A study on ariboflavinosis was made among the students of a college in Chongqing from Jan. to Apr. 1980. The incidence of ariboflavinosis was 18.3%. There were typical oro-genital syndromes, e.g. cheilosis, angular stomatitis, glossitis and scrotal dermatitis etc. 84 students suffered fro-m 3 types of scrotal dermatitis, The incidences of squamo-erythematoid pa-puloid and eczematoid type were 7.26%, 19% and 8.3% respectively. A patient with scrotal dermatitis(eczematoid type) was accompanied with similar lesions on the skin of buttock, right chest and right upper arm and they subsided almost at the same time with scrotal lesion after riboflavin treatment.Ten subjects of scrotal dermatitis were selected for fungus examination and it gave negative findings both microscopically and culturally.Dietary survey was made in the student's dining hall and the nutrients were calculated. The amount of riboflavin averaged only 1.07 mg per capita per day, but the calculated value was probably higher than the actual intake due to improper cooking. The intakes of thiamine, niacin and ascorbic acid were adequate.The 4-hour urinary riboflavin excretions of 10 subjects following a 5mg oral load averaged 590?g.Dietary survey, biochemical test and clinical examination benefited the diagnosis of ariboflavinosis. The lack of the knowledge of nutrition and improper cooking of vegetables were perhaps the main causes of such deficiency.

To further explore the mechanism of congenital pyrimidine 5'-nuleotidase I (P5'N-I) deficiency, on the basis of purification of the protein, the molecular weight and amino acid composition were analysed by mass-spectrograph and amino-acid analyzer, microsequencing and bioinformation analysis of P5'N-I were performed after it was hydrolysed by trypsin. The results showed that three fractions were found in the purified P5'N-I and their molecular weights were 26,952.9, 55,476 and 110,938, respectively. The sequence from one of the peptide fragments was I-E-G-P-T-I-R-Q-I-E. The homologous sequence was not found after comparision with the ten-amino-acid sequence in GenBank by blast procedure. Amino acid analysis indicated that P5'N-I was composed of 18 amino acids at least, and 243 amino acid residues. In conclusion, the enzyme might be an allosteric enzyme, there might be homologous dimer or tetramer in physiological status of normal human erythrocyte, the microsequence could be designed as the probe for fishing the genes of interest. The composition of amino acid might be an important information in determination of its protein primary structure.
5'-Nucleotidase ; blood ; chemistry ; isolation & purification ; Amino Acid Sequence ; Amino Acids ; analysis ; Chromatography, High Pressure Liquid ; Electrophoresis, Polyacrylamide Gel ; Erythrocytes ; enzymology ; Humans ; Mass Spectrometry ; Molecular Weight ; Peptide Fragments ; chemistry ; Sequence Analysis, Protein

Objective To explore the effectiveness and surgical procedures of vagus nerve stimulation (VNS) therapy in patients with drug-resistant epilepsy.Methods Fourteen patients with drug-resistant epilepsy,admitted to our hospital from January 2007 to January 2011,including 6 with epilepsy after cephalitis,3 with epilepsy after head trauma and 5 with idiopathic epilepsy,were chosen in our study; their clinical data were retrospectively analyzed.VNS was performed in all the patients; the initial programming settings were as follows:output current 0.25 mA,frequency 30 Hz,pluse width 500 μs,ON 30 s and OFF 5 min.The optimum settings for each patient should balance the goals of maximizing efficacy,minimizing side effects,and preserving better life.Results The patients experienced a median seizure frequency reduction of 63.6％ after 6-44 months of VNS therapy,including 3 patients having ＜50％ seizure reduction,11 (78.6％) having ＞or=50％ seizure reduction,6 (42.3％)having ＞80％ seizure reduction,and 2 (14.3％) having seizure free.Seizure termination or seizure diminution was reported in 5 (35.7％) patients after using the magnet.Conclusion VNS is a safe and effective therapy and could improve the quality of life in patients with drug-resistant epilepsy; VNS is also effective for patients with symptomatic generalized epilepsy.