Objective:To evaluate the antileukemic activity and side effects of topotecan in patients with refractory and relapsed acute myelogenous leukemia(AML).Method:12cases of refractory and relapsed AML were treated with topotecan combined with chemotherapy,8 accepted 1 course,and the others accepted 2 courses.Result:4 patients achieved complete remission.3 had partial remission.The total response rate was 58%.The main side effect was myelosuppression.Conclusion:The topotecan-based induction therapy has antileukemic effect in some patients with refractory and relapsed AML.

Objective　To investigate the direct antitumor effect of several surface molecules of Bifidobacterium bifidum 1101 such as whole peptidoglycan (WPG), lipoteichoic acid (LTA), and polysaccharides (PS) on human colonic adenocarcinoma cell line LoVo. Methods　MTT assay and flow cytometry (FCM) were used to evaluate the effect of Bifidobacterial surface molecules on the growth of LoVo cells. Morphological study, fluorescent polarimetry and differentiation-specific enzyme analysis were used to determine whether LTA treatment could induce the differentiation of the cells. Results　LTA but not WPG or PS significantly inhibited the proliferation of LoVo cells in a dose and time dependent manner. Cell cycle analysis by FCM revealed that LTA induced changes in cycle distribution resulting in G0/G1 phase commitment. Morphological alterations observed by light and electron microscopy indicated maturity of cytoplasm and nucleus following treatment by 50μg/ml of LTA. Membrane fluidity was also altered, and intestinal alkaline phosphatase specific for the differentiation of LoVo cells roused dramatically during culture in LTA. It was further found that LTA elevated ［Ca2+］i which resulted from Ca2+ influx instead of the release of stored calcium. Conclusion　These observations indicated that LTA was able to inhibit the growth of LoVo cells and induce the differentiation of the cells. Calcium influx may play an important role in this process.

Psoriasis is a chronic inflammatory disease related to genome-wide and surroundings, it is important to develop a suitable animal model to research psoriasis pathogenesis and evolve pharmacotherapeutics. With the development of transgenetic technology in the past few years, psoriasis virulence gene animal model become a hotspot. Research of animal model of human psoriasis genes is reviewed in the paper.

Objective To study the effect of femoral offset reconstruction ON the Harris hip score and the hip ROM (range of motion) after artificial hip replacement in femoral neck fractures. Methods Follow-ups were conducted for 47 patients who had undergone artificial hip replacements for their unilateral femoral neck fractures. We took X-ray pictures to compare the femoral offsets at the affected and unaffected hips. The operated hips were evaluated by the Harris hip score. Statistical analyses were performed with SPSS l2.0 software. Results The Harris hip score of the operated hip was positively related to femoral offset reconstruction(t=7.25,P＜0.001).There was a significant and positive correlation between femoral offset and the hip abductor ROM(r=0.80,P＜0.001).The rate of femoral offset reconstruction had no significant difference between total hip arthroplasty and bipolar hip arthroplasty (χ2=0,P＞0.05).Conclusions Reconstruction of femoral offset after artificial hip replacement in the femoral neck fractures can improve funetional recovery and ROM of the hip. Femoral offset reconstruction is uncorrelated to the selection of total hip arthroplasty or bipolar hip arthroplasty.

Objective To study the aplication effect of fiber bronchoscope examination in the treatment of respiratory internal medicine emergency and its effect on the quality of life of patients.Methods 156 patients with emergency were randomly divided into the observation group and control group,each group in 78 cases.The control group was given routine treatment plan according to the primary disease type which was the respiratory treatment of the corresponding diseases,the observation group in the control group was added fiberoptic bronchoscopy based on the treatment.Compared blood clinical curative effect,heart rate and quality of life score changes of the two groups of patients after treatment.Results The total effective rate of the observation group was 94.87%,which was significantly higher than 81.01% of the control group(χ2 =6.28,P <0.05).The pH,PaO2 and SaO2 in the observation group after treatment were(7.35 ±0.04),(73.23 ±9.11)mmHg and(92.33 ±4.32)%,which were significantly higher than the corresponding index in the control group(t =3.97,6.08,4.96,all P <0.05);while PaCO2 and HR were (51.53 ±9.24)mmHg,(91.69 ±12.42)times/min,which were significantly lower than the corresponding index in the control group(t =6.73,5.13,all P <0.05);The quality of life score in the observation group was(54.04 ± 10.94)points,which was significantly lower than(77.23 ±12.32)points of the control group(t =5.86,P <0.05). Conclusion Fiberoptic bronchoscopy for respiratory internal medicine emergency treatment can significantly improve the clinical curative effect of treating disease and patient quality of life.

Objective To evaluate the methods for improving diagnostic ratio of unruptured ectopic pregnancy with ultrasonography. Methods A total of 139 patients of small adnexal cysts with thick wall were predicted respectively with sonographic indexes. All patients (61 with unruptured ectopic pregnancy and 78 with small corpusluteum) were confirmed postoperatively or with long-term follow up, and the results were compared. Results The diagnostic sensitivity of with hyperecho or isoecho compared with endometrium, hyperecho compared with ovarian tissue, adnexal cyst without small follicle around was 86.89%, 93.44% and 96.72%, and the specificity of three predictors was 93.59%, 85.90% and 70.51%, respectively. Two sonographic indexes together had a sensitivity of 98.36% and a specificity of 94.87% for diagnosing ectopic pregnancy. Conclusion Observing the relation of adnexal cyst and ovarian and the echogenic of cyst wall are specific and sensitive for the diagnosis of ectopic pregnancy.

Objective To investigate and analyze the effects of problem-based learning used in clinical nursing education. Method The research papers on the effect of problem-based learning used in clinical nursing education were retrieved via CNKI, Wanfang,VIP,PubMed,ProQuest and Google scholar websites and then analyzed by Revman5.0 software.Results Totally 11 RCTs were included.Meta-analysis showed that PBL could significantly promote students’examination scores,self-study ability, critical thinking and clinical skill,but insignificantly improved the effect in improvement of their theory learning,interest and ability in cooperation.Conclusions PBL is a scientific clinical teaching method,which can stimulate students’initiative and exploratory learning and enhance effects of clinical teaching.Meanwhile,we suggest formulating evaluation criteria and implementing rigorous RCT designs in the future.

Chloridion participated in various kinds of biological function, such as cellular immunologic response , cell migration, cellular proliferation and differentiation, apoptosis. This kind of functional multiplicity corresponded with different gene encoding different chloride channel. It indicated that chloride channel function change or depletion concerned with many kinds of corneal disease. Therefore, the study of corneal chloride channel was very important This article summarized the effect of corneal chloride channel and its advances, and investigated the relationship between corneal disease and chloride channel.

Hardy-Weinberg equilibrium test is the base of genetic epidemiology. The new methods for Hardy-Weinberg equilibrium test involve: X chromosome-linked single nucleotide polymorphism Hardy-Weinberg test, inbreeding coefficient(F) test, an incomplete enumeration algorithm for an exact test of Hardy-Weinberg proportions with multiple alleles, and graphical tests for Hardy-Weinberg equilibrium based on the ternary plot. It is necessary to conduct Hardy-Weinberg equilibrium test in genetic epidemiology studies and adjust the associations as deviation of Hardy-Weinberg equilibrium occurs.

Objective To solve the problem about abundance information but poverty of knowledge by training the information analysis ability of users.Objects Medical graduate students who have the basic knowledge of literature retrieval.Curriculum Bibliometric parameters,reference management software,literature analysis software,Excel and the analysis function of bibliographic database were explained in 18 class hours.Results Satisfactory results have been got.Discussion Users can analyze retrieval results effectively after short time learning with no additional cost.