Objective To observe the efficacy and acute toxicity in non-myeloablative low-dose tatal body irradiation. Methods From January 2006 to January 2008, 27 cases of hematopoietic stem cell transplantation patients received non-myeloablative pretreatment program involving low-dose whole body irradiation. Pretreatment program: the total dose of TBI was 2 Gy, once completely. According to primary disease, physical condition, age, organ function and HAL coincide situation, the patients received different chemotherapy, including FUL, CTX, Ara-C, melphalan and so on. To prevent the graft-versus-host disease (GVHD), CSA/MMF was used. Results Hematopeietic reconstruction: the peripheral blood WBC in all 27 cases reduced to (0.05-0.9) × 109/L in 4 to 8 days, with the neutrophil count > 0.5 × 109/L in 8 to 22 days (median + 10.5 days), the platelet count > 30 × 109/L in 11 to 28 days (median + 14.5 days). The transplant rate was 85.2% (23/27). The fever rate was 65% and the infection rate was 37% (10/27). Hemorrhagic cystitis,hepatic veno-occlusive disease (HVOD), and other complications did not occurred. 4 cases developed aGVHD (15 %) and 5 cases developed cGVHD (19 %) out of 27 cases. Follow-up 3 to 22 months, 21 eases are still alive (77.8%). Conclusions Pretreatment with low-dose whole body irradiation of non-myeloablative hematopeietic stem cell transplantation proved to be simple and safe with fewer complications and broad indications. Program of TBI with total dose of 2 Gy (once completely) is a safe, less toxic and effective method. It could achieve the effectiveness of immune suppression.

Objective To study reasons for conversions to open surgery in gynecological laparoscopic operations.Methods Clinical records of 53 cases of conversions to open surgery out of 1 066 cases of gynecological laparoscopic operations from January 2000 to March 2005 in this hospital were analyzed retrospectively.Results The reasons for conversions included pelvic adhesion in 23 cases,difficulty in myomectomy in 10 cases,malignant tumors in 9 cases,and intraoperative complications in 3 cases,accounting for 43.4%(23/53),18.9%(10/53),17.0%(9/53),and 5.7%(3/53) of the 53 cases,respectively.Conclusions Pelvic adhesion,which leads to difficulties in laparoscopic performance,is the primary reason for conversion to open surgery.Proper patient selection and improved capability for distinguishing benign ovarian tumors from malignant ones can decrease the incidence of conversions.

AIM: To study the mechanism for biological effect of ? 1-adrenoceptors by inves tigating the molecular mechanism involving in the apoptotic processi on in myocardium. METHODS: The expressions of Bcl-2, Bax, cytochrome C, caspase-3 were determined by means of immunohistochemistry and molecular technique. RESULTS: Bcl-2 was increased but apoptosis, Bax, cytochrome C, caspase-3 were decreased when phenylephrine was used before ischemia. CONCLUSION: ? 1 -adrenoceptors may influence the expression of Bcl-2 ,change the permeabil ity of mitochondrion and adjust the expression of apoptotic protein to control apoptosis.

Carcinoma ; drug therapy ; genetics ; metabolism ; pathology ; radiotherapy ; Desmoplastic Small Round Cell Tumor ; metabolism ; pathology ; Diagnosis, Differential ; Gene Rearrangement ; Head and Neck Neoplasms ; drug therapy ; genetics ; metabolism ; radiotherapy ; Humans ; Keratin-20 ; metabolism ; Keratin-7 ; metabolism ; Lymphatic Metastasis ; Male ; Mediastinal Neoplasms ; drug therapy ; genetics ; metabolism ; radiotherapy ; Melanoma ; metabolism ; pathology ; Neuroectodermal Tumors, Primitive ; metabolism ; pathology ; Nuclear Proteins ; genetics ; metabolism ; Oncogene Proteins ; genetics ; metabolism ; Oncogene Proteins, Fusion ; genetics ; metabolism ; Rhabdomyosarcoma ; metabolism ; pathology ; Thymus Neoplasms ; drug therapy ; genetics ; metabolism ; radiotherapy

Accidents, Occupational ; Acute Disease ; Aniline Compounds ; poisoning ; Humans ; Occupational Diseases

Gene Targeting ; Humans ; Hypoxia-Inducible Factor 1 ; Hypoxia-Inducible Factor 1, alpha Subunit ; pharmacology ; Neoplasm Invasiveness ; pathology ; physiopathology ; Neoplasm Metastasis ; physiopathology

Objective: To determine if naloxone has reverse effect on cerebral ischemic injury following cardiac arrest. Method:Ten dogs underwent 15-min cardiac arrest and then were divided into two groups during 3-hour cardiopulmonary resuscitation (CPR). Group A(n=5): the animals were administered with conventional CPR;group B(n=5): in addition,at onset of CPR,an I. V. bolus of 0.2mg?kg~(-1) of naloxone,followed by infusion of 0.02mg?kg~(-1)?h~(-1). Result: Cerebral blood flow, Cerebral perfusion pressure in group B were significantly higher than those in group A (P

Child ; China ; Congresses as Topic ; Humans ; Pediatrics ; Syncope ; diagnosis ; etiology ; therapy

Objective: To explore the influence of Janus kinase inhibitor AG490 on phosphorylation of extracellular signal-regulated protein kinase (ERK) in the human breast cancer cell line MDA-MB-231, so as to discuss the effect of JAK on ERK signal transduction pathway and on invasion/ metastasis of breast cancer cells. Methods: MDA-MB-231 cells were treated with Janus kinase inhibitor AG490; MTT assay was used to examine the proliferation of MDA-MB-231 cells and the adhesion of MDA-MB-231 cells to artificial basement membrane matrigel after AG490 treatment. Invasion and metastasis of MDA-MB-231 cells were evaluated with transwell chamber after treated with AG490. The expression of P-ERK and MMP-9 protein was determined by Western blotting assay. Results: AG490 inhibited the proliferation of MDA-MB-231 cells in a time- and dose-dependent manner; AG490 also depressed the adhesion, invasion and metastasis of MDA-MB-231 cells. Meanwhile, the expression of P-ERK and MMP-9 protein was decreased after treatment with AG490. Conclusion: Our study indicates that JAK kinase can affect the activity of ERK signal transduction pathway through the phosphorylation of ERK. The inhibitory effects of JAK kinase on MMP-9 expression and invasion of breast cancer cells are associated with the blocking of the ERK signaling pathway.