk .The medical treatment risk exists everywhere at anytime, but the graduate students of the Medical colleges lack the understanding of the hygiene laws. Therefore it is necessary for them to accept the education of hygiene law.

Objective To investigate the significance of serum krebs Yon den lungen-6(KL-6), surfactant protein A(SP-A), surfactant protein D(SP-D )and matrix metalloprotease 7(MMP-7) in the diagnosis of idiopathic pul-monary fibrosis (IPF) and its relationship with pulmonary function .Methods Serum levels of KL-6, SP-D, SP-A and MMP-7 were detected by ELISA in 38 patients with IPF and serum from 38 patients with pneumonia and 38 normal controls.Serum levels of markers were further analyzed by operating characteristic curve receiver (ROC), from which the ideal level of each serum marker was obtained , and the follow-up of 6 months in patients with IPF . Explore the significance of the diagnosis of IPF and its correlation with pulmonary function .Results The expres-sion of MMP-7, SP-A, SP-D and KL-6 in serum of IPF patients was higher than that in the control group and healthy control group ( P<0.05) .The optimal cutoff value of KL-6 was 723.0 U/ml, SP-A was 67.2 ng/ml, SP-D was 98.1 ng/ml, and MMP-7 was 10.1 ng/ml.The sensitivity of KL-6 was 71.1% and 98.7%, SP-A was 81.6%and 97.4%, SP-D was 81.6%and 97.4%, MMP-7 was 68.4%and 98.7%.The sensitivity of combined KL-6, SP-A, SP-D and MMP-7 four serum markers was 100%, compared with a single serum index , the P value was less than 0.05, with statistical significance .IPF patients serum SP-A, MMP-7 and forced vital capacity within 6 months were significantly correlated(r1 =-0.574,P1 <0.001;r2 =-0.465,P2 =0.003).Conclusion KL-6, SP-A, SP-D and MMP-7 were highly expressed in serum of patients with IPF , and in the diagnosis of IPF , com-bined detection of 4 serum markers can improve the early diagnosis of IPF patients .Combined serum SP-A and MMP-7 levels can improve the accuracy of the IPF patients with pulmonary function changes , the prediction of dis-ease progression has a certain significance .

Animals ; Bone Density ; Diagnosis, Differential ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Medicine, Chinese Traditional ; Osteoporosis ; drug therapy ; etiology ; metabolism ; Ovariectomy ; Phytotherapy

BACKGROUND:Corneal stromal fibroblasts have been shown to express interleukin-8 in the stimulation of lipopolysaccharide. Different reactions of fibroblasts to lipoprotein or other inflammatory mediators may constitute different characteristics of different tissues in the inflammatory response. OBJECTIVE:To observe the inhibitory effect of IKK-2 receptor blocker TPCA-1 on human corneal stromal fibroblasts to secrete inflammatory cytokines, chemokines and cel adhesion molecules under the stimulation of lipopolysaccharide and its signal transduction pathway, to compare with dexamethasone, and to explore alternative or synergistic effects after their combination. METHODS:This study measured the secretion of interleukin-1, tumor necrosis factor alpha, interleukin-6, and interleukin-8 from cultured human corneal stromal fibroblasts under the action of basic state and lipopolysaccharide, and their changes after the intervention with IKK-2 receptor blocker TPCA-1 and dexamethasone. We also detected expression level of intercelular adhesion molecule-1 and interleukin-6 in cel surface, and verified the changes in expression levels of intercelular adhesion molecule-1, interleukin-6, and interleukin-8 from mRNA level, as wel as examined the expression of nuclear factor kappa B under above conditions.

Objective To investigate the changes of the levels of high sensitivity reactive protein C (hsCRP), white blood cell (WBC) and fibrinogen (FIB) in type 2 diabetic patients with metabolic syndrom (MS). Method 323 inpatients with type 2 diabetes were divided into MS group and non-MS group according to their clinical dates, and the changes of the levels of hsCRP, WBC and FIB in the two groups and the relationship between their levels and MS were analyzed. Results Compared to non-MS group, the levels of hsCRP, WBC, FIB in MS group were increased obviously (P

Objective To investigate the expression and significance of Toll-like receptor 4(TLR4)in the uvea of endotoxin in- duced uveitis.Design Experimental study.Participants 12 Wistar rats were divided into two groups at random,a group(n=6)of endo- toxin induced uveitis(EIU)and a control group(n=6).Methods Cholera vibrio was injected into hind footpad and abdominal cavity in the EIU group.In the control group,cholera vibrio was replaced by PBS.Anterior segment of the rats eyes were observed by slit lamp microscope at 4h,10h,16h,24h after injection.Immunohistochemical staining was performed using polyclonal antibodies to TLR4 on the frozen sections of the eyes and uveal wholemounts at 24 hours after LPS injection.Main Outcome Measures The inflammation of anterior segment and the number of TLR4 positive cells in uveal wholemounts.Results The inflammation began at 4h after LPS injec- tion.Cellulose exudates were observed at 16h after injection.The positive-stained protein located in cellular membrane or cytoplasm. The number of TLR4 positive cells in EIU group is evidently higher than that in the control group.TLR4 positive cells were hardly de- tected in the frozen sections of eyes.Conclusion TLR4 expression increases in the EIU,which suggests that TLR4 probably participates in the formation and development of acute anterior uveitis.(Ophthalmol CHN,2008,17:48-51)

Objective To assess clinical safety and effect of intracoronary transplantation of autologous bone marrow cells in patients with acute myocardial infarction(AMI).Methods Eighty four AMI patients who had received emergency thromblysis or primary PTCA were enrolled in this study.Elective PCI was undergone in these patients 10-14 days after infarction.During the procedure,50 patients received introcoronary transplantation of autologous bone marrow derived mononuclear cells and the other 34 patients received normal saline as control.All patients achieved TIMI Ⅲ flow after PCI.Dobutamin stress echocardiography,SPECT and F-18-Fluorodeoxyglucose-PET were performed 1 day before and 6 months after the transplantation.All patients finished a 2-year follow up and stress echo examination.Twenty nine patients from the transplantation group and 22 patients from the control group accepted 6-month SPECT reassessment.Results No major adverse events were recorded in all patients who received autologous bone marrow cells transplantation during follow up.Less nitroglycerin usage and increased excercise were observed in the transplantation group.Stress echocardiography showed improvement in LVEF(27.00%?0.89% pre-operation,36.80%?0.58% after 6 months and 40.94%?0.58% after 2 years,P

Aged ; Coronary Disease ; Coronary Sinus ; pathology ; Female ; Heart Atria ; pathology ; Humans ; Middle Aged

ObjectiveTo explore the influence factors of prognosis of spinal cord injury patients. MethodsFollow-up data of 121 cases with spinal cord injury was analysed with retrospective cohort study.ResultsThe highest recover rate was in the patients who accepted the special rehabilitation therapy,while that of recovering two class was in the patients who accepted the magnetic stimulation.Conclusions Special recovering cure and magnetic stimulation can surely promote recovery of spinal cord injury patients.