Objective To verify an ELISA method for quantitative detection of antibody IgG against Shigella flexneri 2a and Shigella sonnei O-specific polysaccharide in human serum and use it to detect human serum samples before and after immunization with flexneri and sonnei dysentery bivalent vaccine.Methods The reference serum and quality control serum were mixed in a certain proportion to prepare 10 serum samples of different concentrations(numbered as S1～S10),which was determined continuously for 6 d by the indirect ELISA quantitative detection method provided by the enterprise,and the recovery rate and coefficient of variation(CV) were calculated.The reference serum content with a recovery rate of CV less than 20% was used as the limit of quantitation(LOQ).The serum sample S10 and reference serum were measured by the same method for 6 d,3 times of repeated test a day,and the CV was calculated;The inhibition rate of serum to polysaccharide was calculated by using the competitive inhibition ELISA with reference serum.The reference serum was detected by the method provided by the enterprise,the linear range was determined,the curve equation was obtained,and the correlation coefficient(R~2) was calculated.The verified method was used to detect 1 842 human serum samples before and after immunization with flexneri and sonnei dysentery bivalent vaccine.Results The recovery rates of Shigella flexneri 2a and Shigella sonnei polysaccharide antibody IgG in 10 serum samples were 73.21%～222.68% and 83.67%～123.56%,respectively;CV of precision verification between and within tests were all less than 30%;The inhibition rates of reference serum on 100 μg/mL Shigella flexneri 2a and Shigella sonnei polysaccharide were 85.95% and 88.42%,respectively;The linear ranges of Shigella flexneri 2a and Shigella sonnei polysaccharide antibody IgG were 0.05～0.125 and 0.025～0.125 EU/mL,with the R~2 not less than 0.99,and the LOQ were 0.050 and 0.025 EU/mL,respectively.The content of antibody IgG in serum after immunization with flexneri and sonnei dysentery bivalent vaccine was significantly higher than that before immunization.Conclusion The ELISA quantitative detection method provided by the enterprise had good accuracy,precision and specificity,and might be used for the detection of clinical serum of dysentery vaccine.

Malnutrition is highly prevalent in hospitalized children and has been associated with rele-vant clinical outcomes.Timely nutrition risk screening and reasonable nutrition support therapy subsequently for the high risk patients is the best and standard way.Five nutrition risk screening tools have been developed to identify the nutritional risk in hospitalized children.Even though nutrition risk screening is a necessary part of standard pediatric care,there are no international standardized screening tools for pediatric inpatients so far.The near future will provide us with comparative data on teh exi sting tools which may oc ntribute to deve-loping a standard for admitted nutrti ion risk screening in pediatrics.

Objective To explore the clinical value of Holter heart rate turbulence in diagnosis of patients at high risk for coronary heart disease.Methods 110 patients with suspected coronary artery disease,according to the results of coronary angiography (CAG),were divided into CAG positive group (67 cases) and CAG negative group (43 cases).Heart rate turbulence and heart rate variability were analyzed in two groups.The atrial fibrillation,atrial flutter,and pseudo-differential interference were removed,the qualified ventricular contraction was selected.Two parameters of HRT,the initial shock value (TO) and turbulence slope value (TS) were calculated.Results The average value of TO and TS was (0.36 ± 0.15) ％,(1.98 ± 0.52) ms/RR in positive group,those in the negative group were (-0.87 ± 0.36) ％ and (4.83 ± 0.65) ms/RR,there were statistically significant differences between two groups (t =1.953,1.976,all P ＜ 0.05).After 1 year follow-up,9 cases (13.44％) died in positive group,2 cases (4.65％) died in negative group,the difference was statistically significant (x2 =5.476,P ＜ 0.05).Conclusion The heart rate turbulence with dynamic cardiogram can help to early diagnose the patients at high risk of coronary heart disease.It was important to improve the survival rate of patients.The method is simple,inexpensive,effective,which is worth promoting in the clinic.

The research to improve outcomes in critically ill patients through nutrition support has steadily progressed over the past 4 decades. One current approach to this problem is the addition of specific nutrients as primary therapy to improve host defenses and improve the outcomes of critically ill patients. The field is referred to as“pharmaconutrition”,focusing investigations on each nutrient to understand its pharma-cological effects on immune and clinical outcomes. The purpose of this review was to introduce some of the known pharmaconutrients such as glutamine,arginine,ω-3 fatty acids,vitamin C,zinc,and selenium,regard-ing critical ill adults and children.

Objective To explore the efficacy and safety of hormone replacement therapy with combination of Fosamax and simple use of Fosamax in osteoporosis and steopenia of perimenopausal women.Methods One hundred and thirteen perimenopausal women with osteoporosis accepted treatment in Outpatient Clinic of Panyu Convalescent Hospital of Guangzhou from December 2010 to June 2014 were chosen and divided into the control group (n=55) and the observation group (n=58) according to therapeutic method.The control group was treated with Fosamax alone while the observation group was treated with HRT basing on the simple use of Fosamax.The therapeutic effect,lumbar and hip bone mineral density(BMD) level,tibial ultrasound velocity (SOS) and T values of tibia,gonadal hormone concentrations,endometrial thickness and adverse reaction of the two groups before and after the therapy were compared.Results (1) The total effective rate of the observation group after the treatment was 93.10％,significantly higher than tbat of the control group (78.18％,x2=3.829,P=0.034).(2) Before and after treatment in two groups,there were statistically significant differences in terms of lumbar spine BMD,hip BMD,SOS of tibia,blood follicle stimulating prime (FSH) and estradiol(E2) (P＜0.05),and there were statistically significant differences between the two groups after the treatment in terms of lumbar spine BMD,hip B MD,SOS of tibia,FSH and E2 (t =3.389,5.012,3.997,5.829,3.902;P=0.035,0.028,0.033,0.024,0.038).(3) There was statistically significant differences in term of the incidence of adverse reactions between the observation group and the control group (6.90％ vs.16.36％;x2=8.273,P=0.012).Conclusion Compared with alone use of Fosamax,the therapeutic effect of hormone replacement therapy with combination of Fosamax and in osteoporosis and steopenia of perimenopausal women is more significant with low occurrence rate of adverse reaction.It is worth popularization.

With advances on drug genomics of leukemia,the drug toxicity has been found to be associated with the drug metabolism gene polymorphisms in children with acute lymphoblastic leukemia.Current researches are to focuse on how to find a genotype to predict methotrexate toxicity effectively and treat patients individually.This review illustrates the association between three drug-metabolizing enzyme gene polymorphisms including methylenetetrahydrofolate reductase,reduced folate carrer and glutathione S-transferase and toxicity of methotrexate.

Objective:To research eyes refraction varieties and related influence factors undergone PKP.Methods:Sixty-two corneal diseases with PKP were followed up by ophthalmologist concentrating on corneal diseases on month 1,3,6,12,24,36.They had engaged in optometry and tried lens on patients besides regular examination,and had done statistical analysis of refraction change.Results:Eye refraction varieties were remarkable in 12 months especially in 1 to 6 months but stable after 12 months after PKP.Most of them had different degrees and kinds of astigmatism.Conclusion:Eyes refraction varieties were remarkable in 12 months after PKP and astigmatism occurred easily.There were some main factors:conditions before operation,design and manipulation during operation,and complications after surgery.

Objective To determine the content of rutin in Circanetten New tablets by HPLC. Methods The determination is performed at C18(150 mm? 4.6 mm, 5 ? m) column with temperature being 30 ℃ ; the mobile phase is consisted of methanol- water- glacial acetic acid(40 ∶ 60 ∶ 1.8) and UV detection wavelength is at 354 nm. Results The linear range was 6.008 to 48.064 ? g/mL( r=0.999 9) for Circanetten New tablets and the average recovery of rutin was 100.3% (RSD=0.5 % , n=5) .Conclusion The method is simple and effective, and can be used to control the quality of Circanetten New tablets.

Objective:To study the role of APACHEⅡscore pre-operation to infusion of blood preparation during the periods of hepatectomy.Methods:The correlation between APACHEⅡscore pre-operation and infusion of erythrocyte suspension and plasma were analyzed retrospectively in 104 cases who suffered from hepatectomy.Results:Those whose APACHEⅡscore were less than 5 had significantly lower volume of bleeding and lower infusion of erythrocyte suspension and plasma during the operation than those whose APACHEⅡscore were not less than 5(P