Child ; Diagnostic Errors ; Epilepsy ; diagnosis ; Female ; Humans ; Pseudohypoparathyroidism ; diagnosis

0.05) except IP between T_4 and T_(16)(P0.05).the dynamic and static RBI showed the same change tendency.[Conclusion]The intraosseous and intramedullary blood circulation is severely damaged after blocking of the proximal and middle FMC with bone cement,it induces a long time high IP and a series of changes of blood flow at distal femur.

[Objective]To explore the effects on the distal femoral joint by high intraosseous pressure after blocking of proximal and middle femoral medullary cavity with bone cement.[Method]The rabbit model was established whose proximal and middle femoral medullary cavity of left side was infused with PMMA while the right side served as the control.Twenty-six rabbits were cavided randomly into 4 groups,8 for normal control group,the remained modeled rabbits were grouped according to the observing time-point into following groups:4th(T4),8th(T8) and 16th week(T16).The rabbits was killed to obtain the samples of distal femoral articular synovium,cartilage and the bone under cartilage.Then the structural changes of these tissues were observed by HE staining,toluidine blue staining,immunohistochemistry and transmission electron microscope.[Result](1)HE staining:the damage to articular cartilage,synovial tissue and the bone under cartilage aggravated with time.At the 16th week,it showed the articular cartilage was destructed,the bone structure was severely damaged with the synovial tissue proliferation and swelling.(2)Toluidine blue staining(16th week):all the layers of articular cartilage appeared loss of stain while the superficial layers were slightly loss of stain in control group.(3)Immunohistochemistry(16th week):①CollagenⅡ:the chondrocytes were positively stainned.②TGF-?1:chondrocytes stain was positive;the staining of articular synoviocytes appeared positive.(4)Transmission electron microscope:The lesions to articular chondrocytes and bone cells aggravated with time,part of which presented with necrosis and collapse at the 16th week.[Conclusion]The distal femoral intraosseous and intramedullary blood circulation is severely damaged after blocking of the proximal and middle femoral medullary cavity with bone cement,leading to continuous high intraosseous pressure.It makes the bone metabolism disordered in distal femur,which results degeneration and necrosis in distal femur,cartilage and synovial tissue.

Objective To evaluate the advantages of combined transvaginal hydrolaparoscopy (THL) and hysteroscopy in patients with infertility. Methods Twenty-three primary infertile patients (primary group) and 23 secondary infertile patients (secondary group) were received THL (using a 3-mm scope with a 0? lens) and hysteroscopy. The fallopian tube patency, pelvic pathology, operative time, discharge time, healing time of vaginal wound, success rate of insertion into the pouch of Douglas and intra-and postoperative complications were observed. Results Primary group had more tube patency cases (P

To establish a simple and reliable animal model of skin photo-damage, 20 mice were treated with 8-MOP and exposed to UVA (UVA 320-400 nm) for 24 h. After irradiation, the structure of the epidermis and dermis, collagen fibers, elastic fibers were observed by using HE staining and Weigert technique and compared with the normal controls. The acanthosis and epidermis proliferation with accompanying hyperkeratosis and parakeratosis were observed. Inflammatory infiltration was noted in the dermis. The elastic fibers became coarse, irregularly arranged and clustered, with their number increased. The collagen fibers showed obvious degeneration and some amorphous materials could also be observed. The blood vessels were irregularly dilated and vascular walls were thickened, with infiltration of inflammatory cells. It is concluded that murine photodamage model can be quickly, conveniently and reliably established by means of 8-MOP/UVA.
Dermis/pathology ; Disease Models, Animal ; Epidermis/pathology ; Methoxsalen/*pharmacology ; Photosensitizing Agents/pharmacology ; Skin/*pathology ; Skin Aging ; Ultraviolet Rays

Objective To investigate the effect of flavopiridol on the proliferation,invasiveness and apoptosis of human prostate cancer cell line LNCaP,and to explore the possibility of its application in clinical treatment.Methods MTT assay was used to detect cell proliferation,cell invasion in vitro was detected by Transwell assay,and flow cytometer was used to observe apoptosis.Results Flavopiridol inhibited the growth of LNCaP cells in a concentration-dependent and time-dependent way (P ＜ 0.05),and reduced the ability of invasion capacity.After treated by 10 nmol/L flavopiridol for 24 h,the apoptosis rate was increased significantly to (7.5±0.9) ％ compared with the control group [(5.3±0.5) ％] (P ＜ 0.05).Conclusion Flavopiridol can inhibit proliferation of LNCaP cells and induce apoptosis,which may be applicable for the treatment of prostate cancer.

To confirm the existence of heme oxygenase (HO)- carbon monoxide (CO)- cyclic guanosine monophosphate (cGMP) pathway in the cultured human trabecular meshwork cells (HT-MCs) in vitro, and to evaluate the inductive role of hemin on this pathway, HTMCs of the third to fourth generation were cultured in vitro. Reverse transcripase-polymerase chain reaction (RT-PCR) was employed for detection of HO-1 and HO-2 mRNA. Immunohistochemical staining was used to detect HO-1 and HO-2 proteins. Hemin was added into the culture solution. The HO-1 mRNA levels were quantified by RT-PCR. The relative amount of carbon monoxide released into the media was measured with the quantifying carbon monoxide hemoglobin (HbCO) by spectrophotometry. Radioimmunoassay was used to determine changes of cGMP in HTMCs. The results showed that cultured cells had the specific characteristics of HTMCs. Both HO-1 and HO-2 genes were expressed in HTMCs, as well as HO-1 and HO-2 proteins in HTMCs. Hemin induced HO-1 mRNA, HbCO and cGMP in a dose-dependent manner. In conclusion, HO-CO-cGMP pathway exists in the cultured HTMCs and can be induced by hemin. Pharmacological stimulation of HO-CO-cGMP pathway may constitute a novel therapeutic approach to rescuing glaucoma.
Carbon Monoxide/*metabolism ; Cells, Cultured ; Cyclic GMP/*biosynthesis ; Cyclic GMP/genetics ; Heme Oxygenase (Decyclizing)/*biosynthesis ; Heme Oxygenase (Decyclizing)/genetics ; RNA, Messenger/biosynthesis ; RNA, Messenger/genetics ; Signal Transduction ; Trabecular Meshwork/cytology ; Trabecular Meshwork/*metabolism

ObjectiveTo evaluate the effect of Yuxingchangzhi Decoction on level of norepinephrine (NE) and 5-hydroxyptamin (5-HT) in serum of patients with post-stroke depression. Methods76 patients with post-stroke depression were treated with Yuxingchangzhi Decoction or placebo for 4 weeks. Before and after treatment, all the patients were evaluated with the Hamilton Rating Scale for Depression (HAMD),while the level of NE and 5-HT in serum of patients were measured.ResultsThe total score of HAMD of the patients of Yuxingchangzhi Decoction group was significantly different before and after treatment (P<0.01),while that of the placebo group was not significantly different (P>0.05). The NE and 5-HT level in serum of patients of the Yuxingchangzhi Decoction group were significantly different before and after treatment (P<0.01),but that of the placebo group was not significantly different (P>0.05). ConclusionYuxingchangzhi decoction can raise NE,5-HT level in patients with post-stroke depression and alleviate symptoms of depression significantly.

Objective To evaluate the anatomic outcome after lens-sparing vitrectomy (LSV) or scleral buckle (SB) for stage 4 retinopathy of prematurity (ROP).Methods The clinical data of 39 infants (50 eyes) with 4a (20 eyes) or 4b (30 eyes) were retrospectively analyzed.The age ranged from two to 18 months,with a mean of (6.0±3.4) months.The gestational age ranged from 26 to 33 weeks,with a mean of (30.0±1.6) weeks.The birth weight ranged from 800 to 1900 g,with a mean of (1404.5±237.6) g.Nineteen eyes underwent SB and 31 eyes underwent LSV.Follow-up ranged from 6 to 84 months,with a mean of (26.0±21.7) months.The anatomical and refractive results were reviewed at the final follow-up.Results The anatomic success of SB was 100.0％ (19 of 19 eyes) and that of LSV was 87.1％ (27 of 31eyes).Among the patients in whom treatment failed,4 were in the LSV group (4/31,12.9％).The buckles of 5 eyes (5/19,26.3％) were removed.At the end of the follow-up,the mean myopic refraction was (-4.46±2.49) diopters (ranging from -1.25 to 11.00 diopters) in the LSV group,and ( -3.21±1.96) diopters (ranging from -1.25 to 9.25 diopters) in the SB group.There was no significant difference between two groups (F=2.76,P=0.103).Conclusion The anatomic outcome after LSV or SB for stage 4 ROP was excellent.