Objective To evaluate the sterilization effect of Er:Yag laser irradiation combined with 2%chlorhexidine gel on single tube premolar enterococcus faecalis infection model.Methods After disinfection test was performed without molars bacterial infection,200 single rooted premolar root canal were selected.10μL enterococcus faecalis( ATCC 29212) bacterium suspension were inoculated into single intratracheal with high pressure sterilization and and then were cultured for 2 weeks.200 infected premolar model were divided into four groups:control group (n=50),A group (n=50),B group (n=50) and C group (n=50).The control group did not receive any disinfec-tion treatment,group A received Er:Yag laser irradiation and 2%chlorhexidine gel disinfection,B group received only Er:Yag laser irradiation,the C group received only 2% chlorhexidine gel disinfection.After disinfection,premolars were put into 10mL sterile water,mixed thoroughly,and then 1mL liquid were taken and diluted by10 times.Then the 200μL dilutions lipuid were coated on BHI agar plate,and plate could be selected for counting colony count.Results The average number of colonies was(127 ±12) in the control group,average number of group A(10 ±3) and which was lower than that group C (48 ±6)(t=12.005,P=0.000) and group C (43 ±5) (t=19.277,P=0.000). There were only 2 cases,which had obvious ablation phenomena in group A.Conclusion Disinfection effect of Er:Yag laser irradiation combined with 2%chlorhexidine gel is better than that of Er:Yag laser irradiation or 2% chlo-rhexidine gel disinfection.The incidence rate of the ablation phenomenon was low.

Macrophages are important immunocytes in human body , playing an important role in antigen and inflammation process.In recent years , it has shown that macrophage is a kind of cell mass with high plasticity , heterogeneity , diverse and variable immune functions .They can be polarized into different phenotypes in different microenvironments or stimulations , playing different roles in the process of inflammation .There are a variety of factors which can modulate macrophage polarization process , such as the de-velopment and progression of metabolic diseases .The polarization of adipose tissue macrophages and the factors in the regulation of macrophage polarization are summarized in this paper .

BACKGROUND: Deposition of lipofuscin (LF), decrease of superoxide dismutase (SOD) level and increase of malondialdehyde (MDA) level are important manifestations of senility. Some Chinese medicinal herbs have obvious advantage in delaying senility.OBJECTIVE: To explore the effects of fuling shengmai yin (FLSMY)serum on SOD, MDA and LF level of rat hippocampal neurons in vitro with vitamin E serum and blank serum as controls.DESIGN: Randomized controlled study.SETTING: Experimental Animal Department of Tongji Medical College,Huazhong Science and Technology University; Cell Culture Center of Anatomy Teaching and Research Section of Tongji Medical College,Huazhong Science and Technology University; Scientific Research Department of Wuhan Municipal Hospital of Traditional Chinese Medicine.MATERIALS: From October 2000 to February 2001, the experiment was conducted at the Experimental Animal Department of Tongji Medical College as well as Cell Culture Center of Anatomy Teaching and Research Section of Tongji Medical College, Huazhong Science and Technology University. Six Japanese male white rabbits with big ears, fed normally and weighting (2.5±0.1)kg, were divided into three groups with 2 in each group.Thirty newly born SD rats aged 1-2 days, of clean grade and either gender,were selected.METHODS: [1] Hippocampal neurons were inoculated to 24-well culture plates with the density of 2.5×105/L. Cells in each well were randomized into three groups: A. FLSMY group (fuling shengmaiyin composed of Poria,Radix Ginseng, Radix Ophiopogonis and Fructus Schisandrae; each milliliter contained 1.5 gram of the herbs); B. drug control group (serum containing vitamin E); C. blank control group (blank serum). Each group had 8 wells. After 24 hours, 1 mL serum containing FLSMY, 1ml serum containing vitamin E, or 1 mL blank serum was added into each plate well of corresponding groups. [2] On the 6th day of cell culture, XO-HPX reaction system was added to induce cell injury, in which senility model was established by producing hyperoxide anion free radicals. [3] In the process of cell culture different drug was added to the corresponding different group plate well during replacement of culture fluid each time. [4] The level of SOD, MDA, and LF was detected after 15 days of culture.MAIN OUTCOME MEASURES: Level of SOD, MDA and LF in rat hippocampal neurons.RESULTS: SOD level was higher in FLSMY group than in blank control group (P ＜ 0.01), whereas MDA level in FLSMY group was lower than that in vitamin E group (P ＜ 0.05); LF level was lower in FLSMY group than in blank group (P ＞ 0.05).CONCLUSION: FLSMY as a drink is composed of traditional Chinese medicinal herbs: Poria, Radix Ginseng, Radix Ophiopogonis and Fructus Schisandrae; each has the properties of anti-oxidation and eliminating free radicals. FLSMY can strengthen SOD activity, lower MDA level, and inhibit LF formation. Therefore, FLSMY can delay senility through protecting neurons.

Objective To observe the effect of the dental operative microscope(DOM) and the ultrasonic apparatus in detecting the mesiobuccal root canal orifice of the maxillary first permanent molar(MFPM). Methods According to traditional method,the pulp chamber was opened and the second mesiobuccal root canals(MB2) were explored in 76 MFPM teeth.If MB2 was not found,then we modified the shapes of the pulp chambers and detected the orifices.At last,MB2,not found under naked eyes,was explored with DOM and ultrasonic technique. Results The detection rate of the second mesiobuccal orifice of MFPM was 81.6%.The rate was only 31.6% when using traditional method,while after modifying the shapes of the pulp chambers there was an increase of 30.2% under naked eyes and then another 19.7% increase was obtained by using DOM and ultrasonic apparatus.The distance between two mesiobuccal root canal orifices was(1.42?0.51) mm. Conclusion It is helpful to find some invisible orifice of MB2 by using DOM and ultrasonic technique,and it would be beneficial to improve the root canal therapy.

Aim To search for small molecule NGF mimetic s using a primary screening model.Methods Both rapid quantitati ve bioassay for NGF(-like) activity by measurement of neurite extension in NGF -induced PC12 cells and a following MTT technique to monitor cells alive were u sed.Results More than 1 600 compounds were tested using the se methods and several effective samples with lower cytotoxicity were found. One of them, the compound B,was in good dose-and time-effect relationship. Conclusion All above studies provide a useful model and chemical info rmation for further screen of neurotrophic mimetics and neural cell tumor induce rs.

Adolescent ; Cardiology ; methods ; trends ; Child ; Child, Preschool ; China ; Cost-Benefit Analysis ; methods ; Diagnostic Tests, Routine ; economics ; Female ; Humans ; Male ; Pediatrics ; methods ; trends ; Syncope, Vasovagal ; diagnosis

[ ABSTRACT] AIM:To compare the expression of SIRT2 in ovarian surface epithelial ( OSE) cell line and serous ovarian carcinoma ( SOC) cell lines, and to investigate the effects of SIRT2 on the cell proliferation, migration and inva-sion.METHODS:The expression levels of SIRT2 in the OSE cell line and the SOC cell lines were determined by Western blot.The SIRT2 siRNAs and overexpression construct were designed and verified.Transient transfection of SIRT2 siRNAs or overexpression construct was performed, and the effect of SIRT2 on the cell proliferation, migration and invasion was e-valuated.RESULTS:SIRT2 levels in the 5 strains of SOC cell lines were significantly lower than that in the OSE cell line.SIRT2 knockdown in HOSEpiC cells significantly enhanced the ability of cell colony formation and accelerated the cell growth rate.On the contrary, overexpression of SIRT2 in HO8910 cells dramatically repressed the number of cell colonies and cell activity.SIRT2 significantly changed the ability of ovarian cell migration.Knockdown of SIRT2 facilitated the cell invasion.CONCLUSION:The expression of SIRT2 in the SOC cells is significantly down-regulated.In the OSE cells, SIRT2 acts as a tumor suppressor and mediates the inhibition of cell proliferation, migration and invasion.

The ongoing health reform features direct hosting of county-level hospitals by provincial level hospitals as an important means and effective attempt to improve comprehensive service capacity of the former.This paper introduces the“Hanchuan Model”,in which Hubei People’s Hospital has hosted Hanchuan city People’s Hospital,and analyzed the operation performance and hurdles found.Based on such studies,the authors recommend on the vertical integration of medical resources by means of hosting, and comprehensive reforms of county public hospitals.

Objective To investigate the value of acoustic radiation force impulse (ARFI) on evaluating the renal tubular atrophy and interstitial fibrosis of IgA nephropathy(IgAN).Methods 117 patients diagnosed with IgAN,and 80 normal cases were examined with conventional ultrasonography and ARFI,recording the shear wave velocities(SWV),the hemodynamics and the renal biopsy results to quantify the elastic changes of renal cortex,with the aim of assessing AFRI's advantages on early diagnosing the impair of kidney.Results The SWV value of renal cortex,renal medulla of IgAN group were smaller than that of normal group (P ＜0.05),while the collective system SWV value had no difference(P ＞0.05).According to the ROC curve,the cut-off value of SWV in renal cortex was 2.7 cm/s.The accuracy of the diagnosis was 71 ％,which was better than RI (61.9％).The SWV value of the renal cortex decreased with the different stage of 1,2,3,4,but stage 5 was larger than stage 4.The degree of renal tubular atrophy and interstitial fibrosis(T) can be classified by the SWV of renal cortex,and the SWV of T2 was larger than T1 (P ＜0.05).Conclusions By quantifying the elastic change of kidney,ARFI technology is able to distinguish the degree of renal tubular atrophy and interstitial fibrosis,diagnose renal impairment early and judge the clinical stages.Furthermore the sensitivity,specificity,accuracy of it are superior to hemodynamic.

Objective To evaluate the clinical effect of local POME optical treatmeat in patients with severe vulvovaginal candidiasis (VVC) . Methods Ninety-eight patients, who were diagnosed as severe vulvovaginal candidiasis from January to December 2009 in Beijing Wuzhou Women's Hospital gynecologicalclinic,were enrolled into this study and divided into control and physiotherapy groups randomly. The patients of control group (re =48) were treated with oral Fluconazole and had Miconazde Nitrate suppositories settlement into vagina, while in the physiotherapy group (n =50) the patients accepted local POME optical treatment combined with the same medications as control. The therapeutic effect were evaluated and compared according to the subjective symptoms and VVC scores in both groups. Results The clinical symptoms of physiotherapy group were relieved within 4 hours after the treatment, which were significantly depressed compared to before the treatment (itching score 1.4 ±0.3 vs. 2. 8 ±0. 5,P <0. 05;pain score 1.5 ±0.4 vs. 2. 8 ±0. 3,P <0. 05),while obvious improvements were observed in control group untill 48 hours later (itching score 1. 8 ± 0. 6 vs.2.9 ±0.3,P<0. 05;pain score 1.5 ±0.5 vs. 2. 8 ±0.4,P <0. 05). The VVC scores were significantly lower in physiotherapy group than control after treatment at different timepoints (24 h VVC Score 5. 3 ± 1. 6 vs. 9. 5 ±2. 1,P<0. 05;48 h Score 4. 8 ±1.0 vs. 7.9 ±2. 3,P<0. 05),but no significant difference was observed in the comparison of VVC Scores between two groups 72 h after treatment (P > 0.05). Conclusion Local POME optical treatment combined with drug treatment in severe VVC patients not only has better effect than drug treatment alone,but also relieves clinical symptoms more quickly.