In patients with cleft palate, ear disease and hearing loss have long been recognized as common problems. In this report, otitis media with effusion and atelectasis were presented at otoscopy and tympanoplasty in 76.4% of 170 infants and children with cleft palate. This rate changes by age and there is not big different between male and female
Cleft Palate ; Child

A retrospective study on 77 cases of injuries in the neck in the institute of ear, nose and throat during 1970-1994 has shown that the rate of disease free among patients who early hospitalized without complication was 91.6%. The rate of sequela due to the pharyngeal cartilage rupture was 8.41%. The rate of disease free, sequela and mortality among patients who lately hospitalized with complication was 54.5%, 27.3% and 18.2% respectively
Neck ; Neck Injuries

D1S80 locus is highly polymorphic and has been used worldwide as an important marker for forensic, medical analysis and paternity tests. Vent DNA Polymerase is a high-fidelity thermophilic DNA polymerase. The fidelity of Vent DNA polymerase is 5 to 15 folds higher than that observed for Tag DNA polymerase. This high fidelity derives in part from an integral 3’-5’ proofreading exonuclease activity in Vent DNA polymerase. Greater than 90% of the polymerase activity remains after one hour incubation at 95oC. Because of these advantages, we want to apply Vent DNA polymerase for the amplification of D1S80 alleles by PCR. Our study showed that PCR condition for Vent DNA polymerase are rather different than for Tag DNA polymerase. The amplification of D1S80 alleles with Vent DNA polymerase is optimal with the following parameters: 1. A final concentration of MgSO4 in PCR mixture of 4 mM. 2. An annealing temperature for the specific D1S80 primers 68oC. 3. A final concentration of dNTPs of 200mcM.
DNA polymerase ; Alleles

Study was carried out at some kindergarten with 773 children of 1-5 years old of age. Results showed an incidence of catarrhal media otitis of 10.34%. This incidence trends to increased in the lower age with a peak of 12.21% at the age of 2, then diminished gradually. The gender rate is 53.75% in boys comparing with 46.25% in girls. In 75.60% patients the condition occurred in both sides of two ears and in the case of unilateral development, the rate is 70.27% in the left in comparing with 29.73% in right ear
Otitis Media ; Child ; Epidemiologic Studies ; epidemiology

Study performed with 1168 children aged 1-15 in some kindergarten and maternal school, primary school and secondary school in the commune Trung Tu, Kim Lien – District Dong Da – Ha Noi had showed that 8,9% children had effusive otitis media. In the group of children with VA, the incidence of effusive otitis media was higher than in the group without VA (13,74% vs 8,01%). In the group of children with tonsilitis, the incidence of effusive otitis media was higher than in the group without tonsilitis (15,69% vs 8,26%)
Otitis Media with Effusion ; Respiratory Tract Infections ; Child

Injection-related necrosis is a common pattern in the arterial injuries. But the acute embolization that led to extremity necrosis is very rare. Here we described a case of 43-year male patient with arm necrosis due to intra-arterial drug injection. The patient has addicted drug for 24 years and has injected opium for 18 years with dose of 2 ml self-prepared opium solution, 3-4 times daily. On April 2000, after second injection of a day, the patient felt burn and pain in his left hand. Then watery purples and necrotic papules appeared on the skin of left arm. He was treated by antibiotics, analgesics, and vasodilators, but the condition was not improved. The patients had to be amputated his left arm after 3 days of admission.
Substance Withdrawal Syndrome ; Alcohol Withdrawal Delirium ; diagnosis

During 5 months from 1/2000 to 5/2000, at Nhan Dan Gia Dinh Hospital, we have operated 5 patients with post-traumatic rupture of diaphragm. Among them, three planned operations and two urgent operations. All cases were diagnosed by clinical findings associated with chest X ray and GI tract examinations. Only one case we had by thoracotomy, the rest had been performed by laparotomy. The realized target of this study is determining the typical semiology of diaphragmatic rupture and proposing the laparotomy access for delayed diaphragmatic rupture.
Rupture ; Diaphragm

Deep vent DNA polymerase is purified from a strain of E. Coli that carries the Deep Vent DNA polymerase gene from pyrococcus species GB-D. The native organism was isolated from a submarine thermal vent at 2010 meters and is able to grow at temperature as high as 104oC. Deep Vent DNA polymerase is a high-fidelity thermophilic DNA polymerase. The Deep Vent DNA polymerase is derived from an integral 3'-5' proofreading exonuclease activity. Deep Vent DNA polymerase is ven more stable than Vent DNA polymerase at temperature of 95 to 100oC. Because of these advantages, we want to apply Deep Vent DNA polymerase for amplification of NS4 gene - hepatitis C virus by PCR. Our study showed that PCR conditions for Deep Vent DNA polymerase are rather different than for Taq DNA polymerase. The amplification of NS4 gene - hepatitis C virus with Deep Vent DNA polymerase is optimal with the following parameters: (1) A final concentration of MgSO4 in PCR mixture of 4 mM. (2) An annealing temperature of 57oC. (3) A final concentration of dNTPs of 200 mM.
Hepatitis C ; Deep Vent DNA polymerase

Study on blood samples of 29 patients with hemophilia A and 25 normal people to determine the mutation of intron 18 and 19 of factor VIII by PCR-RFLP using Vent DNA polymerase assay. PCR and RFLP methods were used. Results: 16/29 (55%) of haemophilia A patients has the mutation in intron 18 of factor VIII gene and none of them has the mutation in intron 19. This PCR-RFLP assay can be applied for detection of mutations and these methods can be used for carrier analysis and prenatal diagnosis of hemophilia A.
Hemophilia A ; Polymerase Chain Reaction ; DNA

Beta-Thalassemia is a blood disease with beta-globin deficiency caused by a directly down-regulation in the synthesis of structurally normal beta chains. Vent DNA polymerase is a high-fidelity thermophilic DNA polymerase, and the fidelity 5 - 15 times higher than Taq DNA polymerase (1/31,000 vs. 1/290 - 1/2,400). Using ARMS PCR (Amplification Refractor Mutation System-PCR) with Vent DNA polymerase, we found that 9/28 (32%) of the tested beta-Thalassemia patients had the mutation at codon 17 and 4/28 (14%) at codon 41/42. This method can be applied for a rapid prenatal diagnosis of beta-Thalassemia disease and has public health significance.
beta-Thalassemia ; DNA ; Polymerase Chain Reaction ; Diagnosis