L-10 has been recognized as an irnmune suppressive cytokine which inhibits Ag-specific activation and proliferation of T cells. It also inhibits Ag presenting capacity of monocyte/macrophage and down-regulates monokine production. However it has also shown that IL-10 has stimulatory effect on immune effector cells in recent studies. This report shows that IL-10 has direct stimulatory effect on antitumor cytolytic activity suppressed by TGF-B. To assess the effect of IL-10 on cytolytic activity against tumor, spleen cells prepared from tumor-bearing mice were cultured with mitomycin C-treated MOPC-315 cells in the presence of IL-10. Unexpectedly, IL-10 was able to reverse the cytolytic activity suppressed with TGF-B. The stimulatory effect of IL-10 was dependent on the addition time of IL-10. At day 0, 4, those effects were shown higher than those of the other days. Also, the stimulatory effect of IL-10 showed specificity against MOPC-315 tumor cells. To elucidate the role of endogenous IL-10, TGF-B in MLTC cultures, anti-IL-10 and anti-TGF-B mAb were used. The inhibition of IL-10 release in MLTC cultures by using anti-IL-10 mAb resulted in the suppression of cytolytic activity against MOPC-315 tumor cells. Taken together, although IL- 10 has been recognized as a strong immunosuppressive cytokine derived of tumor cells, IL-10 showed the direct stimulatory effect on the antitumor cytolytic activity of spleen cells.
Animals ; Interleukin-10* ; Mice ; Mitomycin ; Sensitivity and Specificity ; Spleen* ; T-Lymphocytes ; Transforming Growth Factor beta

Objective To evaluate the effect of laparoscopic ultrasonography in laparoscopic cholecystectomy. Methods Laparoscopic ultrasonography system of Sharplan Company was used for the examination of the liver, gallbladder, bile duct and pancreas in 410 patients performed by laparoscopic cholecystectomy. Results The rates of biliary duct abnormality, preoperative diagnosis change, preoperative inexpectant disease finding, bile injury and residual stone were 3.5%, 4.4%, 2.4% and 0%, respectively. Conclusion During laparoscopic cholecystectomy, laparoscopic ultrasonography is helpful for the improvement of correct diagnosis and the decrease of complications.

Objective:To establish the quality control of Chuanqi Zhennaoning capsules.Methods:A TLC method was used to identify Panax Notoginseng and Radix Angelica Pubescentis;an HPLC method was used to determine tetrahydropalmatine in Rhizoma Corydalis (processed with vinegar).Results:The specific spots of Panax Notoginseng and Radix Angelica Pubescentis were presented clearly by TLC without interference from the negative control;the linear range of tetrahydropalmatine was 1.95-24.40 μg·ml-1 (r=0.999 9);the average recovery was 99.2%(RSD=1.51%,n=6).Conclusion:The method is simple,feasible and reproducible,which can be used for the quality control of Chuanqi Zhennaoning capsules.

Objective To explore the diagnostic value of indirect magnetic resonance(MR) arthrography in detecting meniscus tears of the knees.Methods Routine plain MR of the knees followed by indirect MR arthrography was performed in 56 knees with suspected meniscal tears clinically.All cases had subsequently arthroscopic examination or operation.The sensitivity and diagnostic accordance of routine plain MR and indirect MR arthrography were compared based on the result of arthroscopic examination or operation. Results The signal intensity in the area of meniscal tears on image of indirect MR arthrography was obviously higher than that of routine plain MR.The diagnostic accordance rate of indirect MR arthrography was 78.67% while the routine plain MR was 52.20%,and the difference was statistically significant(?~2=21.066,P

AlM:To explore the efficacy and safety of mitomycin C used in trabeculectomy by analyzing intraocular pressure and blood flow.METHODS:A total of 103 patients with glaucoma were divided into 2 groups randomly, and all patients were treated with trabeculectomy, while patients in the observation group were given mitomycin C extra. At 6~12mo follow-up were completed after operation, and the clinical efficacy, complications and hemodynamic characteristics of central retinal artery were compared between groups.RESULTS: The clinical efficacy of the observation group was higher than that of the controls (P<0. 05). There was no significant difference in complication rate between groups (P>0. 05), but the complication type was differentiate to some extent. Low intraocular pressure and photophobia symptoms occurred mostly in the observation group, while cicatricial obstruction occurred mostly in the controls. Before treatment, there was no significant difference in hemodynamic characteristics between groups (P>0. 05). One month after treatment, the peak systolic velocity ( PSV) and end diastolic velocity ( EDV ) of the observation group were obviously lower, while resistance index ( Rl ) and pulsatility index ( Pl ) were higher than those of the controls, the difference was statistically significant ( P<0. 05). Six months after treatment, the PSV and EDV of the observation group were obviously higher, while Rl and Pl were lower than those of the controls, the difference was statistically significant (P<0. 05).CONCLUSlON:Mitomycin C has a short-term harmful effect on glaucoma patients after trabeculectomy, but its long-term effect is safe and reliable.

Objective To explore the pathogenesis and clinical value of Th1/Th2 and normal intestinal flora from the angle of immunological and intestinal micro environment balance by detecting the plasma interferon-γ(IFN-γ)and interleukin-4(IL-4)and fecal bifidobacteria in Children with Allergic Asthma,so as to provide a theoretical basis through early intervention of probiotics to correct the Th1/Th2 balance.Methods The levels of blood plasma IFN-γ,IL-4 and fecal bifidobacteria amount of 165 cases of chil-dren with allergic asthma and 150 health students as control group were detected by ELISA and real time fluorescence PCR at the same time.Retesting these above indexes after applying bifidobacteria regulation of intestinal flora for 6 months.The clinical relative factors and experimental data were statistically analyzed.Results The level of IFN-γof asthmatic children were remarkably lower than those in the control group(P <0.05),while the level of IL-4 of asthmatic children were remarkably higher than those in the control group(P <0.05),and the amount of bifidobacteria of asthmatic children were remarkably lower than those in the control group(P <0.05).The level of IFN-γand bifidobacteria of asthmatic children improved remarkably and the level of IL-4 decreased significantly after applying bifidobacteria regulation of intestinal flora for 6 months.All of them had remarkable statistically signifi-cant.Conclusion The immune balance was affected by the intestinal flora balance of vice versa.This would provide an important theo-retical support to prevent the occurrence of allergic asthma by the early probiotics regulation of intestinal flora and immune balance.

Objective To study the effect of Danggui Buxue Decoction on red cell immune function in mice with morphine abstinence syndrome. Methods 50 mice were randomly divided into normal group,positive control group, high, medium, low dose group,Hindlimb injection of morphine in morphine dependent mice model was established.Erythrocyte membrane were measured in five groups of mice micro viscosity, erythrocyte deformation index; Determination of red cell immune complex rosette rate using the method (CIC), red cell C3b receptor rosette rate;and to observe the five group of regulator factor enhance rate (RFER) and immune adherence inhibitor factor (RFIR) levels.Results Erythrocyte membrane microviscosity in model group were significantly higher than those in normal group, medium group and low group (P<0.05),red cell deformability index was lower than that of normal group, drug medium group and drug group low (P<0.05).Drug high group and medium group,immune complex rosette rate lower than in model group(P<0.05), C3b receptor rosette rate was significantly higher than that in the model group (P<0.05).Conclusion Low dose group and high dose group Danggui Buxue Decoction raises the red cell immune function in morphine withdrawal mice, improves the withdrawal symptoms, rapids rehabilitation of the body, has good clinical value.

Objective To discuss the influence of two recombinant hemoglobin (rHb1.1 and rHb2.0) and human serum albumin (HSA) on oxygen supply and demand balance in rat with coronary heart disease (CHD). Methods Male Wistar rats were randomly divided into normal control group, CHD model group, HSA treatment group, rHb1.1 treatment group and rHb2.0 treatment group, 20 rats in each group. Rat model of CHD was established by high fat diet combined with pituitrin injection. The mean arterial pressure (MAP) decreased to 40 mmHg (1 mmHg = 0.133 kPa) after femoral arterial blood was drawn from the femoral arteries, and the rats were resuscitated with 13.4% HSA, rHb1.1 and rHb2.0, respectively, at the rate of 60 mL·kg-1·h-1 (20 mL/kg). The changes of electrocardiogram (ECG) ST-segment were calculated before model reproduction and at 12 hours after the last time injection of pituitrin. MAP, heart rate (HR), superior mesenteric artery blood flow (QSMA) and arterial blood gas analysis were recorded at 0, 30, 60, 90 and 120 minutes after the administration. The blood was collected after 12-hour fasting, and serum total cholesterol (TC) and triglyceride (TG) were determined by enzymatic method. The pathological changes in cardiac tissue were observed with light microscope. Results Compared with the normal control group, the changes of ECG ST-segment and TC, TG of model group were significantly increased. Compared with the model group, rHb can significantly reduce the value of ST segment changes, and HSA has no such effect; rHb short-term infusion has no significant effect on blood lipids, but can reduce myocardial pathological changes. Compared with the normal control group, the MAP of the model group decreased significantly, the HR was increased, the QSMA was slowed down, the pH value, the residual alkali (BE), the arterial carbon dioxide partial pressure (PaCO2) and HCO3- were decreased significantly. MAP in rHb1.1 group and rHb2.0 group were significantly higher than those in HSA group. Values of MAP were significantly higher in rHb2.0 group than those in rHb1.1 group at 90 minutes and 120 minutes (mmHg: 80.9±3.3 vs. 69.4±4.9, 79.2±4.0 vs. 69.1±3.7, both P < 0.05). The HR of HSA, rHb1.1 and rHb2.0 decreased to normal in 30 minutes after administration, significantly lower than those in the model group (bpm: 534±46, 518±28, 526±37 vs. 609±52, all P < 0.05). In the rHb2.0 group, the QSMA increased significantly at 60, 90 and 120 minutes compared with the model group (qv·mL-1·min-1: 5.6±0.4 vs. 3.9±0.6, 6.2±0.6 vs. 4.1±0.4, 6.9±0.7 vs. 4.0±0.3, all P < 0.05), but there was no significant difference between the HSA group and the rHb1.1 group. The pH, BE, PaCO2 did not return to the normal level after administration of HSA; pH, PaCO2 and HCO3- in the rHb1.1 group returned to normal level at 60 minutes after administration, and BE returned to normal level at 90 minutes after administration. Each index in rHb2.0 group can restore to normal levels 30 minutes ahead of. Conclusion Recombinant hemoglobin can significantly improve the oxygen supply and demand balance of rats with CHD model, can quickly and effectively correct the hypoxic state of blood metabolic acidosis, and rHb2.0 has better effect than rHb1.1.

OBJECTIVE: To explore the effects of Liangxue Huayu therapy (LXHYT), a traditional Chinese herbal therapy for cooling blood and dissipating blood stasis, on rat model of experimental autoimmune thyroiditis (EAT) and its impact on tumor necrosis factor-alpha and interleukin-10 mRNA expressions in peripheral blood mononuclear cells. METHODS: Forty Wistar rats were randomly divided into five groups: normal control group, untreated EAT group, cyclosporine A (CyA)-treated group, Tripterygium glycosides-treated group and Liangxue Huayu Recipe (LXHYR)-treated group. The interventions were given by gavage to the rats in different groups once a day. All rats were sacrificed after 4-week treatment, and the level of serum thyroglobulin antibodies (TgAb) and the changes of histological grade of thyroid specimen were assessed by blind evaluation. Expressions of tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) mRNAs in peripheral blood mononuclear cells were assessed by real-time reverse transcription polymerase chain reaction method. RESULTS: There was a significant increase in the serum TgAb level and severe inflammatory infiltration in the untreated group. Expression of TNF-alpha mRNA in peripheral blood mononuclear cells was increased, while the expression of IL-10 mRNA was decreased in the untreated group (P<0.01). Compared with the untreated group, CyA, Tripterygium glycosides and LXHYF could decrease the serum TgAb level (P<0.05), but the three interventions showed no significant improvement in thyroid inflammation (P>0.05). TNF-alpha mRNA expression was decreased, while IL-10 mRNA expression was increased as compared with the untreated group (P<0.05). CONCLUSION: LXHYT can decrease the serum TgAb level and recover the balance of Th1/Th2. This may provide an experimental basis for further research of assessing the antipsoriatic effect of Chinese herbal drugs with a rat model of EAT as an alternative model of psoriasis in vivo.

Objective To examine the distribution of flurbiprofen axetil in cerebral-spinal fluid (CSF) by determining the CSF concentration of flurbiprofen after iv administration. Methods Seventy-two ASA Ⅰ or Ⅱ patients of both sexes aged 18-75 yr weighing 54-82 kg undergoing spinal or combined spinal-epidural anesthesia for lower extremity or lower abdominal surgery were studied. Flurbiprofen axetil 1 mg/kg was injected intravenously.CSF 2 ml and venous blood 3 ml were obtained simultaneously every 5 min after iv injection for 45 min (T1-9 ) for determination of flurbiprofen concentration using high performance liquid chromatography, and the CSF/blood flurbiprofen concentration ratio was caculated. Results Flurbiprofen was not detected in CSF at T1,2 after iv injection in 3 and 4 patients. The CSF flurbiprofen concentration was significantly higher at T4-9, and CSF/blood flubiprofen concentration ratio higher at T5-9 than at T3 ( P ＜ 0.05). There was no significant difference in CSF flurbiprofen concentrations among T4-9 ( P ＞ 0.05 ) Conclusion Flurbiprofen is detected in CSF after iv injection, the CSF flurbiprofen concentration peaks at 20 min after iv injection and it lasts until 45 min after iv injection.