Objective To analyze the hereditary background of common species from Rhodiola L. in Yunnan Province. Methods Fifty-nine samples of six populations in three natural medicinal species from Rhodiola L. were studied by RAPD method. Results The percentage of polymorphic bands (PPB) of three populations from R. crenulata were 37.97%, 46.15%, and 39.45% respectively; the Nei's gene diversities (h) were 0.110 3, 0.143 7, and 0.137 1, respectively; the Shannon's information indes (I) were 0.173 6, 0.222 1, and 0.206 7, respectively; at the special level, the PPB was 70.47%, I was (0.258 3); the genetic differentiation (G_(st)) was 0.263 0. The PPB of two populations from R. fastigiata were 43.67% and 44.42%, I were 0.215 3 and 0.217 4, respectively; at the special level, the PPB was 65.51%, G_(st) and I were 0.313 2 and 0.313 9, respectively; the PPB of R. yunnanensis was 36.97%, I was 0.188 1. Conclusion RAPD molecular marker could be used to molecular authentication and genetic diversity analysis of medicinal species from Rhodiola L.

Objective To investigate the application of heat-clearing and detoxifying Liangxue prescription decocting-free granules in puerperal infection and its clinical curative effect.Methods 97 cases patients with puerperal infection were chosen in the hospital from Dec.2012 to Dec.2014, according to the digital method, which were randomly divided into experimental group (n=49) and control group (n=48).The two groups were given metronidazole injection 250 mL/d, and 0.2 g mesylate levofloxacin added into 5% glucose injection 250 mL, intravenous drip, twice per day.On the basis of above therapy, experimental group were treated with heat-clearing and detoxifying Liangxue prescription decocting-free granules, orally, twice per day, one bag each time.Both of two groups were treated for 2 weeks as one course.Clinical symptom score, hemodynamic indexes, inflammatory factor, blood biochemical indexes and efficacy were observed and compared between two groups.Results Total effective rate of experimental group was 95.92%, which was significantly higher than 75.00% of control group ( U =3.58, P<0.01 ) .After treatment, the cell count of white blood cell ( WBC) and neutrophilic granulocyte ( GRAN ) , values of middle cell percentage (%MON ) , neutrophilic granulocyte ratio (%GRAN ) , and lymphocyte percentage (%LYM) in both of two groups significantly decreased compared with before treatment (P<0.05), and the above indexes in experimental group were lower than those in control group ( P<0.05 ) .After treatment, the serum IL-6 and TNF-αlevels in two groups decreased compared with before treatment ( P<0.05 ) , and the above indexes in experimental group were lower than those in control group ( P<0.05 ) .After treatment, the hemodynamic indexes including cell aggregation index, whole blood viscosity ( high, medium and low shear ) and plasma viscosity significantly improved compared with before treatment than control group ( P<0.05 ) , and the above indexes in experimental group significantly improved compared with control group (P<0.05).After treatment, the score of hypogastralgia, chills and fever, uterine body tender and a funicular tenderness of attachment area, lochia score and total score significantly decreased compared with before treatment (P<0.05), and the above indexes in experimental group significantly were lower than control group ( P<0.05 ) .Conclusion The heat-clearing and detoxifying Liangxue prescription decocting-free granules can significantly improve the inflammatory symptoms in treatment of puerperal infection, and its mechanism of action may improve microcirculation, reduce inflammatory reaction.The clinical efficacy of therapy is obviously better than western medicine treatment alone, which can be popularized in clinical application.

No abstract available.
Humans ; Hypogonadism* ; Male*

No abstract available.

No abstract available.
Growth Hormone* ; Receptors, Somatotropin*

The ras-mitogen activeted protein kinase pathway is responsible for regulation of many cel-lular processes, including differentiation, cytoskeletal organization, and protein trafficking. Much attention has been paid to them and their role in cancer therapy recently, and its inhibitor pathways are applied to clinical tes-ting, such as, Raf kinse inhibitors, MEK inhibitor, Src kinase inhibitor.

OBJECTIVE To explore the incidence of the nosocomial infection in patients with mental diseases,(analyze) the correlative factors and then reduce the incidence.(METHODS) By using the retrospective method,the(inpatient) cases in psychiatry department from Jan 2000 to Dec 2004 were surveyed and statistically analyzed.(RESULTS) There were 313 cases of nosocomial infection from 9 089 inpatients with mental diseases.The infection rate was(3.44%.) The total rate had been dropped year by year.The major sites of the infection were the(upper)(respiratory) tract(34.82%),the gastrointestinal tract(20.45%) and the urinary system(18.53%).The(infection) rate in ladies patient area(3.83%) was significantly higher than that of gentlemen patient area(3.07%)(P

Metabolic diseases information consulting provides its clients with data and insight to help translation information medicine product development plans:for clinical,diagnosis,biologics,medical devices/combination products.Understanding the mechanisms by which specific protein functions contribute to metabolic disease pathogenesis is a great challenge.The barcode is the common nominator and identifier of a sample.This code can be utilized in both 2D form,capturing important identifiers for each sample type and origin.Visual database informational system,to impact on the quality of the analysis data generated.Our ultimate motivation lies in helping you to advance the translation medicine success of our hospital,and to optimize the benefit they provide to patients in need.

Objective To observe the effect of stress caused by neuropathic pain on serum cortisol concentration and expres-sion of 11β-hydroxysteroid dehyarogenase 1(11βHSD1)and investigate the action mechanism of curcumin for alleviating neuropathic pain. Methods The 72 male SD rats were randomly divided into 4 groups with 18 rats in each group:sham operation group(Sham), chronic constrictive injury group(CCI),solvent contrast group(SC)and curcumin treated group(Cur100). Curcumin(100 mg/kg/d) was given after the operation in Cur100 group. Paw thermal withdrawal(PTWL)and paw mechanical withdrawal threshold(PMWT)of rats were measured on 2 pre-operative and 1,3,5,7,10,and 14 post-operative days. The animals were deeply anesthetized,the blood was taken from the heart,and the L4-L5 dorsal root ganglion(DRG)of the operated side was removed. The change of cortisol was measured by ELISA and the change of 11βHSD1 expression in spinal cord and DRG was determined by immunochemistry and Western blot. Results Compared with sham group,PTWL and PMWT decreased significantly after operation in CCI group(P<0.01),serum cortisol concentration was significantly increased(P<0.01)and the expression of 11βHSD1 was significantly increased in CCI group(P<0.01). Compared with CCI group,the PMWT and PTWL was increased in CCI group(P<0.05),the serum cortisol concentration was decreased(P<0.05)and the expression of 11βHSD1 was inhibited in Cur100 group(P<0.05). Conclusion Stress caused by neuropathic pain triggered the release of cortisol to the blood and increased the expression of 11βHSD1 at the same time. Cur-cumin could alleviate thermal hyperalgesia and mechanical hyperalgesia induced by CCI and could also inhibit the serum cortisol con-centration and the expression of 11βHSD1 in spinal cord and DRG,which was likely to be related to pathogenesy and maintenance of neuropathic pain.

Objective To determine CD+4CDnull28 T cells and their relationship with the microinflammatory state and left ventricular hypertrophy in end-stage renal disease (ESRD) patients.Methods One hundred and twenty ESRD patients were selected,including sixty non-dialytic stage 5 chronic kidney disease patients(CKD5 stage) and thirty maintenance dialysis patients(CKD5D stage).In addition,thirty healthy adults from the hospital medical center were selected as the control group.Flow cytometry was used to detect the frequencies of T lymphocyte subsets in peripheral blood.All enrolled subjects underwent Doppler echocardiography investigations.Results(1)Compared with the control group,the high sensitivity C-reactive protein and the frequency of CD+4CDnull28 T cells were significantly expanded in CKD5 and CKD5D paients (F=14.887,9.659,all P<0.05).(2)In ninety ESRD patients,the incidence of LVH increased in CKD5 and CKD5D patients (76.67% vs.86.67%).(3)Linear correlation in CKD5 patients showed that:LVMI was positively correlated to duration of hypertension,SBP,P3+,lg(CRP+1),lgiPTH,lg(CD+4CDnull28+1) (r=0.332,0.291,0.269,0.310,0.304,0.396,all P<0.05),as well as negatively correlated to Hb(r=-0.285,P<0.05).Multiple linear regression analysis showed that LVMI was independently associated with lg(CD+4CDnull28+1) and duration of hypertension(F=6.589,P<0.000).ConclusionThere is microinflammatory state in the ESRD patients regardless of whether or not they have dialysis treatment,and they have the high incidence of LVH and exhibit a substantially increased frequency of circulating CD+4CDnull28 T cells.The frequency of CD+4CDnull28 T cells and duration of hypertension are the independent predictors of LVH in non-dialytic stage 5 CKD patients,indicates that these cells may play a critical role in the development of cardiovascular disease.