1.CHANGES IN HEAT SHOCK PROTEIN INDUCTION IN RAT SKELETAL MUSCLES FOLLOWING ACUTE TREADMILL TRAINING AT DIFFERENT SPEEDS
KAZUHIRO TODOROKI ; TAKAO SUGIURA ; KATSUMASA GOTO ; HISASHI NAITO ; TOSHITADA YOSHIOKA
Japanese Journal of Physical Fitness and Sports Medicine 2004;53(5):537-547
It has been demonstrated that exercise induces heat shock proteins (HSPs) . However, no study has investigated changes in HSPs following endurance training at different speeds. Therefore, this study was designed to investigate the effect of treadmill training at different running speeds on induction HSPs. One group of male Wistar rats was assigned as a sedentary control, three groups were assigned for exercise training (10 m/min, 20 m/min and 30 m/min) and another three groups for one acute bout of exercise (10 m/min, 20 m/min and 30 m/min) . Each training group ran at each speed for 30 min/day, 5 days a week for 8 weeks. The acute exercise group performed the exercise only once. Forty-eight hours after the last exercise session was completed, the rats were sacrificed and the plantaris (PLA) and soleus (SOL) muscles were dissected. In the acute exercise group, the content of HSP72 in both the PLA and SQL increased (p<0.05) at all speeds, and the content of HSP60 increased significantly (p<0.05) at all speeds for the PLA, but not for the SOL. On the other hand, in the endurance group, the content of HSP72 and HSP60 in both muscles increased in 30 m/min groups. These results indicate that an increase in HSP72 and HSP60 by endurance training is induced by high intensity training in both muscles. This was not found to be the case with the acute exercise groups.
2.EFFECT OF HEAT STRESS ON DESMIN EXPRESSION IN ATROPHIED SOLEUS MUSCLE
TAKAO SUGIURA ; TSUBASA SHIBAGUCHI ; TOSHINORI YOSHIHARA ; YUKI YAMAMOTO ; KATSUMASA GOTO ; HISASHI NAITO ; TOSHITADA YOSHIOKA
Japanese Journal of Physical Fitness and Sports Medicine 2010;59(1):167-174
In the present study, we investigated the effect of heat stress on disuse atrophy from changes in the muscle protein levels of desmin and calpain. Wistar strain female rats (6-8 months old) were randomly assigned to two experimental groups: control (C) and heat stress (H). One hindlimb of all animals was immobilized in plantar flexion with plaster. Before immobilization, animals in H group were placed in a heat chamber (42°C for 60 min). Following 6 days of immobilization, the soleus muscles were removed and analyzed. Although immobilization resulted in significant muscle atrophy in all experimental animals, the soleus weight-to-body weight ratio in immobilized limbs of H group was significantly higher compared to that of C group. Expression of desmin and HSP72 in the atrophied soleus muscle from C group was significantly lower compared with the contralateral muscle; but this was not the case in H group. Further, in C group, the ratio of autolyzed calpains I increased significantly in the atrophied muscle compared to the contralateral muscle. These results show that the effect of heat stress on disuse skeletal muscle atrophy is attributed to the decreasing degradation of desmin by suppressing the activation of calpain.
3.EFFECT OF LONG-TERM DIETARY ASTAXANTHIN INTAKE ON SARCOPENIA
TSUBASA SHIBAGUCHI ; TAKAO SUGIURA ; TSUKASA FURUMOTO ; KOSHIRO INOUE ; YOSHIHARU IIDA ; TOMOMI ISOYAMA ; HISASHI NAITO ; KATSUMASA GOTO ; DAIJIRO OHMORI ; TOSHITADA YOSHIOKA
Japanese Journal of Physical Fitness and Sports Medicine 2008;57(5):541-552
Oxidative stress is thought to be a significant contributing factor of age-related sarcopenia. We tested the hypothesis that long-term dietary antioxidant (astaxanthin) intake attenuates sarcopenia. Wistar strain male rats, aged 45 weeks old, were given either control (Cont) or astaxanthin feed (0.004%, Ax) for 1 year. The soleus muscle weight and muscle weight-to-body weight ratios in Ax group were significantly higher than in Cont group, but tibialis anterior muscle mass was similar between the two dietary groups. The level of ubiquitinated proteins was significantly lower in the soleus muscles of Ax group, but not in tibialis anterior muscles when compared with Cont group. Tibialis anterior levels of cathepsin L, especially, and caspase-3 tended to be lower in Ax group than in Cont group. Cathepsin L levels were significantly lower. Whereas no differences between Cont and Ax were observed in soleus levels. There were no significant differences in Ax supplementation on calpain 1 and 2, UBC3B, Cu/Zn SOD and nitrotyrosine levels in either soleus or tibialis anterior muscles. Our data suggest that long-term dietary astaxanthin intake attenuates age-related muscle atrophy, due in part, to reduction in ubiquitination of myofibrillar protein in slow soleus muscles, but not in fast tibialis anterior muscles.
4.EFFECTS OF COMBINATION OF HEAT STRESS AND ASTAXANTHIN SUPPLEMENTATION ON DISUSE MUSCLE ATROPHY
TOSHINORI YOSHIHARA ; TAKAO SUGIURA ; TSUBASA SIBAGUCHI ; YUKI YAMAMOTO ; KATSUMASA GOTO ; TOMOMI ISOYAMA ; HISASHI NAITO ; DAIJIRO OHMORI ; TOSHITADA YOSHIOKA
Japanese Journal of Physical Fitness and Sports Medicine 2010;59(3):303-312
The purpose of this study was to examine the effects of combination of a heat stress and astaxanthin supplementation, known as a potent anti-oxidative nutrient, on muscle protein degradation and disuse muscle atrophy. Fifty-two male Wistar rats (261.7±1.17 g) were divided into five groups: control (Cont, n=10), suspension (Sus, n=11), heat stress and suspension (Heat, n=10), astaxanthin and suspension (Ax, n=10), and heat stress, astaxanthin and suspension (H+A, n=11). There were no significant differences in Cu,Zn-SOD, cathepsin L and caspase-3 levels among the Heat, Ax and H+A groups in the soleus and plantaris muscles. Although levels of calpain 2 and ubiquitinated protein in the myofibrillar fraction in the soleus muscle were not significantly different among the Heat, Ax and H+A groups, levels in the H+A group were significantly (p<0.05) lower than Sus. Concerning atrophied plantaris muscles, the H+A group significantly (p<0.05) suppressed the expression of calpain 1 in the myofibrillar fraction, but there were no marked changes of proteolytic indexes. These data indicate that the combination of the heat stress and astaxanthin supplementation could be effective in inhibiting muscle protein degradation in disuse atrophy of the soleus.
5.Improvement Effects of Dentifrice Containing Plant Extracts on Periodontal Disease
Koji HATTORI ; China SATO ; Hiroshi TAKAGI ; Youichi YASHIRO ; Hisashi GOTO ; Yuki SUZUKI ; Genta YAMAMOTO ; Akio MITANI ; Satoru NAKATA
Japanese Journal of Complementary and Alternative Medicine 2017;14(1):27-32
Effects of three plant extracts (Hordeum vulgare L., Apocynum Venetum L., Brasenia schreberi J.F.G mel.) on human gingival fibroblasts were examined. As a result, we observed the promoting effect of the extract of Hordeum vulgare L. and the extract of Apocynum Venetum L. respectively on FGF2 and FGF7 production. Moreover, the mixture of the three plant extracts showed the effect of improving the changes in type I collagen gene expression and matrix metalloproteinase 1 gene expression by LPS addition. Next, a dentifrice containing the three plant extracts was subjected to human efficacy trials. We measured periodontal pocket depth, attachment level, bleeding on probing and saliva TNFα as an indicator of periodontal disease. The results suggest that the dentifrice formulated with the three plant extracts were effective for the improvement of periodontal disease.
6.A Case of Hyperandrogenism, Insulin Resistance, and Acanthosis Nigricans Syndrome; Increase in Proliferating Cell Nuclear Antigen and Decrease in Loricrin in Acanthosis Nigricans.
Kanami SAITO ; Hisae ANDO ; Koro GOTO ; Tetsuya KAKUMA ; Yasushi KAWANO ; Hisashi NARAHARA ; Yutaka HATANO ; Sakuhei FUJIWARA
Annals of Dermatology 2016;28(5):637-639
No abstract available.
Acanthosis Nigricans*
;
Hyperandrogenism*
;
Insulin Resistance*
;
Insulin*
;
Proliferating Cell Nuclear Antigen*
7.A Case of Hyperandrogenism, Insulin Resistance, and Acanthosis Nigricans Syndrome; Increase in Proliferating Cell Nuclear Antigen and Decrease in Loricrin in Acanthosis Nigricans.
Kanami SAITO ; Hisae ANDO ; Koro GOTO ; Tetsuya KAKUMA ; Yasushi KAWANO ; Hisashi NARAHARA ; Yutaka HATANO ; Sakuhei FUJIWARA
Annals of Dermatology 2016;28(5):637-639
No abstract available.
Acanthosis Nigricans*
;
Hyperandrogenism*
;
Insulin Resistance*
;
Insulin*
;
Proliferating Cell Nuclear Antigen*
8.Effects of a Communication Program in Undergraduate Medical Education on Physician's Clinical Practice.
Tatsuro ISHIZAKI ; Yuichi IMANAKA ; Akihiro OKAMOTO ; Hisashi OKUYAMA ; Yasuaki KAMANO ; Takahiro KIUCHI ; Satoshi GOTO ; Takeshi TANIGAWA ; Takeo NAKAYAMA ; Satoshi HONJO ; Shunsaku MIZUSHIMA ; Noriko MURAKAMI
Medical Education 1998;29(6):399-406
This study examined the effectiveness of a communication program in undergraduate medical education in improving communication in physicians' clinical practice. The effectiveness of the program was assessed with a mail survey using self-rated questionnaires 9 years later. Ninety participants were follwed up in late 1994; 57.8% of them replied to the questionnaire. Of the respondents, 60% replied that programs concerned with active listening and role-playing had benefits on communicating with patients and families. In addition, 40% of respondents answered that case studies aimed at teaching comprehensive medicine with the team approach was effective in improving communication with co-medical staff. These results suggest that the communication program in undergraduate medical education is effective in improving clinical communication in clinical practice when students are highly motivated.
9.Studies on Therapeutic Effects and Pathological Features of an Antithrombin Preparation in Septic Disseminated Intravascular Coagulation Patients.
Yuichiro SAKAMOTO ; Satoshi INOUE ; Takashi IWAMURA ; Tomoko YAMASHITA ; Atsushi NAKASHIMA ; Yoichi NISHIMURA ; Hiroyuki KOAMI ; Hisashi IMAHASE ; Akiko GOTO ; Kosuke Chris YAMADA ; Kunihiro MASHIKO ; Hiroyuki YOKOTA
Yonsei Medical Journal 2013;54(3):686-689
PURPOSE: Few reports have been made on the therapeutic effects as well as pathological features of an antithrombin preparation in patients diagnosed with septic disseminated intravascular coagulation (DIC) by the diagnostic criteria for acute DIC. MATERIALS AND METHODS: A total of 88 sepsis patients who had received inpatient hospital care during the period from January 2000 through December 2008 were divided into two groups, an antithrombin group and a non-antithrombin group, to study the outcomes. Furthermore, the relationship between sepsis-related factors and DIC in 44 patients was studied. RESULTS: The antithrombin group contained 34 patients, and the non-antithrombin group contained 54 patients. The outcomes were significantly better in the antithrombin group. The levels of protein C were low in DIC patients. CONCLUSION: Our results suggest that early administration of antithrombin might improve outcomes of septic DIC patients in the diagnostic criteria for Japanese Association for Acute Medicine acute DIC.
Aged
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Disseminated Intravascular Coagulation/complications/diagnosis/*drug therapy
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Female
;
Fibrinolytic Agents/*therapeutic use
;
Humans
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Male
;
Middle Aged
;
Sepsis/complications/diagnosis/*drug therapy
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Time Factors
;
Treatment Outcome
10.Usefulness of the Endotoxin Activity Assay to Evaluate the Degree of Lung Injury.
Yuichiro SAKAMOTO ; Satoshi INOUE ; Takashi IWAMURA ; Tomoko YAMASHITA ; Atsushi NAKASHIMA ; Hiroyuki KOAMI ; Toru MIIKE ; Mayuko YAHATA ; Hisashi IMAHASE ; Akiko GOTO ; Showgo NARUMI ; Miho OHTA ; Chris Kosuke YAMADA
Yonsei Medical Journal 2014;55(4):975-979
PURPOSE: It has been reported that the Pulse Contour Cardiac Output (PiCCO) is very useful mainly in the field of intensive care and treatment to grasp the pathophysiological conditions of pulmonary edema because of its capability of obtaining data such as Pulmonary Vascular Permeability Index (PVPI) and Extra Vascular Lung Water (EVLW). Furthermore, a high degree of usability of various markers has been reported for better understanding of the pathological conditions in cases with septicemia. MATERIALS AND METHODS: The correlation between the cardiorespiratory status based upon the PiCCO monitor (EVLW and PVPI) and inflammatory markers including C reactive protein, procalcitonin (PC), and Endotoxin Activity Assay (EAA) were evaluated in 11 severe cases that required treatment with a respirator in an intensive care unit. RESULTS: The EAA values were significantly higher in patients with abnormal EVLW at 0.46+/-0.20 compared to the normal EVLW group at 0.21+/-0.19 (p=0.0064). In a similar fashion, patients with abnormal PVPI values tended to have higher PC levels at 18.9+/-21.8 compared to normal PVPI cases at 2.4+/-2.2 (p=0.0676). On the other hand, PVPI was significantly higher in the abnormal EAA group at 3.55+/-0.48 in comparison with the normal EAA group at 1.99+/-0.68 (p=0.0029). The abnormal EAA group tended to have higher PVPI values than the normal EAA group. CONCLUSION: The EAA is a measurement method designed to estimate the activity of endotoxins in the whole blood. Our results suggest that the EAA value, which had the greatest correlation with lung disorders diagnosed by the PiCCO monitoring, reflects inflammatory reactions predominantly in the lungs.
Adult
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Aged
;
Aged, 80 and over
;
Cardiac Output/physiology
;
Endotoxins/*blood
;
Female
;
Humans
;
Lung Injury/*blood/*diagnosis/physiopathology
;
Male
;
Middle Aged
;
Pulmonary Edema/blood/*diagnosis/physiopathology