Some fragments of gravid proglottids of diphyllobothriid cestodes were evacuatedspontaneously, and complete strobila with scolex was expelled after the treatment with bithionol from a 35-year-old man in Ehime Prefecture, Where diphyllobothriasis has scarcely been recorded. The cestodes was identified as Diphyllobothrium latum by the following morphological characters: thin, weakly muscled strobila, with clavate scolex: distal end of uterus extending posteromediad to uterin pore, forming an acute angle with ventral surface of segment, genital pore and uterine pore widely separated; vagina turning abruptly ventrad just ventral to seminal vesicle, forming an angle of near 60°; there is a constriction between segments and often an area without testes and vitellaria; ovary with no anterior horns seen; the smooth surface of the egg-shell with shallow and widely spaced pits (scanning electron micrograph)

Objective To study H2O2-induced single strand breaks(SSB) formation in DNA and the reparation in NIH3T3 mouse fibroblasts by single cell gel electrophoresis technique(SCGE,Comet Assay) and to introduce SCGE.Methods The NIH3T3 mouse fibroblasts were incubated,and cell damage was induced by H2O2 and determined by SCGE.Results ① H2O2-induced damage class graph of the DNA Comet in the NIH3T3 mouse fibroblasts was established.② H2O2-induced SSB was H2O2 dose-dependent.③ The reparation of the damaged DNA was time-dependent and could be achieved 15min after incubation.The data indicated that most reparations were completed within 1 hour,but a few might take longer time.Conclusion Single cell gel electrophoresis technique is a simple and sensitive method to determine oxidation-induced single strand breaks(SSB) formation in DNA.

Introduction: We report a case of severe headache caused by lung cancer metastasis to the base of the skull that was difficult to diagnose due to a lack of imaging evidence. Case: A 70-year-old man diagnosed with advanced lung cancer experienced sudden, severe headache. He was diagnosed as having a tension-type headache because magnetic resonance imaging of his head failed to detect any pathology. He was prescribed various drugs, which except for strong opioids failed to treat his headache. He referred to our palliative care unit to treat the pain. Re-evaluation of his head CT revealed metastasis to the clivas. His pain was treated with rapid titration of subcutaneous oxycodone injection. Conclusion: Even if radiographic investigations fail to identify the metastasis, the patient should be re-evaluated if the headache worsens and/or is accompanied with cranial nerve dysfunction.

We evaluated the surgical strategy for thoracic aortic aneurysm associated with abdominal aortic aneurysm. From January 1982 to March 1999, 24 consecutive patients underwent surgical treatment for thoracic aortic aneurysm with abdominal aortic aneurysm. Staged operation was performed if one was only slightly dilated, but extensive operation was needed if the size of both aneurysms was greater than 6cm. In cases of thoracic aortic aneurysm with abdominal aortic aneurysm up to 4cm in size, surgical treatment was performed only for the thoracic aortic aneurysm. Circulatory support during operation was established from the ascending aorta, and circulatory arrest with deep hypothermia and retrograde cerebral perfusion were used for brain protection during surgery for thoracic aortic arch aneurysm. Hospital mortality was 12.5% (3/24 cases). The causes of death were cerebral infarction and respiratory failure. Antegrade systemic perfusion and aortic no-touch technique were an effective method of surgery for thoracic aortic aneurysm with abdominal aortic aneurysm to avoid perioperative embolism and major complications. We successfully performed staged operation, but regular radiographic follow-up was needed.