Medical imaging is a medical discipline with strong practicality.Picture archiving and transmission system ( PACS ) provides a convenient and effective method for the clinical teaching of diagnostic imaging,especially for the students from the University of Traditional Chinese Medicine.Applying PACS is more convenient to gain and manage typical images,more effective to study and teach individually and easier to probe novel method for medical imaging.

Objective To explore the diagnosis value of expression level of peripheral blood microRNA-21 in early gastric cancer patients. Methods In 52 patients with Tis gastric cancer and 40 healthy subjects, expression levels of microRNA-21 in peripheral blood were quantitatively detected by real-time quantitative PCR(qRT-PCR),and diagnostic value of expression level of microRNA-21 in peripheral blood were evaluated by receiver operator characteristic (ROC) curve. Results Relative quantity expression level of microRNA-21 in peripheral blood in gastric cancer group and healthy subjects group were 2.286±0.592 and 1.318±0.357, and there was statistical significance (U=136.500, P=0.000). ROC curve analysis showed that ROC curve area of expression level of microRNA-21 in peripheral blood in diagnosing gastric cancer was 0.934(95%CI 0.887-0.982, P=0.000), and the best diagnostic boundary point value was 1.912. The sensitivity of expression level of microRNA-21 in peripheral blood in diagnosing gastric cancer was 86.5%, and the specificity was 95.0%. Conclusion Expression level of microRNA-21 in peripheral blood has important diagnostic value in early gastric cancer.

Asthma ; Child ; Humans

Objective To explore the improvement of teaching effects for clinical medical undergraduates by combining methods of lectures and simulations practice.Methods Totally 40 2007 grade five-year clinical medical undergraduates were randomly divided into five groups,eight undergraduates each group.During clinical practice of anesthesiology teaching in the fifth semester,mini-lecture combing theoretical lesson with simulations practice was conducted for 45 rain including preoperative preparation,spinal epidural anesthesia,general anesthesia,brachial plexus block and cervical plexus block.Results were evaluated by questionnaires.40 copies were handed out and the effective recovery rate was 100％.Results All students approved this kind of teaching activities; 97.5％ believed that it can arouse their interest in study; 95％ wrote down the lectures and operation process,all students listened carefully and operated meticulously.Conclusions Lectures combined with simulations practice promote students' operation capacity and thinking ability thus to raise the basic clinical skills of students.

Humans ; Ischemia ; metabolism ; pathology ; therapy ; Ischemic Postconditioning ; methods ; Myocardial Reperfusion Injury ; metabolism ; pathology ; therapy

Objective To analyze the clinical features of organic damages in Kawasaki disease(KD),and to improve the diagnosis and treatment level.Methods Two hundred and two cases with KD admitted in Children's Hospital Affiliated to Capital Institute of Pediatrics from Jan.2000 to Oct.2007 were reviewed,and their blood routine,urine,stool routine,liver function,kidney function,chest X-ray,and so on,were investigated.The incidence of organic damages in KD was evaluated.Results Among the 202 KD children,137 were male and 65 were female,the male-female ratio was 2.11.0.Most of them were infants,and 71.3% of them were younger than 3 years old.The damages of hematological system were detected in 78/202 cases(38.6%),respiratory system 76/202 cases(37.6%),cardiovascalar system 68/202 cases(33.7%),urinary system 52/202 cases(25.6%),digestive system 23/202 cases(11.4%),and central nervous system 3/202 cases(1.5%).On the other hand,3 cases complicated with septemia,1 case with juvenile rheumatoid arthritis and 1 case with Henoch-Schonlein purpura.Conclusions The data indicate that the incidence of hematological system involvement in the sick-children with KD were highest,with more than 2 organs involved simaltaneously.Many organic damages were involved in KD and diverse clinical manifestation could be found.Physicians should understand not only the typical features but also the rare symptoms and organic damage so as to prevent misdiagnosis and improper treatment.J Appl Clin Pediatr,2009,24(1):31-32

Objective To elucidate clinical and pathological features of obesity-associated focalsegmental glomerulosclerosis(FSGS). Methods Clinicopathological data of thirteen obese cases (BMI3≥28kg/m2) with FSGS(OB-FSGS) and thirteen non-obese cases(BMI

Objective To study clinical therapeutic effect of endovascular embolization on cerebral arteriovenous malformation(cAVM). Methods In digital subtraction angiography (DSA), 23 patients suffering from cAVM were treated by endovascular embolization with NBCA through microduct technique. 6 cases were embolizated only one time, and 17 cases more than one time. 11 patients were treated with gamma knife after embolization. Results Among 23 patients, 6 cases of cAVM were embolizated completely, 4 cases more than 95%, 9 cases 75% to 95%, and 4 cases less than 70%. 3 patients occurred dizziness and headache after embolization. All patients were followed up after treatment, 4 cases had seizure, and the others' symptoms were obviously improved. Conclusion Endovascular embolization was a safe and effective method for treating cAVM, and combination of embolization and gamma knife could improve the clinical efficiency.

Background Epithelial-mesenchymaltransition (EMT)isamajorcontributortothe pathogenesis of posterior capsular opacification(PCO).Kruppel-like factor 6 (KLF6) is a zinc finger protein,which can be stimulated by high glucose in proximal tubule cells and involved in transforming growth factor beta (TGF-β)induced EMT of diabetic nephropathy.ObjectiveThis study was designed to investigate the effect of high glucose on the expression of KLF6 and its target genes( TGFB1,TGFBR1,COLIA1,HSP47) in human lens epithelial cells (LECs).MethodsHuman LECs(SRA01/04) were cultured and exposed to different concentration of glucose.The expressions of KLF6 mRNA and protein were analyzed by real time polymerase chain reaction( real time PCR) and western blot after treatment with high glucose.The expressions of KLF6 target genes were analyzed by real time PCR to evaluate the EMT of SRA01/04 cells.ResultsCompared with the control group(5.5 mmol/L),the relative mRNA levels of t-KLF6 and wt-KLF6 in SRA01/04 treated with high glucose(22.2,44.4,66.6 mmol/L) increased obviously (F =72.53,42.02,P＜0.01 ).Then,the concentration of 22.2 mmol/L was used in the next experiments.The relative mRNA levels of t-KLF6 and wt-KLF6 increased to the peaks after treatment with high glucose for 12 h,and began to decrease after 24 h until lower levels after 48 h ( F =100.12,125.52,P ＜ 0.01 ).Western blot showed that the expression of KLF6 protein was also upregulated by high glucose treatment.With the promotion of the expression of KLF6 gene,the relative mRNA levels of TGFB1,TGFBR1,COLlAl and HSP47 of treated cells also respectively increased after treatment for 12 h,and began to decrease after 24 h until nearly at the levels of the control groups after 48 h( F=6.73,162.35,64.39,12.05,P＜0.05 ).ConclusionsIt was concluded that high glucose induced the expression of KLF6 in human LECs,and KLF6 transiently stimulated the expression of target genes TGFB1,TGFBRl,COLlAl and HSP47 which were mainly involved in the mechanism of EMT.

Objective:To observe the clinical effects of integrated disease and TCM syndrome on patients with second degree atrioventricular block.Methods:Because of the lentor cardiac rhythm characteristics of econd degree atrioventricular block,the herbs which had the effect in increasing cardiac rhythm were selected and were used in combination of clinical syndrome differentiation to treat 35 cases of second degree atrioventricular block.Results:The total effective rate was 88.6%.Conclusion:Integrated differentiation of disease and TCM syndrome had significant effects on second degree atrioventricular block.