Objective To observe the changes of coagulase activity and the DNA expression of coagulase of S.aureus during inducing vancomycin-resistant S.aureus in vitro.Methods Coagulase activity and DNA expression of coagulase of S.aureus were detected.Results The coagulase activity in 6 strains and the expression of coagulase DNA in 1 strain reduced.Conclusion Decreased coagulase activity and DNA expression are probably linked to vancomycin resistance of S.aureus.

Objective To induce vancomycin-resistantant S.aureus in vitro and to observe the changes of coagulase gene sequences of S.aureus and its relationship with vancomycin resistance.Methods DNA sequences of the coagulase were examined by polymerase chain reaction-sigle strand conformation polymorphism(PCR-SSCP) method and were identified by sequencing.Results The altered DNA sequence of coagulase in 1 strain of S.aureus was found.Conclusion The variation of DNA sequences of coagulase gene is probably linked to vancomycin-resistance in S.aureus.

Objective To investigate the prognostic factors in patients with sepsis and to provide the theoretical basis for clinical diagnosis and treatment. Methods A total of 141 septic patients were analyzed retrospectively. Patients were divided into two groups:those who were alive at time of ICU discharge ( survivors;n=83 ) and those who died or to give up before terminal stage ( nonsurvivors;n=58 ) . Then the clinical data were statistically ana-lyzed. Results In the death group: age, APACHE II score, blood urea nitrogen, serum creatinine, C-reactive protein were significantly higher than the survival group (P<0. 01) while albumin, pre albumin were lower than that in the survival group (P<0.01). The Logistic regression analysis illustrated: age ( > 60 years), APACHEIIscore ( >20 score) and blood urea nitrogen ( > 9 mmol/L) , serum creatinine ( > 176 μmol/L) , C-reactive protein level were independent predictors of death in patients with sepsis ( P<0 . 05 ) . Then drew the receiver oper-ating characteristic curve ( ROC curve) and analyzed the respective risk factor for predicting prognosis value of sep-sis with the help of ROC curve. Conclusion Age, high APACHE II score in early sepsis, increased urea nitro-gen, elevated serum creatinine and increased C-reactive protein levels were regarded as prognostic value indicators for adverse clinical outcomes in patients with sepsis.

Objective To explore the effect of acupuncture combined with rehabilitation training on unilateral spatial neglect (USN).Methods One hundred stroke patients with USN were randomly divided into a treatment group (n=50) and a control group (n=50).Both groups were treated with routine rehabilitation training for 4 weeks,while the treatment group was additionally treated with acupuncture.Before and after the treatment,the degree of USN was evaluated in both groups along with the Fugl-Meyer assessment,the modified Barthel index and the incidence of shoulder pain.Results No significant differences were found between the 2 groups in any of the measurements before the treatment.After the treatment the incidence of shoulder pain in the treatment group was significantly lower than that in the control group.All the other measurements were significantly higher in the treatment group than in the control group.Conclusion Acupuncture combined with rehabilitation training can significantly improve the symptoms of unilateral spatial neglect,improve motor function in a hemiplegic limb and improve performance in the activities of daily living.

Objective To explore the diagnosis value of expression level of peripheral blood microRNA-21 in early gastric cancer patients. Methods In 52 patients with Tis gastric cancer and 40 healthy subjects, expression levels of microRNA-21 in peripheral blood were quantitatively detected by real-time quantitative PCR(qRT-PCR),and diagnostic value of expression level of microRNA-21 in peripheral blood were evaluated by receiver operator characteristic (ROC) curve. Results Relative quantity expression level of microRNA-21 in peripheral blood in gastric cancer group and healthy subjects group were 2.286±0.592 and 1.318±0.357, and there was statistical significance (U=136.500, P=0.000). ROC curve analysis showed that ROC curve area of expression level of microRNA-21 in peripheral blood in diagnosing gastric cancer was 0.934(95%CI 0.887-0.982, P=0.000), and the best diagnostic boundary point value was 1.912. The sensitivity of expression level of microRNA-21 in peripheral blood in diagnosing gastric cancer was 86.5%, and the specificity was 95.0%. Conclusion Expression level of microRNA-21 in peripheral blood has important diagnostic value in early gastric cancer.

Objective:To evaluate the biological safety of commercial pure Ti (cpTi),Ti alloy Ti6Al4V and stainless steel 316L.Methods:According to the GB/T16886.5-2003 and YY/T0127.2-2009 standards,the extraction of the 3 materials was respectively prepared,cck-8 assay was used to test their cytotoxicity,single cell gel electrophoresis was used to test their genotoxicity,and in vivo test was used to test their acute systemic toxicity.Results:The cytotoxicity of 100％ extractions of 316L,Ti6Al4V and cpTi was graded to 2,1 and 0,the olive tal moment(OTM) 10.79 ±0.39,9.84 ± 1.78 and 0.92 ± 1.43,the percentage of tail DNA (1.22 ±0.06) ％,(0.92 ± 0.43) ％ and (0.43 ± 0.01) ％ respectively.No systemic toxicity and no body weight change was observed in the acute systemic toxicity test in mice.Conclusion:cpTi has better biologic safety than Ti6Al4V and 316L.

Objective To investigate the usage of antibiotics in pediatrics patients in Changchun,and detect the bacterial drug resistance of main pathogens for direction of the appropriate clinical choice of drug antibiotics.Methods A total of 900 cases of infectious patients were retrospectively investigated in antibiotic usage in the ⅢA hospital in Changchun from January 2003 to November 2005 and the data were statistics analyzed.The bacterium were isolated from all kinds of specimen of children in hospital patients from December 2004 to November 2005,according to the method of "Clinical Medicine Test Handbook".All bacterial isolated from bacterial susceptibility tes-(ting) was done with Kirby-Bauey method.Tests were performed according to the guidelines of National Committee for Clinical Laboratory Standards(NCCLS)(2004).Results Antibiotics were administered in all of the cases.Four categories and 11 kinds of antibiotics were used.Usage of the first six antibiotics were as followed: ceftezole(22.3%),cefoperazone-sulbactam(21.6%),amoxicillin clavulanic acid((19.6%),)penicilin(17.2%),azithromycin(16.40%),oxacillin(10.1%).The rate of antibiotic use was 60.6% for solo use,70.7% for bigeminy use,81.7% for intravenously guttae,18.3% for peroral administration.Eight hundred and eighteen strains of pathogens were isolated.Gram-positive coccus,gram-negative accounted for 48.0% and 52.0%,respectively.All bacterium isolated showed a high resistance against penicillins and cephalosporins.Drug resistance rate of Gram-positive bacteria to penicillin was more than 50.0%,CNS,S.aureus,E.faecalis and S.pneumonic to erythromycin and cphalosporins was over 71.4% and 35.7%.The antibiotic remaining the most active against Gram-positive bacteria was vancomycin.The drug resistance rate against imipenem and ciprofloxacin were lower than(25.0%.)The antibiotic resistance rate of Gram-negative bacteria to ampicillin,cephalosporins and aztreonam were more than 83.0%,(40.0%) and 32.0%,to piperacillin and amoxicillin-clavulanic acid were morn than 68.9%,except P.aeruginosa(33.3%).The susceptibility of Gram-negative bacteria was imipenem(

Objective:To explore the relation of fear of success to self-esteem,academic self-handicapping, and defensive pessimism in college students.Methods:Totally 291 college students were surveyed with the Self-Es-teem Scale (SES ),Academic Self-handicapping Questionnaire (ASHQ ),Defensive Pessimism Questionnaire (DPQ)and Fear of Success Questionnaire for Undergraduates (FOSQ).The structural equation modeling (SEM) and mediation analysis were used to test the hypotheses.Results:Self-esteem negatively predicted quality of life (β=-0.16),working pressure (β=-0.17)and interpersonal relationship (β=-0.14).In the 95% level of confi-dence,the lower range output of Bootstrapping in the path model of self-esteem through self-handicapping,defen-sive pessimism to a fear of success respectively was -0.29 and -0.14.The upper range output of those respec-tively was -0.14 and -0.01.Conclusion:Academic self-handicapping and defensive pessimism may play a medi-ating role in the relationship between self-esteem and fear of success.

Abdominal aortic aneurysm (AAA) is a common aortic disease causing death. Many factors are associated with AAA rupture, such as aneurysm diameter, shape, intraluminal thrombus, and hemodynamic changes. It has been shown that hemodynamic changes may be one of the main risk factors for AAA rupture. There are several imaging modalities to evaluate AAA, in which Doppler ultrasound and magnetic resonance blood flow imaging are capable of assessing the hemodynamic changes in AAA. This review will discuss the risk factors for rupture and imaging advancement of AAA.

ObjectiveTo observe the therapeutic effect of standing bed on orthostatic hypotension with cervical spinal cord injury. Methods36 patients with cervical spinal cord injury were divided randomly into the standing bed group and routine treatment group. The blood pressure of all patients were measured every day during rehabilitation treatment. The function of spinal cord was assessed before and after treatment.ResultsThe function of sensory and motor in the two groups were all improved, but there was no statistical difference between the two groups in the grades of spinal cord function. There were 27 patients who had orthostatic hypotension in the study. All the patients with complete spinal cord injury suffered the orthostatic hypotension. There was no improvement in blood pressure of complete spinal cord injury after treatment. In incomplete spinal cord injury patients, there was a significant improvement and the difference of blood presure between lying and standing was decreased in standing bed group after treatment (P<0.05). Compared with routine treatment group, the blood pressure in standing bed group was improved (P<0.05). Conclusions Cervical spinal cord injury could have orthostatic hypotension. Complete spinal cord injury would suffer orthostatic hypotension in different degree. The standing bed treatment could improve the orthostatic hypotension in incomplete spinal cord injury.