1.Contrast-enhanced Digital Subtraction MRI in Vertebral Metastases
Wei LIN ; Hong REN ; Ming ZHANG
Journal of Practical Radiology 2001;0(01):-
Objective To evaluate the clinical application of contrast-enhanced digital subtraction MRI in vertebral metastases.Methods 50 patients with vertebral metastases were scanned by contrast-enhanced T_1WI with an injection of Gd-DTPA(0.1 mmol/kg).Digital subtraction were performed between post-contrast-enhanced images and pre-contrast-enhanced images.Vertebral metastases were evaluated by means of a contrast to noise ratio(CNR) and signal to noise ratio(SNR),and boundaries were compared between subtraction MRI images and conventional contrast T_1WI MR images.Results Contrast-enhanced digital subtraction MRI images were better than conventional contrast T_1WI MR images in showing the lesions. All lesions had a higher CNR and SNR on subtraction MR imaging than that on conventional contrast T_1WI MR images.CNR was 155.05 ? 24.73(mean ? standard error) for subtraction MR imaging and11.11?14.26 for conventional contrast T_1WI MR images,(t=35.65,P
2.Screening for chromosomal abnormalities with three maternal serum markers
Linhua LIN ; Jinghui REN ; Hong YUAN
Journal of Chinese Physician 2000;0(11):-
0.05).But the detection rates of them were all higher than that of AFP alone(P
4.Estrogen receptor ? (ER?) gene polymorphism of perimenopausal syndrome patients with liver qi stagnation syndrome (LQSS)
Hong LI ; Lin REN ; Candong LI ; Xuejuan LIN
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(06):-
Objective:To investigate the association between polymorphisms of estrogen receptor ? (ER?) gene (rs3020444) and perimenopausal syndrome (PS) in patients with liver qi stagnation syndrome (LQSS). Metheds:The ER?T/C genotype in 100 patients of PS of LQSS type (PS-LQSS group),86 patients of PS of non-LSDS type (PS-non-LQSS group) and 100 healthy subjects (control) was determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) assay. Results:Partitions of ?2 method showed that the higher prevalence of ER?-TT genotypes was noted in PS-LQSS group (?2=8.307,P=0.004),and the higher prevalence of T alleles was noted in PS-LQSS group (?2=7.129,P=0.008). and Multinomial logistic-regression indicated that the odds ratios (OR) of the ER?-TT genotypes vs TC/CC for PS-LQSS was 2.222 (95%CI:1.172-4.744,P=0.015) after adjusting for common miscellaneous factors. Conclusions:It was suggested that ER?-TT genotypes was significantly associated with PS-LQSS,and ESR?-TT may be one of the genes that contribute to PS-LQSS.
5.Clinicopathologic,immunohistochemical and molecular analysis in 15 cases of angioimmunoblastic T-cell lymphomas
Yali REN ; Lei HONG ; Lin NONG ; Shuang ZHANG ; Ting LI
Journal of Peking University(Health Sciences) 2003;0(04):-
Objective:To evaluate angioimmunoblastic T-cell lymphoma(AITL) completely, we gave in-depth investigation of histopathological features, specific immunochemical markers, antigen receptor gene rearrangements and in situ hybridization for Epstein-Barr virus (EBV). Methods: 15 cases of typical AITL displayed effacement of the normal lymph node architecture partially or completely, abundance of arborizing high endothelial vessels, infiltration of polymorphic cells and hyperplastic atypical T lymphocytes with or without clear cytoplasm. Clinical characteristics, histological manifestations, and immunohistochemical staining for CD3, CD20, CD4,CD21, CXCL13, CD10, and BCL6 were analyzed. Polymerase chain reaction for immunoglobulin heavy chain (IgH) and T cell receptor ? (TCR?) rearrangements and in situ hybridization for Epstein-Barr virus encoded RNA (EBER-1) were performed.Results: Histologically, we found eight cases with regressed lymphoid follicles, six with absence of follicles and one with hyperplastic follicles with interfollicular lesions. We also found eight cases displaying aggregation of clear cells, four infiltration of large lymphoid cells, five abundant epithelioid histiocytes. CD20 staining showed hyperplasia of large B cells in four cases. CD21 expression exihibited extrafollicular expansion of follicular dendritic cell meshworks in 11 cases (73.3%), partially with a tendency of perivascular distribution. Positive rate for CXCL13 and CD10 are 73.3% and 6.7% respectively. Monoclonal rearrangements of TCR? were detected in 6/15 (40%) of cases, IgH rearrangements in 7/15 (46.7%), of which five were monoclonal, while two oligoclonal. 8 out of 15 cases (53.3%) contained EBV-positive cells. Among the four cases with large B cell proliferation, three were EBV-positive. Conclusion: AITL display great complexity and diversity clinicopathologically. Only when we recognize such diversity, can we reasonably apply and properly evaluate immunochemical markers and molecular techniques, and thus give a correct diagnosis.
6.Related factors of death in patients with gas burst induced burn.
Lin-hong LIU ; Wen-jie REN ; Ming-jun WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(10):611-613
Adult
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Burns
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etiology
;
mortality
;
China
;
epidemiology
;
Coal Mining
;
Explosions
;
Female
;
Gases
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Humans
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Male
;
Middle Aged
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Retrospective Studies
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Young Adult
7.Clinical analysis of 221 cases of inhalation injury induced by gas explosion.
Lin-hong LIU ; Wen-jie REN ; Ming-jun WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(8):494-495
Adolescent
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Adult
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Burns, Inhalation
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etiology
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therapy
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Explosions
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Female
;
Gases
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Humans
;
Male
;
Retrospective Studies
;
Young Adult
8.Expression of survivin E-cadherin and Ki-67 in pituitary adenoma and correlation with invasiveness.
Chun-Liang WANG ; Jin-Hong MEI ; Hong-Ping WAN ; Ren-Sheng CHEN ; Lin-Lin XU
Chinese Journal of Pathology 2008;37(5):335-336
Adolescent
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Adult
;
Aged
;
Cadherins
;
genetics
;
metabolism
;
physiology
;
Female
;
Humans
;
Inhibitor of Apoptosis Proteins
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Ki-67 Antigen
;
genetics
;
metabolism
;
Male
;
Microtubule-Associated Proteins
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genetics
;
metabolism
;
physiology
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Middle Aged
;
Neoplasm Invasiveness
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physiopathology
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Pituitary Neoplasms
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pathology
;
physiopathology
;
Young Adult
9.Optimization of extraction technology of compatibility of couplet medicines Epimedium brevicornu and Ligusticum chuanxiong by orthogonal design.
Hong REN ; Xuan WANG ; Lin ZHANG ; Jing XING ; Ru-wei WANG ; Hua YIN
China Journal of Chinese Materia Medica 2015;40(9):1727-1731
The study was using the orthogonal test and making the extraction rates of icariin, ferulic acid, epimedin A, epimedin B, epimedin C, baohuoside I and ligustilide determinated by HPLC multiwavelength switch, gradient elution and multi-index comprehensive weighted scoring method (weight coefficient was 0.47: 0.16: 0.07: 0.07: 0.08: 0.06: 0.09) as evaluation index, combine with SPSS 16.0 software to optimizing the best extraction. It was Yinpian soak 1 h, 12 times more than the volumn of 50% ethanol solution, by heating reflux extraction for 60 min. The compliance test indicates that the optimized compatibility extraction technology is stable and practical, and it has provided an experimental basis for compound preparation technology research of Epimedium brevicornu and Ligusticum chuanxiong.
Chemical Fractionation
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methods
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Chemistry, Pharmaceutical
;
methods
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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Epimedium
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chemistry
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Ligusticum
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chemistry
10.Through Implement Quality Management Standardization Systems, We Strengthen the Intension Development of Research Institute
Yu CHEN ; Chunxia REN ; Lin ZHOU ; Hong CHEN ; Xiaobin CHENG ; Yejun RAO ; Yueyin ZHANG
Chinese Journal of Medical Science Research Management 2012;25(5):354,359-
According to the ISO 9001 quality management standardization,we build a new model by eight principles. Through implement the standardizations,we are benefited of the specification of the management and enhance the research reputation in the institute. We are also obtained the trust of the customers and optimize the research process and enhance the achievement quality.This system is rigorous and abundant.It is specific and manipulate easily.Through implement quality management standardization systems,it can also promote the achievement to transform the productivity in a forward step.