The author had reported that laser irradiation with energy density 4J/cm² at every other days was most effective for wound healing.
But there is a report which described that laser irradiation with energy density under 4J/cm² was effective for wound healing too.
Then, the effect of irradiation with under 4J/cm² He-Ne laser on wound healing was studied. Twenty-four wister rats were subjected to this experiment.
A open wound, 20mm in diameter as deep as muscle layer were made on the back of anesthetized animals.
To find the suitable energy density of laser irradiation, the wound was irradiated with He-Ne laser by energy density of 4J/cm², 2J/cm² and 1J/cm2 every other day until the wound completely healed.
The wound healing was much more accelerated by irradiation with energy density of 4J/cm² than the other group.
This result suggested that laser irradiation with energy density 4J/cm² at every other days is most effective for wound healing.

The specificity of Jin-yu needling for urinary secretion was studied.
1); 15 healthy adult men were subjected to this experiment. After drinking water (500ml), acupuncture needles (No. 2 Seirin Co. Limited) were perpendiculary inserted to the jin-yu point depth of about 2cm, and left there for 10 minutes. The blood samples were collected at 60 minutes after drinking water, and urine volume and amount of Na, K, Cl, BUN, UA and Cre were measured.
As a result, a marked increase was observed in excretion of urine, UA, Cre, K and creatinine clearance. An upward tendency of Na, Cl and BUN excretion were found and it is correlated with urine volume.
2); Subjects were 11 healthy adult men. The group of acupuncture stimulation was divided as follows; a) a group, acupuncture needles were inserted at Jin-yu point [B-23], b) kyouseki point, c) sisitu point [B-52], d) daityou-yu point [B-25], e) I-yu point [B-21] and f) untreated contorol group. Urine were collected at 60 minutes after drinking water (500ml).
As a result, a significant increase of urine volume was observed in the group of stimulation at Jin-yu, Kyouseki, and Sisitu point (p<0.05).
Finally, it was suggested that the increase of urine volume was caused by a somato-visceral reflex.

The effect of irradiation with low power He-Ne laser on wound healing was studied. 42 wister rats were subjected to this experiment.
A open wound, 20mm in diameter as deep as muscle layer were made on the back of anesthetized animals.
To find the suitable energy density of laser irradiation, the wound was irradiated with He-Ne laser by energy density of 4J/cm² or 10J/cm² every other day until the wound completely healed. The wound healing was much more accelerated by irradiation with energy density of 4J/cm² than that of 10J/cm² and none-irradiated contorol group.
To find the suitable interval of laser irradiation, the wound was irradiated every other days, every 4 days or every other days in early stage until the wound completely healed. From this study, the wound healing was much more acceralated by irradiation at every other days than the others.
This result suggested that laser irradiation with energy density 4J/cm² at every other days is most effective for wound healing.