Objective To investigate the association of the serum levels of ischemia-modified albumin（IMA）and homocysteine（Hcy）with the onset and severity of white matter ischemic lesions（WMIL）. Methods A total of 89 patients who were diagnosed with WMIL by cranial magnetic resonance imaging（MRI）in our hospital from March 2020 to June 2022 were enrolled as WMIL group，and the volunteers with normal cranial MRI results，matched for sex and age，during the same period of time were enrolled as control group. The two groups were compared in terms of general information and laboratory markers，and the conditions of WMIL were evaluated and classified into mild and moderate-to-severe cases. A logistic regression analysis was used to investigate the influencing factors for the onset and severity of WMIL，and the receiver operating characteristic（ROC）curve was used to analyze the value of the serum levels of IMA and Hcy in the diagnosis of WMIL. Results Compared with the control group，the WMIL group had a significantly higher proportion of patients with hypertension or type 2 diabetes and significantly higher levels of uric acid（UA），IMA，and Hcy（P<0.05）. Compared with the control group，the WMIL group had a significantly higher proportion of the patients with moderate-to-severe WMIL who had hypertension or the proportion of type 2 diabetes，significantly higher levels of IMA and Hcy，and a significantly lower level of high-density lipoprotein cholesterol（P<0.05）. The logistic regression analysis showed that hypertension，elevated IMA level，and elevated Hcy level were influencing factors for the onset of WMIL（P<0.05），and hypertension，elevated IMA level，and elevated Hcy level were influencing factors for the increase in WMIL severity（P<0.05）. The ROC curve analysis showed that serum IMA and Hcy had a certain value in the diagnosis of WMIL and moderate-to-severe WMIL. Conclusion Increases in the serum levels of IMA and Hcy are associated with the onset and aggravation of WMIL，and measurement of the serum levels of IMA and Hcy has a certain value in the diagnosis of WMIL and moderate-to-severe WMIL.
Homocysteine

No abstract available.
Atherosclerosis* ; Homocysteine*

Background: Preeclampsia is a major cause of maternal and perinatal mortality and morbidity, affecting 5 - 6% of all pregnancies. Recently, homocysteine (Hcy), a metabolite of amino acid methionine has been postulated producing oxidative stress, endothelial cell dysfunction, and alterations associated with preeclampsia. It is unclear whether high concentration of circulating Hcy causes preeclampsia, or whether this is a secondary phenomenon of metabolic alterations resulting from the disorder. Objectives: (1) Determining blood Hcy concentration in pregnancies in various severities of preeclampsia. (2) Discover the relationships between serum Hcy and other biological markers in preeclampsia. Subjects and method: This descriptive cross-sectional study consisted of 3 groups of pregnancies admitted to Thanh Nhan Hospital: 24 normal pregnant women, 28 pregnancies with non-serious preeclampsia, and 27 pregnancies with serious preeclampsia. Concentrations of blood Hcy of all participants were assayed by a competition fluorescence immunoassay (FPIA). Results: The mean concentration of serum Hcy during normal pregnancy was 5.2+/-1.0micromol/L compared with 7.1+/-1.8micromol/L among pregnancies with non-serious preeclampsia, and 11.7+/-2.9micromol/L among pregnancies with serious preeclampsia. Serum Hcy increased in pregnancies with renal dysfunction, elevated serum uric acid, and injuries of liver cells. Conclusion: Concentration of the serum Hcy in pregnancies with serious preeclampsia is significantly higher than that of pregnancies with non-serious preeclampsia, and the serum Hcy in pregnancies with non-serious preeclampsia is significantly higher than that of normal pregnancies. There are relationships between elevated serum Hcy in preeclampsia with level of kidney failure, injury of liver and increased levels of serum uric acid.
Homocysteine ; Preeclampsia

Objective To explore the value of serum homocysteine​（HCY） and cystatin C （Cys-C） levels for evaluating the presence and severity of cognitive impairment in elderly patients with both cerebral small vessel disease （CSVD） and obstructive sleep apnea-hypopnea syndrome （OSAHS）.Methods A retrospective study was performed on 150 elderly patients with both CSVD and OSAHS who were treated in our hospital from January to December 2020. The data about HCY and Cys-C levels were collected. According to the presence or absence of cognitive impairment， they were divided into observation group （with cognitive impairment， 41 cases） and control group （without cognitive impairment， 109 cases）. The serum HCY and Cys-C levels of the two groups were compared. The correlations between serum HCY and Cys-C levels and cognitive impairment in patients with both CSVD and OSAHS were analyzed. A binary Logistic regression analysis was performed to determine independent factors affecting cognitive impairment to establish a predictive logistic regression model. Receiver operating characteristic （ROC） curves were plotted to examine the predictive value of HCY， Cys-C， and their combination for cognitive impairment in patients with both CSVD and OSAHS. According to the severity of cognitive impairment， the patients in the observation group were further divided into mild cognitive impairment group （32 cases） and severe cognitive impairment group （9 cases） to compare HCY and Cys-C levels and analyze the value of HCY， Cys-C， and their combination in evaluating the staging of cognitive impairment by using ROC curves.Results The levels of HCY and Cys-C in the observation group were significantly higher than those in the control group （both P<0.05）. The total score of Montreal Cognitive Assessment was negatively correlated with serum HCY and Cys-C level （r=-0.756， -0.713， both P<0.001）. The logistic regression analysis showed that HCY and Cys-C levels were independent influencing factors for cognitive impairment （both P<0.05）. The area under the curve （AUC） of HCY plus Cys-C for prediction was 0.843（0.747-0.934）， which was higher than that of any single indicator （P < 0.05）， with the sensitivity and specificity being 89.32% and 79.25%， respectively. The levels of HCY and Cys-C were significantly lower in the patients with mild cognitive impairment than in those with severe cognitive impairment （both P < 0.05）. The AUC of HCY plus Cys-C in cognitive impairment staging was 0.925（0.849-0.976）， which was higher than that of any single index （P < 0.05）. The sensitivity and specificity were 95.67% and 89.67%， respectively.Conclusion Serum HCY and Cys-C levels are negatively correlated with the development and progression of cognitive impairment in elderly patients with both CSVD and OSAHS. The combined prediction with the two indicators can facilitate the evaluation of the risk and stage of cognitive impairment.
Homocysteine ; Cystatins

Objective To investigate the risk factors for the early progression of atherosclerosis，whether hyperhomocysteinemia （HHcy） is an independent risk factor for the early progression of atherosclerosis， and whether the reduction in homocysteine （Hcy） can delay the early progression of atherosclerosis. Methods The villagers，aged ≥40 years， from Liulin Town of Hanzhong City， Shaanxi Province， China were selected for structured inquiry， carotid ultrasound examination， and blood test in May 2017 and June 2020. Ultrasound examination was performed to measure carotid intima-media thickness （CIMT）， carotid plaque， and carotid stenosis rate to reflect the degree of arteriosclerosis. Results A total of 500 individuals were included in the final analysis， among whom 451 had no CIMT thickening at baseline， 429 had no carotid plaque at baseline， and 454 had no carotid stenosis at baseline. After 3 years of follow-up， among the 500 individuals， 176 （35.2%） had CIMT thickening， and the multivariate analysis showed that old age， smoking， and a high level of low-density lipoprotein cholesterol （LDL-C） were independent risk factors for CIMT thickening； 252 （50.4%） had new-onset plaques， and the multivariate analysis showed that old age， hypertension， and high LDL-C were independent risk factors for plaque formation； 231（46.2%） had new-onset carotid stenosis， and the multivariate analysis showed that old age， high LDL-C， and elevated triglyceride were independent risk factors for carotid stenosis. There were 364 patients with HHcy （≥15 μmol/L） in the initial examination， and after 3 years， 119 had a reduction in Hcy， while 245 had no reduction in Hcy. After adjustment for sex， previous stroke， smoking， baseline LDL-C， baseline Hcy， and Hcy after 3 years， the multivariate analysis showed that neither HHcy nor the reduction in Hcy was associated with carotid atherosclerosis. Conclusion Old age， high LDL-C， smoking， and hypertension are the main risk factors for atherosclerosis in villagers aged ≥40 years in rural areas of Hanzhong City in Shaanxi Province. There is no significant association between HHcy and carotid atherosclerosis， and the reduction in Hcy cannot delay or reverse the process of carotid atherosclerosis.
Atherosclerosis ; Homocysteine

68 patients with stroke treated at Hue Central Hospital from March to June 2004. Risk factors as hypertension, smoking were evaluated. Patients had clinical and paraclinical examination such as CT scanning of cerebral skull, blood formula. Blood level of homocystein was estimated: The prevalence of stroke in gender male/female was 1.64/1 with 61.76% men and 38.24 women. Hypertension 85.24%, men/women 1.64/1. Smoke 67.65%. Blood level of homocystein increases in stroke by the rate of 77.94%, as for hemorrhagic stroke was 65.63%, for ischemic stroke was 88.57%. Stroke rate increased by age, especially after 55 years old. A positive correlation between blood level of homocystein and hematocrite was notified
Stroke ; Homocysteine ; blood

Over the last decade there is strong evidence indicating that hyperhomocysteinemia appears to be an important risk factor for vascular diseases, including cerebrovascular disease and myocardial infarction. Several multicenter-randomized trials have demonstrated that it is independent to other well known risk factors such as hypertension, diabetes mellitus, hyperlipidemia and smoking. Given the intense interest of our community concerning the prevention of cerebrovascular diseases we begin to measure homocysteinemia with a simple and accurate technique. The first results of our study raise the exciting possibility that homocysteinemia levels can be normalized by an inexpensive, well tolerated therapy with folate, pyridoxine hydrochloride and cyanocobalamin.
Cerebrovascular Disorders ; Homocysteine

The concentration of blood homocystein (Hcy) in 26 patients with cerebrovascular accident (CVA) was determined preliminarily in Central Hospital in Hue city. 57.69% was the incidence of CVA in male and 42.31% in female patients (in male it was 1.36 fold higher than in female). Hcy concentration increased in 80.77% CVA patient. The rate of increase of blood level of Hcy was 66.67% in brain hemorrhage, 92.86% in brain infarctus. The incidence of CVA enhanced with the age, especially after the age of 55. High blood pressure accounted for 76.92% in CVA. 17/26 (65.38%) was the ratio of tobacco smoler.
Data Collection ; Homocysteine ; Blood ; Stroke

No abstract available.
Folic Acid* ; Homocysteine* ; Methylenetetrahydrofolate Reductase (NADPH2)*

BACKGROUND: The aim of this study was to investigate alterations in homocysteine levels with delays in the initiation time of centrifugation. Attempts were made to improve the processing of samples by analyzing the homocysteine levels of patients from a health promotion center, where delays in the initiation of sample centrifugation were expected because of the nature of the workload and operational procedures. METHODS: Forty healthy adults were selected for the measurement of homocysteine levels. The two samples those were obtained from each individual simultaneously and separately were designated as group A (centrifugation initiation time < or =20 minutes after blood sampling) and group B (> or =2 hours). The degree of deviation from the homocysteine reference interval was also evaluated with samples from 1,134 adults who had medical check-up at a health promotion center from August 1, 2009 to July 31, 2010. RESULTS: The mean serum homocysteine level in group B was significantly higher than that of group A (12.4 vs. 10.6 micromol/L, P<0.0001). Homocysteine level increased with a mean of 19.4% when the initiation of centrifugation was delayed over 2 hours. In the 1,134 adults who had medical check-up, the numbers outside the reference interval were 334 (29.5%). Abnormal samples with homocysteine levels out of the reference interval were reduced to about 7% in July 2011 through improving sample processing to minimize delays in centrifugation initiation time. CONCLUSIONS: Homocysteine levels rapidly increase as blood sample centrifugation is delayed. Therefore, in order to provide accurate test results immediate centrifugation of blood samples is critical.
Adult ; Centrifugation ; Health Promotion ; Homocysteine ; Humans