Objective This study was designed to assess whether Bushen Tongluo Fang(BSTLF),a Chinese materia medica formula,can ameliorate the hypercoagulable state in rats with membranous glomerulonephritis(MGN)induced by cationized bovine serum albumin(C-BSA)in order to gain an insight into the mechanisms responsible for its therapeutic effect.Methods Seventy-two male Sprague-Dawley rats were randomly separated into six groups:the normal control group,the MGN model group,the prednisone-plus-aspirin treatment group,and the low-,moderate-,and high-dose groups of BSTLF.The model of MGN was induced by sc(preimmunization)and iv injections of C-BSA in the latter five groups.After the development of MGN model,the decoctions of BSTLF and the prednisone-plus-aspirin solution were ig administered to the treatment groups respectively twice daily for four weeks.The rats in the model group received their drinking water as vehicle controls.Urinary albumin excretion for 24 h was measured using a rat albumin ELISA kit.The platelet aggregation was analyzed by turbidimetry.The plasma level of fibri-nogen(Fib)was determined by the von Clauss assay.Radioimmunoassay was used to examine thromboxane B2(TXB2)and 6-keto-prostaglandin F1?(6-keto-PGF1?)of the renal cortex.Results Urinary albumin excretion for 24 h,maximal platelet aggregation,plasma Fib level,and TXB2 production of renal cortex in the MGN model group were significantly higher than those in the normal control group,respectively.Compared with the MGN model group,these four measurements were decreased significantly in BSTLF-treated groups.The 6-keto-PGF1? production of renal cortex in the MGN model group was subnormal but no statistically significant differences were observed between the model group and BSTLF groups.Conclusion The results of this study suggest that the administration of BSTLF could attenuate platelet aggregability,lower plasma level of Fib and reduce thromboxane A2 production by renal cortex in the rats with MGN,so that their hypercoagulable state is corrected at least partly and glomerular microthroimbosis is prevented in several ways.These effects may contribute considerably to the mechanisms of BSTLF efficacy for chronic nephritis.

Objective To observe the therapeutic effect of cervical intraepithelial neoplasia Ⅱ( CINⅡ) associated high risk HPV with LEEP combined interferon and recombinant human interleukin -2.Methods One hundred and sixty patients with CIN Ⅱwere selected during Feb.2012 and Feb.2013.The patients were random-ly divided into four groups(40 patients in each group):the control group(LEEP),group A(LEEP united with in-terferon),group B(LEEP united with recombinant human interleukin -2)and group C(LEEP united with inter-feron and recombinant human interleukin -2).TCT,colposcope examination and HPV were reviewed ,and the curative effects , postoperative complications and negative conversion rate of HPV -DNA were compared after three and six months of the treatment .Results The cure rate and negative conversion rate of HPV -DNA in group C were 92.5%and 95%,which were significantly higher than others groups ( P<0.05) after six months of treatment .The time of wound healing ,duration of vaginal discharge and bleeding time in group C were also shorter than others groups(P<0.05).Conclusion The therapeutic effect of LEEP combined with interferon and recom-binant human interleukin -2 is superior to LEEP and LEEP united interferon or interleukin -2,which is not only shorter healing time ,but also fewer complications .The therapeutic method is worthy to be applied widely .

OBJECTIVETo summarize the clinical experience for the reconstruction of upper lip defects in different degrees.

METHODSDifferent treatment methods were selected to reconstruct the upper lip defects according to the subunit, size or location of defects on the upper lip. The therapeutic results were analyzed and compared.

RESULTSFrom Jan. 1998 to Apr. 2012, 243 cases with upper lip defects were treated, including 85 cases of traumatic defects, 110 cases of defects secondary to lip cleft and 48 cases of defects after tumor resection. 48 cases were treated with direct closure, 69 cases with cross-lip flaps, 5 Cases with orbicularis oris muscle flaps, 53 cases with unilateral labial flaps, 42 cases with bilateral labial flaps, 22 cases with lower lip flaps, and 1 case with forehead flap, 1 case with forearm flap and 2 with nasolabial fold flaps. The patients were followed up for 3-18 months with satisfactory results.

CONCLUSIONSSatisfactory results could be achieved for upper lip defects with appropriate treatment methods according to the defects degree and characters.

Cleft Lip ; surgery ; Cosmetic Techniques ; Dermatologic Surgical Procedures ; methods ; Humans ; Lip ; injuries ; surgery ; Mouth Mucosa ; Surgical Flaps ; Treatment Outcome

Adipocytes ; pathology ; Angiomyoma ; pathology ; Humans ; Nasal Cavity ; Nose Neoplasms ; pathology

Objective To investigate the linear relationship between invasive blood pressure and noninvasive blood pressure,providing references for clinical blood pressure monitoring.Methods Totals of 32 ICU patients were chosen,and their invasive blood pressure and non-invasive blood pressure were monitored at the same time by self-reflection methods.The data were collected and compared,and linear correlation was analyzed.Results Totals of 98 pairs of valid date were collected.The invasive systolic and diastolic blood pressure were respectively (146.93 ± 21.426) and (71.32 ± 13.152) mm Hg,higher than the non-invasive systolic and diastolic blood pressure which were (124.02 ± 19.417) and (68.86 ± 15.251) mm Hg,and the differences were statistically significant (t =15.301,3.363,respectively; P ＜0.05).And there was a linear relation between invasive blood pressure and non-invasive blood pressure (r =0.880,P ＜ 0.05).Conclusions There is statistically significant difference between invasive blood pressure and non-invasive blood pressure,which are not substitutable for each other.And non-invasive blood pressure monitoring values can be used to deduce and calculate the invasive blood pressure monitoring values.

Objective To find out the demands of critical patients' families about assurance scale and the cognition of the nurses to families' need in Emergency. Methods By Critical Care Family Needs Inventory (CCFNI), 100 family members of patients in Emergency Resuscitation Room / Emergency Intensive Care Unit (EICU) and 100 emergency nurses were surveyed. Results Among the 7 indexes, the differences of 4 indexes had no obviously statistical meaning in the family members' needs for AS and the nurses' congnition for AS (P>0.05). The 3 indexes, e. g. , truly answering the family members' questions, knowing the situation of disease change and feeling the hopeful improvement of disease, had significantly statistical meanings (P< 0.05). Conclusions Emergency nurses can meet the majority need of the families of critical patients, but there still exist some questions that need to be improved and advanced.

Objective To learn and analyze the patients' circulation of blood fluctuating rules in 24 hours after receiving the liver transplantation, conclude the policies of the circulation of blood monitor and nursing, and offer the basement for the clinical nursing program of liver transplanting. Methods Using the multifunctional electrocardiograph to observe the hver-transplanted ICU patients' heart rate , blood pressure and center venous pressure in the time points below: before operation, turning back ICU immediately and 2、6、12、 24、20、24 hours, and make the statistical analysis. Results The patients' heart rate ascend greatly after entering ICU 24 hours( P < 0. 01 ). When turning back ICU, their blood pressure had no greatly different (P > 0. 05). After 2 hours,the blood pressure become to distinct greatly until 24 hours. Center venous pressure had no statistical difference( P >0.05). Conclusions The liver-transplanted patients' circulation of blood changes greatly after operation. To avoid damage, we should strengthen the temperature monitor, and standardize the patient nursing management.

OBJECTIVETo study clinical characteristics of children with enthesitis related arthritis (ERA).

METHODSTwelve patients fulfilling the international league of associations for rheumatology (ILAR) criteria for classification of juvenile idiopathic arthritis (JIA) and ERA were referred to our department between August and November, 2002. Their gender, age, family history, clinical manifestations, imaging data, laboratory data and treatment regimens were analyzed retrospectively.

RESULTSOf the 12 patients, 11 were male, only one was female; their age ranged from 4 to 16 years, and the median age was 10.5 years. Ten (83%) of the patients were older than eight years. Among their first degree relatives, one had definite ankylosing spondylitis (AS), and 3 presented with a history of inflammatory low back pain. Enthesitis occurred in 9. Synovitis occurred in 11, most of which were oligoarthritis, predominantly affecting large joints of the lower limbs in an asymmetric pattern. Seven patients underwent CT scan, and only one had erosions of the sacroiliac joints to achieve a diagnosis of juvenile AS. Ten had fever at the onset, and one had a history of diarrhea preceding the symptoms of arthritis. Urethritis and balanitis circinata occurred in 3 cases fulfilling the classification criteria of Reiter's syndrome, with conjunctivitis in 2 and corneitis in one. Elevated inflammatory indicators such as white blood cell, neutrophil, platelet, erythrocyte sedimentation rate, C-reactive protein, immunoglobulins and serum complement C3 were common during the acute illness. Mild anemia was found in 4, and reactive hemophagocytosis in 4 bone marrow specimens. DNA of human parvovirus B19 was detected in sera of 2 cases. One had positive IgM antibody to coxsackie virus B. All 12 cases were rheumatoid factor negative and HLA-B27 positive. Nonsteroidal anti-inflammatory drugs and sulfasalazine were the mainstay of treatment. Corticosteroids were added in 3 cases as a "bridge" therapy due to the severe systemic inflammation. Methotrexate was used in 4 cases with refractory disease or with the hip involved, and in 2 of them cyclophosphamide was added.

CONCLUSIONThe clinical characteristics of ERA can facilitate an early diagnosis so as to avoid joint damage and disabilities.

Adolescent ; Anti-Inflammatory Agents, Non-Steroidal ; therapeutic use ; Arthritis ; drug therapy ; etiology ; Child ; Child, Preschool ; Female ; Humans ; Joint Diseases ; complications ; Joints ; pathology ; Male ; Retrospective Studies

OBJECTIVETo study the expression of Runx2/Cbfa1 in the developing dentin and differentiating odontoblasts.

METHODSA postnatal mice teeth developing model was built histologically. Immunohistochemical technique was adopted to determine the expression of Runx2/Cbfa1 in the developing pulpo-dentinal complex in mice.

RESULTSRunx2/Cbfa1 was merely present in predentin in the exact and before the 11th day's postnatal stages. Meanwhile, it was positively located in odontoblasts and dental pulp cells in root region, but negatively in coral part after the 11th day's stages.

CONCLUSIONRunx2/Cbfa1 may play an important role in the deposing of tooth dentin and in the differentiating of odontoblasts and pulp cells.

Animals ; Cell Differentiation ; Core Binding Factor Alpha 1 Subunit ; Dental Pulp ; Dentin ; Mice ; Odontoblasts ; Tooth

Objective TALE-TFs were adopted to provide a new way in detection of the expression result ofβ-ca-sein gene promoter-interesting gene expression cassettes in mouse fibroblasts.Methods TALE-TFs of eukaryotic expres-sion plasmid and expression cassette withβ-casein gene promoter and red fluorescent protein reporter gene were co-nucleo-fected into mouse fibroblasts by Amaxa nucleofector.Results and Conclusion β-casein gene promoter was activated by artificial TALE-TFs in the mouse fibroblasts.The way is a new expression verification system instead of mammary epithelial cells with fibroblasts.