BACKGROUND:During in vitro culture, dental folicle cels are easy to loss self-renewal capacity and become aging, and they are also very difficult to be purified and amplified in quantity, which limits the application of dental folicle cels in periodontal tissue engineering. OBJECTIVE:To study the osteogenic effect of immortalized rat dental folicle cels. METHODS:pSSR69-pAmpho plasmids containing SV40T-Ag were used to transfect 293 cels. Rat dental folicle cels were transfected with virus supernatant and screened by hygromycin to establish immortalized rat dental folicle cels (experimental group). Untransfected cels served as controls. RT-PCR was used to detect the osteogenic related factors (alkaline phosphatase, osteocalcin, bone morphogenetic protein 2, and Runx2) in the experimental group and control group. RESULTS AND CONCLUSION:The results showed no statistical differences between the experimental group and the control group in the expressions of alkaline phosphatase, bone morphogenetic protein 2 and Runx2 (P > 0.05). The expression of osteocalcin was significantly higher in the experimental group than the control group (P < 0.05). These findings suggest that in the late osteogenesis differentiation, immortalized rat dental folicle cels may promote the secretion of osteocalcin and then make osteoblasts early entry into the bone calcification stage.

Objective To investigate the myocardial remodeling and cardiac function effects of metoprolol combined Xinshuaining mixture for treating chronic heart failure.Methods One hundred and seventy patients with chronic heart failure was equally divided into treatment group and control group of 85 cases each,accorded to the random principle of parallelism,both groups were given conventional treatment based on metoprolol,the treatment group was added Xinshuaining mixture.Results After treatment,the effective rate in the treatment group was 95.3 ％(81/85),so that was 81.2％(69/85) in the control group,there was significant difference (P ＜ 0.05).After treatment,the level of left ventricular end-systolic volume (LVESV),left ventricular end-diastolic volume (LVEDV),heart rate (HR) in treatment group were significantly lower than those in control group [(41.36 ±7.11) ml/m2 vs.(44.32 ±6.26) ml/m2,(46.28 ± 7.56) ml/m2 vs.(55.69 ± 8.12) ml/m2,(72.25 ± 3.62) bpm vs.(78.36 ± 3.44) bpm],while the level of left ventricular ejection fraction (LVEF) and 6-minute walk test distance were significantly higher [(56.48 ±8.00)％ vs.(35.16 ± 8.21)％,(566.68 ±51.86) m vs.(476.88 ±50.99) m],there were significant differeces (P ＜ 0.05).After followed up for 3 months,the incidence of cardiac adverse reactions in treatment group was significantly lower than that in control group [3.5％ (3/85)vs.11.8％ (10/85)] (P ＜ 0.05).Conclusions Metoprolol combined Xinshuaining mixture for treating chronic heart failure can improve cardiac remodeling and heart function,thereby improve the prognosis of efficacy.While its better security should be widely applied.

From October 9 to 12,2008,the 2th Academic Meeting of the Basic Theory of TCM Branch was held in Huangshan,Anhui province.The theme of this meeting was to discuss the key scientiflc problems of Zang xiang and Zheng Hou.The meeting arranged special topic reports and free speeches.The aims of the meeting were discussing key sclentific issues in the science of basic theory of TCM,exchanging experiences and new technologies and methods,promoting the establishment and construction of academic team,and advancing the development of this science.

Humans ; Voice ; Voice Quality

Liver transplantation is a valid treatment option for select patients with Hepatocellular Carcinoma HCC and end-stage liver disease. However, in approximately 20% of patients, recurrent HCC is the rate-limiting factor for longterm survival. Despite identification of clinical parameters that may stratify patients at high risk and exhaustive preoperative staging, cancer recurrence is likely the result of microscopic extrahepatic disease. With a desperate donor organ shortage, locoregional ablation techniques and resection are being employed in patients on the waiting list to serve as a bridge to OLT. Further more, some have advocated aggressive surgical resection of isolated metastasis in both the liver and extrahepatic viscera. Whether these creative strategies confer a survival advantage is unknown, requiring longterm follow-up to determine their efficacy.

Objective To investigate the technique and outcome of primary management of skull base fracture and cerebrospinal fluid (CSF) leakage by emergency surgery in patients with severe craniocerebral trauma. Methods A total of 16 patients with severe brain injury, skull base fracture and CSF leakage hospitalized in Department of Neurosurgery, Tianjin Medical University General Hospital from January 2014 to December 2015 were retrospectively analyzed. All of patients underwent emergency surgery to remove the hematoma and repair the skull base fracture, including anterior skull base repair in 12 patients and middle temporal skull base repair in 4 patients. During the surgery, the inner wall of the frontal sinus was managed by electrocautery, then removal of frontal sinus mucosa, hydrogen peroxide and iodophor rinse were used. The repairing and forming of the orbital roof were performed for the severe deformation cases. All bone fractures and fissures were filled with muscle blocks and biological glue and covered with a pedicled muscle flap. In addition, after close suturing of epidural, the intact periosteum under the skin flap was used to tile the skull base. No external material was used in the whole process. Results Among the 16 patients, 14 (87.5%) patients were successfully repaired by emergency surgery, and 2 cases (12.5%) failed. One of the patients who failed to repair the fracture was with the middle skull base fracture, severe fracture of skull base and mastoid, and extensive exposure of mastoid air chamber with large bone mass. This patient failed secondary surgical repair and died with severe infection. Another one case who failed primary surgery was with severe bone fractures in the anterior, middle and posterior skull base, which could not be completely repaired. This patient was also combined with infection and dead ultimately. Conclusion Primary repair in patient with severe head injury combined with skull base fracture, which needs surgical intervention can reduce further injury and save the patient's life. The key to the operation is to determine the operative principle and to repair the skull base tightly.

Objective To explore the effects of vecuronium bromide within 24 hours in severe bronchial asthma with acute attack with breathing machine in conventional sedatio.Methods Prospective study,double-blind randomized standard control,where 46 cases of patients of severe bronchial asthma with acute attack transferred to ICU using breathing machine in this year were randomly divided into the vecuronium bromide group (muscle relaxant group) and conventional sedation group (normal group) according to medical record number; Two groups of sample compositions were compared by x test,using two independent samples of t test to compare the effects the treatment and prognosis.Results After mechanical ventilation for 15min and 1-3h in muscle relaxant group,patients' vital signs were statistically analyzed,including respiration; Blood gas analysis parameters were as follows:pH,PaO2,PaCO2,SpO2 of these were improved compared with those in the conventional group (P ＜ 0.05) with statistically significant differences;Compared with the conventional group,in the muscle relaxant group,the retention time in ICU (146.20 ± 17.92) hours,(214.15 ± 22.21) hours,the using time of breathing machine (125.93 ± 16.23) hours,(192.89 ± 22.60) hours,and the dosage of glucocorticoid (2.61 ± 0.17) mg · kg-1 · d-1,(3.55 ± 0.26) mg · kg-1 · d-1,anticholinergic agent(6.25 ±0.51) μg · kg-1 · d-1,(10.64 ±0.75) μg · kg-1 · d-1 and β2 adrenergic receptor agonist (0.06 ±0.01)mg · kg-1 · d-1,(0.15 ±0.04)mg · kg-1 · d-1 were all reduced (all P ＜0.05) with statistically significant differences;The patients were smoothly transferred out of ICU,without the adverse events of mechanical ventilation and cardiovascular diseases.Conclusion The use of vecuronium bromide made the patients with severe bronchial asthma in routine sedation use of breathing machine rapidly correct the clinical symptoms and improve the off-period prognosis.

Objective To analyse the expression and clinical significance of 12 kinds of microRNAs (miR) in patients with ovarian cancer using public gene expression databases. Methods The microRNA expression data were screened in dataset GSE14407 and TCGA database, then 12 kinds of microRNAs were obtained including miR-10B, miR-1244, miR-622, miR-21, miR-503, Let-7D, miR-155, miR-30C, miR-17, miR-101-1, miR-186 and miR-770. The expression data of these 12 kinds of microRNAs were compared and identified to find the differential ones between normal tissue and tumors. Data of 505 ovary cancer patients were divided into two groups by age, tumor grade, clinical stage, disease location, tumor residual and microRNA expression. Kaplan-Meier survival analysis and Cox multivariate analysis were used to compare the overall survival of ovary cancer patients between two groups. Results Compared with ovary cancer, the expression levels of Let-7D and miR-101-1 were higher, but the expression levels of miR-155 and miR-770 were decreased, in adjacent tissue of ovary tumor (P<0.05). The Kaplan-Meier survival analysis result showed that lower survival rates were found in patients with age≥59 years, clinical stage (Ⅲ+Ⅳ) and lower Let-7D expression (P<0.05). The multivariate Cox regression analysis showed that the decreased expression level of Let-7D was the independent risk factor for the prognosis of ovarian cancer. Conclusion The expression of Let-7D is correlated with the prognosis of ovarian cancer, which is the independent biomarker to predict prognosis of ovarian cancer.

OBJECTIVE:To establish RP-HPLC combined with solid phase extraction method to determine the plasma concentration of lansoprazole.METHODS:The sample was extracted by solid phase extraction in darkness.RP-HPLC was applied using Venusil XBP-C18(200 mm?4.6 mm,5 ?m) column.The mobile phase consisted of acetonitrile-water(32:68,pH value adjusted to 7.6 with 1% ammonia water) and flow rate was 1.0 mL?min-1.The detection wavelength was set at 284 nm and column temperature was set at room temperature.RESULTS:The linear range of lansoprazole was 10～2 500 ng?mL-1(r=0.999 9) with mean recovery of 100.93%.The RSD of intra-day and inter-day were both less than 10%.CONCLUSION:The method is sensitive,convenient and rapid for the determination of lansoprazole concentration in plasma.

OBJECTIVE:To evaluate the status quo and the developmental trend of the application of antidepressant drugs in our hospital.METHODS:The antidepressant drugs in our hospital during2002～2004were analyzed retrospectively in terms of drug varieties,consumption quantity,consumption sum,DDDs etc.RESULTS:The consumption sum of the antidepressant drugs increased year by year,which stood at667.41,802.47and995.02(RMB:ten thousand yuan),respectively in the3years.CONCLUSION:Fluoxetine dominates the first place in terms of clinical application of antidepressants.Furthermore,the application of new type antidepresants has been on the rise.