3.The significance and problems of medical interview training with standardized patients in clinical training after objective structured clinical examinations
Hisashi MASUGATA ; Hiroki OKADA ; Yukiko YOSHIMA ; Fuminori GODA ; Takeaki KOHNO ; Ayumu YAMAGAMI ; Hiroyuki OKUYAMA ; Naosi HIMOTO ; Shoichi SENDA
Medical Education 2009;40(3):175-179
1) To examine the significance and problems of medical interview training, self-administered questionnaires were completed by 90 students who took part in medical interview training with simulated patients in clinical training after objective structured clinical examinations.2) Most students thought that medical interview training was meaningful and thanked the simulated patients for their useful comments. On the other hand, some students felt that to improve their interviewing skills stricter feedback from simulated patients and instructors might be needed.3) Our results suggest that repeated training in medical interviewing in clinical training after objective structured clinical examinations is an effective method for teaching communication skills. However, in the future the scenarios should be improved and the frequency of medical interview training should be increased.
4.Effect of Cryopreservation of Human Heart Cells on Cell Proliferation
Hiroki Yokomuro ; Noritsugu Shiono ; Tsukasa Ozawa ; Takeshirou Fujii ; Muneyasu Kawasaki ; Yoshinori Watanabe ; Katsunori Yoshihara ; Nobuya Koyama ; Mitsumasa Okada
Japanese Journal of Cardiovascular Surgery 2006;35(1):14-20
Preservation is essential for successful cell transplantation. 1) Control group (n=13); Cells isolated from human right atrial tissues were cultured for 15 days. 2) Cell-cryopreservation (C. P.) group (n=23), Tissue-C. P. group (n=29); Human heart cells and minced tissues were cryopreserved in freezing medium containing 70% IMDM, 20% FBS, and 10% DMSO at a rate of 1°C/min. to -80°C by a programmed freezer and stored in liquid nitrogen (-196°C) for 1 week. After cryapreservation, the tissues and cells were thawed rapidly at 37°C. The cells, cryopreserved cells and cells isolated from cryopreserved tissues were cultured as passage 1, 2, and 3 for 15 days each. Cell proliferation was compared with a control group by determining growth curves, and 2-day proliferation rates. A growth factor, biochemical features and cell cycle were measured pre and post-cryopreservation. The cryopreserved group proliferated much more than the control group within 15 days at passage 1, 2, and 3 (1.7, 2.1, and 3.1 times, p<0.0001) respectively. The 2-day proliferation rates of cryopreservation group were higher than the control group in 15 days (p<0.05). The bFGF release after cryopreservation was on average 46.8 and 6.8 times greater than before cryopreservation for the Cell-C. P. and Tissue-C. P. groups, respectively. The TGF-β1 release was also accelerated by cryopreservation (Cell-C.P. group: 1.78 times, Tissue-C. P. group: 1.45 times in average) after cryopreservation. The cell cycle of human heart cells shifted to G2+M from the G1+G0 period by cryopreservation. Human atrial tissues and cells can be cultured and cryopreserved. The cryopreserved cells and cells isolated from cryopreserved tissue proliferate much more than non-cryopreserved cells at all cell ages. Cryopreservation enables human tissues and cells to proliferate more because of the greater release of growth factors and changing cell cycle.
5.Laboratory practice in transfusion medicine for medical students and physicians at Okayama University Hospital
Kazuma IKEDA ; Haruko SUGIYAMA ; Tohru IKEDA ; Naomi ASANO ; Hiroaki OGO ; Tomoko MIYOSHI ; Hitomi KATAOKA ; Takaaki MIZUSHIMA ; Yoshio NAKAMURA ; Nobuchika KUSANO ; Hiroki OKADA ; Koji OCHI ; Norio KOIDE
Medical Education 2010;41(1):51-53
1) All students but 1 correctly typed the ABO blood groups, but only 33.2% of students and 63.9% of physicians properly performed cross-matching.
2) Most failures in cross-matching were due to the inability to detect allogeneic antibodies, but 5.2% of students and 2.9% of physicians failed to detect ABO mismatching.
3) Although laboratory practice is suggested to help students to solidify knowledge and comprehend principles, achieving an official goal of residency - gaining competence in performing and interpreting cross-matching independently - appeared difficult.