2.Stress- and Aging-Associated Modulation of Macrophage Functions
Takako KIZAKI ; Kenji SUZUKI ; Tomomi OOKAWARA ; Tetsuya IZAWA ; Daizoh SAITOH ; Shuji OH-ISHI ; Keiichiro SUZUKI ; Shukoh HAGA ; Hideki OHNO
Environmental Health and Preventive Medicine 2001;6(4):218-228
Effects of environmental (cold) stress and aging on cells in monocyte/macrophage lineage were investigated. We demonstrated that immune suppressive states seen in acute cold-stressed mice (8-10 weeks of age) is attributable to FcγRIIbright suppressor macrophages. Serum corticosterone levels were markedly increased in acute cold-stressed mice. In addition, expression of glucocorticoids (GC) receptor mRNA was observed in FcγRIIbright cells from these mice. The increase of FcγRIIbright cells in peritoneal exudate cells caused by acute cold stress was inhibited by adrenalectomy or administration of a saturating amount of the GC antagonist RU 38486 (mifepristone). On the contrary, administration of the GC agonist, dexamethasone, markedly increased the proportion of FcγRIIbright cells in peritoneal exudate cells of control mice. These results suggest that the generation of FcγRIIbright suppressor cells of monocyte/macrophage lineage by acute cold stress was mediated by action of GC through the GC receptor. We likewise found that the proportion of FcγRIIbright suppressor macrophages is increased in aged mice (22-24 months of age). Meanwhile, activated macrophages which function as antigen presenting cells were decreased in aged rats. Both the basal corticosterone concentrations in serum and the expression of mRNA for GC receptor in peritoneal macrophages increased significantly in aged animals, suggesting that these populational and functional changes of macrophages in aged animals were mediated, in part, by the increased basal levels of GC. This is probably being responsible for immunosenescence.
Neisseria gonorrhoeae
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Laboratory mice
;
Acute
;
Macrophages
;
receptor
3.The experimental system to analyze mRNA expression profiles between slow and fast muscle fibers
Yoshiaki Hitomi ; Takako Kizaki ; Norihiko Nakano ; Toshihito Katsumura ; Kazuki Esaki ; Tohru Takemasa ; Takashi Kinugasa ; Shukoh Haga ; Tetsuya Izawa ; Hideki Ohno
Japanese Journal of Physical Fitness and Sports Medicine 2005;54(1):73-73
4.A case of Post-operative Complication and Chronic Pain Successfully Treated with Kampo Medicine for Qi Disturbance
Yumiko KIMATA ; Nobuyasu SEKIYA ; Yuji KASAHARA ; Atsushi CHINO ; Yoshiro HIRASAKI ; Keiko OGAWA ; Hideki OKAMOTO ; Keigo UEDA ; Kenji OHNO ; Takao NAMIKI ;
Kampo Medicine 2011;62(1):48-52
We experienced a case of post-operative complication and chronic pain due to left pyeloplasty and uterine myomectomy successfully treated with Kampo medicine. A 55-year-old woman underwent pyeloplasty for ureteropelvic junction obstruction, and ureteral stent for ureteral stenosis caused by synechia after uterine myomectomy. She suffered from post-operative complication and chronic pain, which was becoming severe, and had a depressed mood. She visited our outpatient clinic for Kampo therapy. We prescribed bukuryoingohangekobokuto on the diagnoses such as qi deficiency, qi stagnation, and water retention. Her symptoms disappeared with this formulation. This case suggests the importance of considering qi disturbance when we treat patients with chronic pain using Kampo medicine.
5.Combined laparoscopic and transperineal endoscopic total pelvic exenteration for the vaginal stump recurrence of cervical cancer
Ryo OHNO ; Yoshiko MATSUMOTO ; Hideki NAGANO ; Akira KOMONO ; Naoya AISU ; Gumpei YOSHIMATSU ; Suguru HASEGAWA
Journal of Gynecologic Oncology 2022;33(1):e16-
Total pelvic exenteration (TPE) is sometimes required for radical treatment of locally advanced or recurrent gynecologic cancer [1]. However, TPE with a transabdominal approach requires highly advanced techniques in the case of repeated surgery due to the effects of primary surgery and/or chemoradiotherapy, especially when a transabdominal approach is used. Recent technical advances in transanal/transperineal endoscopic surgery have proved beneficial for complicated surgery in the deep pelvis [2]. Here we introduce our surgical procedure for combined laparoscopic and transperineal endoscopic TPE (TpTPE) for pelvic recurrence of cervical cancer. A 42-year-old woman was diagnosed with vaginal stump recurrence of cervical cancer involving the rectum, bladder, and ureters following hysterectomy and pelvic lymph node dissection as primary surgery and chemotherapy/chemoradiotherapy for previous recurrences. We decided to perform TpTPE with a combined laparoscopic approach. The GelPOINT advanced access platform was fixed through a perineal skin incision around the tightly closed anus, external urethral orifice, and vagina. With sufficient pneumopelvic pressure (12 mmHg), TpTPE was performed under a good surgical view without any effect of the primary surgery. A ureterostomy and sigmoid colostomy were created and a right gracilis muscle flap was used to reconstruct the pelvic defect. The total operative time and estimated blood loss were 887 minutes and 497 mL, respectively. Histopathological examination revealed recurrent cervical cancer invading the rectum, bladder, and bilateral ureters with negative surgical margins. The postoperative course was uneventful except for paralytic ileus. The patient was discharged on postoperative day 18. TpTPE is a technically feasible and effective approach for locally advanced pelvic tumors.
6.The Evaluation of Handy Objective Indicator of Skin Moisture and Its Clinical Usefulness in Kampo Medicine
Takao NAMIKI ; Nobuyasu SEKIYA ; Yuji KASAHARA ; Atsushi CHINO ; Katsumi HAYASHI ; Yoshiro HIRASAKI ; Kenji OHNO ; Yukitaka HIYAMA ; Toshiaki KITA ; Hideki HAYASHI ; Katsutoshi TERASAWA
Kampo Medicine 2007;58(6):1113-1119
Objects : The purpose of this study is to evaluate objective measurements of skin moisture and its usefulness in the Kampo clinic.Methods : We measured the skin moisture of 6 male volunteers using a sensor twice, in a one to two week interval, and calculated the correlation of both data sets at 66 points. Secondly, we compared the skin moisture data at 10 regions in 81 patients without any skin disease, in a wide age range from 30 s to early 70 s. The ten regions measured were : face, chest, back, abdomen, leg and 4 forearm points, i.e. the inside and outside of the bilateral center forearm, and palm.Results : The correlation of two successively measured data sets showed excellent relation (r=0.716, p<0.0001). The sensor measurement of skin moisture was quite reliable. Measurements of the face, chest and back showed them to be significantly wetter than the abdomen, leg or any of the 4 forearm points. Palm measurements indicated a wetter tendency than for any forearm measurements. Back and abdomen measurements in males were significantly wetter than those in females. There were no significant differences among the data sets, with regards to age. Therefore, any forearm points are clinically useful for the standard measurement of skin moisture. We also demonstrated a case in which skin moisture measurements with a sensor were useful in prescribing and observing the effects of shigyakusan.Conclusion : Utilizing this method may be helpful in acquiring much more objective measurements for Kampo medicine.
Integumentary system
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seconds
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Forearm
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Medicine, Kampo
;
Measurement
7.Efficacy of Kampo Medicine for Migraine in Children and Childhood Periodic Syndromes
Masaki RAIMURA ; Takao NAMIKI ; Nobuyasu SEKIYA ; Yuji KASAHARA ; Atsushi CHINO ; Yoshiro HIRASAKI ; Keiko OGAWA ; Hirokuni OKUMI ; Hideki OKAMOTO ; Yumiko KIMATA ; Keigo UEDA ; Takeshi OUJI ; Kenji OHNO ; Satoshi YAMAMOTO ; Tetsuo AKIBA ; Katsutoshi TERASAWA
Kampo Medicine 2011;62(4):574-583
In the Guideline for treatment of chronic headache published by Japanese Headache Society, Ibuprofen and Acetaminophen are proposed for the acute treatment of migraine in children. But prophylactic treatment of pediatric migraine is not established. We report the efficacy of Kampo medicine for preventing migraine in children and childhood periodic syndromes. We use a variety of Kampo medicine for 9 pediatric migraine and periodic syndromes patients from 8 to 15 years old. All 9 patients improve their headache and associated symptoms including abdominal pain, vertigo, nausea and vomiting. After treatment the mean average of Headache Impact Test-6 (HIT-6) improve from 63.66 points to 45.77 points. Kampo medicines is effective for migraine in children and childhood periodic syndromes.
8.Impact of sarcopenia on biliary drainage during neoadjuvant therapy for pancreatic cancer
Kunio KATAOKA ; Eizaburo OHNO ; Takuya ISHIKAWA ; Kentaro YAMAO ; Yasuyuki MIZUTANI ; Tadashi IIDA ; Hideki TAKAMI ; Osamu MAEDA ; Junpei YAMAGUCHI ; Yukihiro YOKOYAMA ; Tomoki EBATA ; Yasuhiro KODERA ; Hiroki KAWASHIMA
Clinical Endoscopy 2024;57(1):112-121
Background/Aims:
Since the usefulness of neoadjuvant chemo(radiation) therapy (NAT) for pancreatic cancer has been demonstrated, recurrent biliary obstruction (RBO) in patients with pancreatic cancer with a fully covered self-expandable metal stent (FCSEMS) during NAT is expected to increase. This study investigated the impact of sarcopenia on RBO in this setting.
Methods:
Patients were divided into normal and low skeletal muscle index (SMI) groups and retrospectively analyzed. Patient characteristics, overall survival, time to RBO (TRBO), stent-related adverse events, and postoperative complications were compared between the two groups. A Cox proportional hazard model was used to identify the risk factors for short TRBO.
Results:
A few significant differences were observed in patient characteristics, overall survival, stent-related adverse events, and postoperative complications between 38 patients in the normal SMI group and 17 in the low SMI group. The median TRBO was not reached in the normal SMI group and was 112 days in the low SMI group (p=0.004). In multivariate analysis, low SMI was the only risk factor for short TRBO, with a hazard ratio of 5.707 (95% confidence interval, 1.148–28.381; p=0.033).
Conclusions
Sarcopenia was identified as an independent risk factor for RBO in patients with pancreatic cancer with FCSEMS during NAT.
9.Stress- and aging-associated modulation of macrophage functions.
Takako KIZAKI ; Kenji SUZUKI ; Tomomi OOKAWARA ; Tetsuya IZAWA ; Daizoh SAITOH ; Shuji OH-ISHI ; Keiichiro SUZUKI ; Shukoh HAGA ; Hideki OHNO
Environmental Health and Preventive Medicine 2002;6(4):218-228
Effects of environmental (cold) stress and aging on cells in monocyte/macrophage lineage were investigated. We demonstrated that immune suppressive states seen in acute cold-stressed mice (8-10 weeks of age) is attributable to FcγRII(bright) suppressor macrophages. Serum corticosterone levels were markedly increased in acute cold-stressed mice. In addition, expression of glucocorticoids (GC) receptor mRNA was observed in FcγRII(bright) cells from these mice. The increase of FcγRII(bright) cells in peritoneal exudate cells caused by acute cold stress was inhibited by adrenalectomy or administration of a saturating amount of the GC antagonist RU 38486 (mifepristone). On the contrary, administration of the GC agonist, dexamethasone, markedly increased the proportion of FcγRII(bright) cells in peritoneal exudate cells of control mice. These results suggest that the generation of FcγRII(bright) suppressor cells of monocyte/macrophage lineage by acute cold stress was mediated by action of GC through the GC receptor. We likewise found that the proportion of FcγRII(bright) suppressor macrophages is increased in aged mice (22-24 months of age). Meanwhile, activated macrophages which function as antigen presenting cells were decreased in aged rats. Both the basal corticosterone concentrations in serum and the expression of mRNA for GC receptor in peritoneal macrophages increased significantly in aged animals, suggesting that these populational and functional changes of macrophages in aged animals were mediated, in part, by the increased basal levels of GC. This is probably being responsible for immunosenescence.
10.Mitral Valve Repair for Failed MitraClip: a Case Report
Shintaro KUWAUCHI ; Mitsuharu HOSONO ; Tomohiko UETSUKI ; Masato OHNO ; Hideki SAKASHITA ; Takayuki OKADA ; Nobuya ZEMPO ; Naoki MINATO ; Kohei KAWAZOE
Japanese Journal of Cardiovascular Surgery 2023;52(6):396-400
The patient was an 89-year-old male who underwent transcatheter edge-to-edge repair to the mitral valve using MitraClip for severe degenerative mitral regurgitation (MR) one year earlier. Although two clips were implanted, grade III/IV MR still remained. As his heart failure progressed, he was referred to us for surgery. The patient also had aortic stenosis. He underwent mitral valve repair and aortic valve replacement. The postoperative course was uneventful. He was transferred to the referring hospital on postoperative day 14. When performing MitraClip for degenerative MR, it is important to consider carefully not only the operative risk for open surgery but also the anatomical adequacy of MitraClip. When MitraClip fails to control MR, early surgical intervention should be considered.