Objective To explore the significance of detecting serum MMP-10 in type 2 diabetic nephropathy.Methods 100 diabetic patients with suspected DN were devided into two groups,that was DM(42 cases)and DN(58 cases)group,and 60 casescontrolgroup(NC).By Mogenson,DN1(30 cases)and DN2(28 cases),HbA1c,BUN,Scr,UAER,β2-MG and MMP-10 leves were detected by the standard method.Results There were obvious differences of HbA1c,BUN,Scr,UAER,β2-MG and MMP-10 levels among DN,DM and NC(P<0.05).Apart of HbA1c,others were no differences between DM group and NC group(P>0.05).DN group was higher significantly than these in DM group(P<0.05),and HbA1c,BUN,Scr,UAER, andβ2-MG levels had no statistical difference between DN1 and DN2 group.MMP-10 level in DN2 was higher than that in DN1 group(P<0.05).Conclusion MMP-10 in serum may be a role indicator for impairment of renal function for diagnosis of type 2 diabetic nephropathy.

Objective:To study the hematopoietic reconstruction for bone marrow depression mice with mobilized peripheral blood stem/proginiter cells by ginseng polysaccharide.Methods: Establish hematopoiesis failure model of mouse and transplant murine peripheral blood mononuclear cells mobilized by GPS.To observe the survival time,WBC,the weight of spleen,the number of CFU-S of recipient mice and chromosome chimerism.Results: After PBMNCs(1?105)mobilized by GPS from mice(male) were injected to the lethality irradiated mice,it can reduce the time of WBC recovery obviously;prolong the time of murine survival,increase the number of WBC of the recipient mice;it can also increase the number of CFU-S,the weight of spleen;Evaluating the Y chromosome by PCR,the result showed that 581bp Y chromosome sequence was examined in peripheral blood of the recipient mice.Conclusion: There are a lot of hematopoietic stem/ proginiter cells in peripheral blood of the mice mobilized by GPS,so it can obviously reconstruct the hematopoiesis of the bone marrow depression mice by transplantation.

Objective:To study the mobilizable effect of ginseng polysaccharide(GPS)on murine hematopoietic stem/ progenitor cells and provide experimental evidences for mobilizable preparation of hematopoietic stem cell from plant polysaccharide.Methods:The changes of the numbers of WBC and MNC,the percentage of CD34+ cell and the yields of CFU-Mix in peripheral blood were examined by means of WBC and MNC counts,immunocytochemistry,flow cytometry and hematopoietic cell culture in vitro.Results:4 hours after injection of GPS into mice with a dosage of 2 mg/kg,the numbers of WBC and MNC reached the maximum,which were 3 times and 3.5 times that of the NS group respectively.The percentage of CD34+ cell and the yields of CFU-Mix in vitro in peripheral blood(0.0079?0.0011 and(25.0?9.1)cfu/2?105MNC respectively)after the mobilization by GPS were obviously higher than those of the NS group.The effect of mobilization by combination of GPS and G-CSF was much better than that mobilized by single administration of GPS.Conclusion:GPS alone or in combination with G-CSF could significantly mobilize murine hematopoietic stem/progenitor cells from bone marrow into peripheral blood.

Surface electromyography (SEMG) is firstly used for sports study to analyze muscular movement time sequence and contribution to movement under different sports and know whether initiation and duration of each muscle during exercise training is normal. Subsequently, SEMG is used for biofeedback to increase sports selectivity and concordant and accelerate functional recovery. Recently, extensive domestic studies on SEMG are focusing on rehabilitation medicine, especially on functional evaluation during muscle strength training exercise.

Objective To standardize scrap and process management of the medical equipment. Methods The scrap of medical equipment was discussed in aspects of standard, program and process of medical equipment scrap. The management level of the medical equipment was improved and dynamic management was realized. Results The intact rate, rate of depreciation and total value of the equipment were correctly reflected. Conclusion The economic and social benefit of the hospital are increased.

Medical equipment has an important status in medical cure. The relativity in purchase and maintenance of medical equipment is discussed and many reasonable proposals in equipment service employment are put forward. The re- pairing people need to cooperate perfectly to purchase equipment and assure the quality of it, decrease the maintenance cost and improve the economic and social benefit of the hospital.

The existent questions of medical equipment assets check are discussed and gives some modified solutions such as perfection and implementation of equipment management system, implementation of bar code management, rigorous scrapped system, strengthening use, deployment and presenting of medical equipment.

To enhance the communication and collaboration between medical equipment department and clinical departments, such aspects has to be laid emphasis on as purchase, maintenance, allocation of medical equipment as well as supply and management of medical consumables.

Object To investigate the ultrafine grinding and nanometer powder's antiobesity effect of Amorphophallus rivieri Durieu on nutritional obese rats. Methods The content of triglyceride, cholesterol and high density lipoprotein (HDL) of blood was measured by UV spectraphotometry method. The blood glucose was determined by spectraphotometry method. The antiobesity effect was determined by weight loss and Lee's coefficient. Results Compared with powder and polysaccharide of A. rivieri, the ultrafine grinding and nanometer powder of A. rivieri could significantly decrease the rat body weight and the Lee's coefficient of nutritional obese rats (P

Objective　To establish a simple assay for quick scanning of effective agents in growth inhibition and apoptosis induction. Methods　After observing the correlation between cell number and acid phosphatase activity, and between cell apoptosis and acid phosphatase activity, we established a microplate densimetry method to measure the acid phosphatase activity. We also compared the sensitivity with MTT assay and confirmed cell apoptosis by flow cytometry. Results　In two cell lines of cancer (Hep G2 and CBRH-7919 cells), the acid phosphatase activity was correlated well with the cell number, and was directly proportional to the cell number in the range of 0.5×103～0.7×103. The coefficient reached to 0.994. After stimulation of phorbel ester (TPA) for one hour, the acid phosphatase activity rose significantly. In contrast, after the inhibition of cell growth by As2O3, the acid phosphatase activity decreased significantly. The higher the concentration of As2O3, the lower the acid phophatase activity. The acid phophatase activity went down significantly when apoptosis in Hep G2 cells was only 3.98% and no apoptosis in CBRH-7919 cells after 24-hour treatment with As2O3. Conclusions　The acid phophatase activity of cell may be used to measure cell proliferation and apoptosis. Because of the easiness and quickness, acid phophatase assay might be an ideal approach to scan the effective growth inhibitors and apoptosis inducers in a large number of drugs.